Restriction map for pFN21AB7016
 (Linear) (Six-base) MAPSORT of: KIBB7016.seq  Check: 8725  from: 1  to: 2505



KIBB7016.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:28  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     691    1964    2505 
   Size:        691    1273     541

  Fragments arranged by size: 

                1273     691     541
 
Acc65I G'GTAC_C

Cuts at:      0    1828    2505 
   Size:       1828     677
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2044    2505 
   Size:       2044     461
 
AfeI AGC'GCT

Cuts at:      0      94    2368    2505 
   Size:         94    2274     137

  Fragments arranged by size: 

                2274     137      94
 
AhdI GACnn_n'nnGTC

Cuts at:      0    2346    2505 
   Size:       2346     159
 
AleI CACnn'nnGTG

Cuts at:      0     340    2071    2505 
   Size:        340    1731     434

  Fragments arranged by size: 

                1731     434     340
 
AlwNI CAG_nnn'CTG

Cuts at:      0     494    1258    1606    2478    2505 
   Size:        494     764     348     872      27

  Fragments arranged by size: 

                 872     764     494     348      27
 
ApaI G_GGCC'C

Cuts at:      0      64     281    1125    1292    1714    2505 
   Size:         64     217     844     167     422     791

  Fragments arranged by size: 

                 844     791     422     217     167      64
 
AvaI C'yCGr_G

Cuts at:      0      57      63     220     306     599    1851    2505 
   Size:         57       6     157      86     293    1252     654

  Fragments arranged by size: 

                1252     654     293     157      86      57       6
 
BamHI G'GATC_C

Cuts at:      0    1140    2505 
   Size:       1140    1365
 
BanI G'GyrC_C

Cuts at:      0     163     334    1452    1627    1828    2206    2505 
   Size:        163     171    1118     175     201     378     299

  Fragments arranged by size: 

                1118     378     299     201     175     171     163
 
BanII G_rGCy'C

Cuts at:      0      64      76     233     281    1056    1125    1284    1292 
   Size:         64      12     157      48     775      69     159       8

Cuts at:   1292    1309    1388    1692    1714    2435    2505 
   Size:         17      79     304      22     721      70

  Fragments arranged by size: 

                 775     721     304     159     157      79      70      69
                  64      48      22      17      12       8
 
BbeI G_GCGC'C

Cuts at:      0     167    2505 
   Size:        167    2338
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1506    1515    2505 
   Size:       1506       9     990

  Fragments arranged by size: 

                1506     990       9
 
BbvCI CC'TCA_GC

Cuts at:      0     132    1760    1833    2044    2505 
   Size:        132    1628      73     211     461

  Fragments arranged by size: 

                1628     461     211     132      73
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      20      54    2430    2464    2505 
   Size:         20      34    2376      34      41

  Fragments arranged by size: 

                2376      41      34      34      20
 
BglI GCCn_nnn'nGGC

Cuts at:      0      13     109    1051    2505 
   Size:         13      96     942    1454

  Fragments arranged by size: 

                1454     942      96      13
 
Bme1580I G_kGCm'C

Cuts at:      0      64     281    1125    1292    1632    1714    2235    2505 
   Size:         64     217     844     167     340      82     521     270

  Fragments arranged by size: 

                 844     521     340     270     217     167      82      64
            
 
BmgBI CAC'GTC

Cuts at:      0    2343    2505 
   Size:       2343     162
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     593    2505 
   Size:        593    1912
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1848    2505 
   Size:       1848     657
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     196     205    1003    1078    1828    2224    2505 
   Size:        196       9     798      75     750     396     281

  Fragments arranged by size: 

                 798     750     396     281     196      75       9
 
Bpu10I CC'TnA_GC

Cuts at:      0     132     273    1294    1416    1760    1800    1833    2044 
   Size:        132     141    1021     122     344      40      33     211

Cuts at:   2044    2505 
   Size:        461

  Fragments arranged by size: 

                1021     461     344     211     141     132     122      40
                  33
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1111    1359    2505 
   Size:       1111     248    1146

  Fragments arranged by size: 

                1146    1111     248
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     517    1711    2505 
   Size:        517    1194     794

  Fragments arranged by size: 

