(Circular) (Six-base) MAPSORT of: pFN21AB5682.seq Check: 3488 from: 1 to: 6361 pFN21AB5682 6361 bp With 182 enzymes: SgfI * October 20, 2009 10:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4171 278 Size: 53 83 186 936 2635 2468 Fragments arranged by size: 2635 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2686 3704 1937 Size: 749 1018 4594 Fragments arranged by size: 4594 1018 749 Acc65I G'GTAC_C Cuts at: 3682 3682 Size: 6361 AclI AA'CG_TT Cuts at: 1563 4488 4861 1563 Size: 2925 373 3063 Fragments arranged by size: 3063 2925 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2165 2234 2547 2852 4424 5808 6076 1632 Size: 533 69 313 305 1572 1384 268 1917 Fragments arranged by size: 1917 1572 1384 533 313 305 268 69 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6164 AflIII A'CryG_T Cuts at: 1203 2335 5260 6237 1203 Size: 1132 2925 977 1327 Fragments arranged by size: 2925 1327 1132 977 AgeI A'CCGG_T Cuts at: 5192 5192 Size: 6361 AhdI GACnn_n'nnGTC Cuts at: 2014 5090 2014 Size: 3076 3285 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6361 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2472 3179 1184 Size: 1288 707 4366 Fragments arranged by size: 4366 1288 707 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3264 3296 3264 Size: 32 6329 AlwNI CAG_nnn'CTG Cuts at: 3380 3464 4104 5676 3380 Size: 84 640 1572 4065 Fragments arranged by size: 4065 1572 640 84 ApaI G_GGCC'C Cuts at: 3601 3601 Size: 6361 ApaLI G'TGCA_C Cuts at: 3264 4418 5574 3264 Size: 1154 1156 4051 Fragments arranged by size: 4051 1156 1154 ApoI r'AATT_y Cuts at: 1493 3582 3670 3896 4048 6157 1493 Size: 2089 88 226 152 2109 1697 Fragments arranged by size: 2109 2089 1697 226 152 88 AseI AT'TA_AT Cuts at: 160 1032 4915 160 Size: 872 3883 1606 Fragments arranged by size: 3883 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6361 AvaI C'yCGr_G Cuts at: 1870 1943 3686 1870 Size: 73 1743 4545 Fragments arranged by size: 4545 1743 73 BamHI G'GATC_C Cuts at: 3691 4058 3691 Size: 367 5994 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3204 3682 5137 618 Size: 325 217 627 1417 478 1455 1842 Fragments arranged by size: 1842 1455 1417 627 478 325 217 BanII G_rGCy'C Cuts at: 1391 2203 3601 3680 1391 Size: 812 1398 79 4072 Fragments arranged by size: 4072 1398 812 79 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5390 BbvCI CC'TCA_GC Cuts at: 2736 2736 Size: 6361 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4552 4586 1903 Size: 34 2615 34 3678 Fragments arranged by size: 3678 2615 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3053 4253 5463 3053 Size: 1200 1210 3951 Fragments arranged by size: 3951 1210 1200 BclI T'GATC_A Cuts at: 1548 1548 Size: 6361 BfrBI ATG'CAT Cuts at: 3501 3501 Size: 6361 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4972 136 Size: 107 122 71 631 605 3300 1525 Fragments arranged by size: 3300 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3288 6356 3288 Size: 3068 3293 BlpI GC'TnA_GC Cuts at: 3778 3778 Size: 6361 Bme1580I G_kGCm'C Cuts at: 1790 3268 3601 4422 5578 1790 Size: 1478 333 821 1156 2573 Fragments arranged by size: 2573 1478 1156 821 333 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6361 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2699 4092 5050 Size: 416 53 83 399 24 1282 1393 958 Cuts at: 5050 442 Size: 1753 Fragments arranged by size: 1753 1393 1282 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6361 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2475 2475 Size: 6361 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2320 3096 3291 3426 3587 5021 1239 Size: 1081 776 195 135 161 1434 2579 Fragments arranged by size: 2579 1434 1081 776 195 161 135 Bpu10I CC'TnA_GC Cuts at: 2736 2736 Size: 6361 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4486 5351 5649 4486 Size: 865 298 5198 Fragments arranged by size: 5198 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5024 882 Size: 617 3525 2219 Fragments arranged by size: 3525 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3064 4152 493 Size: 711 1860 1088 2702 Fragments arranged by size: 2702 1860 1088 711 BsaBI GATnn'nnATC Cuts at: 6355 6355 Size: 6361 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4168 4550 5185 275 Size: 53 83 186 936 2635 382 635 1451 Fragments arranged by size: 2635 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3535 4794 5192 5466 5613 6024 3535 Size: 1259 398 274 147 411 3872 Fragments arranged by size: 3872 1259 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3264 3294 1178 Size: 30 165 30 1861 30 4245 Fragments arranged by size: 4245 1861 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3093 1186 Size: 1907 4454 BseYI C'CCAG_C Cuts at: 2596 3257 3528 5564 6060 2596 Size: 661 271 2036 496 2897 Fragments arranged by size: 2897 2036 661 496 271 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2808 3185 3348 1163 Size: 573 1072 377 163 4176 Fragments arranged by size: 4176 1072 573 377 163 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3811 4572 5600 1540 Size: 275 186 1810 761 1028 2301 Fragments arranged by size: 2301 1810 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2203 3268 4422 4507 5578 2203 Size: 1065 1154 85 1071 2986 Fragments arranged by size: 2986 1154 1071 1065 85 BsmI GAATG_Cn' Cuts at: 2930 3872 3965 2930 Size: 942 93 5326 Fragments arranged by size: 5326 942 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2300 2300 Size: 6361 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2203 3268 3601 3680 4422 4507 5578 Size: 399 413 1065 333 79 742 85 1071 Cuts at: 5578 1391 Size: 2174 Fragments arranged by size: 2174 1071 1065 742 413 399 333 85 79 BspEI T'CCGG_A Cuts at: 6024 6024 Size: 6361 BspHI T'CATG_A Cuts at: 4250 5980 4250 Size: 1730 4631 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3716 844 Size: 2872 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4248 1394 Size: 429 2425 3507 Fragments arranged by size: 3507 2425 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2979 4856 5030 65 Size: 1183 1731 1877 174 1396 Fragments arranged by size: 1877 1731 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5005 5192 1700 Size: 110 3195 187 2869 Fragments arranged by size: 3195 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3229 96 Size: 3133 3228 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6361 BssSI C'ACGA_G Cuts at: 4421 5433 4421 Size: 1012 5349 BstAPI GCAn_nnn'nTGC Cuts at: 794 3371 3380 794 Size: 2577 9 3775 Fragments arranged by size: 3775 2577 9 BstBI TT'CG_AA Cuts at: 5165 5165 Size: 6361 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6361 BstXI CCAn_nnnn'nTGG Cuts at: 2689 2689 Size: 6361 BstYI r'GATC_y Cuts at: 1972 3288 3691 4058 4443 4460 5912 5998 6010 Size: 1316 403 367 385 17 1452 86 12 Cuts at: 6010 6356 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1316 403 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2687 2687 Size: 6361 BtgI C'CryG_G Cuts at: 513 1065 2004 3396 513 Size: 552 939 1392 3478 Fragments arranged by size: 3478 1392 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5622 Fragments arranged by size: 5622 683 56 BtsI GCAGTG_nn' Cuts at: 2444 2634 3877 4671 4691 2444 Size: 190 1243 794 20 4114 Fragments arranged by size: 4114 1243 794 190 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6361 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6326 DraI TTT'AAA Cuts at: 3665 3665 Size: 6361 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3221 5368 809 Size: 507 1905 2147 1802 Fragments arranged by size: 2147 1905 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2860 3808 4697 Size: 54 1425 50 275 18 1030 948 889 Cuts at: 4697 6326 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1030 948 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3808 1537 Size: 275 1996 4090 Fragments arranged by size: 4090 1996 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4291 1353 Size: 2938 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4948 5322 5468 387 Size: 1064 138 3359 374 146 1280 Fragments arranged by size: 3359 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2201 2201 Size: 6361 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2165 2234 2320 2547 2852 3096 3291 Size: 393 533 69 86 227 305 244 195 Cuts at: 3291 3426 3587 4424 5021 5808 6076 1239 Size: 135 161 837 597 787 268 1524 Fragments arranged by size: 1524 837 787 597 533 393 305 268 244 227 195 161 135 86 69 EcoNI CCTnn'n_nnAGG Cuts at: 2739 3075 3417 2739 Size: 336 342 5683 Fragments arranged by size: 5683 342 336 EcoO109I rG'GnC_Cy Cuts at: 2732 3598 2732 Size: 866 5495 EcoRI G'AATT_C Cuts at: 3670 4048 3670 Size: 378 5983 FspI TGC'GCA Cuts at: 4867 4867 Size: 6361 HaeII r_GCGC'y Cuts at: 1678 5508 1678 Size: 3830 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2000 2032 3540 3572 5002 Size: 32 135 32 428 32 1508 32 1430 Cuts at: 5002 5034 5076 5108 