(Circular) (Six-base) MAPSORT of: pFN21AB5702.seq Check: 5841 from: 1 to: 7423 pFN21AB5702 7423 bp With 182 enzymes: SgfI * February 27, 2009 14:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5233 278 Size: 53 83 186 936 3697 2468 Fragments arranged by size: 3697 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2445 3142 3705 4766 1937 Size: 508 697 563 1061 4594 Fragments arranged by size: 4594 1061 697 563 508 Acc65I G'GTAC_C Cuts at: 4744 4744 Size: 7423 AclI AA'CG_TT Cuts at: 1563 2655 5550 5923 1563 Size: 1092 2895 373 3063 Fragments arranged by size: 3063 2895 1092 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2571 2875 4125 5486 6870 7138 1632 Size: 939 304 1250 1361 1384 268 1917 Fragments arranged by size: 1917 1384 1361 1250 939 304 268 AfeI AGC'GCT Cuts at: 2903 2903 Size: 7423 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7226 AflIII A'CryG_T Cuts at: 1203 2978 6322 7299 1203 Size: 1775 3344 977 1327 Fragments arranged by size: 3344 1775 1327 977 AgeI A'CCGG_T Cuts at: 6254 6254 Size: 7423 AhdI GACnn_n'nnGTC Cuts at: 6152 6152 Size: 7423 AleI CACnn'nnGTG Cuts at: 1166 2817 1166 Size: 1651 5772 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4318 1184 Size: 3134 4289 AlwNI CAG_nnn'CTG Cuts at: 3034 3043 3506 4330 5166 6738 3034 Size: 9 463 824 836 1572 3719 Fragments arranged by size: 3719 1572 836 824 463 9 ApaI G_GGCC'C Cuts at: 2145 2145 Size: 7423 ApaLI G'TGCA_C Cuts at: 5480 6636 5480 Size: 1156 6267 ApoI r'AATT_y Cuts at: 1493 2215 4732 4958 5110 7219 1493 Size: 722 2517 226 152 2109 1697 Fragments arranged by size: 2517 2109 1697 722 226 152 AseI AT'TA_AT Cuts at: 160 1032 3399 5977 160 Size: 872 2367 2578 1606 Fragments arranged by size: 2578 2367 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7423 AvaI C'yCGr_G Cuts at: 1870 1943 2388 3654 4011 4194 4748 1870 Size: 73 445 1266 357 183 554 4545 Fragments arranged by size: 4545 1266 554 445 357 183 73 BamHI G'GATC_C Cuts at: 2044 4262 4753 5120 2044 Size: 2218 491 367 4347 Fragments arranged by size: 4347 2218 491 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4591 4744 6199 618 Size: 325 217 627 2804 153 1455 1842 Fragments arranged by size: 2804 1842 1455 627 325 217 153 BanII G_rGCy'C Cuts at: 1391 2145 4742 1391 Size: 754 2597 4072 Fragments arranged by size: 4072 2597 754 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3131 3987 928 Size: 971 1232 856 4364 Fragments arranged by size: 4364 1232 971 856 BbvCI CC'TCA_GC Cuts at: 3797 4254 4694 3797 Size: 457 440 6526 Fragments arranged by size: 6526 457 440 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5614 5648 1903 Size: 34 3677 34 3678 Fragments arranged by size: 3678 3677 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5315 6525 5315 Size: 1210 6213 BclI T'GATC_A Cuts at: 1548 2617 1548 Size: 1069 6354 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2717 2960 3311 Size: 107 122 71 631 605 1045 243 351 Cuts at: 3311 6034 136 Size: 2723 1525 Fragments arranged by size: 2723 1525 1045 631 605 351 243 122 107 71 BglII A'GATC_T Cuts at: 7418 7418 Size: 7423 BlpI GC'TnA_GC Cuts at: 4026 4840 4026 Size: 814 6609 Bme1580I G_kGCm'C Cuts at: 1790 2145 4012 5484 6640 1790 Size: 355 1867 1472 1156 2573 Fragments arranged by size: 2573 1867 1472 1156 355 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7423 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5154 6112 442 Size: 416 53 83 399 24 3737 958 1753 Fragments arranged by size: 3737 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7423 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2414 2414 Size: 7423 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2939 6083 1239 Size: 1700 3144 2579 Fragments arranged by size: 3144 2579 1700 