(Circular) (Six-base) MAPSORT of: pFN21AB5634.seq Check: 7874 from: 1 to: 7057 pFN21AB5634 7057 bp With 182 enzymes: SgfI * February 27, 2009 14:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2072 4054 2072 Size: 1982 5075 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4867 278 Size: 53 83 186 936 3331 2468 Fragments arranged by size: 3331 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3315 4400 1937 Size: 1378 1085 4594 Fragments arranged by size: 4594 1378 1085 Acc65I G'GTAC_C Cuts at: 4378 4378 Size: 7057 AclI AA'CG_TT Cuts at: 1563 5184 5557 1563 Size: 3621 373 3063 Fragments arranged by size: 3621 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2606 2829 3167 5120 6504 6772 1632 Size: 974 223 338 1953 1384 268 1917 Fragments arranged by size: 1953 1917 1384 974 338 268 223 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6860 AflIII A'CryG_T Cuts at: 1203 2307 5956 6933 1203 Size: 1104 3649 977 1327 Fragments arranged by size: 3649 1327 1104 977 AgeI A'CCGG_T Cuts at: 5888 5888 Size: 7057 AhdI GACnn_n'nnGTC Cuts at: 5786 5786 Size: 7057 AleI CACnn'nnGTG Cuts at: 1166 2819 2831 3098 1166 Size: 1653 12 267 5125 Fragments arranged by size: 5125 1653 267 12 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3923 1184 Size: 2739 4318 AlwNI CAG_nnn'CTG Cuts at: 4800 6372 4800 Size: 1572 5485 ApaI G_GGCC'C Cuts at: 4110 4110 Size: 7057 ApaLI G'TGCA_C Cuts at: 5114 6270 5114 Size: 1156 5901 ApoI r'AATT_y Cuts at: 1493 3548 4366 4592 4744 6853 1493 Size: 2055 818 226 152 2109 1697 Fragments arranged by size: 2109 2055 1697 818 226 152 AseI AT'TA_AT Cuts at: 160 1032 5611 160 Size: 872 4579 1606 Fragments arranged by size: 4579 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7057 AvaI C'yCGr_G Cuts at: 1870 1943 2719 4109 4338 4382 1870 Size: 73 776 1390 229 44 4545 Fragments arranged by size: 4545 1390 776 229 73 44 BamHI G'GATC_C Cuts at: 4387 4754 4387 Size: 367 6690 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2039 2133 2825 3704 4244 Size: 325 217 627 252 94 692 879 540 Cuts at: 4244 4378 5833 618 Size: 134 1455 1842 Fragments arranged by size: 1842 1455 879 692 627 540 325 252 217 134 94 BanII G_rGCy'C Cuts at: 1391 2840 2868 3056 3076 3111 4110 4211 4376 Size: 1449 28 188 20 35 999 101 165 Cuts at: 4376 1391 Size: 4072 Fragments arranged by size: 4072 1449 999 188 165 101 35 28 20 BbeI G_GCGC'C Cuts at: 2043 2137 2043 Size: 94 6963 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2272 2817 928 Size: 971 373 545 5168 Fragments arranged by size: 5168 971 545 373 BbvCI CC'TCA_GC Cuts at: 4232 4232 Size: 7057 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5248 5282 1903 Size: 34 50 34 3227 34 3678 Fragments arranged by size: 3678 3227 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3215 4949 6159 3215 Size: 1734 1210 4113 Fragments arranged by size: 4113 1734 1210 BclI T'GATC_A Cuts at: 1548 3843 1548 Size: 2295 4762 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4027 4081 5668 Size: 107 122 71 631 605 2355 54 1587 Cuts at: 5668 136 Size: 1525 Fragments arranged by size: 2355 1587 1525 631 605 122 107 71 54 BglII A'GATC_T Cuts at: 3501 7052 3501 Size: 3551 3506 BlpI GC'TnA_GC Cuts at: 4317 4474 4317 Size: 157 6900 Bme1580I G_kGCm'C Cuts at: 1790 2726 2828 3707 4099 4110 4247 5118 6274 Size: 936 102 879 392 11 137 871 1156 Cuts at: 6274 1790 Size: 2573 Fragments arranged by size: 2573 1156 936 879 871 392 137 102 11 BmgBI CAC'GTC Cuts at: 1318 2308 2434 4267 1318 Size: 990 126 1833 4108 Fragments arranged by size: 4108 1833 990 126 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4300 4788 5746 Size: 416 53 83 399 24 2883 488 958 Cuts at: 5746 442 Size: 1753 Fragments arranged by size: 2883 1753 958 488 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7057 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2359 2359 Size: 7057 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2417 5717 1239 Size: 1178 3300 2579 Fragments arranged by size: 3300 2579 1178 Bpu10I CC'TnA_GC Cuts at: 4232 4232 Size: 7057 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5182 6047 6345 5182 Size: 865 298 5894 Fragments arranged by size: 5894 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2550 5720 882 Size: 617 1051 3170 2219 Fragments arranged by size: 3170 2219 1051 617 BsaAI yAC'GTr Cuts at: 493 1204 4848 493 Size: 711 3644 2702 Fragments arranged by size: 3644 2702 711 BsaBI GATnn'nnATC Cuts at: 7051 7051 Size: 7057 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2040 2134 4041 4864 Size: 53 83 186 936 507 94 1907 823 Cuts at: 4864 5246 5881 275 Size: 382 635 1451 Fragments arranged by size: 1907 1451 936 823 635 507 382 186 94 83 53 BsaWI w'CCGG_w Cuts at: 2874 3876 5490 5888 6162 6309 6720 2874 Size: 1002 1614 398 274 147 411 3211 Fragments arranged by size: 3211 1614 1002 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2253 2283 4193 4223 4337 Size: 30 165 30 850 30 1910 30 114 Cuts at: 4337 4367 1178 Size: 30 3868 Fragments arranged by size: 3868 1910 850 165 114 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2015 3959 4358 1186 Size: 829 1944 399 3885 Fragments arranged by size: 3885 1944 829 399 BseYI C'CCAG_C Cuts at: 6260 6756 6260 Size: 496 6561 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4088 4172 1163 Size: 573 2352 84 4048 Fragments arranged by size: 4048 2352 573 84 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4507 5268 6296 1540 Size: 275 186 2506 761 1028 2301 Fragments arranged by size: 2506 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3993 5118 5203 6274 3993 Size: 1125 85 1071 4776 Fragments arranged by size: 4776 1125 1071 85 BsmI GAATG_Cn' Cuts at: 2245 4568 4661 2245 Size: 2323 93 4641 Fragments arranged by size: 4641 2323 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2298 2298 Size: 7057 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2726 2828 2840 2868 3056 3076 3111 Size: 399 936 102 12 28 188 20 35 Cuts at: 3111 3707 3993 4099 4110 4211 4247 4376 5118 Size: 596 286 106 11 101 36 129 742 Cuts at: 5118 5203 6274 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 936 742 596 399 286 188 129 106 102 101 85 36 35 28 20 12 11 BspEI T'CCGG_A Cuts at: 3876 6720 3876 Size: 2844 4213 BspHI T'CATG_A Cuts at: 2016 2428 4946 6676 2016 Size: 412 2518 1730 2397 Fragments arranged by size: 2518 2397 1730 412 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2072 2259 4054 4412 844 Size: 1228 187 1795 358 3489 Fragments arranged by size: 3489 1795 1228 358 187 BsrBI CCG'CTC Cuts at: 1394 1823 4944 1394 Size: 429 3121 3507 Fragments arranged by size: 3507 3121 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5552 5726 65 Size: 1183 4304 174 1396 Fragments arranged by size: 4304 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2130 5701 5888 1700 Size: 110 320 3571 187 2869 Fragments arranged by size: 3571 2869 320 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7057 BssHII G'CGCG_C Cuts at: 1925 3891 1925 Size: 1966 5091 BssSI C'ACGA_G Cuts at: 3920 5117 6129 3920 Size: 1197 1012 4848 Fragments arranged by size: 4848 1197 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2084 2534 794 Size: 1290 450 5317 Fragments arranged by size: 5317 1290 450 BstBI TT'CG_AA Cuts at: 5861 5861 Size: 7057 BstEII G'GTnAC_C Cuts at: 1184 3931 1184 Size: 2747 4310 BstXI CCAn_nnnn'nTGG Cuts at: 2737 3352 2737 Size: 615 6442 BstYI r'GATC_y Cuts at: 1972 3501 3915 4387 4754 5139 5156 6608 6694 Size: 1529 414 472 367 385 17 1452 86 Cuts at: 6694 6706 7052 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1529 1452 472 414 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3060 3189 3481 3666 3720 4006 4025 Size: 552 1995 129 292 185 54 286 19 Cuts at: 4025 513 Size: 3545 Fragments arranged by size: 3545 1995 552 292 286 185 129 54 19 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6318 Fragments arranged by size: 6318 683 56 BtsI GCAGTG_nn' Cuts at: 2067 2076 2358 2535 3810 4573 5367 5387 2067 Size: 9 282 177 1275 763 794 20 3737 Fragments arranged by size: 3737 1275 794 763 282 177 20 9 ClaI AT'CG_AT Cuts at: 1554 2610 1554 Size: 1056 6001 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7022 DraI TTT'AAA Cuts at: 4361 4361 Size: 7057 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3311 6064 809 Size: 507 1995 2753 1802 Fragments arranged by size: 2753 1995 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2727 3186 3484 Size: 54 1425 50 275 18 897 459 298 Cuts at: 3484 3603 3723 3774 4504 5393 7022 8 Size: 119 120 51 730 889 1629 43 Fragments arranged by size: 1629 1425 897 889 730 459 298 275 120 119 54 51 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4504 1537 Size: 275 2692 4090 Fragments arranged by size: 4090 2692 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2172 4987 1353 Size: 819 2815 3423 Fragments arranged by size: 3423 2815 819 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4166 5644 6018 6164 387 Size: 1064 138 2577 1478 374 146 1280 Fragments arranged by size: 2577 1478 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2417 2606 2829 3167 5120 5717 6504 Size: 393 785 189 223 338 1953 597 787 Cuts at: 6504 6772 1239 Size: 268 1524 Fragments arranged by size: 1953 1524 787 785 597 393 338 268 223 189 EcoO109I rG'GnC_Cy Cuts at: 2505 2704 2854 4106 4295 2505 Size: 199 150 1252 189 5267 Fragments arranged by size: 5267 1252 199 189 150 EcoRI G'AATT_C Cuts at: 4366 4744 4366 Size: 378 6679 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3673 3673 Size: 7057 FspI TGC'GCA Cuts at: 4062 5563 4062 Size: 1501 5556 HaeII r_GCGC'y Cuts at: 1678 2043 2137 6204 1678 Size: 365 94 4067 2531 Fragments arranged by size: 4067 2531 365 94 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2549 2581 2613 2645 3063 Size: 32 135 32 977 32 32 32 418 Cuts at: 3063 3095 3213 3245 3679 3711 5698 5730 5772 Size: 32 118 32 434 32 1987 32 42 Cuts at: 5772 5804 1373 Size: 32 2626 Fragments arranged by size: 2626 1987 977 434 418 135 118 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4401 4647 669 Size: 988 281 2463 246 3079 Fragments arranged by size: 3079 2463 988 281 246 HindIII A'AGCT_T Cuts at: 748 2644 748 Size: 1896 5161 HpaI GTT'AAC Cuts at: 4647 4647 Size: 7057 KasI G'GCGC_C Cuts at: 2039 2133 2039 Size: 94 6963 KpnI G_GTAC'C Cuts at: 4382 4382 Size: 7057 MfeI C'AATT_G Cuts at: 4656 4656 Size: 7057 MluI A'CGCG_T Cuts at: 6933 6933 Size: 7057 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4281 6171 6355 1305 Size: 2976 1890 184 2007 Fragments arranged by size: 2976 2007 1890 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3188 3486 10 Size: 54 1425 343 1356 298 3581 Fragments arranged by size: 3581 1425 1356 343 298 54 MslI CAynn'nnrTG Cuts at: 518 1166 2172 2519 2819 2831 3098 3839 5015 Size: 648 1006 347 300 12 267 741 1176 Cuts at: 5015 5374 5533 518 Size: 359 159 2042 Fragments arranged by size: 2042 1176 1006 741 648 359 347 300 267 159 12 MspA1I CmG'CkG Cuts at: 1606 3062 3145 3490 3928 4008 4027 4473 5150 Size: 1456 83 345 438 80 19 446 677 Cuts at: 5150 6298 6543 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1456 1148 677 446 438 345 245 83 80 19 NarI GG'CG_CC Cuts at: 2040 2134 2040 Size: 94 6963 NcoI C'CATG_G Cuts at: 513 1065 3189 3481 513 Size: 552 2124 292 4089 Fragments arranged by size: 4089 2124 552 292 NdeI CA'TA_TG Cuts at: 387 387 Size: 7057 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7057 NotI GC'GGCC_GC Cuts at: 4504 4504 Size: 7057 NspI r_CATG'y Cuts at: 1132 1229 4415 5960 1132 Size: 97 3186 1545 2229 Fragments arranged by size: 3186 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2701 4240 1348 Size: 1353 1539 4165 Fragments arranged by size: 4165 1539 1353 PciI A'CATG_T Cuts at: 5956 5956 Size: 7057 PflMI CCAn_nnn'nTGG Cuts at: 1108 3805 1108 Size: 2697 4360 PmeI GTTT'AAAC Cuts at: 4361 4361 Size: 7057 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5126 5158 6639 6671 5126 Size: 32 1481 32 5512 Fragments arranged by size: 5512 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2505 2854 4295 2505 Size: 349 1441 5267 Fragments arranged by size: 5267 1441 349 PshAI GACnn'nnGTC Cuts at: 1613 2510 1613 Size: 897 6160 PsiI TTA'TAA Cuts at: 4627 4627 Size: 7057 PspOMI G'GGCC_C Cuts at: 4106 4106 Size: 7057 PstI C_TGCA'G Cuts at: 830 3501 4409 830 Size: 2671 908 3478 Fragments arranged by size: 3478 2671 908 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7057 PvuII CAG'CTG Cuts at: 3145 3490 3928 3145 Size: 345 438 6274 Fragments arranged by size: 6274 438 345 SacII CC_GC'GG Cuts at: 3063 4009 4028 3063 Size: 946 19 6092 Fragments arranged by size: 6092 946 19 SalI G'TCGA_C Cuts at: 1936 4399 1936 Size: 2463 4594 SanDI GG'GwC_CC Cuts at: 4295 4295 Size: 7057 SbfI CC_TGCA'GG Cuts at: 4409 4409 Size: 7057 ScaI AGT'ACT Cuts at: 5305 7017 5305 Size: 1712 5345 SfcI C'TryA_G Cuts at: 826 1045 3497 4405 5540 6221 6412 826 Size: 219 2452 908 1135 681 191 1471 Fragments arranged by size: 2452 1471 1135 908 681 219 191 SfoI GGC'GCC Cuts at: 2041 2135 2041 Size: 94 6963 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7057 SgrAI Cr'CCGG_yG Cuts at: 1700 2130 1700 Size: 430 6627 SmaI CCC'GGG Cuts at: 1872 4111 4340 4384 1872 Size: 2239 229 44 4545 Fragments arranged by size: 4545 2239 229 44 SmlI C'TyrA_G Cuts at: 820 1017 1943 5161 6062 6324 820 Size: 197 926 3218 901 262 1553 Fragments arranged by size: 3218 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4848 493 Size: 4355 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7057 SphI G_CATG'C Cuts at: 1132 4415 1132 Size: 3283 3774 SspI AAT'ATT Cuts at: 5 52 4981 5 Size: 47 4929 2081 Fragments arranged by size: 4929 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7057 StyI C'CwwG_G Cuts at: 513 1065 3189 3481 3648 4496 513 Size: 552 2124 292 167 848 3074 Fragments arranged by size: 3074 2124 848 552 292 167 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2123 2141 2667 2795 5084 5101 5254 Size: 1255 543 18 526 128 2289 17 153 Cuts at: 5254 5439 5884 325 Size: 185 445 1498 Fragments arranged by size: 2289 1498 1255 543 526 445 185 153 128 18 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2790 2886 5303 Size: 274 80 33 51 1443 813 96 2417 Cuts at: 5303 7015 96 Size: 1712 138 Fragments arranged by size: 2417 1712 1443 813 274 138 96 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2179 2211 2253 2285 7017 7049 2179 Size: 32 42 32 4732 32 2187 Fragments arranged by size: 4732 2187 42 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7057 XbaI T'CTAG_A Cuts at: 4393 4393 Size: 7057 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2323 2974 2323 Size: 651 6406 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7057 XmaI C'CCGG_G Cuts at: 1870 4109 4338 4382 1870 Size: 2239 229 44 4545 Fragments arranged by size: 4545 2239 229 44 XmnI GAAnn'nnTTC Cuts at: 5186 5186 Size: 7057 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4865 276 Size: 53 83 186 936 3331 2468 Fragments arranged by size: 3331 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SapI SexAI SfiI SrfI SwaI