Restriction map for
pFN21AB7005
(Linear) (Six-base) MAPSORT of: KIBB7005.seq Check: 3092 from: 1 to: 3777
KIBB7005.orf
With 182 enzymes: SgfI *
February 28, 2007 11:28 ..
AatII G_ACGT'C
Cuts at: 0 1951 3485 3777
Size: 1951 1534 292
Fragments arranged by size:
1951 1534 292
AccI GT'mk_AC
Cuts at: 0 1405 2257 2522 3136 3350 3572 3777
Size: 1405 852 265 614 214 222 205
Fragments arranged by size:
1405 852 614 265 222 214 205
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 364 1045 1676 1875 2642 2805 2817 3777
Size: 364 681 631 199 767 163 12 960
Fragments arranged by size:
960 767 681 631 364 199 163 12
AflIII A'CryG_T
Cuts at: 0 2336 3777
Size: 2336 1441
AgeI A'CCGG_T
Cuts at: 0 2944 3777
Size: 2944 833
AhdI GACnn_n'nnGTC
Cuts at: 0 3564 3777
Size: 3564 213
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 967 1769 2614 3777
Size: 967 802 845 1163
Fragments arranged by size:
1163 967 845 802
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2971 3003 3777
Size: 2971 32 774
Fragments arranged by size:
2971 774 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1023 3103 3412 3684 3777
Size: 1023 2080 309 272 93
Fragments arranged by size:
2080 1023 309 272 93
ApaLI G'TGCA_C
Cuts at: 0 942 3777
Size: 942 2835
ApoI r'AATT_y
Cuts at: 0 81 2629 2895 3145 3159 3777
Size: 81 2548 266 250 14 618
Fragments arranged by size:
2548 618 266 250 81 14
AvaI C'yCGr_G
Cuts at: 0 1975 3416 3714 3777
Size: 1975 1441 298 63
Fragments arranged by size:
1975 1441 298 63
AvrII C'CTAG_G
Cuts at: 0 591 3777
Size: 591 3186
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 278 311 3777
Size: 278 33 3466
Fragments arranged by size:
3466 278 33
BanI G'GyrC_C
Cuts at: 0 793 2167 3777
Size: 793 1374 1610
Fragments arranged by size:
1610 1374 793
BanII G_rGCy'C
Cuts at: 0 165 2290 2354 2708 3777
Size: 165 2125 64 354 1069
Fragments arranged by size:
2125 1069 354 165 64
BbsI GAAGACnn'nnnn_
Cuts at: 0 1924 2537 3693 3777
Size: 1924 613 1156 84
Fragments arranged by size:
1924 1156 613 84
BbvCI CC'TCA_GC
Cuts at: 0 1532 2179 3777
Size: 1532 647 1598
Fragments arranged by size:
1598 1532 647
BciVI GTATCCnnnnn_n'
Cuts at: 0 2007 3777
Size: 2007 1770
BclI T'GATC_A
Cuts at: 0 3254 3777
Size: 3254 523
BfrBI ATG'CAT
Cuts at: 0 1746 3777
Size: 1746 2031
BglII A'GATC_T
Cuts at: 0 912 1242 1449 1767 3191 3777
Size: 912 330 207 318 1424 586
Fragments arranged by size:
1424 912 586 330 318 207
BlpI GC'TnA_GC
Cuts at: 0 1283 2467 3777
Size: 1283 1184 1310
Fragments arranged by size:
1310 1283 1184
Bme1580I G_kGCm'C
Cuts at: 0 946 1128 1545 2172 3721 3777
Size: 946 182 417 627 1549 56
Fragments arranged by size:
1549 946 627 417 182 56
BmgBI CAC'GTC
Cuts at: 0 2337 3756 3777
Size: 2337 1419 21
Fragments arranged by size:
2337 1419 21
BmrI ACTGGGnnnn_n'
Cuts at: 0 2084 3375 3777
Size: 2084 1291 402
Fragments arranged by size:
2084 1291 402
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2142 2160 3777
Size: 2142 18 1617
Fragments arranged by size:
2142 1617 18
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 92 669 1108 2041 2179 3265 3693 3777
Size: 92 577 439 933 138 1086 428 84
Fragments arranged by size:
1086 933 577 439 428 138 92 84
Bpu10I CC'TnA_GC
Cuts at: 0 1532 2179 3777
Size: 1532 647 1598
