Restriction map for
pFN21AB5697
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# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII AccIII AclI AflII AflIII
AhdI Alw44I Aor51HI ApaLI AscI
AseI AsnI AspEI AspI AviII
AvrII BbeI BbvCI BcgI BfrI
BlnI BsaBI BseAI BsiWI BsmBI
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrDI BsrGI BstAPI BstBI BstEII
BstPI ClaI CpoI DraI DraIII
Eam1105I Eco47III EcoO65I EcoRI EcoRV
EcoT22I FauI FseI FspI HincII
HindII HindIII HpaI KasI KspI
MamI MfeI MluI MroI MunI
NarI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PmeI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SexAI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI SphI SspBI SspI
StuI SwaI Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 63 enzymes) #
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Acc65I 1652 GGTACC G^GTACC
AccI 1567 GTATAC GT^[AC][GT]AC
AcsI 1759 AAATTC [GA]^AATT[CT]
AcyI 1491 GACGCC G[GA]^CG[CT]C
AgeI 1469 ACCGGT A^CCGGT
AlwNI 590 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 39 CCTGAGG CC^T[ACGT]AGG
ApoI 1759 AAATTC [GA]^AATT[CT]
Asp700I 162 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1652 GGTACC G^GTACC
AvaI 17 CCCGGG C^[CT]CG[GA]G
BamHI 1794 GGATCC G^GATCC
BbrPI 1496 CACGTG CAC^GTG
BciVI 541 GGATAC GTATCC(6/5)
BclI 509 TGATCA T^GATCA
BfaI 1972 CTAG C^TAG
BlpI 69 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 69 GCTGAGC GC^T[ACGT]AGC
BsaAI 1496 CACGTG [CT]AC^GT[GA]
BsaHI 1491 GACGCC G[GA]^CG[CT]C
BsaWI 1469 ACCGGT [AT]^CCGG[AT]
BseRI 1585 GAGGAG GAGGAG(10/8)
BsoBI 17 CCCGGG C^[CT]CG[GA]G
BspHI 506 TCATGA T^CATGA
BspMI 1865 ACCTGC ACCTGC(4/8)
BssHII 23 GCGCGC G^CGCGC
Bst1107I 1567 GTATAC GTA^TAC
BstZ17I 1567 GTATAC GTA^TAC
Bsu36I 39 CCTGAGG CC^T[ACGT]AGG
CelII 69 GCTGAGC GC^T[ACGT]AGC
DrdI 1002 GACCGCCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1935 GAAGAG CTCTTC(1/4)
Eco81I 39 CCTGAGG CC^T[ACGT]AGG
EcoNI 1171 CCTGACCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 509 TGATCA T^GATCA
HgaI 1491 GACGC GACGC(5/10)
Hin1I 1491 GACGCC G[GA]^CG[CT]C
KpnI 1652 GGTACC GGTAC^C
Ksp632I 1935 GAAGAG CTCTTC(1/4)
MaeI 1972 CTAG C^TAG
MaeII 1497 ACGT A^CGT
MseI 1715 TTAA T^TAA
NaeI 1096 GCCGGC GCC^GGC
NgoMIV 1096 GCCGGC G^CCGGC
PinAI 1469 ACCGGT A^CCGGT
PmaCI 1496 CACGTG CAC^GTG
PmlI 1496 CACGTG CAC^GTG
PpuMI 33 GGGTCCC [GA]G^G[AT]CC[CT]
PstI 1867 CTGCAG CTGCA^G
RcaI 506 TCATGA T^CATGA
SapI 1935 GAAGAGC GCTCTTC(1/4)
SbfI 1866 CCTGCAGG CCTGCA^GG
ScaI 692 AGTACT AGT^ACT
SfcI 1867 CTGCAG C^T[GA][CT]AG
SmaI 17 CCCGGG CCC^GGG
Sse8387I 1866 CCTGCAGG CCTGCA^GG
TfiI 482 GAATC G^A[AT]TC
Tru9I 1715 TTAA T^TAA
Tsp509I 1760 AATT ^AATT
XcmI 1370 CCACCCCCACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 17 CCCGGG C^CCGGG
XmaI 17 CCCGGG C^CCGGG
XmnI 162 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
BbsI 1734 GAAGAC GAAGAC(2/6)
1048 GTCTTC
BglI 1255 GCCCTGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1506 GCCCATGGGGC
BglII 1400 AGATCT A^GATCT
1451 AGATCT
BpuAI 1734 GAAGAC GAAGAC(2/6)
1048 GTCTTC
BsaI 283 GAGACC GGTCTC(1/5)
1000 GAGACC
BsmI 1830 GAATGC GAATGC(1/-1)
1319 GCATTC
EagI 750 CGGCCG C^GGCCG
1098 CGGCCG
EclXI 750 CGGCCG C^GGCCG
1098 CGGCCG
Eco52I 750 CGGCCG C^GGCCG
1098 CGGCCG
MslI 532 CATGGCGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
616 CATTTTGATG
PleI 566 GACTC GAGTC(4/5)
610 GACTC
PvuII 735 CAGCTG CAG^CTG
909 CAGCTG
SacI 1327 GAGCTC GAGCT^C
1543 GAGCTC
TaqI 281 TCGA T^CGA
480 TCGA
TthHB8I 281 TCGA T^CGA
480 TCGA
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# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
ApaI AspHI AvaII BalI BanI
BanII BbvI BcnI BmyI BpmI
BsaJI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrFI
BsrI BssKI BssSI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeIII MboI MboII MflI
MluNI MnlI MscI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV PflMI RsaI
Sau3AI Sau96I ScrFI SfaNI StyI
TseI Tsp45I TspRI Van91I XhoII