Bpu10I CC'TnA_GC Cuts at: 3797 4254 4694 3797 Size: 457 440 6526 Fragments arranged by size: 6526 457 440 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2246 2905 5548 6413 6711 2246 Size: 659 2643 865 298 2958 Fragments arranged by size: 2958 2643 865 659 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6086 882 Size: 617 4587 2219 Fragments arranged by size: 4587 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3667 4675 5214 493 Size: 711 2463 1008 539 2702 Fragments arranged by size: 2702 2463 1008 711 539 BsaBI GATnn'nnATC Cuts at: 7417 7417 Size: 7423 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5230 5612 6247 275 Size: 53 83 186 936 3697 382 635 1451 Fragments arranged by size: 3697 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5856 6254 6528 6675 7086 5856 Size: 398 274 147 411 6193 Fragments arranged by size: 6193 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2240 2270 1178 Size: 30 165 30 837 30 6331 Fragments arranged by size: 6331 837 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2282 2838 2841 2844 2912 2936 3926 1186 Size: 1096 556 3 3 68 24 990 4683 Fragments arranged by size: 4683 1096 990 556 68 24 3 3 BseYI C'CCAG_C Cuts at: 3813 6626 7122 3813 Size: 2813 496 4114 Fragments arranged by size: 4114 2813 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2959 3390 4424 4514 4706 1163 Size: 573 1223 431 1034 90 192 3880 Fragments arranged by size: 3880 1223 1034 573 431 192 90 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4873 5634 6662 1540 Size: 275 186 2872 761 1028 2301 Fragments arranged by size: 2872 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5484 5569 6640 5484 Size: 85 1071 6267 Fragments arranged by size: 6267 1071 85 BsmI GAATG_Cn' Cuts at: 2506 4489 4934 5027 2506 Size: 1983 445 93 4902 Fragments arranged by size: 4902 1983 445 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2145 4012 4742 5484 5569 6640 1391 Size: 399 355 1867 730 742 85 1071 2174 Fragments arranged by size: 2174 1867 1071 742 730 399 355 85 BspEI T'CCGG_A Cuts at: 7086 7086 Size: 7423 BspHI T'CATG_A Cuts at: 5312 7042 5312 Size: 1730 5693 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3051 4338 4350 4778 844 Size: 2207 1287 12 428 3489 Fragments arranged by size: 3489 2207 1287 428 12 BsrBI CCG'CTC Cuts at: 1394 1823 5310 1394 Size: 429 3487 3507 Fragments arranged by size: 3507 3487 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2515 3070 3585 4138 4290 4300 5918 Size: 1183 1267 555 515 553 152 10 1618 Cuts at: 5918 6092 65 Size: 174 1396 Fragments arranged by size: 1618 1396 1267 1183 555 553 515 174 152 10 BsrFI r'CCGG_y Cuts at: 1700 1810 6067 6254 1700 Size: 110 4257 187 2869 Fragments arranged by size: 4257 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7423 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7423 BssSI C'ACGA_G Cuts at: 5483 6495 5483 Size: 1012 6411 BstAPI GCAn_nnn'nTGC Cuts at: 794 3043 4330 794 Size: 2249 1287 3887 Fragments arranged by size: 3887 2249 1287 BstBI TT'CG_AA Cuts at: 2296 3355 6227 2296 Size: 1059 2872 3492 Fragments arranged by size: 3492 2872 1059 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7423 BstYI r'GATC_y Cuts at: 1972 2044 3457 4262 4753 5120 5505 5522 6974 Size: 72 1413 805 491 367 385 17 1452 Cuts at: 6974 7060 7072 7418 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1413 805 491 385 367 346 86 72 17 12 Bsu36I CC'TnA_GG Cuts at: 3390 3909 3390 Size: 519 6904 BtgI C'CryG_G Cuts at: 513 1065 3786 3819 513 Size: 552 2721 33 4117 Fragments arranged by size: 4117 2721 552 33 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4336 487 Size: 683 56 3110 3574 Fragments arranged by size: 3574 3110 683 56 BtsI GCAGTG_nn' Cuts at: 2787 3894 4939 5733 