Restriction map for
pFN21AB5697
(Linear) (Six-base) MAPSORT of: KIBB5697.seq Check: 5097 from: 1 to: 1980
KIBB5697.orf
With 182 enzymes: SgfI *
February 28, 2007 11:28 ..
AccI GT'mk_AC
Cuts at: 0 1568 1980
Size: 1568 412
Acc65I G'GTAC_C
Cuts at: 0 1652 1980
Size: 1652 328
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 878 1933 1954 1980
Size: 229 649 1055 21 26
Fragments arranged by size:
1055 649 229 26 21
AgeI A'CCGG_T
Cuts at: 0 1469 1980
Size: 1469 511
AlwNI CAG_nnn'CTG
Cuts at: 0 595 1980
Size: 595 1385
ApaI G_GGCC'C
Cuts at: 0 1257 1387 1517 1980
Size: 1257 130 130 463
Fragments arranged by size:
1257 463 130 130
ApoI r'AATT_y
Cuts at: 0 1759 1980
Size: 1759 221
AvaI C'yCGr_G
Cuts at: 0 17 1980
Size: 17 1963
BamHI G'GATC_C
Cuts at: 0 1794 1980
Size: 1794 186
BanI G'GyrC_C
Cuts at: 0 952 1036 1263 1360 1652 1900 1980
Size: 952 84 227 97 292 248 80
Fragments arranged by size:
952 292 248 227 97 84 80
BanII G_rGCy'C
Cuts at: 0 1257 1331 1387 1517 1547 1980
Size: 1257 74 56 130 30 433
Fragments arranged by size:
1257 433 130 74 56 30
BbsI GAAGACnn'nnnn_
Cuts at: 0 1041 1741 1980
Size: 1041 700 239
Fragments arranged by size:
1041 700 239
BciVI GTATCCnnnnn_n'
Cuts at: 0 535 1980
Size: 535 1445
BclI T'GATC_A
Cuts at: 0 509 1980
Size: 509 1471
BglI GCCn_nnn'nGGC
Cuts at: 0 1261 1512 1980
Size: 1261 251 468
Fragments arranged by size:
1261 468 251
BglII A'GATC_T
Cuts at: 0 1400 1451 1980
Size: 1400 51 529
Fragments arranged by size:
1400 529 51
BlpI GC'TnA_GC
Cuts at: 0 70 1980
Size: 70 1910
Bme1580I G_kGCm'C
Cuts at: 0 898 1041 1257 1387 1517 1980
Size: 898 143 216 130 130 463
Fragments arranged by size:
898 463 216 143 130 130
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 471 1252 1663 1740 1772 1785 1939 1980
Size: 471 781 411 77 32 13 154 41
Fragments arranged by size:
781 471 411 154 77 41 32 13
Bpu10I CC'TnA_GC
Cuts at: 0 1541 1980
Size: 1541 439
BsaI GGTCTCn'nnnn_
Cuts at: 0 277 994 1980
Size: 277 717 986
Fragments arranged by size:
986 717 277
BsaAI yAC'GTr
Cuts at: 0 1498 1980
Size: 1498 482
BsaHI Gr'CG_yC
Cuts at: 0 1492 1980
Size: 1492 488
BsaWI w'CCGG_w
Cuts at: 0 1469 1980
Size: 1469 511
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1636 1666 1980
Size: 1636 30 314
Fragments arranged by size:
1636 314 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1600 1980
Size: 1600 380
BseYI C'CCAG_C
Cuts at: 0 524 1380 1980
Size: 524 856 600
Fragments arranged by size:
856 600 524
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 723 897 1071 1980
Size: 723 174 174 909
Fragments arranged by size:
909 723 174 174
BsiEI CG_ry'CG
Cuts at: 0 753 972 1101 1152 1980
Size: 753 219 129 51 828
Fragments arranged by size:
828 753 219 129 51
BsiHKAI G_wGCw'C
Cuts at: 0 778 1238 1331 1547 1980
Size: 778 460 93 216 433
Fragments arranged by size:
778 460 433 216 93
BsmI GAATG_Cn'
Cuts at: 0 1319 1836 1980
Size: 1319 517 144
Fragments arranged by size:
1319 517 144
Bsp1286I G_dGCh'C
