(Circular) (Six-base) MAPSORT of: pFN21AB5642.seq Check: 6717 from: 1 to: 5755 pFN21AB5642 5755 bp With 182 enzymes: SgfI * October 20, 2009 10:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3002 3002 Size: 5755 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2271 3565 278 Size: 53 83 186 936 735 1294 2468 Fragments arranged by size: 2468 1294 936 735 186 83 53 AccI GT'mk_AC Cuts at: 1937 3098 1937 Size: 1161 4594 Acc65I G'GTAC_C Cuts at: 2556 3076 2556 Size: 520 5235 AclI AA'CG_TT Cuts at: 1563 3882 4255 1563 Size: 2319 373 3063 Fragments arranged by size: 3063 2319 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3818 5202 5470 1632 Size: 2186 1384 268 1917 Fragments arranged by size: 2186 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5558 AflIII A'CryG_T Cuts at: 1203 4654 5631 1203 Size: 3451 977 1327 Fragments arranged by size: 3451 1327 977 AgeI A'CCGG_T Cuts at: 4586 4586 Size: 5755 AhdI GACnn_n'nnGTC Cuts at: 4484 4484 Size: 5755 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5755 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5755 AlwNI CAG_nnn'CTG Cuts at: 2539 3498 5070 2539 Size: 959 1572 3224 Fragments arranged by size: 3224 1572 959 ApaLI G'TGCA_C Cuts at: 3812 4968 3812 Size: 1156 4599 ApoI r'AATT_y Cuts at: 1493 3064 3290 3442 5551 1493 Size: 1571 226 152 2109 1697 Fragments arranged by size: 2109 1697 1571 226 152 AseI AT'TA_AT Cuts at: 160 1032 4309 160 Size: 872 3277 1606 Fragments arranged by size: 3277 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5755 AvaI C'yCGr_G Cuts at: 1870 1943 2095 2405 2745 3080 1870 Size: 73 152 310 340 335 4545 Fragments arranged by size: 4545 340 335 310 152 73 BamHI G'GATC_C Cuts at: 3085 3452 3085 Size: 367 5388 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2084 2252 2320 2556 2579 Size: 325 217 627 297 168 68 236 23 Cuts at: 2579 2816 3076 4531 618 Size: 237 260 1455 1842 Fragments arranged by size: 1842 1455 627 325 297 260 237 236 217 168 68 23 BanII G_rGCy'C Cuts at: 1391 3074 1391 Size: 1683 4072 BbeI G_GCGC'C Cuts at: 2088 2256 2324 2583 2088 Size: 168 68 259 5260 Fragments arranged by size: 5260 259 168 68 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2730 2846 928 Size: 971 831 116 3837 Fragments arranged by size: 3837 971 831 116 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3946 3980 1903 Size: 34 2009 34 3678 Fragments arranged by size: 3678 2009 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3647 4857 3647 Size: 1210 4545 BclI T'GATC_A Cuts at: 1548 1548 Size: 5755 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2825 4366 136 Size: 107 122 71 631 605 1153 1541 1525 Fragments arranged by size: 1541 1525 1153 631 605 122 107 71 BglII A'GATC_T Cuts at: 5750 5750 Size: 5755 BlpI GC'TnA_GC Cuts at: 3172 3172 Size: 5755 Bme1580I G_kGCm'C Cuts at: 1790 3816 4972 1790 Size: 2026 1156 2573 Fragments arranged by size: 2573 2026 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5755 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2794 3486 4444 Size: 416 53 83 399 24 1377 692 958 Cuts at: 4444 442 Size: 1753 Fragments arranged by size: 1753 1377 958 692 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5755 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2566 2566 Size: 5755 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2456 2561 2654 3042 4415 1239 Size: 1217 105 93 388 1373 2579 Fragments arranged by size: 2579 1373 1217 388 105 93 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2348 3880 4745 5043 2348 Size: 1532 865 298 3060 Fragments arranged by size: 3060 1532 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2630 4418 882 Size: 617 1131 1788 2219 Fragments arranged by size: 2219 1788 1131 617 BsaAI yAC'GTr Cuts at: 493 1204 3546 493 Size: 711 2342 2702 Fragments arranged by size: 2702 2342 711 BsaBI GATnn'nnATC Cuts at: 5749 5749 Size: 5755 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2085 2253 2268 2321 Size: 53 83 186 936 552 168 15 53 Cuts at: 2321 2355 2492 2580 3562 3944 4579 275 Size: 34 137 88 982 382 635 1451 Fragments arranged by size: 1451 982 936 635 552 382 186 168 137 88 83 53 53 34 15 BsaWI w'CCGG_w Cuts at: 4188 4586 4860 5007 5418 4188 Size: 398 274 147 411 4525 Fragments arranged by size: 4525 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2507 2537 1178 Size: 30 165 30 1104 30 4396 Fragments arranged by size: 4396 1104 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2345 2390 2522 