(Circular) (Six-base) MAPSORT of: pFN21AB5639.seq Check: 2126 from: 1 to: 6025 pFN21AB5639 6025 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3835 278 Size: 53 83 186 936 2299 2468 Fragments arranged by size: 2468 2299 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3368 1937 Size: 1431 4594 Acc65I G'GTAC_C Cuts at: 3346 3346 Size: 6025 AclI AA'CG_TT Cuts at: 1563 4152 4525 1563 Size: 2589 373 3063 Fragments arranged by size: 3063 2589 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4088 5472 5740 1632 Size: 2456 1384 268 1917 Fragments arranged by size: 2456 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5828 AflIII A'CryG_T Cuts at: 1203 2907 4924 5901 1203 Size: 1704 2017 977 1327 Fragments arranged by size: 2017 1704 1327 977 AgeI A'CCGG_T Cuts at: 4856 4856 Size: 6025 AhdI GACnn_n'nnGTC Cuts at: 4754 4754 Size: 6025 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6025 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6025 AlwNI CAG_nnn'CTG Cuts at: 2954 3314 3768 5340 2954 Size: 360 454 1572 3639 Fragments arranged by size: 3639 1572 454 360 ApaI G_GGCC'C Cuts at: 2047 2047 Size: 6025 ApaLI G'TGCA_C Cuts at: 4082 5238 4082 Size: 1156 4869 ApoI r'AATT_y Cuts at: 1493 3334 3560 3712 5821 1493 Size: 1841 226 152 2109 1697 Fragments arranged by size: 2109 1841 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4579 160 Size: 872 3547 1606 Fragments arranged by size: 3547 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6025 AvaI C'yCGr_G Cuts at: 1870 1943 2918 3350 1870 Size: 73 975 432 4545 Fragments arranged by size: 4545 975 432 73 BamHI G'GATC_C Cuts at: 3355 3722 3355 Size: 367 5658 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2429 3049 3346 4801 618 Size: 325 217 627 642 620 297 1455 1842 Fragments arranged by size: 1842 1455 642 627 620 325 297 217 BanII G_rGCy'C Cuts at: 1391 2047 2100 3278 3344 1391 Size: 656 53 1178 66 4072 Fragments arranged by size: 4072 1178 656 66 53 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2277 928 Size: 971 378 4676 Fragments arranged by size: 4676 971 378 BbvCI CC'TCA_GC Cuts at: 2805 3123 2805 Size: 318 5707 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3020 3054 4216 4250 1903 Size: 34 1083 34 1162 34 3678 Fragments arranged by size: 3678 1162 1083 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3917 5127 3917 Size: 1210 4815 BclI T'GATC_A Cuts at: 1548 2169 1548 Size: 621 5404 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4636 136 Size: 107 122 71 631 605 2964 1525 Fragments arranged by size: 2964 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6020 6020 Size: 6025 BlpI GC'TnA_GC Cuts at: 3442 3442 Size: 6025 Bme1580I G_kGCm'C Cuts at: 1790 2047 3054 4086 5242 1790 Size: 257 1007 1032 1156 2573 Fragments arranged by size: 2573 1156 1032 1007 257 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6025 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3756 4714 442 Size: 416 53 83 399 24 2339 958 1753 Fragments arranged by size: 2339 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6025 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2542 2587 4685 1239 Size: 1303 45 2098 2579 Fragments arranged by size: 2579 2098 1303 45 Bpu10I CC'TnA_GC Cuts at: 2749 2769 2805 2859 3123 2749 Size: 20 36 54 264 5651 Fragments arranged by size: 5651 264 54 36 20 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4150 5015 5313 4150 Size: 865 298 4862 Fragments arranged by size: 4862 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2155 2967 4688 882 Size: 617 656 812 1721 2219 Fragments arranged by size: 2219 1721 812 656 617 BsaAI yAC'GTr Cuts at: 493 1204 3816 493 Size: 711 2612 2702 Fragments arranged by size: 2702 2612 711 BsaBI GATnn'nnATC Cuts at: 6019 6019 Size: 6025 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3832 4214 4849 275 Size: 53 83 186 936 2299 382 635 1451 Fragments arranged by size: 2299 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4458 4856 5130 5277 5688 4458 Size: 398 274 147 411 4795 Fragments arranged by size: 4795 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2646 2676 1178 Size: 30 165 30 1243 30 4527 Fragments arranged by size: 4527 1243 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2603 2983 2986 1186 Size: 1417 380 3 4225 Fragments arranged by size: 4225 1417 380 3 BseYI C'CCAG_C Cuts at: 2193 2578 3229 5228 5724 2193 Size: 385 651 1999 496 2494 Fragments arranged by size: 2494 1999 651 496 385 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2335 2374 1163 Size: 573 599 39 4814 Fragments arranged by size: 4814 599 573 39 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2034 3475 4236 5264 1540 Size: 275 186 33 1441 761 1028 2301 Fragments arranged by size: 2301 1441 1028 761 275 186 33 BsiHKAI G_wGCw'C Cuts at: 2124 3278 4086 4171 5242 2124 Size: 1154 808 85 1071 2907 Fragments arranged by size: 2907 1154 1071 808 85 BsmI GAATG_Cn' Cuts at: 3536 3629 3536 Size: 93 5932 BsmBI CGTCTCn'nnnn_ Cuts at: 2585 3218 2585 Size: 633 5392 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2047 2100 2124 3054 3278 3344 4086 Size: 399 257 53 24 930 224 66 742 Cuts at: 4086 4171 5242 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 930 742 399 257 224 85 66 53 24 BspEI T'CCGG_A Cuts at: 5688 5688 Size: 6025 BspHI T'CATG_A Cuts at: 3075 3914 5644 3075 Size: 839 1730 3456 Fragments arranged by size: 3456 1730 839 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3380 844 Size: 2536 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2204 3073 3912 1394 Size: 429 381 869 839 3507 Fragments arranged by size: 3507 869 839 429 381 BsrDI GCAATG_nn' Cuts at: 65 1248 4520 4694 65 Size: 1183 3272 174 1396 Fragments arranged by size: 3272 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2944 4669 4856 1700 Size: 110 1134 1725 187 2869 Fragments arranged by size: 2869 1725 1134 187 110 BsrGI T'GTAC_A Cuts at: 96 2513 3150 96 Size: 2417 637 2971 Fragments arranged by size: 2971 2417 637 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6025 BssSI C'ACGA_G Cuts at: 4085 5097 4085 Size: 1012 5013 BstAPI GCAn_nnn'nTGC Cuts at: 794 2292 3314 794 Size: 1498 1022 3505 Fragments arranged by size: 3505 1498 1022 BstBI TT'CG_AA Cuts at: 4829 4829 Size: 6025 BstEII G'GTnAC_C Cuts at: 1184 2596 1184 Size: 1412 4613 BstXI CCAn_nnnn'nTGG Cuts at: 2494 2494 Size: 6025 BstYI r'GATC_y Cuts at: 1972 3355 3722 4107 4124 5576 5662 5674 6020 Size: 1383 367 385 17 1452 86 12 346 Cuts at: 6020 1972 Size: 1977 Fragments arranged by size: 1977 1452 1383 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2133 513 Size: 552 939 129 4405 Fragments arranged by size: 4405 939 552 129 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5286 Fragments arranged by size: 5286 683 56 BtsI GCAGTG_nn' Cuts at: 3541 4335 4355 3541 Size: 794 20 5211 Fragments arranged by size: 5211 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6025 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5990 DraI TTT'AAA Cuts at: 3329 3329 Size: 6025 DraIII CAC_nnn'GTG Cuts at: 2358 2412 2481 2358 Size: 54 69 5902 Fragments arranged by size: 5902 69 54 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5032 809 Size: 507 3716 1802 Fragments arranged by size: 3716 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2031 3472 4361 Size: 54 1425 50 275 18 201 1441 889 Cuts at: 4361 5990 8 Size: 1629 43 Fragments arranged by size: 1629 1441 1425 889 275 201 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2031 3472 1537 Size: 275 219 1441 4090 Fragments arranged by size: 4090 1441 275 219 EarI CTCTTCn'nnn_ Cuts at: 1353 3955 1353 Size: 2602 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4612 4986 5132 387 Size: 1064 138 3023 374 146 1280 Fragments arranged by size: 3023 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3276 3276 Size: 6025 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2542 2587 4088 4685 5472 5740 1239 Size: 393 910 45 1501 597 787 268 1524 Fragments arranged by size: 1524 1501 910 787 597 393 268 45 EcoO109I rG'GnC_Cy Cuts at: 2043 2066 2104 2764 2854 2043 Size: 23 38 660 90 5214 Fragments arranged by size: 5214 660 90 38 23 EcoRI G'AATT_C Cuts at: 3334 3712 3334 Size: 378 5647 FspI TGC'GCA Cuts at: 4531 4531 Size: 6025 HaeII r_GCGC'y Cuts at: 1678 5172 1678 Size: 3494 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2247 2271 2273 2279 2303 Size: 32 135 32 675 24 2 6 24 Cuts at: 2303 2305 2481 2513 2771 2803 4666 4698 4740 Size: 2 176 32 258 32 1863 32 42 Cuts at: 4740 4772 1373 Size: 32 2626 Fragments arranged by size: 2626 1863 675 258 176 135 42 32 32 32 32 32 32 24 24 6 2 2 HincII GTy'rAC Cuts at: 669 1657 1938 2905 3175 3369 3615 669 Size: 988 281 967 270 194 246 3079 Fragments arranged by size: 3079 988 967 281 270 246 194 HindIII A'AGCT_T Cuts at: 748 748 Size: 6025 HpaI GTT'AAC Cuts at: 3615 3615 Size: 6025 KpnI G_GTAC'C Cuts at: 3350 3350 Size: 6025 MfeI C'AATT_G Cuts at: 3624 3624 Size: 6025 MluI A'CGCG_T Cuts at: 5901 5901 Size: 6025 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5139 5323 1305 Size: 3834 184 2007 Fragments arranged by size: 3834 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4203 Fragments arranged by size: 4203 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2325 3983 4342 4501 518 Size: 648 1159 1658 359 159 2042 Fragments arranged by size: 2042 1658 1159 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2135 2156 2582 3131 3441 4118 5266 5511 Size: 529 21 426 549 310 677 1148 245 Cuts at: 5511 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 549 529 426 310 245 21 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4534 Fragments arranged by size: 4534 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6025 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6025 NotI GC'GGCC_GC Cuts at: 3472 3472 Size: 6025 NspI r_CATG'y Cuts at: 1132 1229 2911 3383 4928 1132 Size: 97 1682 472 1545 2229 Fragments arranged by size: 2229 1682 1545 472 97 PasI CC'CwG_GG Cuts at: 1348 2070 2609 1348 Size: 722 539 4764 Fragments arranged by size: 4764 722 539 PciI A'CATG_T Cuts at: 2907 4924 2907 Size: 2017 4008 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6025 PfoI T'CCnGG_A Cuts at: 2436 2877 2436 Size: 441 5584 PmeI GTTT'AAAC Cuts at: 3329 3329 Size: 6025 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4094 4126 5607 5639 4094 Size: 32 1481 32 4480 Fragments arranged by size: 4480 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2066 2104 2764 2854 2066 Size: 38 660 90 5237 Fragments arranged by size: 5237 660 90 38 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6025 PsiI TTA'TAA Cuts at: 3595 3595 Size: 6025 PspOMI G'GGCC_C Cuts at: 2043 2043 Size: 6025 PstI C_TGCA'G Cuts at: 830 3377 830 Size: 2547 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6025 PvuII CAG'CTG Cuts at: 2156 2582 2156 Size: 426 5599 SacI G_AGCT'C Cuts at: 3278 3278 Size: 6025 SacII CC_GC'GG Cuts at: 2136 2136 Size: 6025 SalI G'TCGA_C Cuts at: 1936 3367 1936 Size: 1431 4594 SanDI GG'GwC_CC Cuts at: 2104 2764 2104 Size: 660 5365 SbfI CC_TGCA'GG Cuts at: 3377 3377 Size: 6025 ScaI AGT'ACT Cuts at: 4273 5985 4273 Size: 1712 4313 SexAI A'CCwGG_T Cuts at: 2354 2354 Size: 6025 SfcI C'TryA_G Cuts at: 826 1045 2311 3373 4508 5189 5380 826 Size: 219 1266 1062 1135 681 191 1471 Fragments arranged by size: 1471 1266 1135 1062 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6025 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6025 SmaI CCC'GGG Cuts at: 1872 2920 3352 1872 Size: 1048 432 4545 Fragments arranged by size: 4545 1048 432 SmlI C'TyrA_G Cuts at: 820 1017 1943 4129 5030 5292 820 Size: 197 926 2186 901 262 1553 Fragments arranged by size: 2186 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3816 493 Size: 3323 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6025 SphI G_CATG'C Cuts at: 1132 3383 1132 Size: 2251 3774 SspI AAT'ATT Cuts at: 5 52 3949 5 Size: 47 3897 2081 Fragments arranged by size: 3897 2081 47 StuI AGG'CCT Cuts at: 1517 2092 1517 Size: 575 5450 StyI C'CwwG_G Cuts at: 513 1065 2004 3219 3464 513 Size: 552 939 1215 245 3074 Fragments arranged by size: 3074 1215 939 552 245 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2189 2864 4052 4069 4222 4407 4852 Size: 1255 609 675 1188 17 153 185 445 Cuts at: 4852 325 Size: 1498 Fragments arranged by size: 1498 1255 1188 675 609 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2513 3150 4271 Size: 274 80 33 51 1443 536 637 1121 Cuts at: 4271 5983 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1121 637 536 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2467 2499 5985 6017 2467 Size: 32 3486 32 2475 Fragments arranged by size: 3486 2475 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2813 1316 Size: 1497 4528 XbaI T'CTAG_A Cuts at: 3295 3361 3295 Size: 66 5959 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6025 XmaI C'CCGG_G Cuts at: 1870 2918 3350 1870 Size: 1048 432 4545 Fragments arranged by size: 4545 1048 432 XmnI GAAnn'nnTTC Cuts at: 4154 4154 Size: 6025 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3833 276 Size: 53 83 186 936 2299 2468 Fragments arranged by size: 2468 2299 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SapI SfiI SfoI SrfI SwaI XcmI