(Circular) (Six-base) MAPSORT of: pFN21AB5697.seq Check: 8753 from: 1 to: 6685 pFN21AB5697 6685 bp With 182 enzymes: SgfI * October 20, 2009 10:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4495 278 Size: 53 83 186 936 2959 2468 Fragments arranged by size: 2959 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3573 4028 1937 Size: 1636 455 4594 Fragments arranged by size: 4594 1636 455 Acc65I G'GTAC_C Cuts at: 3657 4006 3657 Size: 349 6336 AclI AA'CG_TT Cuts at: 1563 4812 5185 1563 Size: 3249 373 3063 Fragments arranged by size: 3249 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2234 2883 3938 3959 4748 6132 6400 1632 Size: 602 649 1055 21 789 1384 268 1917 Fragments arranged by size: 1917 1384 1055 789 649 602 268 21 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6488 AflIII A'CryG_T Cuts at: 1203 5584 6561 1203 Size: 4381 977 1327 Fragments arranged by size: 4381 1327 977 AgeI A'CCGG_T Cuts at: 3474 5516 3474 Size: 2042 4643 AhdI GACnn_n'nnGTC Cuts at: 5414 5414 Size: 6685 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6685 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6685 AlwNI CAG_nnn'CTG Cuts at: 2600 4428 6000 2600 Size: 1828 1572 3285 Fragments arranged by size: 3285 1828 1572 ApaI G_GGCC'C Cuts at: 3262 3392 3522 3262 Size: 130 130 6425 Fragments arranged by size: 6425 130 130 ApaLI G'TGCA_C Cuts at: 4742 5898 4742 Size: 1156 5529 ApoI r'AATT_y Cuts at: 1493 3764 3994 4220 4372 6481 1493 Size: 2271 230 226 152 2109 1697 Fragments arranged by size: 2271 2109 1697 230 226 152 AseI AT'TA_AT Cuts at: 160 1032 5239 160 Size: 872 4207 1606 Fragments arranged by size: 4207 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6685 AvaI C'yCGr_G Cuts at: 1870 1943 2022 4010 1870 Size: 73 79 1988 4545 Fragments arranged by size: 4545 1988 79 73 BamHI G'GATC_C Cuts at: 3799 4015 4382 3799 Size: 216 367 6102 Fragments arranged by size: 6102 367 216 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2957 3041 3268 3365 3657 Size: 325 217 627 1170 84 227 97 292 Cuts at: 3657 3905 4006 5461 618 Size: 248 101 1455 1842 Fragments arranged by size: 1842 1455 1170 627 325 292 248 227 217 101 97 84 BanII G_rGCy'C Cuts at: 1391 3262 3336 3392 3522 3552 4004 1391 Size: 1871 74 56 130 30 452 4072 Fragments arranged by size: 4072 1871 452 130 74 56 30 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3046 3746 928 Size: 971 1147 700 3867 Fragments arranged by size: 3867 1147 971 700 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4876 4910 1903 Size: 34 2939 34 3678 Fragments arranged by size: 3678 2939 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2540 4577 5787 2540 Size: 2037 1210 3438 Fragments arranged by size: 3438 2037 1210 BclI T'GATC_A Cuts at: 1548 2514 1548 Size: 966 5719 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 3266 3517 Size: 107 122 71 631 605 337 1257 251 Cuts at: 3517 5296 136 Size: 1779 1525 Fragments arranged by size: 1779 1525 1257 631 605 337 251 122 107 71 BglII A'GATC_T Cuts at: 3405 3456 6680 3405 Size: 51 3224 3410 Fragments arranged by size: 3410 3224 51 BlpI GC'TnA_GC Cuts at: 2075 4102 2075 Size: 2027 4658 Bme1580I G_kGCm'C Cuts at: 1790 2903 3046 3262 3392 3522 4746 5902 1790 Size: 1113 143 216 130 130 1224 1156 2573 Fragments arranged by size: 2573 1224 1156 1113 216 143 130 130 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6685 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4416 5374 442 Size: 416 53 83 399 24 2999 958 1753 Fragments arranged by size: 2999 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6685 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2476 3257 3668 3745 3777 3790 3944 5345 Size: 1237 781 411 77 32 13 154 1401 Cuts at: 5345 1239 Size: 2579 Fragments arranged by size: 2579 1401 1237 781 411 154 77 32 13 Bpu10I CC'TnA_GC Cuts at: 3546 3546 Size: 