Cuts at: 0 778 898 1041 1238 1257 1331 1387 1517
Size: 778 120 143 197 19 74 56 130
Cuts at: 1517 1547 1980
Size: 30 433
Fragments arranged by size:
778 433 197 143 130 120 74 56
30 19
BspHI T'CATG_A
Cuts at: 0 506 1980
Size: 506 1474
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1874 1980
Size: 1874 106
BsrFI r'CCGG_y
Cuts at: 0 748 1096 1363 1469 1980
Size: 748 348 267 106 511
Fragments arranged by size:
748 511 348 267 106
BssHII G'CGCG_C
Cuts at: 0 23 1980
Size: 23 1957
BssSI C'ACGA_G
Cuts at: 0 434 1195 1207 1980
Size: 434 761 12 773
Fragments arranged by size:
773 761 434 12
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1213 1230 1249 1641 1980
Size: 1213 17 19 392 339
Fragments arranged by size:
1213 392 339 19 17
BstYI r'GATC_y
Cuts at: 0 1400 1439 1451 1622 1794 1980
Size: 1400 39 12 171 172 186
Fragments arranged by size:
1400 186 172 171 39 12
BstZ17I GTA'TAC
Cuts at: 0 1569 1980
Size: 1569 411
Bsu36I CC'TnA_GG
Cuts at: 0 40 1980
Size: 40 1940
BtgI C'CryG_G
Cuts at: 0 842 1223 1443 1508 1980
Size: 842 381 220 65 472
Fragments arranged by size:
842 472 381 220 65
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 131 470 551 773 1416 1800 1980
Size: 131 339 81 222 643 384 180
Fragments arranged by size:
643 384 339 222 180 131 81
BtsI GCAGTG_nn'
Cuts at: 0 155 466 1110 1980
Size: 155 311 644 870
Fragments arranged by size:
870 644 311 155
DrdI GACnn_nn'nnGTC
Cuts at: 0 1008 1980
Size: 1008 972
EaeI y'GGCC_r
Cuts at: 0 320 519 750 1098 1215 1526 1980
Size: 320 199 231 348 117 311 454
Fragments arranged by size:
454 348 320 311 231 199 117
EagI C'GGCC_G
Cuts at: 0 750 1098 1980
Size: 750 348 882
Fragments arranged by size:
882 750 348
EarI CTCTTCn'nnn_
Cuts at: 0 1930 1980
Size: 1930 50
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1497 1980
Size: 1497 483
EcoICRI GAG'CTC
Cuts at: 0 1329 1545 1980
Size: 1329 216 435
Fragments arranged by size:
1329 435 216
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 471 878 1252 1663 1740 1772 1785
Size: 229 242 407 374 411 77 32 13
Cuts at: 1785 1933 1939 1954 1980
Size: 148 6 15 26
Fragments arranged by size:
411 407 374 242 229 148 77 32
26 15 13 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1175 1980
Size: 1175 805
EcoO109I rG'GnC_Cy
Cuts at: 0 34 1253 1254 1337 1384 1513 1514 1575
Size: 34 1219 1 83 47 129 1 61
Cuts at: 1575 1980
Size: 405
Fragments arranged by size:
1219 405 129 83 61 47 34 1
1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 723 831 1005 1179 1980
Size: 723 108 174 174 801
Fragments arranged by size:
801 723 174 174 108
HaeII r_GCGC'y
Cuts at: 0 10 49 1097 1980
Size: 10 39 1048 883
Fragments arranged by size:
1048 883 39 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 246 278 1980
Size: 246 32 1702
Fragments arranged by size:
1702 246 32
KpnI G_GTAC'C
Cuts at: 0 1656 1980
Size: 1656 324
MscI TGG'CCA
Cuts at: 0 322 1217 1528 1980
Size: 322 895 311 452
Fragments arranged by size:
895 452 322 311
MslI CAynn'nnrTG
