(Circular) (Six-base) MAPSORT of: pFN21AB7785.seq Check: 4516 from: 1 to: 6076 pFN21AB7785 6076 bp With 182 enzymes: SgfI * October 20, 2009 10:55 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3886 278 Size: 53 83 186 936 2350 2468 Fragments arranged by size: 2468 2350 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2518 3419 1937 Size: 581 901 4594 Fragments arranged by size: 4594 901 581 Acc65I G'GTAC_C Cuts at: 3397 3397 Size: 6076 AclI AA'CG_TT Cuts at: 1563 4203 4576 1563 Size: 2640 373 3063 Fragments arranged by size: 3063 2640 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2057 3169 3253 4139 5523 5791 1632 Size: 425 1112 84 886 1384 268 1917 Fragments arranged by size: 1917 1384 1112 886 425 268 84 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5879 AflIII A'CryG_T Cuts at: 1203 4975 5952 1203 Size: 3772 977 1327 Fragments arranged by size: 3772 1327 977 AgeI A'CCGG_T Cuts at: 4907 4907 Size: 6076 AhdI GACnn_n'nnGTC Cuts at: 4805 4805 Size: 6076 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6076 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6076 AlwNI CAG_nnn'CTG Cuts at: 3819 5391 3819 Size: 1572 4504 ApaLI G'TGCA_C Cuts at: 4133 5289 4133 Size: 1156 4920 ApoI r'AATT_y Cuts at: 1493 2322 2799 3051 3337 3385 3611 3763 5872 Size: 829 477 252 286 48 226 152 2109 Cuts at: 5872 1493 Size: 1697 Fragments arranged by size: 2109 1697 829 477 286 252 226 152 48 AseI AT'TA_AT Cuts at: 160 1032 4630 160 Size: 872 3598 1606 Fragments arranged by size: 3598 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6076 AvaI C'yCGr_G Cuts at: 1870 1943 3401 1870 Size: 73 1458 4545 Fragments arranged by size: 4545 1458 73 BamHI G'GATC_C Cuts at: 3406 3773 3406 Size: 367 5709 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3397 4852 618 Size: 325 217 627 1610 1455 1842 Fragments arranged by size: 1842 1610 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3281 3395 1391 Size: 1890 114 4072 Fragments arranged by size: 4072 1890 114 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5105 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4267 4301 1903 Size: 34 2330 34 3678 Fragments arranged by size: 3678 2330 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3968 5178 3968 Size: 1210 4866 BclI T'GATC_A Cuts at: 1548 2310 1548 Size: 762 5314 BfrBI ATG'CAT Cuts at: 2573 2913 3113 2573 Size: 340 200 5536 Fragments arranged by size: 5536 340 200 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4687 136 Size: 107 122 71 631 605 3015 1525 Fragments arranged by size: 3015 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2224 6071 2224 Size: 3847 2229 BlpI GC'TnA_GC Cuts at: 3493 3493 Size: 6076 Bme1580I G_kGCm'C Cuts at: 1790 4137 5293 1790 Size: 2347 1156 2573 Fragments arranged by size: 2573 2347 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6076 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2147 2296 2733 Size: 416 53 83 399 24 730 149 437 Cuts at: 2733 3807 4765 442 Size: 1074 958 1753 Fragments arranged by size: 1753 1074 958 730 437 416 399 149 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6076 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3086 3086 Size: 6076 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2577 2913 3081 3165 3249 3295 4736 1239 Size: 1338 336 168 84 84 46 1441 2579 Fragments arranged by size: 2579 1441 1338 336 168 84 84 46 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4201 5066 5364 4201 Size: 865 298 4913 Fragments arranged by size: 4913 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2137 4739 882 Size: 617 638 2602 2219 Fragments arranged by size: 2602 2219 638 617 BsaAI yAC'GTr Cuts at: 493 1204 3867 493 Size: 711 2663 2702 Fragments arranged by size: 2702 2663 711 BsaBI GATnn'nnATC Cuts at: 6070 6070 Size: 6076 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3883 4265 4900 275 Size: 53 83 186 936 2350 382 635 1451 Fragments arranged by size: 2350 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4509 4907 5181 5328 5739 4509 Size: 398 274 147 411 4846 Fragments arranged by size: 4846 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5851 