(Circular) (Six-base) MAPSORT of: pFN21AB5679.seq Check: 5798 from: 1 to: 7585 pFN21AB5679 7585 bp With 182 enzymes: SgfI * October 20, 2009 10:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3047 4600 5395 278 Size: 53 83 186 936 1511 1553 795 2468 Fragments arranged by size: 2468 1553 1511 936 795 186 83 53 AccI GT'mk_AC Cuts at: 1937 4928 1937 Size: 2991 4594 Acc65I G'GTAC_C Cuts at: 3753 4906 3753 Size: 1153 6432 AclI AA'CG_TT Cuts at: 1563 5712 6085 1563 Size: 4149 373 3063 Fragments arranged by size: 4149 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2354 2936 4169 4538 5648 7032 7300 1632 Size: 722 582 1233 369 1110 1384 268 1917 Fragments arranged by size: 1917 1384 1233 1110 722 582 369 268 AfeI AGC'GCT Cuts at: 3698 3698 Size: 7585 AflII C'TTAA_G Cuts at: 820 1017 3176 820 Size: 197 2159 5229 Fragments arranged by size: 5229 2159 197 AflIII A'CryG_T Cuts at: 1203 6484 7461 1203 Size: 5281 977 1327 Fragments arranged by size: 5281 1327 977 AgeI A'CCGG_T Cuts at: 6416 6416 Size: 7585 AhdI GACnn_n'nnGTC Cuts at: 6314 6314 Size: 7585 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7585 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7585 AlwNI CAG_nnn'CTG Cuts at: 5328 6900 5328 Size: 1572 6013 ApaI G_GGCC'C Cuts at: 3742 3742 Size: 7585 ApaLI G'TGCA_C Cuts at: 5642 6798 5642 Size: 1156 6429 ApoI r'AATT_y Cuts at: 1493 2580 3295 3422 3869 4473 4661 4894 5120 Size: 1087 715 127 447 604 188 233 226 Cuts at: 5120 5272 7381 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1087 715 604 447 233 226 188 152 127 AseI AT'TA_AT Cuts at: 160 1032 6139 160 Size: 872 5107 1606 Fragments arranged by size: 5107 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7585 AvaI C'yCGr_G Cuts at: 1870 1943 4910 1870 Size: 73 2967 4545 Fragments arranged by size: 4545 2967 73 BamHI G'GATC_C Cuts at: 2149 3553 4915 5282 2149 Size: 1404 1362 367 4452 Fragments arranged by size: 4452 1404 1362 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3753 4906 6361 618 Size: 325 217 627 1966 1153 1455 1842 Fragments arranged by size: 1966 1842 1455 1153 627 325 217 BanII G_rGCy'C Cuts at: 1391 3742 4235 4676 4904 1391 Size: 2351 493 441 228 4072 Fragments arranged by size: 4072 2351 493 441 228 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3729 928 Size: 971 1830 4784 Fragments arranged by size: 4784 1830 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5776 5810 1903 Size: 34 3839 34 3678 Fragments arranged by size: 3839 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5477 6687 5477 Size: 1210 6375 BclI T'GATC_A Cuts at: 1548 4692 1548 Size: 3144 4441 BfrBI ATG'CAT Cuts at: 2103 2867 2103 Size: 764 6821 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6196 136 Size: 107 122 71 631 605 4524 1525 Fragments arranged by size: 4524 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2344 7580 2344 Size: 5236 2349 BlpI GC'TnA_GC Cuts at: 5002 5002 Size: 7585 Bme1580I G_kGCm'C Cuts at: 1790 3742 5646 6802 1790 Size: 1952 1904 1156 2573 Fragments arranged by size: 2573 1952 1904 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7585 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2159 2208 2314 Size: 416 53 83 399 24 742 49 106 Cuts at: 2314 4324 5316 6274 442 Size: 2010 992 958 1753 Fragments arranged by size: 2010 1753 992 958 742 416 399 106 83 53 49 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7585 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3024 3617 3689 4240 4859 6245 1239 Size: 1785 593 72 551 619 1386 2579 Fragments arranged by size: 2579 1785 1386 619 593 551 72 Bpu10I CC'TnA_GC Cuts at: 2204 2204 Size: 7585 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3383 4154 4758 4787 5710 6575 6873 3383 Size: 771 604 29 923 865 298 4095 Fragments arranged by size: 4095 923 865 771 604 298 29 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6248 882 Size: 617 4749 2219 Fragments arranged by size: 4749 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5376 493 Size: 711 4172 2702 Fragments arranged by size: 4172 2702 711 BsaBI GATnn'nnATC Cuts at: 4694 7579 4694 Size: 2885 4700 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3044 4597 5392 5774 Size: 53 83 186 936 1511 1553 795 382 Cuts at: 5774 6409 275 Size: 635 1451 Fragments arranged by size: 1553 1511 1451 936 795 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6018 6416 6690 6837 7248 6018 Size: 398 274 147 411 6355 Fragments arranged by size: 6355 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7360 Fragments arranged by size: 7360 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3431 1186 Size: 2245 5340 BseYI C'CCAG_C Cuts at: 6788 7284 6788 Size: 496 7089 