6685 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4810 5675 5973 4810 Size: 865 298 5522 Fragments arranged by size: 5522 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2282 2999 5348 882 Size: 617 783 717 2349 2219 Fragments arranged by size: 2349 2219 783 717 617 BsaAI yAC'GTr Cuts at: 493 1204 3503 4476 493 Size: 711 2299 973 2702 Fragments arranged by size: 2702 2299 973 711 BsaBI GATnn'nnATC Cuts at: 6679 6679 Size: 6685 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3497 4492 4874 5509 Size: 53 83 186 936 1964 995 382 635 Cuts at: 5509 275 Size: 1451 Fragments arranged by size: 1964 1451 995 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3474 5118 5516 5790 5937 6348 3474 Size: 1644 398 274 147 411 3811 Fragments arranged by size: 3811 1644 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3641 3671 1178 Size: 30 165 30 2238 30 4192 Fragments arranged by size: 4192 2238 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3605 1186 Size: 2419 4266 BseYI C'CCAG_C Cuts at: 2529 3385 5888 6384 2529 Size: 856 2503 496 2830 Fragments arranged by size: 2830 2503 856 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2728 2902 3076 1163 Size: 573 992 174 174 4772 Fragments arranged by size: 4772 992 573 174 174 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2758 2977 3106 3157 4135 4896 Size: 275 186 757 219 129 51 978 761 Cuts at: 4896 5924 1540 Size: 1028 2301 Fragments arranged by size: 2301 1028 978 761 757 275 219 186 129 51 BsiHKAI G_wGCw'C Cuts at: 2783 3243 3336 3552 4746 4831 5902 2783 Size: 460 93 216 1194 85 1071 3566 Fragments arranged by size: 3566 1194 1071 460 216 93 85 BsmI GAATG_Cn' Cuts at: 3324 3841 4196 4289 3324 Size: 517 355 93 5720 Fragments arranged by size: 5720 517 355 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2783 2903 3046 3243 3262 3336 3392 Size: 399 993 120 143 197 19 74 56 Cuts at: 3392 3522 3552 4004 4746 4831 5902 1391 Size: 130 30 452 742 85 1071 2174 Fragments arranged by size: 2174 1071 993 742 452 399 197 143 130 120 85 74 56 30 19 BspEI T'CCGG_A Cuts at: 6348 6348 Size: 6685 BspHI T'CATG_A Cuts at: 2511 4574 6304 2511 Size: 2063 1730 2892 Fragments arranged by size: 2892 2063 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3879 4040 844 Size: 3035 161 3489 Fragments arranged by size: 3489 3035 161 BsrBI CCG'CTC Cuts at: 1394 1823 4572 1394 Size: 429 2749 3507 Fragments arranged by size: 3507 2749 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5180 5354 65 Size: 1183 3932 174 1396 Fragments arranged by size: 3932 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2753 3101 3368 3474 5329 5516 1700 Size: 110 943 348 267 106 1855 187 2869 Fragments arranged by size: 2869 1855 943 348 267 187 110 106 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6685 BssHII G'CGCG_C Cuts at: 1925 2028 1925 Size: 103 6582 BssSI C'ACGA_G Cuts at: 2439 3200 3212 4745 5757 2439 Size: 761 12 1533 1012 3367 Fragments arranged by size: 3367 1533 1012 761 12 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6685 BstBI TT'CG_AA Cuts at: 5489 5489 Size: 6685 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6685 BstXI CCAn_nnnn'nTGG Cuts at: 3218 3235 3254 3646 3218 Size: 17 19 392 6257 Fragments arranged by size: 6257 392 19 17 BstYI r'GATC_y Cuts at: 1972 3405 3444 3456 3627 3799 4015 4382 4767 Size: 1433 39 12 171 172 216 367 385 Cuts at: 4767 4784 6236 6322 6334 6680 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1433 385 367 346 216 172 171 86 39 17 12 12 BstZ17I GTA'TAC Cuts at: 3574 3574 Size: 6685 Bsu36I CC'TnA_GG Cuts at: 2045 2045 Size: 6685 BtgI C'CryG_G Cuts at: 513 1065 2004 2847 3228 3448 3513 513 Size: 552 939 843 381 220 65 3685 Fragments arranged by size: 3685 939 843 552 381 220 65 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2136 2475 2556 2778 3421 