Restriction map for
pFN21AB7024
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# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvrII BalI BamHI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaI BsaWI BseAI BsiWI
BspDI BspEI BspHI BspLU11I BspMI
BsrDI Bst1107I BstBI BstXI BstZ17I
CelII ClaI CpoI DraI DraII
DrdI Eam1105I EarI Eco47III EcoO109I
EcoRI EcoT14I EcoT22I FbaI FseI
HincII HindII HindIII HpaI KpnI
Ksp632I MaeII MamI MfeI MluI
MluNI MroI MscI MseI MunI
NcoI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SalI SapI
SbfI ScaI SexAI SfiI SfuI
SnaBI SpeI SphI Sse8387I SspI
StuI StyI SwaI Tru9I Tth111I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AocI 871 CCTCAGG CC^T[ACGT]AGG
ApaI 408 GGGCCC GGGCC^C
AspHI 295 GTGCTC G[AT]GC[AT]^C
AvaII 8 GGACC G^G[AT]CC
AviII 438 TGCGCA TGC^GCA
BbsI 807 GAAGAC GAAGAC(2/6)
BglI 244 GCCGGCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 807 GAAGAC GAAGAC(2/6)
BsiHKAI 295 GTGCTC G[AT]GC[AT]^C
BsmBI 34 CGTCTC CGTCTC(1/5)
BsmI 507 GCATTC GAATGC(1/-1)
Bsp1407I 995 TGTACA T^GTACA
BsrBI 313 GAGCGG CCGCTC(-3/-3)
BsrGI 995 TGTACA T^GTACA
BssHII 428 GCGCGC G^CGCGC
BssSI 1147 CACGAG CACGAG(-5/-1)
BstEII 211 GGTCACC G^GT[ACGT]ACC
BstPI 211 GGTCACC G^GT[ACGT]ACC
Bsu36I 871 CCTCAGG CC^T[ACGT]AGG
Eco81I 871 CCTCAGG CC^T[ACGT]AGG
EcoNI 183 CCTGGGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 211 GGTCACC G^GT[ACGT]ACC
EcoRV 853 GATATC GAT^ATC
FspI 438 TGCGCA TGC^GCA
MslI 776 CACACTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NlaIII 142 CATG CATG^
PflMI 336 CCAGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SmlI 298 CTCAAG C^T[CT][GA]AG
SspBI 995 TGTACA T^GTACA
TaqI 1106 TCGA T^CGA
TthHB8I 1106 TCGA T^CGA
Van91I 336 CCAGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AlwNI 677 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
1236 CAGCAGCTG
BanII 408 GGGCCC G[GA]GC[CT]^C
841 GAGCCC
BfaI 610 CTAG C^TAG
1255 CTAG
BsgI 928 GTGCAG GTGCAG(16/14)
1183 GTGCAG
BsiEI 251 CGGCCG CG[GA][CT]^CG
387 CGGCCG
BsmAI 35 GTCTC GTCTC(1/5)
615 GTCTC
BsmFI 358 GGGAC GGGAC(10/14)
348 GTCCC
BstYI 270 GGATCT [GA]^GATC[CT]
498 GGATCT
EagI 251 CGGCCG C^GGCCG
387 CGGCCG
EclXI 251 CGGCCG C^GGCCG
387 CGGCCG
Eco52I 251 CGGCCG C^GGCCG
387 CGGCCG
HphI 213 TCACC GGTGA(8/7)
588 TCACC
KspI 236 CCGCGG CCGC^GG
351 CCGCGG
MaeI 610 CTAG C^TAG
1255 CTAG
MflI 270 GGATCT [GA]^GATC[CT]
498 GGATCT
NaeI 244 GCCGGC GCC^GGC
424 GCCGGC
NgoMIV 244 GCCGGC G^CCGGC
424 GCCGGC
SacII 236 CCGCGG CCGC^GG
351 CCGCGG
SgrAI 243 CGCCGGCG C[GA]^CCGG[CT]G
423 CGCCGGCG
SmaI 162 CCCGGG CCC^GGG
411 CCCGGG
TfiI 541 GAATC G^A[AT]TC
742 GATTC
Tsp45I 212 GTCAC ^GT[GC]AC
774 GTCAC
Tsp509I 715 AATT ^AATT
904 AATT
XcmI 635 CCAGATACCCGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1055 CCAGGACGCTGTTGG
XhoII 270 GGATCT [GA]^GATC[CT]
498 GGATCT
XmaCI 162 CCCGGG C^CCGGG
411 CCCGGG
XmaI 162 CCCGGG C^CCGGG
411 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AvaI BanI BbeI BbvI
BcnI BmyI BpmI BsaHI BsaJI
BseRI BsiYI BslI BsoBI Bsp1286I
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraIII DsaI
EaeI EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII ItaI
KasI MaeIII MboI MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NdeII NlaIV PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI TseI
TspRI