Fragments arranged by size: 5851 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2072 1186 Size: 886 5190 BseYI C'CCAG_C Cuts at: 2200 5279 5775 2200 Size: 3079 496 2501 Fragments arranged by size: 3079 2501 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5503 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3526 4287 5315 1540 Size: 275 186 1525 761 1028 2301 Fragments arranged by size: 2301 1525 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3281 4137 4222 5293 3281 Size: 856 85 1071 4064 Fragments arranged by size: 4064 1071 856 85 BsmI GAATG_Cn' Cuts at: 2915 3587 3680 2915 Size: 672 93 5311 Fragments arranged by size: 5311 672 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3281 3395 4137 4222 5293 1391 Size: 399 1491 114 742 85 1071 2174 Fragments arranged by size: 2174 1491 1071 742 399 114 85 BspEI T'CCGG_A Cuts at: 5739 5739 Size: 6076 BspHI T'CATG_A Cuts at: 3965 5695 3965 Size: 1730 4346 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3431 844 Size: 2587 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3963 1394 Size: 429 2140 3507 Fragments arranged by size: 3507 2140 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4571 4745 65 Size: 1183 3323 174 1396 Fragments arranged by size: 3323 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4720 4907 1700 Size: 110 2910 187 2869 Fragments arranged by size: 2910 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2094 96 Size: 1998 4078 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6076 BssSI C'ACGA_G Cuts at: 4136 5148 4136 Size: 1012 5064 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6076 BstBI TT'CG_AA Cuts at: 4880 4880 Size: 6076 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6076 BstXI CCAn_nnnn'nTGG Cuts at: 2501 2501 Size: 6076 BstYI r'GATC_y Cuts at: 1972 2224 3406 3773 4158 4175 5627 5713 5725 Size: 252 1182 367 385 17 1452 86 12 Cuts at: 5725 6071 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1182 385 367 346 252 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5524 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5337 Fragments arranged by size: 5337 683 56 BtsI GCAGTG_nn' Cuts at: 2860 3592 4386 4406 2860 Size: 732 794 20 4530 Fragments arranged by size: 4530 794 732 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6076 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6041 DraI TTT'AAA Cuts at: 2356 3380 2356 Size: 1024 5052 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5083 809 Size: 507 3767 1802 Fragments arranged by size: 3767 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2037 2122 3523 Size: 54 1425 50 275 18 207 85 1401 Cuts at: 3523 4412 6041 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1401 889 275 207 85 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3523 1537 Size: 275 1711 4090 Fragments arranged by size: 4090 1711 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3128 4006 1353 Size: 1775 878 3423 Fragments arranged by size: 3423 1775 878 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4663 5037 5183 387 Size: 1064 138 3074 374 146 1280 Fragments arranged by size: 3074 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3279 3279 Size: 6076 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2057 2577 2913 3081 3165 3169 3249 Size: 393 425 520 336 168 84 4 80 Cuts at: 3249 3253 3295 4139 4736 5523 5791 1239 Size: 4 42 844 597 787 268 1524 Fragments arranged by size: 1524 844 787 597 520 425 393 336 268 168 84 80 42 4 4 EcoO109I rG'GnC_Cy Cuts at: 2336 2481 2336 Size: 145 5931 EcoRI G'AATT_C Cuts at: 2322 2799 3051 3385 3763 2322 Size: 477 252 334 378 4635 Fragments arranged by size: 4635 477 378 334 252 FspI TGC'GCA Cuts at: 4582 4582 Size: 6076 HaeII r_GCGC'y Cuts at: 1678 5223 1678 Size: 3545 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2282 2314 3295 3327 4717 Size: 32 135 32 710 32 981 32 1390 Cuts at: 4717 4749 4791 4823 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1390 981 710 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2519 3420 3666 669 Size: 988 281 581 901 246 3079 Fragments arranged by size: 3079 988 901 581 281 246 HindIII