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4038 1163 Size: 573 2302 4710 Fragments arranged by size: 4710 2302 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5035 5796 6824 1540 Size: 275 186 3034 761 1028 2301 Fragments arranged by size: 3034 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4402 5646 5731 6802 4402 Size: 1244 85 1071 5185 Fragments arranged by size: 5185 1244 1071 85 BsmI GAATG_Cn' Cuts at: 2365 5096 5189 2365 Size: 2731 93 4761 Fragments arranged by size: 4761 2731 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3035 3035 Size: 7585 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3742 4235 4402 4676 4904 5646 5731 Size: 399 1952 493 167 274 228 742 85 Cuts at: 5731 6802 1391 Size: 1071 2174 Fragments arranged by size: 2174 1952 1071 742 493 399 274 228 167 85 BspEI T'CCGG_A Cuts at: 7248 7248 Size: 7585 BspHI T'CATG_A Cuts at: 2437 3994 5474 7204 2437 Size: 1557 1480 1730 2818 Fragments arranged by size: 2818 1730 1557 1480 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4940 844 Size: 4096 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4257 5472 1394 Size: 429 2434 1215 3507 Fragments arranged by size: 3507 2434 1215 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2028 2317 6080 6254 65 Size: 1183 780 289 3763 174 1396 Fragments arranged by size: 3763 1396 1183 780 289 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6229 6416 1700 Size: 110 4419 187 2869 Fragments arranged by size: 4419 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7585 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7585 BssSI C'ACGA_G Cuts at: 3784 5645 6657 3784 Size: 1861 1012 4712 Fragments arranged by size: 4712 1861 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7585 BstBI TT'CG_AA Cuts at: 6389 6389 Size: 7585 BstEII G'GTnAC_C Cuts at: 1184 3094 1184 Size: 1910 5675 BstXI CCAn_nnnn'nTGG Cuts at: 3134 3134 Size: 7585 BstYI r'GATC_y Cuts at: 1972 2149 2344 3553 3841 4915 5282 5667 5684 Size: 177 195 1209 288 1074 367 385 17 Cuts at: 5684 7136 7222 7234 7580 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1209 1074 385 367 346 288 195 177 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3972 3972 Size: 7585 BtgI C'CryG_G Cuts at: 513 1065 3009 513 Size: 552 1944 5089 Fragments arranged by size: 5089 1944 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6846 Fragments arranged by size: 6846 683 56 BtsI GCAGTG_nn' Cuts at: 2450 3047 5101 5895 5915 2450 Size: 597 2054 794 20 4120 Fragments arranged by size: 4120 2054 794 597 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7585 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7550 DraI TTT'AAA Cuts at: 4377 4889 4377 Size: 512 7073 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2246 6592 809 Size: 507 930 4346 1802 Fragments arranged by size: 4346 1802 930 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 5032 5921 7550 Size: 54 1425 50 275 18 3202 889 1629 Cuts at: 7550 8 Size: 43 Fragments arranged by size: 3202 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5032 1537 Size: 275 3220 4090 Fragments arranged by size: 4090 3220 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2674 3076 4328 5515 1353 Size: 1321 402 1252 1187 3423 Fragments arranged by size: 3423 1321 1252 1187 402 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6172 6546 6692 387 Size: 1064 138 4583 374 146 1280 Fragments arranged by size: 4583 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2354 2936 3024 3617 3689 4169 4240 Size: 393 722 582 88 593 72 480 71 Cuts at: 4240 4538 4859 5648 6245 7032 7300 1239 Size: 298 321 789 597 787 268 1524 Fragments arranged by size: 1524 789 787 722 597 593 582 480 393 321 298 268 88 72 71 EcoO109I rG'GnC_Cy Cuts at: 3022 3738 3739 3022 Size: 716 1 6868 Fragments arranged by size: 6868 716 1 EcoRI G'AATT_C Cuts at: 2580 3295 4894 5272 2580 Size: 715 1599 378 4893 Fragments arranged by size: 4893 1599 715 378 EcoRV GAT'ATC Cuts at: 2950 4156 2950 Size: 1206 6379 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2762 2762 Size: 7585 FspI TGC'GCA Cuts at: 6091 6091 Size: 7585 HaeII r_GCGC'y Cuts at: 1678 3700 6732 1678 Size: 2022 3032 2531 Fragments arranged by size: 3032 2531 2022 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2804 2836 3515 3547 3820 Size: 32 135 32 1232 32 679 32 273 Cuts at: 3820 3852 4217 4249 4486 4518 4658 4690 6226 Size: 32 365 32 237 32 140 32 1536 Cuts at: 6226 6258 6300 6332 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1536 1232 679 365 273 237 140 135 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4491 4929 5175 669 Size: 988 281 2553 438 246 3079 Fragments arranged by size: 3079 