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1508 1430 428 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3101 3705 3951 669 Size: 988 281 1163 604 246 3079 Fragments arranged by size: 3079 1163 988 604 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6361 HpaI GTT'AAC Cuts at: 3951 3951 Size: 6361 KpnI G_GTAC'C Cuts at: 3686 3686 Size: 6361 MfeI C'AATT_G Cuts at: 2121 3960 2121 Size: 1839 4522 MluI A'CGCG_T Cuts at: 6237 6237 Size: 6361 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2243 3150 5475 5659 1305 Size: 938 907 2325 184 2007 Fragments arranged by size: 2325 2007 938 907 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4539 Fragments arranged by size: 4539 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2322 2657 4319 4678 4837 518 Size: 648 1156 335 1662 359 159 2042 Fragments arranged by size: 2042 1662 1156 648 359 335 159 MspA1I CmG'CkG Cuts at: 1606 3380 3461 3777 4454 5602 5847 1606 Size: 1774 81 316 677 1148 245 2120 Fragments arranged by size: 2120 1774 1148 677 316 245 81 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4870 Fragments arranged by size: 4870 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6361 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6361 NotI GC'GGCC_GC Cuts at: 3808 3808 Size: 6361 NsiI A_TGCA'T Cuts at: 3503 3503 Size: 6361 NspI r_CATG'y Cuts at: 1132 1229 2339 3719 5264 1132 Size: 97 1110 1380 1545 2229 Fragments arranged by size: 2229 1545 1380 1110 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6361 PciI A'CATG_T Cuts at: 2335 5260 2335 Size: 2925 3436 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6361 PfoI T'CCnGG_A Cuts at: 2896 2896 Size: 6361 PmeI GTTT'AAAC Cuts at: 3665 3665 Size: 6361 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3264 3296 4430 4462 5943 5975 3264 Size: 32 1134 32 1481 32 3650 Fragments arranged by size: 3650 1481 1134 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3489 1613 Size: 1876 4485 PsiI TTA'TAA Cuts at: 3931 3931 Size: 6361 PspOMI G'GGCC_C Cuts at: 3597 3597 Size: 6361 PstI C_TGCA'G Cuts at: 830 2451 3376 3713 830 Size: 1621 925 337 3478 Fragments arranged by size: 3478 1621 925 337 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6361 PvuII CAG'CTG Cuts at: 3380 3461 3380 Size: 81 6280 SacI G_AGCT'C Cuts at: 2203 2203 Size: 6361 SalI G'TCGA_C Cuts at: 1936 3703 1936 Size: 1767 4594 SbfI CC_TGCA'GG Cuts at: 3713 3713 Size: 6361 ScaI AGT'ACT Cuts at: 3621 4609 6321 3621 Size: 988 1712 3661 Fragments arranged by size: 3661 1712 988 SfcI C'TryA_G Cuts at: 826 1045 2447 3372 3709 4844 5525 5716 826 Size: 219 1402 925 337 1135 681 191 1471 Fragments arranged by size: 1471 1402 1135 925 681 337 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6361 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6361 SmaI CCC'GGG Cuts at: 1872 3688 1872 Size: 1816 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4465 5366 5628 820 Size: 197 926 2522 901 262 1553 Fragments arranged by size: 2522 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4152 493 Size: 3659 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6361 SphI G_CATG'C Cuts at: 1132 3719 1132 Size: 2587 3774 SspI AAT'ATT Cuts at: 5 52 2345 4285 5 Size: 47 2293 1940 2081 Fragments arranged by size: 2293 2081 1940 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6361 StyI C'CwwG_G Cuts at: 513 1065 2004 3800 513 Size: 552 939 1796 3074 Fragments arranged by size: 3074 1796 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4388 4405 4558 4743 5188 325 Size: 1255 2808 17 153 185 445 1498 Fragments arranged by size: 2808 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3229 3619 4607 Size: 274 80 33 51 1443 1252 390 988 Cuts at: 4607 6319 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1252 988 390 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6321 6353 6321 Size: 32 6329 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6361 XbaI T'CTAG_A Cuts at: 3697 3697 Size: 6361 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2087 2656 2087 Size: 569 5792 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6361 XmaI C'CCGG_G Cuts at: 1870 3686 1870 Size: 1816 4545 XmnI GAAnn'nnTTC Cuts at: 3621 4490 3621 Size: 869 5492 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4169 276 Size: 53 83 186 936 2635 2468 Fragments arranged by size: 2635 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BsiWI Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI