(Circular) (Six-base) MAPSORT of: pFN21AB9409.seq Check: 5488 from: 1 to: 5572 pFN21AB9409 5572 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3382 278 Size: 53 83 186 936 1846 2468 Fragments arranged by size: 2468 1846 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2915 1937 Size: 978 4594 Acc65I G'GTAC_C Cuts at: 2893 2893 Size: 5572 AclI AA'CG_TT Cuts at: 1563 3699 4072 1563 Size: 2136 373 3063 Fragments arranged by size: 3063 2136 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3635 5019 5287 1632 Size: 2003 1384 268 1917 Fragments arranged by size: 2003 1917 1384 268 AfeI AGC'GCT Cuts at: 2111 2111 Size: 5572 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5375 AflIII A'CryG_T Cuts at: 1203 4471 5448 1203 Size: 3268 977 1327 Fragments arranged by size: 3268 1327 977 AgeI A'CCGG_T Cuts at: 4403 4403 Size: 5572 AhdI GACnn_n'nnGTC Cuts at: 4301 4301 Size: 5572 AleI CACnn'nnGTG Cuts at: 1166 2720 1166 Size: 1554 4018 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2063 1184 Size: 879 4693 AlwNI CAG_nnn'CTG Cuts at: 2092 2410 3315 4887 2092 Size: 318 905 1572 2777 Fragments arranged by size: 2777 1572 905 318 ApaLI G'TGCA_C Cuts at: 3629 4785 3629 Size: 1156 4416 ApoI r'AATT_y Cuts at: 1493 2881 3107 3259 5368 1493 Size: 1388 226 152 2109 1697 Fragments arranged by size: 2109 1697 1388 226 152 AseI AT'TA_AT Cuts at: 160 1032 4126 160 Size: 872 3094 1606 Fragments arranged by size: 3094 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5572 AvaI C'yCGr_G Cuts at: 1870 1943 2897 1870 Size: 73 954 4545 Fragments arranged by size: 4545 954 73 BamHI G'GATC_C Cuts at: 2807 2902 3269 2807 Size: 95 367 5110 Fragments arranged by size: 5110 367 95 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2893 4348 618 Size: 325 217 627 1106 1455 1842 Fragments arranged by size: 1842 1455 1106 627 325 217 BanII G_rGCy'C Cuts at: 1391 2646 2669 2891 1391 Size: 1255 23 222 4072 Fragments arranged by size: 4072 1255 222 23 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2224 928 Size: 971 325 4276 Fragments arranged by size: 4276 971 325 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3763 3797 1903 Size: 34 1826 34 3678 Fragments arranged by size: 3678 1826 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3464 4674 3464 Size: 1210 4362 BclI T'GATC_A Cuts at: 1548 1548 Size: 5572 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4183 136 Size: 107 122 71 631 605 2511 1525 Fragments arranged by size: 2511 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5567 5567 Size: 5572 BlpI GC'TnA_GC Cuts at: 2989 2989 Size: 5572 Bme1580I G_kGCm'C Cuts at: 1790 3633 4789 1790 Size: 1843 1156 2573 Fragments arranged by size: 2573 1843 1156 BmgBI CAC'GTC Cuts at: 1318 2722 2854 1318 Size: 1404 132 4036 Fragments arranged by size: 4036 1404 132 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3303 4261 442 Size: 416 53 83 399 24 1886 958 1753 Fragments arranged by size: 1886 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5572 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2216 2435 2630 4232 1239 Size: 977 219 195 1602 2579 Fragments arranged by size: 2579 1602 977 219 195 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3697 4562 4860 3697 Size: 865 298 4409 Fragments arranged by size: 4409 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2339 4235 882 Size: 617 840 1896 2219 Fragments arranged by size: 2219 1896 840 617 BsaAI yAC'GTr Cuts at: 493 1204 3363 493 Size: 711 2159 2702 Fragments arranged by size: 2702 2159 711 BsaBI GATnn'nnATC Cuts at: 5566 5566 Size: 5572 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3379 3761 4396 275 Size: 53 83 186 936 1846 382 635 1451 Fragments arranged by size: 1846 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4005 4403 4677 4824 5235 4005 Size: 398 274 147 411 4342 Fragments arranged by size: 4342 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2000 2030 2357 2387 2510 Size: 30 165 30 597 30 327 30 123 Cuts at: 2510 2540 1178 Size: 30 4210 Fragments arranged by size: 4210 597 327 165 123 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2120 2460 2756 1186 Size: 934 340 296 4002 Fragments arranged by size: 4002 934 340 296 BseYI C'CCAG_C Cuts at: 2270 2434 2508 4775 5271 2270 Size: 164 74 2267 496 2571 Fragments arranged by size: 2571 2267 496 164 74 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2165 1163 Size: 573 429 4570 Fragments arranged by size: 4570 573 429 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2077 3022 3783 4811 1540 Size: 275 186 76 945 761 1028 2301 Fragments arranged by size: 2301 1028 945 761 275 186 76 BsiHKAI G_wGCw'C Cuts at: 2129 2490 2669 2766 3633 3718 4789 2129 Size: 361 179 97 867 85 1071 2912 Fragments arranged by size: 2912 1071 867 361 179 97 85 BsmI GAATG_Cn' Cuts at: 3083 3176 3083 Size: 93 5479 BsmBI CGTCTCn'nnnn_ Cuts at: 2064 2064 Size: 5572 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2129 2490 2646 2669 2766 2891 3633 Size: 399 339 361 156 23 97 125 742 Cuts at: 3633 3718 4789 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 361 339 156 125 97 85 23 BspEI T'CCGG_A Cuts at: 5235 5235 Size: 5572 BspHI T'CATG_A Cuts at: 2473 3461 5191 2473 Size: 988 1730 2854 Fragments arranged by size: 2854 1730 988 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2070 2100 2927 844 Size: 1226 30 827 3489 Fragments arranged by size: 3489 1226 827 30 BsrBI CCG'CTC Cuts at: 1394 1823 3459 1394 Size: 429 1636 3507 Fragments arranged by size: 3507 1636 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2556 4067 4241 65 Size: 1183 1308 1511 174 1396 Fragments arranged by size: 1511 1396 1308 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4216 4403 1700 Size: 110 2406 187 2869 Fragments arranged by size: 2869 2406 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5572 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5572 BssSI C'ACGA_G Cuts at: 2021 3632 4644 2021 Size: 1611 1012 2949 Fragments arranged by size: 2949 1611 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2092 2494 794 Size: 1298 402 3872 Fragments arranged by size: 3872 1298 402 BstBI TT'CG_AA Cuts at: 4376 4376 Size: 5572 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5572 BstYI r'GATC_y Cuts at: 1972 2807 2902 3269 3654 3671 5123 5209 5221 Size: 835 95 367 385 17 1452 86 12 Cuts at: 5221 5567 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 835 385 367 346 95 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2403 2403 Size: 5572 BtgI C'CryG_G Cuts at: 513 1065 2004 2361 513 Size: 552 939 357 3724 Fragments arranged by size: 3724 939 552 357 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4833 Fragments arranged by size: 4833 683 56 BtsI GCAGTG_nn' Cuts at: 2129 3088 3882 3902 2129 Size: 959 794 20 3799 Fragments arranged by size: 3799 959 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5572 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5537 DraI TTT'AAA Cuts at: 2876 2876 Size: 5572 DraIII CAC_nnn'GTG Cuts at: 2026 2026 Size: 5572 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2078 2287 4579 809 Size: 507 762 209 2292 1802 Fragments arranged by size: 2292 1802 762 507 209 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2181 2241 3019 Size: 54 1425 50 275 18 351 60 778 Cuts at: 3019 3908 5537 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 778 351 275 60 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3019 1537 Size: 275 1207 4090 Fragments arranged by size: 4090 1207 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2220 3502 1353 Size: 867 1282 3423 Fragments arranged by size: 3423 1282 867 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4159 4533 4679 387 Size: 1064 138 2570 374 146 1280 Fragments arranged by size: 2570 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2667 2667 Size: 5572 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2216 2435 2630 3635 4232 5019 5287 Size: 393 584 219 195 1005 597 787 268 Cuts at: 5287 1239 Size: 1524 Fragments arranged by size: 1524 1005 787 597 584 393 268 219 195 EcoO109I rG'GnC_Cy Cuts at: 2275 2275 Size: 5572 EcoRI G'AATT_C Cuts at: 2881 3259 2881 Size: 378 5194 EcoRV GAT'ATC Cuts at: 2863 2863 Size: 5572 FspI TGC'GCA Cuts at: 2171 2498 4078 2171 Size: 327 1580 3665 Fragments arranged by size: 3665 1580 327 HaeII r_GCGC'y Cuts at: 1678 2113 4719 1678 Size: 435 2606 2531 Fragments arranged by size: 2606 2531 435 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2510 2542 2619 2651 4213 Size: 32 135 32 938 32 77 32 1562 Cuts at: 4213 4245 4287 4319 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1562 938 135 77 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2916 3162 669 Size: 988 281 978 246 3079 Fragments arranged by size: 3079 988 978 281 246 HindIII A'AGCT_T Cuts at: 748 2736 748 Size: 1988 3584 HpaI GTT'AAC Cuts at: 3162 3162 Size: 5572 KpnI G_GTAC'C Cuts at: 2897 2897 Size: 5572 MfeI C'AATT_G Cuts at: 3171 3171 Size: 5572 MluI A'CGCG_T Cuts at: 5448 5448 Size: 5572 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2182 2796 4686 4870 1305 Size: 877 614 1890 184 2007 Fragments arranged by size: 2007 1890 877 614 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2183 2243 10 Size: 54 1425 343 351 60 3339 Fragments arranged by size: 3339 1425 351 343 60 54 MslI CAynn'nnrTG Cuts at: 518 1166 2189 2720 3530 3889 4048 518 Size: 648 1023 531 810 359 159 2042 Fragments arranged by size: 2042 1023 810 648 531 359 159 MspA1I CmG'CkG Cuts at: 1606 2356 2363 2462 2515 2988 3665 4813 5058 Size: 750 7 99 53 473 677 1148 245 Cuts at: 5058 1606 Size: 2120 Fragments arranged by size: 2120 1148 750 677 473 245 99 53 7 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4081 Fragments arranged by size: 4081 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5572 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5572 NotI GC'GGCC_GC Cuts at: 3019 3019 Size: 5572 NspI r_CATG'y Cuts at: 1132 1229 2170 2323 2546 2930 4475 1132 Size: 97 941 153 223 384 1545 2229 Fragments arranged by size: 2229 1545 941 384 223 153 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5572 PciI A'CATG_T Cuts at: 4471 4471 Size: 5572 PflMI CCAn_nnn'nTGG Cuts at: 1108 2026 1108 Size: 918 4654 PmeI GTTT'AAAC Cuts at: 2876 2876 Size: 5572 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3641 3673 5154 5186 3641 Size: 32 1481 32 4027 Fragments arranged by size: 4027 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2275 2275 Size: 5572 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5572 PsiI TTA'TAA Cuts at: 3142 3142 Size: 5572 PstI C_TGCA'G Cuts at: 830 2088 2781 2924 830 Size: 1258 693 143 3478 Fragments arranged by size: 3478 1258 693 143 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5572 PvuII CAG'CTG Cuts at: 2462 2462 Size: 5572 RsrII CG'GwC_CG Cuts at: 2035 2035 Size: 5572 SacI G_AGCT'C Cuts at: 2669 2669 Size: 5572 SacII CC_GC'GG Cuts at: 2364 2364 Size: 5572 SalI G'TCGA_C Cuts at: 1936 2914 1936 Size: 978 4594 SanDI GG'GwC_CC Cuts at: 2275 2275 Size: 5572 SbfI CC_TGCA'GG Cuts at: 2924 2924 Size: 5572 ScaI AGT'ACT Cuts at: 3820 5532 3820 Size: 1712 3860 SexAI A'CCwGG_T Cuts at: 2868 2868 Size: 5572 SfcI C'TryA_G Cuts at: 826 1045 2084 2777 2847 2920 4055 4736 4927 Size: 219 1039 693 70 73 1135 681 191 Cuts at: 4927 826 Size: 1471 Fragments arranged by size: 1471 1135 1039 693 681 219 191 73 70 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5572 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5572 SmaI CCC'GGG Cuts at: 1872 2899 1872 Size: 1027 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3676 4577 4839 820 Size: 197 926 1733 901 262 1553 Fragments arranged by size: 1733 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3363 493 Size: 2870 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5572 SphI G_CATG'C Cuts at: 1132 2546 2930 1132 Size: 1414 384 3774 Fragments arranged by size: 3774 1414 384 SspI AAT'ATT Cuts at: 5 52 3496 5 Size: 47 3444 2081 Fragments arranged by size: 3444 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5572 StyI C'CwwG_G Cuts at: 513 1065 2004 3011 513 Size: 552 939 1007 3074 Fragments arranged by size: 3074 1007 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2297 3599 3616 3769 3954 4399 325 Size: 1255 717 1302 17 153 185 445 1498 Fragments arranged by size: 1498 1302 1255 717 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3818 5530 96 Size: 274 80 33 51 1443 1841 1712 138 Fragments arranged by size: 1841 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2000 2032 2201 2233 2357 2389 5532 5564 2000 Size: 32 169 32 124 32 3143 32 2008 Fragments arranged by size: 3143 2008 169 124 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2033 2078 1316 Size: 717 45 4810 Fragments arranged by size: 4810 717 45 XbaI T'CTAG_A Cuts at: 2908 2908 Size: 5572 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2191 2251 2719 2191 Size: 60 468 5044 Fragments arranged by size: 5044 468 60 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5572 XmaI C'CCGG_G Cuts at: 1870 2897 1870 Size: 1027 4545 XmnI GAAnn'nnTTC Cuts at: 3701 3701 Size: 5572 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3380 276 Size: 53 83 186 936 1846 2468 Fragments arranged by size: 2468 1846 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI SapI SfiI SfoI SrfI SwaI