3805 Size: 683 56 910 339 81 222 643 384 Cuts at: 3805 487 Size: 3367 Fragments arranged by size: 3367 910 683 643 384 339 222 81 56 BtsI GCAGTG_nn' Cuts at: 2160 2471 3115 4201 4995 5015 2160 Size: 311 644 1086 794 20 3830 Fragments arranged by size: 3830 1086 794 644 311 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6685 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6650 DraI TTT'AAA Cuts at: 3989 3989 Size: 6685 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3013 5692 809 Size: 507 1697 2679 1802 Fragments arranged by size: 2679 1802 1697 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2325 2524 2755 Size: 54 1425 50 275 18 495 199 231 Cuts at: 2755 3103 3220 3531 4132 5021 6650 8 Size: 348 117 311 601 889 1629 43 Fragments arranged by size: 1629 1425 889 601 495 348 311 275 231 199 117 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2755 3103 4132 1537 Size: 275 943 348 1029 4090 Fragments arranged by size: 4090 1029 943 348 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3935 4615 1353 Size: 2582 680 3423 Fragments arranged by size: 3423 2582 680 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3502 5272 5646 5792 387 Size: 1064 138 1913 1770 374 146 1280 Fragments arranged by size: 1913 1770 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3334 3550 3334 Size: 216 6469 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2234 2476 2883 3257 3668 3745 3777 Size: 393 602 242 407 374 411 77 32 Cuts at: 3777 3790 3938 3944 3959 4748 5345 6132 6400 Size: 13 148 6 15 789 597 787 268 Cuts at: 6400 1239 Size: 1524 Fragments arranged by size: 1524 789 787 602 597 411 407 393 374 268 242 148 77 32 15 13 6 EcoNI CCTnn'n_nnAGG Cuts at: 3180 3180 Size: 6685 EcoO109I rG'GnC_Cy Cuts at: 2039 3258 3259 3342 3389 3518 3519 3580 2039 Size: 1219 1 83 47 129 1 61 5144 Fragments arranged by size: 5144 1219 129 83 61 47 1 1 EcoRI G'AATT_C Cuts at: 3994 4372 3994 Size: 378 6307 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2728 2836 3010 3184 2728 Size: 108 174 174 6229 Fragments arranged by size: 6229 174 174 108 FspI TGC'GCA Cuts at: 5191 5191 Size: 6685 HaeII r_GCGC'y Cuts at: 1678 2015 2054 3102 5832 1678 Size: 337 39 1048 2730 2531 Fragments arranged by size: 2730 2531 1048 337 39 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2251 2283 5326 5358 5400 Size: 32 135 32 679 32 3043 32 42 Cuts at: 5400 5432 1373 Size: 32 2626 Fragments arranged by size: 3043 2626 679 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4029 4275 669 Size: 988 281 2091 246 3079 Fragments arranged by size: 3079 2091 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6685 HpaI GTT'AAC Cuts at: 4275 4275 Size: 6685 KpnI G_GTAC'C Cuts at: 3661 4010 3661 Size: 349 6336 MfeI C'AATT_G Cuts at: 4284 4284 Size: 6685 MluI A'CGCG_T Cuts at: 6561 6561 Size: 6685 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3993 5799 5983 1305 Size: 2688 1806 184 2007 Fragments arranged by size: 2688 2007 1806 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2327 3222 3533 10 Size: 54 1425 343 495 895 311 3162 Fragments arranged by size: 3162 1425 895 495 343 311 54 MslI CAynn'nnrTG Cuts at: 518 1166 2541 2625 4643 5002 5161 518 Size: 648 1375 84 2018 359 159 2042 Fragments arranged by size: 2042 2018 1375 648 359 159 84 MspA1I CmG'CkG Cuts at: 1606 2529 2742 2916 2949 4101 4778 5926 6171 Size: 923 213 174 33 1152 677 1148 245 Cuts at: 6171 1606 Size: 2120 Fragments arranged by size: 2120 1152 1148 923 677 245 213 174 33 NaeI GCC'GGC Cuts at: 3103 3103 Size: 6685 NcoI C'CATG_G Cuts at: 513 1065 2004 2847 3228 3513 513 Size: 552 939 843 381 285 3685 Fragments arranged by size: 3685 939 843 552 381 285 NdeI CA'TA_TG Cuts at: 387 387 Size: 6685 NgoMIV G'CCGG_C Cuts at: 3101 3101 Size: 6685 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6685 NotI GC'GGCC_GC