5753 2787 Size: 1107 1045 794 20 4457 Fragments arranged by size: 4457 1107 1045 794 20 ClaI AT'CG_AT Cuts at: 1554 3840 1554 Size: 2286 5137 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7388 DraI TTT'AAA Cuts at: 3466 4727 3466 Size: 1261 6162 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6430 809 Size: 507 5114 1802 Fragments arranged by size: 5114 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2608 2961 4000 Size: 54 1425 50 275 18 778 353 1039 Cuts at: 4000 4068 4149 4282 4870 5759 7388 8 Size: 68 81 133 588 889 1629 43 Fragments arranged by size: 1629 1425 1039 889 778 588 353 275 133 81 68 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4870 1537 Size: 275 3058 4090 Fragments arranged by size: 4090 3058 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2150 2359 4610 5353 1353 Size: 797 209 2251 743 3423 Fragments arranged by size: 3423 2251 797 743 209 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3014 6010 6384 6530 387 Size: 1064 138 1425 2996 374 146 1280 Fragments arranged by size: 2996 1425 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2571 2875 2939 4125 5486 6083 6870 Size: 393 939 304 64 1186 1361 597 787 Cuts at: 6870 7138 1239 Size: 268 1524 Fragments arranged by size: 1524 1361 1186 939 787 597 393 304 268 64 EcoNI CCTnn'n_nnAGG Cuts at: 4460 4460 Size: 7423 EcoO109I rG'GnC_Cy Cuts at: 2141 2142 2728 3031 3040 3903 4327 4690 2141 Size: 1 586 303 9 863 424 363 4874 Fragments arranged by size: 4874 863 586 424 363 303 9 1 EcoRI G'AATT_C Cuts at: 4732 5110 4732 Size: 378 7045 FspI TGC'GCA Cuts at: 5929 5929 Size: 7423 HaeII r_GCGC'y Cuts at: 1678 2905 4449 6570 1678 Size: 1227 1544 2121 2531 Fragments arranged by size: 2531 2121 1544 1227 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2053 2085 2336 2368 2789 Size: 32 135 32 481 32 251 32 421 Cuts at: 2789 2821 4009 4041 4247 4279 6064 6096 6138 Size: 32 1188 32 206 32 1785 32 42 Cuts at: 6138 6170 1373 Size: 32 2626 Fragments arranged by size: 2626 1785 1188 481 421 251 206 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4767 5013 669 Size: 988 281 2829 246 3079 Fragments arranged by size: 3079 2829 988 281 246 HindIII A'AGCT_T Cuts at: 748 3347 4277 748 Size: 2599 930 3894 Fragments arranged by size: 3894 2599 930 HpaI GTT'AAC Cuts at: 5013 5013 Size: 7423 KpnI G_GTAC'C Cuts at: 4748 4748 Size: 7423 MfeI C'AATT_G Cuts at: 5022 5022 Size: 7423 MluI A'CGCG_T Cuts at: 2978 7299 2978 Size: 4321 3102 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2070 3768 3897 6537 6721 1305 Size: 765 1698 129 2640 184 2007 Fragments arranged by size: 2640 2007 1698 765 184 129 MscI TGG'CCA Cuts at: 10 64 1489 1832 2610 4002 4070 4151 10 Size: 54 1425 343 778 1392 68 81 3282 Fragments arranged by size: 3282 1425 1392 778 343 81 68 54 MslI CAynn'nnrTG Cuts at: 518 1166 2700 2817 3752 4142 4169 5381 5740 Size: 648 1534 117 935 390 27 1212 359 Cuts at: 5740 5899 518 Size: 159 2042 Fragments arranged by size: 2042 1534 1212 935 648 390 359 159 117 27 MspA1I CmG'CkG Cuts at: 1606 2464 2746 3629 3991 4372 4839 5516 6664 Size: 858 282 883 362 381 467 677 1148 Cuts at: 6664 6909 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 883 858 677 467 381 362 282 245 NcoI C'CATG_G Cuts at: 513 1065 3819 513 Size: 552 2754 4117 Fragments arranged by size: 4117 2754 552 NdeI CA'TA_TG Cuts at: 387 2842 387 Size: 2455 4968 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7423 NotI GC'GGCC_GC Cuts at: 4870 4870 Size: 7423 NspI r_CATG'y Cuts at: 1132 1229 2688 3284 4781 6326 1132 Size: 97 1459 596 1497 1545 2229 Fragments arranged by size: 2229 1545 1497 1459 596 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7423 PciI A'CATG_T Cuts at: 6322 6322 Size: 7423 PflMI CCAn_nnn'nTGG Cuts