Fragments arranged by size:
1598 1532 647
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 642 3022 3595 3777
Size: 642 2380 573 182
Fragments arranged by size:
2380 642 573 182
BsaI GGTCTCn'nnnn_
Cuts at: 0 1972 3777
Size: 1972 1805
BsaAI yAC'GTr
Cuts at: 0 1803 1863 2175 2193 2364 2622 3777
Size: 1803 60 312 18 171 258 1155
Fragments arranged by size:
1803 1155 312 258 171 60 18
BsaBI GATnn'nnATC
Cuts at: 0 2930 3777
Size: 2930 847
BsaHI Gr'CG_yC
Cuts at: 0 5 722 749 1948 3482 3777
Size: 5 717 27 1199 1534 295
Fragments arranged by size:
1534 1199 717 295 27 5
BsaWI w'CCGG_w
Cuts at: 0 1913 2561 2944 3777
Size: 1913 648 383 833
Fragments arranged by size:
1913 833 648 383
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 642 672 2834 2864 3777
Size: 642 30 2162 30 913
Fragments arranged by size:
2162 913 642 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 28 223 694 1675 2118 2176 3513 3777
Size: 28 195 471 981 443 58 1337 264
Fragments arranged by size:
1337 981 471 443 264 195 58 28
BseYI C'CCAG_C
Cuts at: 0 983 1067 1538 2354 2664 2691 2990 3096
Size: 983 84 471 816 310 27 299 106
Cuts at: 3096 3739 3777
Size: 643 38
Fragments arranged by size:
983 816 643 471 310 299 106 84
38 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 751 988 1036 2205 2344 2347 3500 3777
Size: 751 237 48 1169 139 3 1153 277
Fragments arranged by size:
1169 1153 751 277 237 139 48 3
BsiEI CG_ry'CG
Cuts at: 0 3692 3777
Size: 3692 85
BsiHKAI G_wGCw'C
Cuts at: 0 494 946 1059 1515 3653 3777
Size: 494 452 113 456 2138 124
Fragments arranged by size:
2138 494 456 452 124 113
BsmI GAATG_Cn'
Cuts at: 0 543 1746 2396 3630 3777
Size: 543 1203 650 1234 147
Fragments arranged by size:
1234 1203 650 543 147
Bsp1286I G_dGCh'C
Cuts at: 0 165 494 946 1059 1128 1515 1545 2172
Size: 165 329 452 113 69 387 30 627
Cuts at: 2172 2290 2354 2708 3653 3721 3777
Size: 118 64 354 945 68 56
Fragments arranged by size:
945 627 452 387 354 329 165 118
113 69 68 64 56 30
BspEI T'CCGG_A
Cuts at: 0 2561 3777
Size: 2561 1216
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1261 1622 2969 3777
Size: 1261 361 1347 808
Fragments arranged by size:
1347 1261 808 361
BsrBI CCG'CTC
Cuts at: 0 3453 3777
Size: 3453 324
BsrDI GCAATG_nn'
Cuts at: 0 562 1142 2478 3069 3777
Size: 562 580 1336 591 708
Fragments arranged by size:
1336 708 591 580 562
BsrFI r'CCGG_y
Cuts at: 0 27 2428 2944 3074 3687 3691 3777
Size: 27 2401 516 130 613 4 86
Fragments arranged by size:
2401 613 516 130 86 27 4
BsrGI T'GTAC_A
Cuts at: 0 1835 3777
Size: 1835 1942
BssHII G'CGCG_C
Cuts at: 0 987 3777
Size: 987 2790
BssSI C'ACGA_G
Cuts at: 0 2532 2586 3258 3777
Size: 2532 54 672 519
Fragments arranged by size:
2532 672 519 54
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3684 3777
Size: 3684 93
BstEII G'GTnAC_C
Cuts at: 0 2751 3777
Size: 2751 1026
BstXI CCAn_nnnn'nTGG
Cuts at: 0 140 867 1794 3408 3777
Size: 140 727 927 1614 369
Fragments arranged by size:
1614 927 727 369 140
BstYI r'GATC_y
Cuts at: 0 912 1242 1449 1767 3191 3777
Size: 912 330 207 318 1424 586
Fragments arranged by size:
1424 912 586 330 318 207
BstZ17I GTA'TAC
Cuts at: 0 1406 2258 3137 3777
Size: 1406 852 879 640
Fragments arranged by size:
1406 879 852 640
Bsu36I CC'TnA_GG
Cuts at: 0 2145 2859 3777
