(Circular) (Six-base) MAPSORT of: pFN21AB7016.seq Check: 2941 from: 1 to: 7210 pFN21AB7016 7210 bp With 182 enzymes: SgfI * February 27, 2009 14:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2696 3969 2696 Size: 1273 5937 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5020 278 Size: 53 83 186 936 3484 2468 Fragments arranged by size: 3484 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4553 1937 Size: 2616 4594 Acc65I G'GTAC_C Cuts at: 3833 4531 3833 Size: 698 6512 AclI AA'CG_TT Cuts at: 1563 5337 5710 1563 Size: 3774 373 3063 Fragments arranged by size: 3774 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4049 5273 6657 6925 1632 Size: 2417 1224 1384 268 1917 Fragments arranged by size: 2417 1917 1384 1224 268 AfeI AGC'GCT Cuts at: 2099 4373 2099 Size: 2274 4936 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7013 AflIII A'CryG_T Cuts at: 1203 6109 7086 1203 Size: 4906 977 1327 Fragments arranged by size: 4906 1327 977 AgeI A'CCGG_T Cuts at: 6041 6041 Size: 7210 AhdI GACnn_n'nnGTC Cuts at: 4351 5939 4351 Size: 1588 5622 AleI CACnn'nnGTG Cuts at: 1166 2345 4076 1166 Size: 1179 1731 4300 Fragments arranged by size: 4300 1731 1179 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7210 AlwNI CAG_nnn'CTG Cuts at: 2499 3263 3611 4483 4953 6525 2499 Size: 764 348 872 470 1572 3184 Fragments arranged by size: 3184 1572 872 764 470 348 ApaI G_GGCC'C Cuts at: 2069 2286 3130 3297 3719 2069 Size: 217 844 167 422 5560 Fragments arranged by size: 5560 844 422 217 167 ApaLI G'TGCA_C Cuts at: 5267 6423 5267 Size: 1156 6054 ApoI r'AATT_y Cuts at: 1493 4519 4745 4897 7006 1493 Size: 3026 226 152 2109 1697 Fragments arranged by size: 3026 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5764 160 Size: 872 4732 1606 Fragments arranged by size: 4732 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7210 AvaI C'yCGr_G Cuts at: 1870 1943 2062 2068 2225 2311 2604 3856 4535 Size: 73 119 6 157 86 293 1252 679 Cuts at: 4535 1870 Size: 4545 Fragments arranged by size: 4545 1252 679 293 157 119 86 73 6 BamHI G'GATC_C Cuts at: 3145 4540 4907 3145 Size: 1395 367 5448 Fragments arranged by size: 5448 1395 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2168 2339 3457 3632 3833 Size: 325 217 627 381 171 1118 175 201 Cuts at: 3833 4211 4531 5986 618 Size: 378 320 1455 1842 Fragments arranged by size: 1842 1455 1118 627 381 378 325 320 217 201 175 171 BanII G_rGCy'C Cuts at: 1391 2069 2081 2238 2286 3061 3130 3289 3297 Size: 678 12 157 48 775 69 159 8 Cuts at: 3297 3314 3393 3697 3719 4440 4529 1391 Size: 17 79 304 22 721 89 4072 Fragments arranged by size: 4072 775 721 678 304 159 157 89 79 69 48 22 17 12 8 BbeI G_GCGC'C Cuts at: 2172 2172 Size: 7210 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3511 3520 928 Size: 971 1612 9 4618 Fragments arranged by size: 4618 1612 971 9 BbvCI CC'TCA_GC Cuts at: 2137 3765 3838 4049 2137 Size: 1628 73 211 5298 Fragments arranged by size: 5298 1628 211 73 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2025 2059 4435 4469 5401 5435 1903 Size: 34 88 34 2376 34 932 34 3678 Fragments arranged by size: 3678 2376 932 88 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5102 6312 5102 Size: 1210 6000 BclI T'GATC_A Cuts at: 1548 1548 Size: 7210 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2018 2114 Size: 107 122 71 631 605 337 9 96 