(Circular) (Six-base) MAPSORT of: pFN21AB7024.seq Check: 3054 from: 1 to: 5983 pFN21AB7024 5983 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3793 278 Size: 53 83 186 936 2257 2468 Fragments arranged by size: 2468 2257 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3326 1937 Size: 1389 4594 Acc65I G'GTAC_C Cuts at: 3304 3304 Size: 5983 AclI AA'CG_TT Cuts at: 1563 4110 4483 1563 Size: 2547 373 3063 Fragments arranged by size: 3063 2547 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2300 2333 4046 5430 5698 1632 Size: 668 33 1713 1384 268 1917 Fragments arranged by size: 1917 1713 1384 668 268 33 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5786 AflIII A'CryG_T Cuts at: 1203 4882 5859 1203 Size: 3679 977 1327 Fragments arranged by size: 3679 1327 977 AgeI A'CCGG_T Cuts at: 4814 4814 Size: 5983 AhdI GACnn_n'nnGTC Cuts at: 4712 4712 Size: 5983 AleI CACnn'nnGTG Cuts at: 1166 2785 1166 Size: 1619 4364 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2216 1184 Size: 1032 4951 AlwNI CAG_nnn'CTG Cuts at: 2687 3246 3726 5298 2687 Size: 559 480 1572 3372 Fragments arranged by size: 3372 1572 559 480 ApaI G_GGCC'C Cuts at: 2417 2417 Size: 5983 ApaLI G'TGCA_C Cuts at: 4040 5196 4040 Size: 1156 4827 ApoI r'AATT_y Cuts at: 1493 3292 3518 3670 5779 1493 Size: 1799 226 152 2109 1697 Fragments arranged by size: 2109 1799 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4537 160 Size: 872 3505 1606 Fragments arranged by size: 3505 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5983 AvaI C'yCGr_G Cuts at: 1870 1943 2167 2416 2567 3308 1870 Size: 73 224 249 151 741 4545 Fragments arranged by size: 4545 741 249 224 151 73 BamHI G'GATC_C Cuts at: 3313 3680 3313 Size: 367 5616 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2207 2246 2264 2422 3304 Size: 325 217 627 420 39 18 158 882 Cuts at: 3304 4759 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 882 627 420 325 217 158 39 18 BanII G_rGCy'C Cuts at: 1391 2417 2850 3302 1391 Size: 1026 433 452 4072 Fragments arranged by size: 4072 1026 452 433 BbeI G_GCGC'C Cuts at: 2250 2268 2426 2250 Size: 18 158 5807 Fragments arranged by size: 5807 158 18 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2819 928 Size: 971 920 4092 Fragments arranged by size: 4092 971 920 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4174 4208 1903 Size: 34 2237 34 3678 Fragments arranged by size: 3678 2237 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3875 5085 3875 Size: 1210 4773 BclI T'GATC_A Cuts at: 1548 1548 Size: 5983 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2255 4594 136 Size: 107 122 71 631 605 583 2339 1525 Fragments arranged by size: 2339 1525 631 605 583 122 107 71 BglII A'GATC_T Cuts at: 5978 5978 Size: 5983 BlpI GC'TnA_GC Cuts at: 3400 3400 Size: 5983 Bme1580I G_kGCm'C Cuts at: 1790 2417 4044 5200 1790 Size: 627 1627 1156 2573 Fragments arranged by size: 2573 1627 1156 627 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5983 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2812 3714 4672 Size: 416 53 83 399 24 1395 902 958 Cuts at: 4672 442 Size: 1753 Fragments arranged by size: 1753 1395 958 902 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5983 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2065 2324 2348 2623 2783 3043 4643 1239 Size: 826 259 24 275 160 260 1600 2579 Fragments arranged by size: 2579 1600 826 275 260 259 160 24 Bpu10I CC'TnA_GC Cuts at: 2597 2597 Size: 5983 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2288 