Size: 2145 714 918
Fragments arranged by size:
2145 918 714
BtgI C'CryG_G
Cuts at: 0 41 970 1793 2785 3777
Size: 41 929 823 992 992
Fragments arranged by size:
992 992 929 823 41
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2793 3777
Size: 2793 984
BtsI GCAGTG_nn'
Cuts at: 0 3377 3682 3777
Size: 3377 305 95
Fragments arranged by size:
3377 305 95
ClaI AT'CG_AT
Cuts at: 0 883 3777
Size: 883 2894
DraI TTT'AAA
Cuts at: 0 3150 3777
Size: 3150 627
DraIII CAC_nnn'GTG
Cuts at: 0 329 1054 1155 2769 3777
Size: 329 725 101 1614 1008
Fragments arranged by size:
1614 1008 725 329 101
EaeI y'GGCC_r
Cuts at: 0 156 1574 2430 2739 3689 3693 3777
Size: 156 1418 856 309 950 4 84
Fragments arranged by size:
1418 950 856 309 156 84 4
EagI C'GGCC_G
Cuts at: 0 3689 3777
Size: 3689 88
EarI CTCTTCn'nnn_
Cuts at: 0 519 625 760 823 961 1652 3044 3460
Size: 519 106 135 63 138 691 1392 416
Cuts at: 3460 3777
Size: 317
Fragments arranged by size:
1392 691 519 416 317 138 135 106
63
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2518 3777
Size: 2518 1259
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 92 364 669 1045 1108 1676 1875 2041
Size: 92 272 305 376 63 568 199 166
Cuts at: 2041 2179 2642 2805 2817 3265 3693 3777
Size: 138 463 163 12 448 428 84
Fragments arranged by size:
568 463 448 428 376 305 272 199
166 163 138 92 84 63 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1531 2497 3277 3777
Size: 1531 966 780 500
Fragments arranged by size:
1531 966 780 500
EcoO109I rG'GnC_Cy
Cuts at: 0 149 1228 3777
Size: 149 1079 2549
Fragments arranged by size:
2549 1079 149
FseI GG_CCGG'CC
Cuts at: 0 3695 3777
Size: 3695 82
FspI TGC'GCA
Cuts at: 0 919 3777
Size: 919 2858
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 833 865 2326 2358 2580 2612 3550 3582
Size: 833 32 1461 32 222 32 938 32
Cuts at: 3582 3777
Size: 195
Fragments arranged by size:
1461 938 833 222 195 32 32 32
32
HincII GTy'rAC
Cuts at: 0 774 1260 3351 3777
Size: 774 486 2091 426
Fragments arranged by size:
2091 774 486 426
HindIII A'AGCT_T
Cuts at: 0 1355 3331 3777
Size: 1355 1976 446
Fragments arranged by size:
1976 1355 446
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 521 1422 2552 2600 3066 3777
Size: 127 394 901 1130 48 466 711
Fragments arranged by size:
1130 901 711 466 394 127 48
MslI CAynn'nnrTG
Cuts at: 0 865 1399 3400 3406 3442 3777
Size: 865 534 2001 6 36 335
Fragments arranged by size:
2001 865 534 335 36 6
MspA1I CmG'CkG
Cuts at: 0 21 1572 2271 2358 2373 2744 2919 3114
Size: 21 1551 699 87 15 371 175 195
Cuts at: 3114 3545 3777
Size: 431 232
Fragments arranged by size:
1551 699 431 371 232 195 175 87
21 15
NaeI GCC'GGC
Cuts at: 0 3689 3693 3777
Size: 3689 4 84
Fragments arranged by size:
3689 84 4
NcoI C'CATG_G
Cuts at: 0 41 3777
Size: 41 3736
NgoMIV G'CCGG_C
Cuts at: 0 3687 3691 3777
Size: 3687 4 86
Fragments arranged by size:
3687 86 4
NsiI A_TGCA'T
Cuts at: 0 1748 3777
Size: 1748 2029
NspI r_CATG'y
Cuts at: 0 2103 2691 3777
Size: 2103 588 1086
Fragments arranged by size:
2103 1086 588
PflMI CCAn_nnn'nTGG
Cuts at: 0 1155 3759 3777
Size: 1155 2604 18
Fragments arranged by size:
2604 1155 18
PfoI T'CCnGG_A
Cuts at: 0 532 3758 3777
Size: 532 3226 19
Fragments arranged by size:
3226 532 19
PmlI CAC'GTG
Cuts at: 0 1803 1863 2175 2193 2364 2622 3777
Size: 1803 60 312 18 171 258 1155
Fragments arranged by size:
1803 1155 312 258 171 60 18
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1013 1045 3777
Size: 1013 32 2732
Fragments arranged by size:
2732 1013 32
PpuMI rG'GwC_Cy
Cuts at: 0 149 1228 3777
Size: 149 1079 2549
Fragments arranged by size:
2549 1079 149
PshAI GACnn'nnGTC
Cuts at: 0 1215 3777
Size: 1215 2562
PsiI TTA'TAA
Cuts at: 0 1322 3777
Size: 1322 2455
PstI C_TGCA'G
Cuts at: 0 710 965 1619 2089 2465 3643 3680 3777
Size: 710 255 654 470 376 1178 37 97
Fragments arranged by size:
1178 710 654 470 376 255 97 37
PvuII CAG'CTG
Cuts at: 0 1572 2271 2358 2919 3114 3545 3777
Size: 1572 699 87 561 195 431 232
Fragments arranged by size:
1572 699 561 431 232 195 87
SalI G'TCGA_C
Cuts at: 0 3349 3777
Size: 3349 428
SapI GCTCTTCn'nnn_
Cuts at: 0 823 961 3044 3460 3777
Size: 823 138 2083 416 317
Fragments arranged by size:
2083 823 416 317 138
SexAI A'CCwGG_T
Cuts at: 0 1050 3777
Size: 1050 2727
SfcI C'TryA_G
Cuts at: 0 305 706 961 1615 2085 2461 3003 3639
Size: 305 401 255 654 470 376 542 636
Cuts at: 3639 3676 3777
Size: 37 101
Fragments arranged by size:
654 636 542 470 401 376 305 255
101 37
SgrAI Cr'CCGG_yG
Cuts at: 0 27 3777
Size: 27 3750
SmlI C'TyrA_G
Cuts at: 0 621 3037 3610 3777
Size: 621 2416 573 167
Fragments arranged by size:
2416 621 573 167
SpeI A'CTAG_T
Cuts at: 0 2967 3777
Size: 2967 810
SspI AAT'ATT
Cuts at: 0 664 3777
Size: 664 3113
StuI AGG'CCT
Cuts at: 0 3659 3777
Size: 3659 118
StyI C'CwwG_G
Cuts at: 0 41 591 1193 1593 2240 3777
Size: 41 550 602 400 647 1537
Fragments arranged by size:
1537 647 602 550 400 41
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1059 3777
Size: 1059 2718
TatI w'GTAC_w
Cuts at: 0 1835 2339 3777
Size: 1835 504 1438
Fragments arranged by size:
1835 1438 504
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 642 674 1933 1965 2834 2866 3025 3057
Size: 642 32 1259 32 869 32 159 32
Cuts at: 3057 3777
Size: 720
Fragments arranged by size:
1259 869 720 642 159 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 2581 3754 3777
Size: 2581 1173 23
Fragments arranged by size:
2581 1173 23
XbaI T'CTAG_A
Cuts at: 0 2133 3777
Size: 2133 1644
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 830 3777
Size: 830 2947
XmnI GAAnn'nnTTC
Cuts at: 0 541 3777
Size: 541 3236
ZraI GAC'GTC
Cuts at: 0 1949 3483 3777
Size: 1949 1534 294
Fragments arranged by size:
1949 1534 294
Enzymes that do cut:
AatII AccI AcuI AflIII AgeI AhdI AlfI AloI
AlwNI ApaLI ApoI AvaI AvrII BaeI BanI BanII
BbsI BbvCI BciVI BclI BfrBI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI DraI DraIII EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I FseI FspI
Hin4I HincII HindIII MmeI MslI MspA1I NaeI NcoI
NgoMIV NsiI NspI PflMI PfoI PmlI PpiI PpuMI
PshAI PsiI PstI PvuII SalI SapI SexAI SfcI
SgrAI SmlI SpeI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AleI ApaI AscI
AseI AsiSI BamHI BbeI BcgI BglI BmtI BsiWI
BsmBI BspHI BstBI CspCI DrdI EcoICRI EcoRI EcoRV
FalI FspAI HaeII HpaI KasI KpnI MfeI MluI
MscI NarI NdeI NheI NotI NruI PacI PasI
PciI PmeI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SanDI SbfI ScaI SfiI SfoI SgfI SmaI
SnaBI SphI SrfI SwaI XhoI XmaI