Cuts at: 2114 3056 5821 136 Size: 942 2765 1525 Fragments arranged by size: 2765 1525 942 631 605 337 122 107 96 71 9 BglII A'GATC_T Cuts at: 7205 7205 Size: 7210 BlpI GC'TnA_GC Cuts at: 4627 4627 Size: 7210 Bme1580I G_kGCm'C Cuts at: 1790 2069 2286 3130 3297 3637 3719 4240 5271 Size: 279 217 844 167 340 82 521 1031 Cuts at: 5271 6427 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 1031 844 521 340 279 217 167 82 BmgBI CAC'GTC Cuts at: 1318 4348 1318 Size: 3030 4180 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2598 4941 5899 Size: 416 53 83 399 24 1181 2343 958 Cuts at: 5899 442 Size: 1753 Fragments arranged by size: 2343 1753 1181 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7210 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3853 3853 Size: 7210 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2201 2210 3008 3083 3833 4229 5870 1239 Size: 962 9 798 75 750 396 1641 2579 Fragments arranged by size: 2579 1641 962 798 750 396 75 9 Bpu10I CC'TnA_GC Cuts at: 2137 2278 3299 3421 3765 3805 3838 4049 2137 Size: 141 1021 122 344 40 33 211 5298 Fragments arranged by size: 5298 1021 344 211 141 122 40 33 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3116 3364 5335 6200 6498 3116 Size: 248 1971 865 298 3828 Fragments arranged by size: 3828 1971 865 298 248 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2522 3716 5873 882 Size: 617 1023 1194 2157 2219 Fragments arranged by size: 2219 2157 1194 1023 617 BsaAI yAC'GTr Cuts at: 493 1204 5001 493 Size: 711 3797 2702 Fragments arranged by size: 3797 2702 711 BsaBI GATnn'nnATC Cuts at: 2897 7204 2897 Size: 4307 2903 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2147 2169 2439 5017 Size: 53 83 186 936 614 22 270 2578 Cuts at: 5017 5399 6034 275 Size: 382 635 1451 Fragments arranged by size: 2578 1451 936 635 614 382 270 186 83 53 22 BsaWI w'CCGG_w Cuts at: 5643 6041 6315 6462 6873 5643 Size: 398 274 147 411 5980 Fragments arranged by size: 5980 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2507 2537 4241 4271 1178 Size: 30 165 30 1104 30 1704 30 4117 Fragments arranged by size: 4117 1704 1104 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3683 3686 4004 4049 1186 Size: 2497 3 318 45 4347 Fragments arranged by size: 4347 2497 318 45 3 BseYI C'CCAG_C Cuts at: 2485 6413 6909 2485 Size: 3928 496 2786 Fragments arranged by size: 3928 2786 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2227 2446 3104 3249 3359 3405 4083 Size: 573 491 219 658 145 110 46 678 Cuts at: 4083 4370 1163 Size: 287 4003 Fragments arranged by size: 4003 678 658 573 491 287 219 145 110 46 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2049 2118 4660 5421 6449 1540 Size: 275 186 48 69 2542 761 1028 2301 Fragments arranged by size: 2542 2301 1028 761 275 186 69 48 BsiHKAI G_wGCw'C Cuts at: 2196 2513 2825 3036 4020 5271 5356 6427 2196 Size: 317 312 211 984 1251 85 1071 2979 Fragments arranged by size: 2979 1251 1071 984 317 312 211 85 BsmI GAATG_Cn' Cuts at: 4721 4814 4721 Size: 93 7117 BsmBI CGTCTCn'nnnn_ Cuts at: 3668 4376 3668 Size: 708 6502 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2069 2081 2196 2238 2286 2513 2825 Size: 399 279 12 115 42 48 227 312 Cuts at: 2825 3036 3061 3130 3289 3297 3314 3393 3637 Size: 211 25 69 159 8 17 79 244 Cuts at: 3637 3697 3719 4020 4240 4440 4529 5271 5356 Size: 60 22 301 