                1194     794     517
 
BsaBI GATnn'nnATC

Cuts at:      0     892    2505 
   Size:        892    1613
 
BsaHI Gr'CG_yC

Cuts at:      0     142     164     434    2505 
   Size:        142      22     270    2071

  Fragments arranged by size: 

                2071     270     142      22
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     502     532    2236    2266    2505 
   Size:        502      30    1704      30     239

  Fragments arranged by size: 

                1704     502     239      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1678    1681    1999    2044    2505 
   Size:       1678       3     318      45     461

  Fragments arranged by size: 

                1678     461     318      45       3
 
BseYI C'CCAG_C

Cuts at:      0     480    2505 
   Size:        480    2025
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     222     441    1099    1244    1354    1400    2078    2365 
   Size:        222     219     658     145     110      46     678     287

Cuts at:   2365    2505 
   Size:        140

  Fragments arranged by size: 

                 678     658     287     222     219     145     140     110
                  46
 
BsiEI CG_ry'CG

Cuts at:      0      44     113    2505 
   Size:         44      69    2392

  Fragments arranged by size: 

                2392      69      44
 
BsiHKAI G_wGCw'C

Cuts at:      0     191     508     820    1031    2015    2505 
   Size:        191     317     312     211     984     490

  Fragments arranged by size: 

                 984     490     317     312     211     191
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1663    2371    2505 
   Size:       1663     708     134

  Fragments arranged by size: 

                1663     708     134
 
Bsp1286I G_dGCh'C

Cuts at:      0      64      76     191     233     281     508     820    1031 
   Size:         64      12     115      42      48     227     312     211

Cuts at:   1031    1056    1125    1284    1292    1309    1388    1632    1692 
   Size:         25      69     159       8      17      79     244      60

Cuts at:   1692    1714    2015    2235    2435    2505 
   Size:         22     301     220     200      70

  Fragments arranged by size: 

                 312     301     244     227     220     211     200     159
                 115      79      70      69      64      60      48      42
                  25      22      17      12       8
 
BspHI T'CATG_A

Cuts at:      0     905    2505 
   Size:        905    1600
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     691    1964    2505 
   Size:        691    1273     541

  Fragments arranged by size: 

                1273     691     541
 
BsrBI CCG'CTC

Cuts at:      0     987    1415    1436    2505 
   Size:        987     428      21    1069

  Fragments arranged by size: 

                1069     987     428      21
 
BsrDI GCAATG_nn'

Cuts at:      0     805    2505 
   Size:        805    1700
 
BsrFI r'CCGG_y

Cuts at:      0    1591    1959    2505 
   Size:       1591     368     546

  Fragments arranged by size: 

                1591     546     368
 
BssSI C'ACGA_G

Cuts at:      0    1457    2505 
   Size:       1457    1048
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1035    2505 
   Size:       1035    1470
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2203    2505 
   Size:       2203     302
 
BstYI r'GATC_y

Cuts at:      0    1010    1140    2214    2505 
   Size:       1010     130    1074     291

  Fragments arranged by size: 

                1074    1010     291     130
 
Bsu36I CC'TnA_GG

Cuts at:      0    1823    2505 
   Size:       1823     682
 
BtgI C'CryG_G

Cuts at:      0      11     137     152     281     338    1145    1265    1325 
   Size:         11     126      15     129      57     807     120      60

Cuts at:   1325    2505 
   Size:       1180

  Fragments arranged by size: 

                1180     807     129     126     120      60      57      15
                  11
 
BtsI GCAGTG_nn'

Cuts at:      0     189     749    2505 
   Size:        189     560    1756

  Fragments arranged by size: 

                1756     560     189
 
DraIII CAC_nnn'GTG

Cuts at:      0    1786    2505 
   Size:       1786     719
 
EaeI y'GGCC_r

Cuts at:      0      41     110     890    1593    1649    2078    2505 
   Size:         41      69     780     703      56     429     427

  Fragments arranged by size: 

                 780     703     429     427      69      56      41
 
EagI C'GGCC_G

Cuts at:      0      41     110    2505 
   Size:         41      69    2395

  Fragments arranged by size: 