at: 1108 2185 3820 1108 Size: 1077 1635 4711 Fragments arranged by size: 4711 1635 1077 PfoI T'CCnGG_A Cuts at: 3186 3870 4077 4265 3186 Size: 684 207 188 6344 Fragments arranged by size: 6344 684 207 188 PmeI GTTT'AAAC Cuts at: 4727 4727 Size: 7423 PmlI CAC'GTG Cuts at: 4675 4675 Size: 7423 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2149 2181 5492 5524 7005 7037 2149 Size: 32 3311 32 1481 32 2535 Fragments arranged by size: 3311 2535 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2728 3040 3903 4327 2728 Size: 312 863 424 5824 Fragments arranged by size: 5824 863 424 312 PshAI GACnn'nnGTC Cuts at: 1613 2012 3968 1613 Size: 399 1956 5068 Fragments arranged by size: 5068 1956 399 PsiI TTA'TAA Cuts at: 4993 4993 Size: 7423 PspOMI G'GGCC_C Cuts at: 2141 2141 Size: 7423 PspXI vC'TCGA_Gb Cuts at: 2388 2388 Size: 7423 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2277 2309 3336 3368 2277 Size: 32 1027 32 6332 Fragments arranged by size: 6332 1027 32 32 PstI C_TGCA'G Cuts at: 830 2329 2632 2649 2751 3039 4385 4775 830 Size: 1499 303 17 102 288 1346 390 3478 Fragments arranged by size: 3478 1499 1346 390 303 288 102 17 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7423 PvuII CAG'CTG Cuts at: 2464 2746 3629 3991 4372 2464 Size: 282 883 362 381 5515 Fragments arranged by size: 5515 883 381 362 282 SalI G'TCGA_C Cuts at: 1936 4765 1936 Size: 2829 4594 SapI GCTCTTCn'nnn_ Cuts at: 2359 2359 Size: 7423 SbfI CC_TGCA'GG Cuts at: 3039 4775 3039 Size: 1736 5687 ScaI AGT'ACT Cuts at: 5671 7383 5671 Size: 1712 5711 SfcI C'TryA_G Cuts at: 826 1045 2283 2325 2628 2645 2690 2747 3035 Size: 219 1238 42 303 17 45 57 288 Cuts at: 3035 4381 4771 5906 6587 6778 826 Size: 1346 390 1135 681 191 1471 Fragments arranged by size: 1471 1346 1238 1135 681 390 303 288 219 191 57 45 42 17 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7423 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7423 SmaI CCC'GGG Cuts at: 1872 3656 4013 4750 1872 Size: 1784 357 737 4545 Fragments arranged by size: 4545 1784 737 357 SmlI C'TyrA_G Cuts at: 820 1017 1943 2261 2388 2884 5527 6428 6690 Size: 197 926 318 127 496 2643 901 262 Cuts at: 6690 820 Size: 1553 Fragments arranged by size: 2643 1553 926 901 496 318 262 197 127 SnaBI TAC'GTA Cuts at: 493 5214 493 Size: 4721 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7423 SphI G_CATG'C Cuts at: 1132 2688 4781 1132 Size: 1556 2093 3774 Fragments arranged by size: 3774 2093 1556 SrfI GCCC'GGGC Cuts at: 4013 4013 Size: 7423 SspI AAT'ATT Cuts at: 5 52 5347 5 Size: 47 5295 2081 Fragments arranged by size: 5295 2081 47 StuI AGG'CCT Cuts at: 1517 2911 1517 Size: 1394 6029 StyI C'CwwG_G Cuts at: 513 1065 3819 4862 513 Size: 552 2754 1043 3074 Fragments arranged by size: 3074 2754 1043 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5450 5467 5620 5805 6250 325 Size: 1255 3870 17 153 185 445 1498 Fragments arranged by size: 3870 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3054 5669 7381 Size: 274 80 33 51 1443 1077 2615 1712 Cuts at: 7381 96 Size: 138 Fragments arranged by size: 2615 1712 1443 1077 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7383 7415 7383 Size: 32 7391 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7423 XbaI T'CTAG_A Cuts at: 2056 4759 2056 Size: 2703 4720 XhoI C'TCGA_G Cuts at: 1943 2388 1943 Size: 445 6978 XmaI C'CCGG_G Cuts at: 1870 3654 4011 4748 1870 Size: 1784 357 737 4545 Fragments arranged by size: 4545 1784 737 357 XmnI GAAnn'nnTTC Cuts at: 2292 5552 2292 Size: 3260 4163 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5231 276 Size: 53 83 186 936 3697 2468 Fragments arranged by size: 3697 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI RsrII SacI SacII SanDI SexAI SfiI SfoI SwaI XcmI