Cuts at: 0 536 620 1980
Size: 536 84 1360
Fragments arranged by size:
1360 536 84
MspA1I CmG'CkG
Cuts at: 0 524 737 911 944 1980
Size: 524 213 174 33 1036
Fragments arranged by size:
1036 524 213 174 33
NaeI GCC'GGC
Cuts at: 0 1098 1980
Size: 1098 882
NcoI C'CATG_G
Cuts at: 0 842 1223 1508 1980
Size: 842 381 285 472
Fragments arranged by size:
842 472 381 285
NgoMIV G'CCGG_C
Cuts at: 0 1096 1980
Size: 1096 884
PasI CC'CwG_GG
Cuts at: 0 1249 1980
Size: 1249 731
PflMI CCAn_nnn'nTGG
Cuts at: 0 595 1224 1535 1701 1980
Size: 595 629 311 166 279
Fragments arranged by size:
629 595 311 279 166
PfoI T'CCnGG_A
Cuts at: 0 1547 1980
Size: 1547 433
PmlI CAC'GTG
Cuts at: 0 1498 1980
Size: 1498 482
PpuMI rG'GwC_Cy
Cuts at: 0 34 1980
Size: 34 1946
PspOMI G'GGCC_C
Cuts at: 0 1253 1383 1513 1980
Size: 1253 130 130 467
Fragments arranged by size:
1253 467 130 130
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 685 717 1174 1206 1980
Size: 685 32 457 32 774
Fragments arranged by size:
774 685 457 32 32
PstI C_TGCA'G
Cuts at: 0 1871 1980
Size: 1871 109
PvuII CAG'CTG
Cuts at: 0 737 911 1980
Size: 737 174 1069
Fragments arranged by size:
1069 737 174
SacI G_AGCT'C
Cuts at: 0 1331 1547 1980
Size: 1331 216 433
Fragments arranged by size:
1331 433 216
SanDI GG'GwC_CC
Cuts at: 0 34 1980
Size: 34 1946
SapI GCTCTTCn'nnn_
Cuts at: 0 1930 1980
Size: 1930 50
SbfI CC_TGCA'GG
Cuts at: 0 1871 1980
Size: 1871 109
ScaI AGT'ACT
Cuts at: 0 694 1980
Size: 694 1286
SfcI C'TryA_G
Cuts at: 0 1867 1980
Size: 1867 113
SmaI CCC'GGG
Cuts at: 0 19 1980
Size: 19 1961
StyI C'CwwG_G
Cuts at: 0 182 641 842 1176 1223 1356 1508 1980
Size: 182 459 201 334 47 133 152 472
Fragments arranged by size:
472 459 334 201 182 152 133 47
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1281 1980
Size: 1281 699
TatI w'GTAC_w
Cuts at: 0 692 728 1980
Size: 692 36 1252
Fragments arranged by size:
1252 692 36
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1377 1980
Size: 1377 603
XmaI C'CCGG_G
Cuts at: 0 17 1980
Size: 17 1963
XmnI GAAnn'nnTTC
Cuts at: 0 166 1980
Size: 166 1814
Enzymes that do cut:
AccI Acc65I AcuI AgeI AlwNI ApaI ApoI AvaI
BamHI BanI BanII BbsI BciVI BclI BglI BglII
BlpI Bme1580I BpmI Bpu10I BsaI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrFI BssHII BssSI BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I
KpnI MscI MslI MspA1I NaeI NcoI NgoMIV PasI
PflMI PfoI PmlI PpuMI PspOMI PsrI PstI PvuII
SacI SanDI SapI SbfI ScaI SfcI SmaI StyI
TaqII TatI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AhdI AleI
AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI
BbeI BbvCI BcgI BfrBI BmgBI BmrI BmtI BplI
BpuEI BsaBI BsiWI BsmBI BspEI BsrBI BsrDI BsrGI
BstAPI BstBI BstEII ClaI CspCI DraI DraIII EcoRI
EcoRV FseI FspI FspAI HincII HindIII HpaI KasI
MfeI MluI MmeI NarI NdeI NheI NotI NruI
NsiI NspI PacI PciI PmeI PpiI PshAI PsiI
PspXI PvuI RsrII SacII SalI SexAI SfiI SfoI
SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI TstI Tth111I XbaI XhoI ZraI