4108 4973 5271 2288 Size: 1820 865 298 3000 Fragments arranged by size: 3000 1820 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4646 882 Size: 617 3147 2219 Fragments arranged by size: 3147 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3774 493 Size: 711 2570 2702 Fragments arranged by size: 2702 2570 711 BsaBI GATnn'nnATC Cuts at: 5977 5977 Size: 5983 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2038 2247 2265 2423 Size: 53 83 186 936 505 209 18 158 Cuts at: 2423 3790 4172 4807 275 Size: 1367 382 635 1451 Fragments arranged by size: 1451 1367 936 635 505 382 209 186 158 83 53 18 BsaWI w'CCGG_w Cuts at: 4416 4814 5088 5235 5646 4416 Size: 398 274 147 411 4753 Fragments arranged by size: 4753 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2038 2068 3041 3071 1178 Size: 30 165 30 635 30 973 30 4090 Fragments arranged by size: 4090 973 635 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2062 2090 2093 2115 2118 2822 1186 Size: 876 28 3 22 3 704 4347 Fragments arranged by size: 4347 876 704 28 22 3 3 BseYI C'CCAG_C Cuts at: 2410 3210 5186 5682 2410 Size: 800 1976 496 2711 Fragments arranged by size: 2711 1976 800 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2954 3209 1163 Size: 573 1218 255 3937 Fragments arranged by size: 3937 1218 573 255 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2259 2395 3433 4194 5222 1540 Size: 275 186 258 136 1038 761 1028 2301 Fragments arranged by size: 2301 1038 1028 761 275 258 186 136 BsiHKAI G_wGCw'C Cuts at: 2304 4044 4129 5200 2304 Size: 1740 85 1071 3087 Fragments arranged by size: 3087 1740 1071 85 BsmI GAATG_Cn' Cuts at: 2512 3494 3587 2512 Size: 982 93 4908 Fragments arranged by size: 4908 982 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2045 2045 Size: 5983 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2304 2417 2850 3302 4044 4129 5200 Size: 399 514 113 433 452 742 85 1071 Cuts at: 5200 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 514 452 433 399 113 85 BspEI T'CCGG_A Cuts at: 5646 5646 Size: 5983 BspHI T'CATG_A Cuts at: 3872 5602 3872 Size: 1730 4253 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3338 844 Size: 2494 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2320 3870 1394 Size: 429 497 1550 3507 Fragments arranged by size: 3507 1550 497 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4478 4652 65 Size: 1183 3230 174 1396 Fragments arranged by size: 3230 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2153 2249 2429 4627 4814 1700 Size: 110 343 96 180 2198 187 2869 Fragments arranged by size: 2869 2198 343 187 180 110 96 BsrGI T'GTAC_A Cuts at: 96 3000 96 Size: 2904 3079 BssHII G'CGCG_C Cuts at: 1925 2433 1925 Size: 508 5475 BssSI C'ACGA_G Cuts at: 3152 4043 5055 3152 Size: 891 1012 4080 Fragments arranged by size: 4080 1012 891 BstAPI GCAn_nnn'nTGC Cuts at: 794 2299 2376 2941 794 Size: 1505 77 565 3836 Fragments arranged by size: 3836 1505 565 77 BstBI TT'CG_AA Cuts at: 4787 4787 Size: 5983 BstEII G'GTnAC_C Cuts at: 1184 2216 1184 Size: 1032 4951 BstYI r'GATC_y Cuts at: 1972 2275 2503 3313 3680 4065 4082 5534 5620 Size: 303 228 810 367 385 17 1452 86 Cuts at: 5620 5632 5978 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 810 385 367 346 303 228 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2877 2877 Size: 5983 BtgI C'CryG_G Cuts at: 513 1065 2241 2346 2356 3170 513 Size: 552 1176 105 10 814 3326 Fragments arranged by size: 3326 1176 814 552 105 10 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5244 Fragments arranged by size: 5244 683 56 BtsI GCAGTG_nn' Cuts at: 3499 4293 4313 3499 Size: 794 20 5169 Fragments arranged by size: 5169 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5983 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5948 DraI TTT'AAA Cuts at: 3287 3287 Size: 5983 DraIII CAC_nnn'GTG Cuts at: 2299 2376 3082 2299 Size: 77 706 5200 Fragments arranged by size: 5200 706 77 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4990 809 Size: 507 3674 1802 Fragments arranged by size: 3674 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2256 2392 2498 Size: 54 1425 50 275 18 426 136 106 Cuts at: 2498 3430 4319 5948 8 Size: 932 889 1629 43 Fragments arranged by size: 1629 1425 932 889 426 275 136 106 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2256 2392 3430 1537 Size: 275 444 136 1038 4090 Fragments arranged by size: 4090 1038 444 275 136 EarI CTCTTCn'nnn_ Cuts at: 1353 3913 1353 Size: 2560 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2288 3025 4570 4944 5090 387 Size: 1064 138 699 737 1545 374 146 1280 Fragments arranged by size: 1545 1280 1064 737 699 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2065 2300 2324 2333 2348 2623 2783 Size: 393 433 235 24 9 15 275 160 Cuts at: 2783 3043 4046 4643 5430 5698 1239 Size: 260 1003 597 787 268 1524 Fragments arranged by size: 1524 1003 787 597 433 393 275 268 260 235 160 24 15 9 EcoNI CCTnn'n_nnAGG Cuts at: 2192 2192 Size: 5983 EcoRI G'AATT_C Cuts at: 3292 3670 3292 Size: 378 5605 EcoRV GAT'ATC Cuts at: 2860 2860 Size: 5983 FspI TGC'GCA Cuts at: 2445 4489 2445 Size: 2044 3939 HaeII r_GCGC'y Cuts at: 1678 2250 2268 2288 2426 5130 1678 Size: 572 18 20 138 2704 2531 Fragments arranged by size: 2704 2531 572 138 20 18 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2013 2045 4624 4656 4698 Size: 32 135 32 441 32 2579 32 42 Cuts at: 4698 4730 1373 Size: 32 2626 Fragments arranged by size: 2626 2579 441 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3327 3573 669 Size: 988 281 1389 246 3079 Fragments arranged by size: 3079 1389 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5983 HpaI GTT'AAC Cuts at: 3573 3573 Size: 5983 KasI G'GCGC_C Cuts at: 2246 2264 2422 2246 Size: 18 158 5807 Fragments arranged by size: 5807 158 18 KpnI G_GTAC'C Cuts at: 3308 3308 Size: 5983 MfeI C'AATT_G Cuts at: 3582 3582 Size: 5983 MluI A'CGCG_T Cuts at: 5859 5859 Size: 5983 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5097 5281 1305 Size: 3792 184 2007 Fragments arranged by size: 3792 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4161 Fragments arranged by size: 4161 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2785 3941 4300 4459 518 Size: 648 1619 1156 359 159 2042 Fragments arranged by size: 2042 1619 1156 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2243 2326 2358 2702 2929 2971 3139 3246 Size: 637 83 32 344 227 42 168 107 Cuts at: 3246 3399 4076 5224 5469 1606 Size: 153 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 637 344 245 227 168 153 107 83 42 32 NaeI GCC'GGC Cuts at: 2251 2431 2251 Size: 180 5803 NarI GG'CG_CC Cuts at: 2247 2265 2423 2247 Size: 18 158 5807 Fragments arranged by size: 5807 158 18 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5431 NdeI CA'TA_TG Cuts at: 387 387 Size: 5983 NgoMIV G'CCGG_C Cuts at: 2249 2429 2249 Size: 180 5803 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5983 NotI GC'GGCC_GC Cuts at: 3430 3430 Size: 5983 NspI r_CATG'y Cuts at: 1132 1229 3341 4886 1132 Size: 97 2112 1545 2229 Fragments arranged by size: 2229 2112 1545 97 PasI CC'CwG_GG Cuts at: 1348 2732 1348 Size: 1384 4599 PciI A'CATG_T Cuts at: 4882 4882 Size: 5983 PflMI CCAn_nnn'nTGG Cuts at: 1108 2347 1108 Size: 1239 4744 PfoI T'CCnGG_A Cuts at: 2841 3059 2841 Size: 218 5765 PmeI GTTT'AAAC Cuts at: 3287 3287 Size: 5983 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4052 4084 5565 5597 4052 Size: 32 1481 32 4438 Fragments arranged by size: 4438 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5983 PsiI TTA'TAA Cuts at: 3553 3553 Size: 5983 PspOMI G'GGCC_C Cuts at: 2413 2413 Size: 5983 PstI C_TGCA'G Cuts at: 830 2408 2674 3138 3335 830 Size: 1578 266 464 197 3478 Fragments arranged by size: 3478 1578 464 266 197 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5983 PvuII CAG'CTG Cuts at: 2326 2929 2971 3246 2326 Size: 603 42 275 5063 Fragments arranged by size: 5063 603 275 42 SacII CC_GC'GG Cuts at: 2244 2359 2244 Size: 115 5868 SalI G'TCGA_C Cuts at: 1936 3325 1936 Size: 1389 4594 SbfI CC_TGCA'GG Cuts at: 3335 3335 Size: 5983 ScaI AGT'ACT Cuts at: 4231 5943 4231 Size: 1712 4271 SfcI C'TryA_G Cuts at: 826 1045 2404 2670 3134 3331 4466 5147 5338 Size: 219 1359 266 464 197 1135 681 191 Cuts at: 5338 826 Size: 1471 Fragments arranged by size: 1471 1359 1135 681 464 266 219 197 191 SfoI GGC'GCC Cuts at: 2248 2266 2424 2248 Size: 18 158 5807 Fragments arranged by size: 5807 158 18 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5983 SgrAI Cr'CCGG_yG Cuts at: 1700 2249 2429 1700 Size: 549 180 5254 Fragments arranged by size: 5254 549 180 SmaI CCC'GGG Cuts at: 1872 2169 2418 3310 1872 Size: 297 249 892 4545 Fragments arranged by size: 4545 892 297 249 SmlI C'TyrA_G Cuts at: 820 1017 1943 2303 4087 4988 5250 820 Size: 197 926 360 1784 901 262 1553 Fragments arranged by size: 1784 1553 926 901 360 262 197 SnaBI TAC'GTA Cuts at: 493 3774 493 Size: 3281 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5983 SphI G_CATG'C Cuts at: 1132 3341 1132 Size: 2209 3774 SrfI GCCC'GGGC Cuts at: 2169 2169 Size: 5983 SspI AAT'ATT Cuts at: 5 52 3907 5 Size: 47 3855 2081 Fragments arranged by size: 3855 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5983 StyI C'CwwG_G Cuts at: 513 1065 3422 513 Size: 552 2357 3074 Fragments arranged by size: 3074 2357 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2766 2986 4010 4027 4180 4365 4810 Size: 1255 1186 220 1024 17 153 185 445 Cuts at: 4810 325 Size: 1498 Fragments arranged by size: 1498 1255 1186 1024 445 220 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3000 4229 5941 Size: 274 80 33 51 1443 1023 1229 1712 Cuts at: 5941 96 Size: 138 Fragments arranged by size: 1712 1443 1229 1023 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2038 2070 2978 3010 5943 5975 2038 Size: 32 908 32 2933 32 2046 Fragments arranged by size: 2933 2046 908 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5983 XbaI T'CTAG_A Cuts at: 3319 3319 Size: 5983 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2647 3067 2647 Size: 420 5563 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5983 XmaI C'CCGG_G Cuts at: 1870 2167 2416 3308 1870 Size: 297 249 892 4545 Fragments arranged by size: 4545 892 297 249 XmnI GAAnn'nnTTC Cuts at: 4112 4112 Size: 5983 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3791 276 Size: 53 83 186 936 2257 2468 Fragments arranged by size: 2468 2257 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I EcoICRI EcoO109I FalI FseI FspAI NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SwaI