                2395      69      41
 
EarI CTCTTCn'nnn_

Cuts at:      0     808    2505 
   Size:        808    1697
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     832    2505 
   Size:        832    1673
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     196     205    1003    1078    1828    2044    2224    2505 
   Size:        196       9     798      75     750     216     180     281

  Fragments arranged by size: 

                 798     750     281     216     196     180      75       9
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2026    2505 
   Size:       2026     479
 
EcoO109I rG'GnC_Cy

Cuts at:      0     277    1122    1179    1255    1288    1289    1710    1711 
   Size:        277     845      57      76      33       1     421       1

Cuts at:   1711    1750    1765    2505 
   Size:         39      15     740

  Fragments arranged by size: 

                 845     740     421     277      76      57      39      33
                  15       1       1
 
FspI TGC'GCA

Cuts at:      0    1447    2505 
   Size:       1447    1058
 
HaeII r_GCGC'y

Cuts at:      0      96     167     175     213     786     981    1577    2370 
   Size:         96      71       8      38     573     195     596     793

Cuts at:   2370    2505 
   Size:        135

  Fragments arranged by size: 

                 793     596     573     195     135      96      71      38
                   8
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1125    1157    1357    1389    2236    2268    2332    2364 
   Size:       1125      32     200      32     847      32      64      32

Cuts at:   2364    2505 
   Size:        141

  Fragments arranged by size: 

                1125     847     200     141      64      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0    2324    2505 
   Size:       2324     181
 
HpaI GTT'AAC

Cuts at:      0    2324    2505 
   Size:       2324     181
 
KasI G'GCGC_C

Cuts at:      0     163    2505 
   Size:        163    2342
 
KpnI G_GTAC'C

Cuts at:      0    1832    2505 
   Size:       1832     673
 
MscI TGG'CCA

Cuts at:      0     892    1651    2080    2505 
   Size:        892     759     429     425

  Fragments arranged by size: 

                 892     759     429     425
 
MslI CAynn'nnrTG

Cuts at:      0     340     638    1330    2071    2505 
   Size:        340     298     692     741     434

  Fragments arranged by size: 

                 741     692     434     340     298
 
MspA1I CmG'CkG

Cuts at:      0      13      34      79     139     154     283     546    1113 
   Size:         13      21      45      60      15     129     263     567

Cuts at:   1113    1495    1582    2103    2166    2505 
   Size:        382      87     521      63     339

  Fragments arranged by size: 

                 567     521     382     339     263     129      87      63
                  60      45      21      15      13
 
NaeI GCC'GGC

Cuts at:      0    1961    2505 
   Size:       1961     544
 
NarI GG'CG_CC

Cuts at:      0     164    2505 
   Size:        164    2341
 
NcoI C'CATG_G

Cuts at:      0    1265    1325    2505 
   Size:       1265      60    1180

  Fragments arranged by size: 

                1265    1180      60
 
NgoMIV G'CCGG_C

Cuts at:      0    1959    2505 
   Size:       1959     546
 
NspI r_CATG'y

Cuts at:      0     802    2304    2505 
   Size:        802    1502     201

  Fragments arranged by size: 

                1502     802     201
 
PasI CC'CwG_GG

Cuts at:      0    1285    2505 
   Size:       1285    1220
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1326    1846    2292    2505 
   Size:       1326     520     446     213

  Fragments arranged by size: 

                1326     520     446     213
 
PfoI T'CCnGG_A

Cuts at:      0    2240    2505 
   Size:       2240     265
 
PpuMI rG'GwC_Cy

Cuts at:      0    1179    1750    1765    2505 
   Size:       1179     571      15     740

  Fragments arranged by size: 

                1179     740     571      15
 
PspOMI G'GGCC_C

Cuts at:      0      60     277    1121    1288    1710    2505 
   Size:         60     217     844     167     422     795

  Fragments arranged by size: 

                 844     795     422     217     167      60
 
PstI C_TGCA'G

Cuts at:      0     902    1587    1617    1970    2366    2505 
   Size:        902     685      30     353     396     139

  Fragments arranged by size: 

                 902     685     396     353     139      30
 
PvuII CAG'CTG

Cuts at:      0    1113    1495    2166    2505 
   Size:       1113     382     671     339