Cuts at: 4132 4132 Size: 6685 NspI r_CATG'y Cuts at: 1132 1229 4043 5588 1132 Size: 97 2814 1545 2229 Fragments arranged by size: 2814 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3254 1348 Size: 1906 4779 PciI A'CATG_T Cuts at: 5584 5584 Size: 6685 PflMI CCAn_nnn'nTGG Cuts at: 1108 2600 3229 3540 3706 1108 Size: 1492 629 311 166 4087 Fragments arranged by size: 4087 1492 629 311 166 PfoI T'CCnGG_A Cuts at: 3552 3552 Size: 6685 PmeI GTTT'AAAC Cuts at: 3989 3989 Size: 6685 PmlI CAC'GTG Cuts at: 3503 3503 Size: 6685 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4754 4786 6267 6299 4754 Size: 32 1481 32 5140 Fragments arranged by size: 5140 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2039 2039 Size: 6685 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6685 PsiI TTA'TAA Cuts at: 4255 4255 Size: 6685 PspOMI G'GGCC_C Cuts at: 3258 3388 3518 3258 Size: 130 130 6425 Fragments arranged by size: 6425 130 130 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2690 2722 3179 3211 2690 Size: 32 457 32 6164 Fragments arranged by size: 6164 457 32 32 PstI C_TGCA'G Cuts at: 830 3876 4037 830 Size: 3046 161 3478 Fragments arranged by size: 3478 3046 161 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6685 PvuII CAG'CTG Cuts at: 2742 2916 2742 Size: 174 6511 SacI G_AGCT'C Cuts at: 3336 3552 3336 Size: 216 6469 SalI G'TCGA_C Cuts at: 1936 4027 1936 Size: 2091 4594 SanDI GG'GwC_CC Cuts at: 2039 2039 Size: 6685 SapI GCTCTTCn'nnn_ Cuts at: 3935 3935 Size: 6685 SbfI CC_TGCA'GG Cuts at: 3876 4037 3876 Size: 161 6524 ScaI AGT'ACT Cuts at: 2699 4933 6645 2699 Size: 2234 1712 2739 Fragments arranged by size: 2739 2234 1712 SfcI C'TryA_G Cuts at: 826 1045 3872 4033 5168 5849 6040 826 Size: 219 2827 161 1135 681 191 1471 Fragments arranged by size: 2827 1471 1135 681 219 191 161 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6685 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6685 SmaI CCC'GGG Cuts at: 1872 2024 4012 1872 Size: 152 1988 4545 Fragments arranged by size: 4545 1988 152 SmlI C'TyrA_G Cuts at: 820 1017 1943 4789 5690 5952 820 Size: 197 926 2846 901 262 1553 Fragments arranged by size: 2846 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4476 493 Size: 3983 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6685 SphI G_CATG'C Cuts at: 1132 4043 1132 Size: 2911 3774 SspI AAT'ATT Cuts at: 5 52 4609 5 Size: 47 4557 2081 Fragments arranged by size: 4557 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6685 StyI C'CwwG_G Cuts at: 513 1065 2004 2187 2646 2847 3181 3228 3361 Size: 552 939 183 459 201 334 47 133 Cuts at: 3361 3513 4124 513 Size: 152 611 3074 Fragments arranged by size: 3074 939 611 552 459 334 201 183 152 133 47 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3286 4712 4729 4882 5067 5512 325 Size: 1255 1706 1426 17 153 185 445 1498 Fragments arranged by size: 1706 1498 1426 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2697 2733 4931 Size: 274 80 33 51 1443 720 36 2198 Cuts at: 4931 6643 96 Size: 1712 138 Fragments arranged by size: 2198 1712 1443 720 274 138 80 51 36 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6645 6677 6645 Size: 32 6653 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6685 XbaI T'CTAG_A Cuts at: 4021 4021 Size: 6685 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3382 3382 Size: 6685 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6685 XmaI C'CCGG_G Cuts at: 1870 2022 4010 1870 Size: 152 1988 4545 Fragments arranged by size: 4545 1988 152 XmnI GAAnn'nnTTC Cuts at: 2171 4814 2171 Size: 2643 4042 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4493 276 Size: 53 83 186 936 2959 2468 Fragments arranged by size: 2959 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI DraIII EcoRV FseI FspAI KasI NarI NruI NsiI PacI PspXI RsrII SacII SexAI SfiI SfoI SrfI SwaI