2525 2528 1186 Size: 1159 45 132 3 3 4413 Fragments arranged by size: 4413 1159 132 45 3 3 BseYI C'CCAG_C Cuts at: 2937 4958 5454 2937 Size: 2021 496 3238 Fragments arranged by size: 3238 2021 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2684 1163 Size: 573 948 4234 Fragments arranged by size: 4234 948 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2240 2349 2417 3205 3966 4994 Size: 275 186 239 109 68 788 761 1028 Cuts at: 4994 1540 Size: 2301 Fragments arranged by size: 2301 1028 788 761 275 239 186 109 68 BsiHKAI G_wGCw'C Cuts at: 3816 3901 4972 3816 Size: 85 1071 4599 Fragments arranged by size: 4599 1071 85 BsmI GAATG_Cn' Cuts at: 3266 3359 3266 Size: 93 5662 BsmBI CGTCTCn'nnnn_ Cuts at: 2672 2672 Size: 5755 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3074 3816 3901 4972 1391 Size: 399 1284 742 85 1071 2174 Fragments arranged by size: 2174 1284 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5418 5418 Size: 5755 BspHI T'CATG_A Cuts at: 3644 5374 3644 Size: 1730 4025 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2595 3002 3110 844 Size: 1751 407 108 3489 Fragments arranged by size: 3489 1751 407 108 BsrBI CCG'CTC Cuts at: 1394 1823 3642 1394 Size: 429 1819 3507 Fragments arranged by size: 3507 1819 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4250 4424 65 Size: 1183 3002 174 1396 Fragments arranged by size: 3002 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2060 2072 2162 2186 2416 2529 2576 Size: 110 250 12 90 24 230 113 47 Cuts at: 2576 4399 4586 1700 Size: 1823 187 2869 Fragments arranged by size: 2869 1823 250 230 187 113 110 90 47 24 12 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5755 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5755 BssSI C'ACGA_G Cuts at: 2675 3815 4827 2675 Size: 1140 1012 3603 Fragments arranged by size: 3603 1140 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3017 794 Size: 2223 3532 BstBI TT'CG_AA Cuts at: 4559 4559 Size: 5755 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5755 BstYI r'GATC_y Cuts at: 1972 3085 3452 3837 3854 5306 5392 5404 5750 Size: 1113 367 385 17 1452 86 12 346 Cuts at: 5750 1972 Size: 1977 Fragments arranged by size: 1977 1452 1113 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5203 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5016 Fragments arranged by size: 5016 683 56 BtsI GCAGTG_nn' Cuts at: 3271 4065 4085 3271 Size: 794 20 4941 Fragments arranged by size: 4941 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5755 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5720 DraI TTT'AAA Cuts at: 3059 3059 Size: 5755 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4762 809 Size: 507 3446 1802 Fragments arranged by size: 3446 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2237 2346 2414 Size: 54 1425 50 275 18 407 109 68 Cuts at: 2414 3202 4091 5720 8 Size: 788 889 1629 43 Fragments arranged by size: 1629 1425 889 788 407 275 109 68 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2237 2346 2414 3202 1537 Size: 275 425 109 68 788 4090 Fragments arranged by size: 4090 788 425 275 109 68 EarI CTCTTCn'nnn_ Cuts at: 1353 2367 3685 1353 Size: 1014 1318 3423 Fragments arranged by size: 3423 1318 1014 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2193 4342 4716 4862 387 Size: 1064 138 604 2149 374 146 1280 Fragments arranged by size: 2149 1280 1064 604 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2456 2561 2654 3042 3818 4415 5202 Size: 393 824 105 93 388 776 597 787 Cuts at: 5202 5470 1239 Size: 268 1524 Fragments arranged by size: 1524 824 787 776 597 393 388 268 105 93 EcoO109I rG'GnC_Cy Cuts at: 2260 2971 2260 Size: 711 5044 EcoRI G'AATT_C Cuts at: 3064 3442 3064 Size: 378 5377 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2714 2714 Size: 5755 FspI TGC'GCA Cuts at: 4261 4261 Size: 5755 HaeII r_GCGC'y Cuts at: 1678 2088 2187 2256 2324 2583 2633 4902 1678 Size: 410 99 69 68 259 50 2269 2531 Fragments arranged by size: 2531 2269 410 259 99 69 68 50 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2028 2060 2536 2568 2720 Size: 32 135 32 456 32 476 32 152 Cuts at: 2720 2739 2752 2771 2785 2817 4396 4428 4470 Size: 19 13 19 14 32 1579 32 42 Cuts at: 4470 4502 1373 Size: 32 2626 Fragments arranged by size: 2626 1579 476 456 152 135 42 32 32 32 32 32 32 32 19 19 14 13 HincII GTy'rAC Cuts at: 669 1657 1938 3099 3345 669 Size: 988 281 1161 246 3079 Fragments arranged by size: 3079 1161 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5755 HpaI GTT'AAC Cuts at: 3345 3345 Size: 5755 KasI G'GCGC_C Cuts at: 2084 2252 2320 2579 2084 Size: 168 68 259 5260 Fragments arranged by size: 5260 259 168 68 KpnI G_GTAC'C Cuts at: 2560 3080 2560 Size: 520 5235 MfeI C'AATT_G Cuts at: 3354 3354 Size: 5755 MluI A'CGCG_T Cuts at: 5631 5631 Size: 5755 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4869 5053 1305 Size: 3564 184 2007 Fragments arranged by size: 3564 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3933 Fragments arranged by size: 3933 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3713 4072 4231 518 Size: 648 2547 359 159 2042 Fragments arranged by size: 2547 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2079 2351 2431 3171 3848 4996 5241 1606 Size: 473 272 80 740 677 1148 245 2120 Fragments arranged by size: 2120 1148 740 677 473 272 245 80 NaeI GCC'GGC Cuts at: 2062 2074 2164 2188 2418 2062 Size: 12 90 24 230 5399 Fragments arranged by size: 5399 230 90 24 12 NarI GG'CG_CC Cuts at: 2085 2253 2321 2580 2085 Size: 168 68 259 5260 Fragments arranged by size: 5260 259 168 68 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5203 NdeI CA'TA_TG Cuts at: 387 387 Size: 5755 NgoMIV G'CCGG_C Cuts at: 2060 2072 2162 2186 2416 2060 Size: 12 90 24 230 5399 Fragments arranged by size: 5399 230 90 24 12 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5755 NotI GC'GGCC_GC Cuts at: 2237 3202 2237 Size: 965 4790 NspI r_CATG'y Cuts at: 1132 1229 2698 3113 4658 1132 Size: 97 1469 415 1545 2229 Fragments arranged by size: 2229 1545 1469 415 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5755 PciI A'CATG_T Cuts at: 4654 4654 Size: 5755 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5755 PfoI T'CCnGG_A Cuts at: 2222 2222 Size: 5755 PmeI GTTT'AAAC Cuts at: 3059 3059 Size: 5755 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3824 3856 5337 5369 3824 Size: 32 1481 32 4210 Fragments arranged by size: 4210 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2260 2971 2260 Size: 711 5044 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5755 PsiI TTA'TAA Cuts at: 3325 3325 Size: 5755 PstI C_TGCA'G Cuts at: 830 3107 830 Size: 2277 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5755 SalI G'TCGA_C Cuts at: 1936 3097 1936 Size: 1161 4594 SbfI CC_TGCA'GG Cuts at: 3107 3107 Size: 5755 ScaI AGT'ACT Cuts at: 4003 5715 4003 Size: 1712 4043 SfcI C'TryA_G Cuts at: 826 1045 3103 4238 4919 5110 826 Size: 219 2058 1135 681 191 1471 Fragments arranged by size: 2058 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2086 2254 2322 2581 2086 Size: 168 68 259 5260 Fragments arranged by size: 5260 259 168 68 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5755 SgrAI Cr'CCGG_yG Cuts at: 1700 2186 2576 1700 Size: 486 390 4879 Fragments arranged by size: 4879 486 390 SmaI CCC'GGG Cuts at: 1872 2097 3082 1872 Size: 225 985 4545 Fragments arranged by size: 4545 985 225 SmlI C'TyrA_G Cuts at: 820 1017 1943 2327 2745 3859 4760 5022 820 Size: 197 926 384 418 1114 901 262 1553 Fragments arranged by size: 1553 1114 926 901 418 384 262 197 SnaBI TAC'GTA Cuts at: 493 3546 493 Size: 3053 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5755 SphI G_CATG'C Cuts at: 1132 2698 3113 1132 Size: 1566 415 3774 Fragments arranged by size: 3774 1566 415 SspI AAT'ATT Cuts at: 5 52 3679 5 Size: 47 3627 2081 Fragments arranged by size: 3627 2081 47 StuI AGG'CCT Cuts at: 1517 2129 1517 Size: 612 5143 StyI C'CwwG_G Cuts at: 513 1065 2583 2689 3194 513 Size: 552 1518 106 505 3074 Fragments arranged by size: 3074 1518 552 505 106 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3782 3799 3952 4137 4582 325 Size: 1255 2202 17 153 185 445 1498 Fragments arranged by size: 2202 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4001 5713 96 Size: 274 80 33 51 1443 2024 1712 138 Fragments arranged by size: 2024 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5715 5747 5715 Size: 32 5723 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5755 XbaI T'CTAG_A Cuts at: 3091 3091 Size: 5755 XhoI C'TCGA_G Cuts at: 1943 2745 1943 Size: 802 4953 XmaI C'CCGG_G Cuts at: 1870 2095 3080 1870 Size: 225 985 4545 Fragments arranged by size: 4545 985 225 XmnI GAAnn'nnTTC Cuts at: 3884 3884 Size: 5755 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2269 3563 276 Size: 53 83 186 936 735 1294 2468 Fragments arranged by size: 2468 1294 936 735 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI