Restriction map for pFN21AB7024
 (Linear) (Six-base) MAPSORT of: KIBB7024.seq  Check: 4200  from: 1  to: 1278



KIBB7024.orf

 With 182 enzymes: SgfI * 

                        February 20, 2009 16:23  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     295     328    1278 
   Size:        295      33     950

  Fragments arranged by size: 

                 950     295      33
 
AleI CACnn'nnGTG

Cuts at:      0     780    1278 
   Size:        780     498
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     211    1278 
   Size:        211    1067
 
AlwNI CAG_nnn'CTG

Cuts at:      0     682    1241    1278 
   Size:        682     559      37

  Fragments arranged by size: 

                 682     559      37
 
ApaI G_GGCC'C

Cuts at:      0     412    1278 
   Size:        412     866
 
AvaI C'yCGr_G

Cuts at:      0     162     411     562    1278 
   Size:        162     249     151     716

  Fragments arranged by size: 

                 716     249     162     151
 
BanI G'GyrC_C

Cuts at:      0     202     241     259     417    1278 
   Size:        202      39      18     158     861

  Fragments arranged by size: 

                 861     202     158      39      18
 
BanII G_rGCy'C

Cuts at:      0     412     845    1278 
   Size:        412     433     433

  Fragments arranged by size: 

                 433     433     412
 
BbeI G_GCGC'C

Cuts at:      0     245     263     421    1278 
   Size:        245      18     158     857

  Fragments arranged by size: 

                 857     245     158      18
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     814    1278 
   Size:        814     464
 
BglI GCCn_nnn'nGGC

Cuts at:      0     250    1278 
   Size:        250    1028
 
Bme1580I G_kGCm'C

Cuts at:      0     412    1278 
   Size:        412     866
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     807    1278 
   Size:        807     471
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      60     319     343     618     778    1038    1278 
   Size:         60     259      24     275     160     260     240

  Fragments arranged by size: 

                 275     260     259     240     160      60      24
 
Bpu10I CC'TnA_GC

Cuts at:      0     592    1278 
   Size:        592     686
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     283    1278 
   Size:        283     995
 
BsaHI Gr'CG_yC

Cuts at:      0      33     242     260     418    1278 
   Size:         33     209      18     158     860

  Fragments arranged by size: 

                 860     209     158      33      18
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      33      63    1036    1066    1278 
   Size:         33      30     973      30     212

  Fragments arranged by size: 

                 973     212      33      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      57      85      88     110     113     817    1278 
   Size:         57      28       3      22       3     704     461

  Fragments arranged by size: 

                 704     461      57      28      22       3       3
 
BseYI C'CCAG_C

Cuts at:      0     405    1205    1278 
   Size:        405     800      73

  Fragments arranged by size: 

                 800     405      73
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     949    1204    1278 
   Size:        949     255      74

  Fragments arranged by size: 

                 949     255      74
 
BsiEI CG_ry'CG

Cuts at:      0     254     390    1278 
   Size:        254     136     888

  Fragments arranged by size: 

                 888     254     136
 
BsiHKAI G_wGCw'C

Cuts at:      0     299    1278 
   Size:        299     979
 
BsmI GAATG_Cn'

Cuts at:      0     507    1278 
   Size:        507     771
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      40    1278 
   Size:         40    1238
 
Bsp1286I G_dGCh'C

Cuts at:      0     299     412     845    1278 
   Size:        299     113     433     433

  Fragments arranged by size: 

                 433     433     299     113
 
BsrBI CCG'CTC

Cuts at:      0     315    1278 
   Size:        315     963
 
BsrFI r'CCGG_y

Cuts at:      0     148     244     424    1278 
   Size:        148      96     180     854

  Fragments arranged by size: 

                 854     180     148      96
 
BsrGI T'GTAC_A

Cuts at:      0     995    1278 
   Size:        995     283
 
BssHII G'CGCG_C

Cuts at:      0     428    1278 
   Size:        428     850
 
BssSI C'ACGA_G

Cuts at:      0    1147    1278 
   Size:       1147     131
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     294     371     936    1278 
   Size:        294      77     565     342

  Fragments arranged by size: 

                 565     342     294      77
 
BstEII G'GTnAC_C

Cuts at:      0     211    1278 
   Size:        211    1067
 
BstYI r'GATC_y

Cuts at:      0     270     498    1278 
   Size:        270     228     780

  Fragments arranged by size: 

                 780     270     228
 
Bsu36I CC'TnA_GG

Cuts at:      0     872    1278 
   Size:        872     406
 
BtgI C'CryG_G

Cuts at:      0     236     341     351    1165    1278 
   Size:        236     105      10     814     113

  Fragments arranged by size: 

                 814     236     113     105      10
 
DraIII CAC_nnn'GTG

Cuts at:      0     294     371    1077    1278 
   Size:        294      77     706     201

  Fragments arranged by size: 

                 706     294     201      77
 
EaeI y'GGCC_r

Cuts at:      0     251     387     493    1278 
   Size:        251     136     106     785

  Fragments arranged by size: 

                 785     251     136     106
 
EagI C'GGCC_G

Cuts at:      0     251     387    1278 
   Size:        251     136     891

  Fragments arranged by size: 

                 891     251     136
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     283    1020    1278 
   Size:        283     737     258

  Fragments arranged by size: 

                 737     283     258
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      60     295     319     328     343     618     778    1038 
   Size:         60     235      24       9      15     275     160     260

Cuts at:   1038    1278 
   Size:        240

  Fragments arranged by size: 

                 275     260     240     235     160      60      24      15
                   9
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     187    1278 
   Size:        187    1091
 
EcoRV GAT'ATC

Cuts at:      0     855    1278 
   Size:        855     423
 
FspI TGC'GCA

Cuts at:      0     440    1278 
   Size:        440     838
 
HaeII r_GCGC'y

Cuts at:      0     245     263     283     421    1278 
   Size:        245      18      20     138     857

  Fragments arranged by size: 

                 857     245     138      20      18
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0       8      40    1278 
   Size:          8      32    1238

  Fragments arranged by size: 

                1238      32       8
 
KasI G'GCGC_C

Cuts at:      0     241     259     417    1278 
   Size:        241      18     158     861

  Fragments arranged by size: 

                 861     241     158      18
 
MslI CAynn'nnrTG

Cuts at:      0     780    1278 
   Size:        780     498
 
MspA1I CmG'CkG

Cuts at:      0     238     321     353     697     924     966    1134    1241 
   Size:        238      83      32     344     227      42     168     107

Cuts at:   1241    1278 
   Size:         37

  Fragments arranged by size: 

                 344     238     227     168     107      83      42      37
                  32
 
NaeI GCC'GGC

Cuts at:      0     246     426    1278 
   Size:        246     180     852

  Fragments arranged by size: 

                 852     246     180
 
NarI GG'CG_CC

Cuts at:      0     242     260     418    1278 
   Size:        242      18     158     860

  Fragments arranged by size: 

                 860     242     158      18
 
NgoMIV G'CCGG_C

Cuts at:      0     244     424    1278 
   Size:        244     180     854

  Fragments arranged by size: 

                 854     244     180
 
PasI CC'CwG_GG

Cuts at:      0     727    1278 
   Size:        727     551
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     342    1278 
   Size:        342     936
 
PfoI T'CCnGG_A

Cuts at:      0     836    1054    1278 
   Size:        836     218     224

  Fragments arranged by size: 

                 836     224     218
 
PspOMI G'GGCC_C

Cuts at:      0     408    1278 
   Size:        408     870
 
PstI C_TGCA'G

Cuts at:      0     403     669    1133    1278 
   Size:        403     266     464     145

  Fragments arranged by size: 

                 464     403     266     145
 
PvuII CAG'CTG

Cuts at:      0     321     924     966    1241    1278 
   Size:        321     603      42     275      37

  Fragments arranged by size: 

                 603     321     275      42      37
 
SacII CC_GC'GG

Cuts at:      0     239     354    1278 
   Size:        239     115     924

  Fragments arranged by size: 

                 924     239     115
 
SfcI C'TryA_G

Cuts at:      0     399     665    1129    1278 
   Size:        399     266     464     149

  Fragments arranged by size: 

                 464     399     266     149
 
SfoI GGC'GCC

Cuts at:      0     243     261     419    1278 
   Size:        243      18     158     859

  Fragments arranged by size: 

                 859     243     158      18
 
SgrAI Cr'CCGG_yG

Cuts at:      0     244     424    1278 
   Size:        244     180     854

  Fragments arranged by size: 

                 854     244     180
 
SmaI CCC'GGG

Cuts at:      0     164     413    1278 
   Size:        164     249     865

  Fragments arranged by size: 

                 865     249     164
 
SmlI C'TyrA_G

Cuts at:      0     298    1278 
   Size:        298     980
 
SrfI GCCC'GGGC

Cuts at:      0     164    1278 
   Size:        164    1114
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     761     981    1278 
   Size:        761     220     297

  Fragments arranged by size: 

                 761     297     220
 
TatI w'GTAC_w

Cuts at:      0     995    1278 
   Size:        995     283
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      33      65     973    1005    1278 
   Size:         33      32     908      32     273

  Fragments arranged by size: 

                 908     273      33      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     642    1062    1278 
   Size:        642     420     216

  Fragments arranged by size: 

                 642     420     216
 
XmaI C'CCGG_G

Cuts at:      0     162     411    1278 
   Size:        162     249     867

  Fragments arranged by size: 

                 867     249     162
 

 Enzymes that do cut:

     AcuI     AleI     AlfI    AlwNI     ApaI     AvaI     BanI    BanII
     BbeI     BbsI     BglI Bme1580I     BmrI     BpmI   Bpu10I    BpuEI
    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
    BsmBI Bsp1286I    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
   BstEII    BstYI   Bsu36I     BtgI   DraIII     EaeI     EagI     EciI
  Eco57MI    EcoNI    EcoRV     FspI    HaeII    Hin4I     KasI     MslI
   MspA1I     NaeI     NarI   NgoMIV     PasI    PflMI     PfoI   PspOMI
     PstI    PvuII    SacII     SfcI     SfoI    SgrAI     SmaI     SmlI
     SrfI    TaqII     TatI     TstI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI    BbvCI     BcgI    BciVI     BclI    BfrBI
    BglII     BlpI    BmgBI     BmtI     BplI     BsaI    BsaAI    BsaBI
    BsaWI    BsiWI    BspEI    BspHI    BspMI    BsrDI    BstBI    BstXI
  BstZ17I    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EarI
  EcoICRI EcoO109I    EcoRI     FalI     FseI    FspAI   HincII  HindIII
     HpaI     KpnI     MfeI     MluI     MmeI     MscI     NcoI     NdeI
     NheI     NotI     NruI     NsiI     NspI     PacI     PciI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI     PvuI
    RsrII     SacI     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SgfI    SnaBI     SpeI     SphI     SspI     StuI     StyI
     SwaI  Tth111I     XbaI     XhoI     XmnI     ZraI
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