220 200 89 742 85 Cuts at: 5356 6427 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 312 301 279 244 227 220 211 200 159 115 89 85 79 69 60 48 42 25 22 17 12 8 BspEI T'CCGG_A Cuts at: 6873 6873 Size: 7210 BspHI T'CATG_A Cuts at: 2910 5099 6829 2910 Size: 2189 1730 3291 Fragments arranged by size: 3291 2189 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2696 3969 4565 844 Size: 1852 1273 596 3489 Fragments arranged by size: 3489 1852 1273 596 BsrBI CCG'CTC Cuts at: 1394 1823 2992 3420 3441 5097 1394 Size: 429 1169 428 21 1656 3507 Fragments arranged by size: 3507 1656 1169 429 428 21 BsrDI GCAATG_nn' Cuts at: 65 1248 2810 5705 5879 65 Size: 1183 1562 2895 174 1396 Fragments arranged by size: 2895 1562 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3596 3964 5854 6041 1700 Size: 110 1786 368 1890 187 2869 Fragments arranged by size: 2869 1890 1786 368 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7210 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7210 BssSI C'ACGA_G Cuts at: 3462 5270 6282 3462 Size: 1808 1012 4390 Fragments arranged by size: 4390 1808 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3040 794 Size: 2246 4964 BstBI TT'CG_AA Cuts at: 6014 6014 Size: 7210 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7210 BstXI CCAn_nnnn'nTGG Cuts at: 4208 4208 Size: 7210 BstYI r'GATC_y Cuts at: 1972 3015 3145 4219 4540 4907 5292 5309 6761 Size: 1043 130 1074 321 367 385 17 1452 Cuts at: 6761 6847 6859 7205 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1074 1043 385 367 346 321 130 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3828 3828 Size: 7210 BtgI C'CryG_G Cuts at: 513 1065 2004 2016 2142 2157 2286 2343 3150 Size: 552 939 12 126 15 129 57 807 Cuts at: 3150 3270 3330 513 Size: 120 60 4393 Fragments arranged by size: 4393 939 807 552 129 126 120 60 57 15 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6471 Fragments arranged by size: 6471 683 56 BtsI GCAGTG_nn' Cuts at: 2194 2754 4726 5520 5540 2194 Size: 560 1972 794 20 3864 Fragments arranged by size: 3864 1972 794 560 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7210 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7175 DraI TTT'AAA Cuts at: 4514 4514 Size: 7210 DraIII CAC_nnn'GTG Cuts at: 3791 3791 Size: 7210 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6217 809 Size: 507 4901 1802 Fragments arranged by size: 4901 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2046 2115 2895 Size: 54 1425 50 275 18 216 69 780 Cuts at: 2895 3598 3654 4083 4657 5546 7175 8 Size: 703 56 429 574 889 1629 43 Fragments arranged by size: 1629 1425 889 780 703 574 429 275 216 69 56 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2046 2115 4657 1537 Size: 275 234 69 2542 4090 Fragments arranged by size: 4090 2542 275 234 69 EarI CTCTTCn'nnn_ Cuts at: 1353 2813 5140 1353 Size: 1460 2327 3423 Fragments arranged by size: 3423 2327 1460 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2837 5797 6171 6317 387 Size: 1064 138 1248 2960 374 146 1280 Fragments arranged by size: 2960 1280 1248 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2201 2210 3008 3083 3833 4049 4229 Size: 393 569 9 798 75 750 216 180 Cuts at: 4229 5273 5870 6657 6925 1239 Size: 1044 597 787 268 1524 Fragments arranged by size: 1524 1044 798 787 750 597 569 393 268 216 180 75 9 EcoNI CCTnn'n_nnAGG Cuts at: 4031 4031 Size: 7210 EcoO109I rG'GnC_Cy Cuts at: 2282 3127 3184 3260 3293 3294 3715 3716 3755 Size: 845 57 76 33 1 421 1 39 Cuts at: 3755 3770 2282 Size: 15 5722 Fragments arranged by size: 5722 845 421 76 57 39 33 15 1 1 EcoRI G'AATT_C Cuts at: 4519 4897 4519 Size: 378 6832 FspI TGC'GCA Cuts at: 3452 5716 3452 Size: 2264 4946 HaeII r_GCGC'y Cuts at: 1678 2101 2172 2180 2218 2791 2986 3582 4375 Size: 423 71 8 38 573 195 596 793 Cuts at: 4375 6357 1678 Size: 1982 2531 Fragments arranged by size: 2531 1982 793 596 573 423 195 71 38 8 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3130 3162 3362 3394 4241 Size: 32 135 32 1558 32 200 32 847 Cuts at: 4241 4273 4337 4369 5851 5883 5925 5957 1373 Size: 32 64 32 1482 32 42 32 2626 Fragments arranged by size: 2626 1558 1482 847 200 135 64 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4329 4554 4800 669 Size: 988 281 2391 225 246 3079 Fragments arranged by size: 3079 2391 988 281 246 225 HindIII A'AGCT_T Cuts at: 748 748 Size: 7210 HpaI GTT'AAC Cuts at: 4329 4800 4329 Size: 471 6739 KasI G'GCGC_C Cuts at: 2168 2168 Size: 7210 KpnI G_GTAC'C Cuts at: 3837 4535 3837 Size: 698 6512 MfeI C'AATT_G Cuts at: 4809 4809 Size: 7210 MluI A'CGCG_T Cuts at: 7086 7086 Size: 7210 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6324 6508 1305 Size: 5019 184 2007 Fragments arranged by size: 5019 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2897 3656 4085 10 Size: 54 1425 343 1065 759 429 3135 Fragments arranged by size: 3135 1425 1065 759 429 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2345 2643 3335 4076 5168 5527 5686 Size: 648 1179 298 692 741 1092 359 159 Cuts at: 5686 518 Size: 2042 Fragments arranged by size: 2042 1179 1092 741 692 648 359 298 159 MspA1I CmG'CkG Cuts at: 1606 2018 2039 2084 2144 2159 2288 2551 3118 Size: 412 21 45 60 15 129 263 567 Cuts at: 3118 3500 3587 4108 4171 4626 5303 6451 6696 Size: 382 87 521 63 455 677 1148 245 Cuts at: 6696 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 567 521 455 412 382 263 245 129 87 63 60 45 21 15 NaeI GCC'GGC Cuts at: 3966 3966 Size: 7210 NarI GG'CG_CC Cuts at: 2169 2169 Size: 7210 NcoI C'CATG_G Cuts at: 513 1065 2004 3270 3330 513 Size: 552 939 1266 60 4393 Fragments arranged by size: 4393 1266 939 552 60 NdeI CA'TA_TG Cuts at: 387 387 Size: 7210 NgoMIV G'CCGG_C Cuts at: 3964 3964 Size: 7210 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7210 NotI GC'GGCC_GC Cuts at: 4657 4657 Size: 7210 NspI r_CATG'y Cuts at: 1132 1229 2807 4309 4568 6113 1132 Size: 97 1578 1502 259 1545 2229 Fragments arranged by size: 2229 1578 1545 1502 259 97 PasI CC'CwG_GG Cuts at: 1348 3290 1348 Size: 1942 5268 PciI A'CATG_T Cuts at: 6109 6109 Size: 7210 PflMI CCAn_nnn'nTGG Cuts at: 1108 3331 3851 4297 1108 Size: 2223 520 446 4021 Fragments arranged by size: 4021 2223 520 446 PfoI T'CCnGG_A Cuts at: 4245 4245 Size: 7210 PmeI GTTT'AAAC Cuts at: 4514 4514 Size: 7210 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5279 5311 6792 6824 5279 Size: 32 1481 32 5665 Fragments arranged by size: 5665 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3184 3755 3770 3184 Size: 571 15 6624 Fragments arranged by size: 6624 571 15 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7210 PsiI TTA'TAA Cuts at: 4780 4780 Size: 7210 PspOMI G'GGCC_C Cuts at: 2065 2282 3126 3293 3715 2065 Size: 217 844 167 422 5560 Fragments arranged by size: 5560 844 422 217 167 PstI C_TGCA'G Cuts at: 830 2907 3592 3622 3975 4371 4562 830 Size: 2077 685 30 353 396 191 3478 Fragments arranged by size: 3478 2077 685 396 353 191 30 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7210 PvuII CAG'CTG Cuts at: 3118 3500 4171 3118 Size: 382 671 6157 Fragments arranged by size: 6157 671 382 SacII CC_GC'GG Cuts at: 2019 2145 2160 2289 2019 Size: 126 15 129 6940 Fragments arranged by size: 6940 129 126 15 SalI G'TCGA_C Cuts at: 1936 4552 1936 Size: 2616 4594 SanDI GG'GwC_CC Cuts at: 3770 3770 Size: 7210 SapI GCTCTTCn'nnn_ Cuts at: 2813 2813 Size: 7210 SbfI CC_TGCA'GG Cuts at: 3622 3975 4562 3622 Size: 353 587 6270 Fragments arranged by size: 6270 587 353 ScaI AGT'ACT Cuts at: 5458 7170 5458 Size: 1712 5498 SfcI C'TryA_G Cuts at: 826 1045 2266 2903 3588 3618 3971 4267 4367 Size: 219 1221 637 685 30 353 296 100 Cuts at: 4367 4558 5693 6374 6565 826 Size: 191 1135 681 191 1471 Fragments arranged by size: 1471 1221 1135 685 681 637 353 296 219 191 191 100 30 SfoI GGC'GCC Cuts at: 2170 2170 Size: 7210 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7210 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7210 SmaI CCC'GGG Cuts at: 1872 2064 2070 3858 4537 1872 Size: 192 6 1788 679 4545 Fragments arranged by size: 4545 1788 679 192 6 SmlI C'TyrA_G Cuts at: 820 1017 1943 3131 3379 5314 6215 6477 820 Size: 197 926 1188 248 1935 901 262 1553 Fragments arranged by size: 1935 1553 1188 926 901 262 248 197 SnaBI TAC'GTA Cuts at: 493 5001 493 Size: 4508 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7210 SphI G_CATG'C Cuts at: 1132 4568 1132 Size: 3436 3774 SrfI GCCC'GGGC Cuts at: 2064 2070 2064 Size: 6 7204 SspI AAT'ATT Cuts at: 5 52 5134 5 Size: 47 5082 2081 Fragments arranged by size: 5082 2081 47 StuI AGG'CCT Cuts at: 1517 3215 4400 1517 Size: 1698 1185 4327 Fragments arranged by size: 4327 1698 1185 StyI C'CwwG_G Cuts at: 513 1065 2004 2565 2791 2826 3270 3330 4649 Size: 552 939 561 226 35 444 60 1319 Cuts at: 4649 513 Size: 3074 Fragments arranged by size: 3074 1319 939 561 552 444 226 60 35 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3283 5237 5254 5407 5592 6037 325 Size: 1255 1703 1954 17 153 185 445 1498 Fragments arranged by size: 1954 1703 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2559 4123 5456 Size: 274 80 33 51 1443 582 1564 1333 Cuts at: 5456 7168 96 Size: 1712 138 Fragments arranged by size: 1712 1564 1443 1333 582 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2507 2539 3190 3222 7170 7202 2507 Size: 32 651 32 3948 32 2515 Fragments arranged by size: 3948 2515 651 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7210 XbaI T'CTAG_A Cuts at: 4546 4546 Size: 7210 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2389 2500 3808 3990 4192 4484 2389 Size: 111 1308 182 202 292 5115 Fragments arranged by size: 5115 1308 292 202 182 111 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7210 XmaI C'CCGG_G Cuts at: 1870 2062 2068 3856 4535 1870 Size: 192 6 1788 679 4545 Fragments arranged by size: 4545 1788 679 192 6 XmnI GAAnn'nnTTC Cuts at: 2402 5339 2402 Size: 2937 4273 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5018 276 Size: 53 83 186 936 3484 2468 Fragments arranged by size: 3484 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SexAI SfiI SwaI