A'AGCT_T Cuts at: 748 3012 748 Size: 2264 3812 HpaI GTT'AAC Cuts at: 3666 3666 Size: 6076 KpnI G_GTAC'C Cuts at: 3401 3401 Size: 6076 MfeI C'AATT_G Cuts at: 3675 3675 Size: 6076 MluI A'CGCG_T Cuts at: 5952 5952 Size: 6076 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2091 2802 5190 5374 1305 Size: 786 711 2388 184 2007 Fragments arranged by size: 2388 2007 786 711 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2039 2124 10 Size: 54 1425 343 207 85 3962 Fragments arranged by size: 3962 1425 343 207 85 54 MslI CAynn'nnrTG Cuts at: 518 1166 2499 2572 4034 4393 4552 518 Size: 648 1333 73 1462 359 159 2042 Fragments arranged by size: 2042 1462 1333 648 359 159 73 MspA1I CmG'CkG Cuts at: 1606 3273 3492 4169 5317 5562 1606 Size: 1667 219 677 1148 245 2120 Fragments arranged by size: 2120 1667 1148 677 245 219 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5524 NdeI CA'TA_TG Cuts at: 387 2457 2569 387 Size: 2070 112 3894 Fragments arranged by size: 3894 2070 112 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6076 NotI GC'GGCC_GC Cuts at: 3523 3523 Size: 6076 NsiI A_TGCA'T Cuts at: 2575 2915 3115 2575 Size: 340 200 5536 Fragments arranged by size: 5536 340 200 NspI r_CATG'y Cuts at: 1132 1229 2577 3113 3434 4979 1132 Size: 97 1348 536 321 1545 2229 Fragments arranged by size: 2229 1545 1348 536 321 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6076 PciI A'CATG_T Cuts at: 4975 4975 Size: 6076 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6076 PmeI GTTT'AAAC Cuts at: 3380 3380 Size: 6076 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4145 4177 5658 5690 4145 Size: 32 1481 32 4531 Fragments arranged by size: 4531 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6076 PsiI TTA'TAA Cuts at: 3646 3646 Size: 6076 PstI C_TGCA'G Cuts at: 830 3428 830 Size: 2598 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6076 PvuII CAG'CTG Cuts at: 3273 3273 Size: 6076 SacI G_AGCT'C Cuts at: 3281 3281 Size: 6076 SalI G'TCGA_C Cuts at: 1936 2517 3418 1936 Size: 581 901 4594 Fragments arranged by size: 4594 901 581 SbfI CC_TGCA'GG Cuts at: 3428 3428 Size: 6076 ScaI AGT'ACT Cuts at: 2470 4324 6036 2470 Size: 1854 1712 2510 Fragments arranged by size: 2510 1854 1712 SexAI A'CCwGG_T Cuts at: 3372 3372 Size: 6076 SfcI C'TryA_G Cuts at: 826 1045 3424 4559 5240 5431 826 Size: 219 2379 1135 681 191 1471 Fragments arranged by size: 2379 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6076 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6076 SmaI CCC'GGG Cuts at: 1872 3403 1872 Size: 1531 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4180 5081 5343 820 Size: 197 926 2237 901 262 1553 Fragments arranged by size: 2237 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3867 493 Size: 3374 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6076 SphI G_CATG'C Cuts at: 1132 3113 3434 1132 Size: 1981 321 3774 Fragments arranged by size: 3774 1981 321 SspI AAT'ATT Cuts at: 5 52 4000 5 Size: 47 3948 2081 Fragments arranged by size: 3948 2081 47 StuI AGG'CCT Cuts at: 1517 2762 1517 Size: 1245 4831 StyI C'CwwG_G Cuts at: 513 1065 3515 513 Size: 552 2450 3074 Fragments arranged by size: 3074 2450 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4103 4120 4273 4458 4903 325 Size: 1255 2523 17 153 185 445 1498 Fragments arranged by size: 2523 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2094 2468 2744 Size: 274 80 33 51 1443 117 374 276 Cuts at: 2744 4322 6034 96 Size: 1578 1712 138 Fragments arranged by size: 1712 1578 1443 374 276 274 138 117 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6036 6068 6036 Size: 32 6044 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6076 XbaI T'CTAG_A Cuts at: 3412 3412 Size: 6076 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6076 XmaI C'CCGG_G Cuts at: 1870 3401 1870 Size: 1531 4545 XmnI GAAnn'nnTTC Cuts at: 2322 2868 3014 4205 2322 Size: 546 146 1191 4193 Fragments arranged by size: 4193 1191 546 146 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3884 276 Size: 53 83 186 936 2350 2468 Fragments arranged by size: 2468 2350 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI