Restriction map for
pFN21AB9097
(Circular) (Six-base) MAPSORT of: pFN21AB9097.seq Check: 3039 from: 1 to: 8803
pFN21AB9097 8803 bp
With 182 enzymes: SgfI *
October 20, 2009 10:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3480 5586 6613 278
Size: 53 83 186 936 1944 2106 1027 2468
Fragments arranged by size:
2468 2106 1944 1027 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6146 1937
Size: 4209 4594
Acc65I G'GTAC_C
Cuts at: 3537 6124 3537
Size: 2587 6216
AclI AA'CG_TT
Cuts at: 1563 6930 7303 1563
Size: 5367 373 3063
Fragments arranged by size:
5367 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2162 3102 3737 3813 4140 4927 6866 8250
Size: 530 940 635 76 327 787 1939 1384
Cuts at: 8250 8518 1632
Size: 268 1917
Fragments arranged by size:
1939 1917 1384 940 787 635 530 327
268 76
AfeI AGC'GCT
Cuts at: 4948 4948
Size: 8803
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8606
AflIII A'CryG_T
Cuts at: 1203 2657 7702 8679 1203
Size: 1454 5045 977 1327
Fragments arranged by size:
5045 1454 1327 977
AgeI A'CCGG_T
Cuts at: 3534 7634 3534
Size: 4100 4703
AhdI GACnn_n'nnGTC
Cuts at: 5587 7532 5587
Size: 1945 6858
AleI CACnn'nnGTG
Cuts at: 1166 3767 1166
Size: 2601 6202
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8803
AlwNI CAG_nnn'CTG
Cuts at: 2140 3295 4760 6546 8118 2140
Size: 1155 1465 1786 1572 2825
Fragments arranged by size:
2825 1786 1572 1465 1155
ApaLI G'TGCA_C
Cuts at: 2674 6860 8016 2674
Size: 4186 1156 3461
Fragments arranged by size:
4186 3461 1156
ApoI r'AATT_y
Cuts at: 1493 3378 4174 6112 6338 6490 8599 1493
Size: 1885 796 1938 226 152 2109 1697
Fragments arranged by size:
2109 1938 1885 1697 796 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7357 160
Size: 872 6325 1606
Fragments arranged by size:
6325 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8803
AvaI C'yCGr_G
Cuts at: 1870 1943 2282 2415 2702 2929 4696 4986 5864
Size: 73 339 133 287 227 1767 290 878
Cuts at: 5864 6128 1870
Size: 264 4545
Fragments arranged by size:
4545 1767 878 339 290 287 264 227
133 73
AvrII C'CTAG_G
Cuts at: 3229 3229
Size: 8803
BamHI G'GATC_C
Cuts at: 6133 6500 6133
Size: 367 8436
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3414 3531 3537 4031 6124
Size: 325 217 627 1627 117 6 494 2093
Cuts at: 6124 7579 618
Size: 1455 1842
Fragments arranged by size:
2093 1842 1627 1455 627 494 325 217
117 6
BanII G_rGCy'C
Cuts at: 1391 2052 3144 3696 6122 1391
Size: 661 1092 552 2426 4072
Fragments arranged by size:
4072 2426 1092 661 552
BbeI G_GCGC'C
Cuts at: 3418 3418
Size: 8803
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2591 4864 5557 6084 928
Size: 971 692 2273 693 527 3647
Fragments arranged by size:
3647 2273 971 693 692 527
BbvCI CC'TCA_GC
Cuts at: 2861 2861
Size: 8803
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2149 2183 5648 5682 6994 7028 1903
Size: 34 212 34 3465 34 1312 34 3678
Fragments arranged by size:
3678 3465 1312 212 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6695 7905 6695
Size: 1210 7593
BclI T'GATC_A
Cuts at: 1548 4809 1548
Size: 3261 5542
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2931 5624 7414
Size: 107 122 71 631 605 1259 2693 1790
Cuts at: 7414 136
Size: 1525
Fragments arranged by size:
2693 1790 1525 1259 631 605 122 107
71
BglII A'GATC_T
Cuts at: 3804 8798 3804
Size: 4994 3809
BlpI GC'TnA_GC
Cuts at: 6220 6220
Size: 8803
Bme1580I G_kGCm'C
Cuts at: 1790 2651 2678 2927 4203 6864 8020 1790
Size: 861 27 249 1276 2661 1156 2573
Fragments arranged by size:
2661 2573 1276 1156 861 249 27
BmgBI CAC'GTC
Cuts at: 1318 3504 3664 3688 1318
Size: 2186 160 24 6433
Fragments arranged by size:
6433 2186 160 24
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3313 6082 6534
Size: 416 53 83 399 24 1896 2769 452
Cuts at: 6534 7492 442
Size: 958 1753
Fragments arranged by size:
2769 1896 1753 958 452 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8803
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2944 3478 2944
Size: 534 8269
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2171 3146 4337 5801 5906 7463 1239
Size: 932 975 1191 1464 105 1557 2579
Fragments arranged by size:
2579 1557 1464 1191 975 932 105
Bpu10I CC'TnA_GC
Cuts at: 2861 5327 2861
Size: 2466 6337
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2363 5537 6928 7793 8091 2363
Size: 3174 1391 865 298 3075
Fragments arranged by size:
3174 3075 1391 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2289 3199 5283 5397 5836 7466 882
Size: 617 790 910 2084 114 439 1630 2219
Fragments arranged by size:
2219 2084 1630 910 790 617 439 114
BsaAI yAC'GTr
Cuts at: 493 1204 2658 3166 6594 493
Size: 711 1454 508 3428 2702
Fragments arranged by size:
3428 2702 1454 711 508
BsaBI GATnn'nnATC
Cuts at: 8797 8797
Size: 8803
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3415 3477 5583 6610
Size: 53 83 186 936 1882 62 2106 1027
Cuts at: 6610 6992 7627 275
Size: 382 635 1451
Fragments arranged by size:
2106 1882 1451 1027 936 635 382 186
83 62 53
BsaWI w'CCGG_w
Cuts at: 2018 3534 5274 5499 7236 7634 7908 8055 8466
Size: 1516 1740 225 1737 398 274 147 411
Cuts at: 8466 2018
Size: 2355
Fragments arranged by size:
2355 1740 1737 1516 411 398 274 225
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4310 4340 4839 4869 1178
Size: 30 165 30 2907 30 499 30 5112
Fragments arranged by size:
5112 2907 499 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2037 2579 3251 3862 4061 4160 5819 5864
Size: 851 542 672 611 199 99 1659 45
Cuts at: 5864 5888 5920 1186
Size: 24 32 4069
Fragments arranged by size:
4069 1659 851 672 611 542 199 99
45 32 24
BseYI C'CCAG_C
Cuts at: 4441 4900 5197 8006 8502 4441
Size: 459 297 2809 496 4742
Fragments arranged by size:
4742 2809 496 459 297
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2143 4166 1163
Size: 573 407 2023 5800
Fragments arranged by size:
5800 2023 573 407
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4281 6253 7014 8042 1540
Size: 275 186 2280 1972 761 1028 2301
Fragments arranged by size:
2301 2280 1972 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2678 6864 6949 8020 2678
Size: 4186 85 1071 3461
Fragments arranged by size:
4186 3461 1071 85
BsmI GAATG_Cn'
Cuts at: 6314 6407 6314
Size: 93 8710
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2052 2651 2678 2927 3144 3696 4203
Size: 399 262 599 27 249 217 552 507
Cuts at: 4203 6122 6864 6949 8020 1391
Size: 1919 742 85 1071 2174
Fragments arranged by size:
2174 1919 1071 742 599 552 507 399
262 249 217 85 27
BspEI T'CCGG_A
Cuts at: 2018 8466 2018
Size: 6448 2355
BspHI T'CATG_A
Cuts at: 6692 8422 6692
Size: 1730 7073
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 6107 6158 844
Size: 5263 51 3489
Fragments arranged by size:
5263 3489 51
BsrBI CCG'CTC
Cuts at: 1394 1823 6070 6690 1394
Size: 429 4247 620 3507
Fragments arranged by size:
4247 3507 620 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3111 7298 7472 65
Size: 1183 1863 4187 174 1396
Fragments arranged by size:
4187 1863 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2774 3534 3540 3978 4686 7447 7634
Size: 110 964 760 6 438 708 2761 187
Cuts at: 7634 1700
Size: 2869
Fragments arranged by size:
2869 2761 964 760 708 438 187 110
6
BsrGI T'GTAC_A
Cuts at: 96 3810 96
Size: 3714 5089
BssHII G'CGCG_C
Cuts at: 1925 2724 1925
Size: 799 8004
BssSI C'ACGA_G
Cuts at: 4427 6863 7875 4427
Size: 2436 1012 5355
Fragments arranged by size:
5355 2436 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2964 794
Size: 2170 6633
BstBI TT'CG_AA
Cuts at: 7607 7607
Size: 8803
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8803
BstXI CCAn_nnnn'nTGG
Cuts at: 3788 3847 4521 5062 3788
Size: 59 674 541 7529
Fragments arranged by size:
7529 674 541 59
BstYI r'GATC_y
Cuts at: 1972 3171 3559 3804 4756 6133 6500 6885 6902
Size: 1199 388 245 952 1377 367 385 17
Cuts at: 6902 8354 8440 8452 8798 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1377 1199 952 388 385 367
346 245 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2402 3960 2402
Size: 1558 7245
BtgI C'CryG_G
Cuts at: 513 1065 2323 2559 3765 3787 4275 513
Size: 552 1258 236 1206 22 488 5041
Fragments arranged by size:
5041 1258 1206 552 488 236 22
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4798 487
Size: 683 56 3572 4492
Fragments arranged by size:
4492 3572 683 56
BtsI GCAGTG_nn'
Cuts at: 5945 6319 7113 7133 5945
Size: 374 794 20 7615
Fragments arranged by size:
7615 794 374 20
ClaI AT'CG_AT
Cuts at: 1554 4407 1554
Size: 2853 5950
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8768
DraI TTT'AAA
Cuts at: 4554 6107 4554
Size: 1553 7250
DraIII CAC_nnn'GTG
Cuts at: 5185 5185
Size: 8803
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7810 809
Size: 507 6494 1802
Fragments arranged by size:
6494 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2081 4239 4278
Size: 54 1425 50 275 18 251 2158 39
Cuts at: 4278 4559 5091 5346 5625 6250 7139 8768 8
Size: 281 532 255 279 625 889 1629 43
Fragments arranged by size:
2158 1629 1425 889 625 532 281 279
275 255 251 54 50 43 39 18
EagI C'GGCC_G
Cuts at: 1537 1812 4278 6250 1537
Size: 275 2466 1972 4090
Fragments arranged by size:
4090 2466 1972 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2306 2342 3880 4305 6733 1353
Size: 953 36 1538 425 2428 3423
Fragments arranged by size:
3423 2428 1538 953 425 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2053 3164 7390 7764 7910 387
Size: 1064 138 464 1111 4226 374 146 1280
Fragments arranged by size:
4226 1280 1111 1064 464 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2162 2171 3102 3146 3737 3813 4140
Size: 393 530 9 931 44 591 76 327
Cuts at: 4140 4337 4927 5801 5906 6866 7463 8250 8518
Size: 197 590 874 105 960 597 787 268
Cuts at: 8518 1239
Size: 1524
Fragments arranged by size:
1524 960 931 874 787 597 591 590
530 393 327 268 197 105 76 44
9
EcoNI CCTnn'n_nnAGG
Cuts at: 2976 3233 2976
Size: 257 8546
EcoO109I rG'GnC_Cy
Cuts at: 2028 2542 2981 4094 2028
Size: 514 439 1113 6737
Fragments arranged by size:
6737 1113 514 439
EcoRI G'AATT_C
Cuts at: 6112 6490 6112
Size: 378 8425
EcoRV GAT'ATC
Cuts at: 4411 5479 4411
Size: 1068 7735
FspI TGC'GCA
Cuts at: 5738 7309 5738
Size: 1571 7232
FspAI rTGC'GCAy
Cuts at: 5738 5738
Size: 8803
HaeII r_GCGC'y
Cuts at: 1678 2149 2830 2868 3418 4455 4950 7950 1678
Size: 471 681 38 550 1037 495 3000 2531
Fragments arranged by size:
3000 2531 1037 681 550 495 471 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2359 2391 2480 2512 3051
Size: 32 135 32 787 32 89 32 539
Cuts at: 3051 3083 4801 4833 5573 5605 5945 5977 7444
Size: 32 1718 32 740 32 340 32 1467
Cuts at: 7444 7476 7518 7550 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1718 1467 787 740 539 340 135
89 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 6147 6393 669
Size: 988 281 4209 246 3079
Fragments arranged by size:
4209 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 5151 748
Size: 4403 4400
HpaI GTT'AAC
Cuts at: 6393 6393
Size: 8803
KasI G'GCGC_C
Cuts at: 3414 3414
Size: 8803
KpnI G_GTAC'C
Cuts at: 3541 6128 3541
Size: 2587 6216
MfeI C'AATT_G
Cuts at: 5173 6402 5173
Size: 1229 7574
MluI A'CGCG_T
Cuts at: 8679 8679
Size: 8803
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3121 3666 4482 5676 7917 8101 1305
Size: 1816 545 816 1194 2241 184 2007
Fragments arranged by size:
2241 2007 1816 1194 816 545 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4241 5093 10
Size: 54 1425 343 2409 852 3720
Fragments arranged by size:
3720 2409 1425 852 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3149 3183 3767 3786 4196 4235 4250
Size: 648 1983 34 584 19 410 39 15
Cuts at: 4250 6761 7120 7279 518
Size: 2511 359 159 2042
Fragments arranged by size:
2511 2042 1983 648 584 410 359 159
39 34 19 15
MspA1I CmG'CkG
Cuts at: 1606 2140 2325 2616 2634 2718 3648 3952 4419
Size: 534 185 291 18 84 930 304 467
Cuts at: 4419 6082 6219 6896 8044 8289 1606
Size: 1663 137 677 1148 245 2120
Fragments arranged by size:
2120 1663 1148 930 677 534 467 304
291 245 185 137 84 18
NarI GG'CG_CC
Cuts at: 3415 3415
Size: 8803
NcoI C'CATG_G
Cuts at: 513 1065 2559 3765 513
Size: 552 1494 1206 5551
Fragments arranged by size:
5551 1494 1206 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8803
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8803
NotI GC'GGCC_GC
Cuts at: 6250 6250
Size: 8803
NspI r_CATG'y
Cuts at: 1132 1229 2917 2962 5125 5722 5743 6161 7706
Size: 97 1688 45 2163 597 21 418 1545
Cuts at: 7706 1132
Size: 2229
Fragments arranged by size:
2229 2163 1688 1545 597 418 97 45
21
PasI CC'CwG_GG
Cuts at: 1348 5993 1348
Size: 4645 4158
PciI A'CATG_T
Cuts at: 7702 7702
Size: 8803
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3648 1108
Size: 2540 6263
PfoI T'CCnGG_A
Cuts at: 2484 5778 5823 2484
Size: 3294 45 5464
Fragments arranged by size:
5464 3294 45
PmeI GTTT'AAAC
Cuts at: 6107 6107
Size: 8803
PmlI CAC'GTG
Cuts at: 2658 2658
Size: 8803
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3890 3922 6872 6904 8385 8417 3890
Size: 32 2950 32 1481 32 4276
Fragments arranged by size:
4276 2950 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2028 2542 2981 2028
Size: 514 439 7850
Fragments arranged by size:
7850 514 439
PshAI GACnn'nnGTC
Cuts at: 1613 2547 5709 1613
Size: 934 3162 4707
Fragments arranged by size:
4707 3162 934
PsiI TTA'TAA
Cuts at: 6373 6373
Size: 8803
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4922 4954 4922
Size: 32 8771
PstI C_TGCA'G
Cuts at: 830 2723 4451 6155 830
Size: 1893 1728 1704 3478
Fragments arranged by size:
3478 1893 1728 1704
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8803
PvuII CAG'CTG
Cuts at: 2140 2718 3648 4419 2140
Size: 578 930 771 6524
Fragments arranged by size:
6524 930 771 578
SacII CC_GC'GG
Cuts at: 2326 2326
Size: 8803
SalI G'TCGA_C
Cuts at: 1936 6145 1936
Size: 4209 4594
SanDI GG'GwC_CC
Cuts at: 2028 2028
Size: 8803
SapI GCTCTTCn'nnn_
Cuts at: 4305 4305
Size: 8803
SbfI CC_TGCA'GG
Cuts at: 6155 6155
Size: 8803
ScaI AGT'ACT
Cuts at: 2255 4940 7051 8763 2255
Size: 2685 2111 1712 2295
Fragments arranged by size:
2685 2295 2111 1712
SfcI C'TryA_G
Cuts at: 826 1045 2719 2809 3553 4447 5842 6151 7286
Size: 219 1674 90 744 894 1395 309 1135
Cuts at: 7286 7967 8158 826
Size: 681 191 1471
Fragments arranged by size:
1674 1471 1395 1135 894 744 681 309
219 191 90
SfoI GGC'GCC
Cuts at: 3416 3416
Size: 8803
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8803
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8803
SmaI CCC'GGG
Cuts at: 1872 4698 6130 1872
Size: 2826 1432 4545
Fragments arranged by size:
4545 2826 1432
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2282 2378 5552 6907 7808 8070
Size: 197 926 339 96 3174 1355 901 262
Cuts at: 8070 820
Size: 1553
Fragments arranged by size:
3174 1553 1355 926 901 339 262 197
96
SnaBI TAC'GTA
Cuts at: 493 6594 493
Size: 6101 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8803
SphI G_CATG'C
Cuts at: 1132 2917 5125 6161 1132
Size: 1785 2208 1036 3774
Fragments arranged by size:
3774 2208 1785 1036
SspI AAT'ATT
Cuts at: 5 52 6727 5
Size: 47 6675 2081
Fragments arranged by size:
6675 2081 47
StuI AGG'CCT
Cuts at: 1517 4534 5770 1517
Size: 3017 1236 4550
Fragments arranged by size:
4550 3017 1236
StyI C'CwwG_G
Cuts at: 513 1065 2199 2559 3055 3189 3229 3765 3846
Size: 552 1134 360 496 134 40 536 81
Cuts at: 3846 4529 5055 5212 6242 513
Size: 683 526 157 1030 3074
Fragments arranged by size:
3074 1134 1030 683 552 536 526 496
360 157 134 81 40
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5189 6830 6847 7000 7185 7630 325
Size: 1255 3609 1641 17 153 185 445 1498
Fragments arranged by size:
3609 1641 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2253 2604 2961
Size: 274 80 33 51 1443 276 351 357
Cuts at: 2961 3342 3810 4938 7049 8761 96
Size: 381 468 1128 2111 1712 138
Fragments arranged by size:
2111 1712 1443 1128 468 381 357 351
276 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4839 4871 8763 8795 4839
Size: 32 3892 32 4847
Fragments arranged by size:
4847 3892 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8803
XbaI T'CTAG_A
Cuts at: 6139 6139
Size: 8803
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3685 3685
Size: 8803
XhoI C'TCGA_G
Cuts at: 1943 2282 1943
Size: 339 8464
XmaI C'CCGG_G
Cuts at: 1870 4696 6128 1870
Size: 2826 1432 4545
Fragments arranged by size:
4545 2826 1432
XmnI GAAnn'nnTTC
Cuts at: 2336 5168 6932 2336
Size: 2832 1764 4207
Fragments arranged by size:
4207 2832 1764
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3478 5584 6611 276
Size: 53 83 186 936 1944 2106 1027 2468
Fragments arranged by size:
2468 2106 1944 1027 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FspI FspAI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacII
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI BaeI BfrBI BsiWI BsmBI
BstZ17I EcoICRI FalI FseI NaeI NgoMIV NruI NsiI
PacI PspOMI PspXI RsrII SacI SexAI SfiI SrfI
SwaI