  Fragments arranged by size: 

                1113     671     382     339
 
SacII CC_GC'GG

Cuts at:      0      14     140     155     284    2505 
   Size:         14     126      15     129    2221

  Fragments arranged by size: 

                2221     129     126      15      14
 
SanDI GG'GwC_CC

Cuts at:      0    1765    2505 
   Size:       1765     740
 
SapI GCTCTTCn'nnn_

Cuts at:      0     808    2505 
   Size:        808    1697
 
SbfI CC_TGCA'GG

Cuts at:      0    1617    1970    2505 
   Size:       1617     353     535

  Fragments arranged by size: 

                1617     535     353
 
SfcI C'TryA_G

Cuts at:      0     261     898    1583    1613    1966    2262    2362    2505 
   Size:        261     637     685      30     353     296     100     143

  Fragments arranged by size: 

                 685     637     353     296     261     143     100      30
            
 
SfoI GGC'GCC

Cuts at:      0     165    2505 
   Size:        165    2340
 
SmaI CCC'GGG

Cuts at:      0      59      65    1853    2505 
   Size:         59       6    1788     652

  Fragments arranged by size: 

                1788     652      59       6
 
SmlI C'TyrA_G

Cuts at:      0    1126    1374    2505 
   Size:       1126     248    1131

  Fragments arranged by size: 

                1131    1126     248
 
SrfI GCCC'GGGC

Cuts at:      0      59      65    2505 
   Size:         59       6    2440

  Fragments arranged by size: 

                2440      59       6
 
StuI AGG'CCT

Cuts at:      0    1210    2395    2505 
   Size:       1210    1185     110

  Fragments arranged by size: 

                1210    1185     110
 
StyI C'CwwG_G

Cuts at:      0     560     786     821    1265    1325    2505 
   Size:        560     226      35     444      60    1180

  Fragments arranged by size: 

                1180     560     444     226      60      35
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1278    2505 
   Size:       1278    1227
 
TatI w'GTAC_w

Cuts at:      0     554    2118    2505 
   Size:        554    1564     387

  Fragments arranged by size: 

                1564     554     387
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     502     534    1185    1217    2505 
   Size:        502      32     651      32    1288

  Fragments arranged by size: 

                1288     651     502      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     384     495    1803    1985    2187    2479    2505 
   Size:        384     111    1308     182     202     292      26

  Fragments arranged by size: 

                1308     384     292     202     182     111      26
 
XmaI C'CCGG_G

Cuts at:      0      57      63    1851    2505 
   Size:         57       6    1788     654

  Fragments arranged by size: 

                1788     654      57       6
 
XmnI GAAnn'nnTTC

Cuts at:      0     397    2505 
   Size:        397    2108
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI     AfeI     AhdI     AleI    AlwNI     ApaI
     AvaI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI
     BglI Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaBI    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI    BsmBI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BssSI   BstAPI    BstXI    BstYI   Bsu36I     BtgI     BtsI   DraIII
     EaeI     EagI     EarI     EciI  Eco57MI    EcoNI EcoO109I     FspI
    HaeII    Hin4I   HincII     HpaI     KasI     KpnI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PasI    PflMI
     PfoI    PpuMI   PspOMI     PstI    PvuII    SacII    SanDI     SapI
     SbfI     SfcI     SfoI     SmaI     SmlI     SrfI     StuI     StyI
    TaqII     TatI     TstI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI     AclI    AflII   AflIII     AgeI     AlfI     AloI
    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BciVI
     BclI    BfrBI    BglII     BlpI     BmtI    BsaAI    BsaWI    BsiWI
     BsmI    BspEI    BsrGI   BssHII    BstBI   BstEII  BstZ17I    BtgZI
     ClaI    CspCI     DraI     DrdI  EcoICRI    EcoRI    EcoRV     FalI
     FseI    FspAI  HindIII     MfeI     MluI     MmeI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PciI     PmeI     PmlI     PpiI
    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII     SacI     SalI
     ScaI    SexAI     SfiI     SgfI    SgrAI    SnaBI     SpeI     SphI
     SspI     SwaI  Tth111I     XbaI     XhoI     ZraI
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