2553 988 438 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7585 HpaI GTT'AAC Cuts at: 5175 5175 Size: 7585 KpnI G_GTAC'C Cuts at: 3757 4910 3757 Size: 1153 6432 MfeI C'AATT_G Cuts at: 2727 5184 2727 Size: 2457 5128 MluI A'CGCG_T Cuts at: 7461 7461 Size: 7585 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2730 3706 4090 4877 6699 6883 1305 Size: 1425 976 384 787 1822 184 2007 Fragments arranged by size: 2007 1822 1425 976 787 384 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5763 Fragments arranged by size: 5763 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4688 5543 5902 6061 518 Size: 648 3522 855 359 159 2042 Fragments arranged by size: 3522 2042 855 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3323 3943 5001 5678 6826 7071 1606 Size: 1717 620 1058 677 1148 245 2120 Fragments arranged by size: 2120 1717 1148 1058 677 620 245 NcoI C'CATG_G Cuts at: 513 1065 3009 513 Size: 552 1944 5089 Fragments arranged by size: 5089 1944 552 NdeI CA'TA_TG Cuts at: 387 4678 387 Size: 4291 3294 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7585 NotI GC'GGCC_GC Cuts at: 5032 5032 Size: 7585 NsiI A_TGCA'T Cuts at: 2105 2869 2105 Size: 764 6821 NspI r_CATG'y Cuts at: 1132 1229 2713 3577 4943 6488 1132 Size: 97 1484 864 1366 1545 2229 Fragments arranged by size: 2229 1545 1484 1366 864 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7585 PciI A'CATG_T Cuts at: 6484 6484 Size: 7585 PflMI CCAn_nnn'nTGG Cuts at: 1108 3118 4132 4336 1108 Size: 2010 1014 204 4357 Fragments arranged by size: 4357 2010 1014 204 PfoI T'CCnGG_A Cuts at: 3717 3717 Size: 7585 PmeI GTTT'AAAC Cuts at: 4889 4889 Size: 7585 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5654 5686 7167 7199 5654 Size: 32 1481 32 6040 Fragments arranged by size: 6040 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7585 PsiI TTA'TAA Cuts at: 2544 5155 2544 Size: 2611 4974 PspOMI G'GGCC_C Cuts at: 3738 3738 Size: 7585 PstI C_TGCA'G Cuts at: 830 3747 4937 830 Size: 2917 1190 3478 Fragments arranged by size: 3478 2917 1190 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7585 PvuII CAG'CTG Cuts at: 3943 3943 Size: 7585 SalI G'TCGA_C Cuts at: 1936 4927 1936 Size: 2991 4594 SbfI CC_TGCA'GG Cuts at: 3747 4937 3747 Size: 1190 6395 ScaI AGT'ACT Cuts at: 2942 3446 5833 7545 2942 Size: 504 2387 1712 2982 Fragments arranged by size: 2982 2387 1712 504 SexAI A'CCwGG_T Cuts at: 3141 3141 Size: 7585 SfcI C'TryA_G Cuts at: 826 1045 3463 3743 4933 6068 6749 6940 826 Size: 219 2418 280 1190 1135 681 191 1471 Fragments arranged by size: 2418 1471 1190 1135 681 280 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7585 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7585 SmaI CCC'GGG Cuts at: 1872 4912 1872 Size: 3040 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3176 3398 4169 4766 4773 5689 Size: 197 926 1233 222 771 597 7 916 Cuts at: 5689 6590 6852 820 Size: 901 262 1553 Fragments arranged by size: 1553 1233 926 916 901 771 597 262 222 197 7 SnaBI TAC'GTA Cuts at: 493 5376 493 Size: 4883 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7585 SphI G_CATG'C Cuts at: 1132 4943 1132 Size: 3811 3774 SspI AAT'ATT Cuts at: 5 52 4147 5509 5 Size: 47 4095 1362 2081 Fragments arranged by size: 4095 2081 1362 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7585 StyI C'CwwG_G Cuts at: 513 1065 2192 3009 4725 5024 513 Size: 552 1127 817 1716 299 3074 Fragments arranged by size: 3074 1716 1127 817 552 299 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3401 3853 4385 5612 5629 5782 5967 Size: 1255 1821 452 532 1227 17 153 185 Cuts at: 5967 6412 325 Size: 445 1498 Fragments arranged by size: 1821 1498 1255 1227 532 452 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2783 2940 3444 Size: 274 80 33 51 1443 806 157 504 Cuts at: 3444 5831 7543 96 Size: 2387 1712 138 Fragments arranged by size: 2387 1712 1443 806 504 274 157 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2134 2166 4706 4738 7545 7577 2134 Size: 32 2540 32 2807 32 2142 Fragments arranged by size: 2807 2540 2142 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7585 XbaI T'CTAG_A Cuts at: 4921 4921 Size: 7585 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2825 2825 Size: 7585 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7585 XmaI C'CCGG_G Cuts at: 1870 4910 1870 Size: 3040 4545 XmnI GAAnn'nnTTC Cuts at: 2187 5714 2187 Size: 3527 4058 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3045 4598 5393 276 Size: 53 83 186 936 1511 1553 795 2468 Fragments arranged by size: 2468 1553 1511 936 795 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BstZ17I DraIII EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI