Restriction map for
pFN21AB5633
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# NO CUT ENZYMES ( 123 enzymes) #
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AatII Acc65I AccI AccII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI ApaI ApaLI AscI
Asp718I AspEI AspI AviII AvrII
BanI BbeI BbrPI BbsI BbvCI
BcgI BciVI BfrI BglI BglII
BlnI BpuAI BsaAI BsaBI BsaHI
BsgI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspHI BspLU11I BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraI DraII
DrdI EagI Eam1105I EclXI Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV FseI FspI Hin1I HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PstI PvuI RcaI
RsrII SacII SalI SapI SbfI
SexAI SfiI SfoI SgrAI SnaBI
SpeI Sse8387I SspBI StuI SwaI
Tth111I Van91I XhoI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccIII 875 TCCGGA T^CCGGA
AlwNI 72 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 520 ATTAAT AT^TAAT
AsnI 520 ATTAAT AT^TAAT
Asp700I 889 GAAACTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 985 GAGCTC G[AT]GC[AT]^C
AvaI 1153 CCCGGG C^[CT]CG[GA]G
AvaII 873 GGTCC G^G[AT]CC
BamHI 31 GGATCC G^GATCC
BanII 985 GAGCTC G[GA]GC[CT]^C
BclI 1305 TGATCA T^GATCA
BfaI 697 CTAG C^TAG
BlpI 1198 GCTCAGC GC^T[ACGT]AGC
BmyI 985 GAGCTC G[AGT]GC[ACT]^C
BpmI 135 CTCCAG CTGGAG(16/14)
Bpu1102I 1198 GCTCAGC GC^T[ACGT]AGC
BsaI 1494 GGTCTC GGTCTC(1/5)
BsaWI 875 TCCGGA [AT]^CCGG[AT]
BseAI 875 TCCGGA T^CCGGA
BsiHKAI 985 GAGCTC G[AT]GC[AT]^C
BsoBI 1153 CCCGGG C^[CT]CG[GA]G
Bsp1286I 985 GAGCTC G[AGT]GC[ACT]^C
BspEI 875 TCCGGA T^CCGGA
BspMI 301 GCAGGT ACCTGC(4/8)
BsrDI 1046 GCAATG GCAATG(2/0)
BstAPI 269 GCAAAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1001 TTCGAA TT^CGAA
CelII 1198 GCTCAGC GC^T[ACGT]AGC
DraIII 458 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
FauI 1502 CCCGC CCCGC(4/6)
FbaI 1305 TGATCA T^GATCA
HgaI 498 GCGTC GACGC(5/10)
HincII 319 GTTGAC GT[CT]^[GA]AC
HindII 319 GTTGAC GT[CT]^[GA]AC
MaeI 697 CTAG C^TAG
MaeII 372 ACGT A^CGT
MroI 875 TCCGGA T^CCGGA
MspA1I 24 CAGCTG C[AC]G^C[GT]G
NcoI 65 CCATGG C^CATGG
NspI 1169 GCATGC [GA]CATG^[CT]
NspV 1001 TTCGAA TT^CGAA
PshBI 520 ATTAAT AT^TAAT
PvuII 24 CAGCTG CAG^CTG
SacI 985 GAGCTC GAGCT^C
SfuI 1001 TTCGAA TT^CGAA
SmaI 1153 CCCGGG CCC^GGG
SphI 1169 GCATGC GCATG^C
SspI 523 AATATT AAT^ATT
XbaI 696 TCTAGA T^CTAGA
XmaCI 1153 CCCGGG C^CCGGG
XmaI 1153 CCCGGG C^CCGGG
XmnI 889 GAAACTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 28 enzymes) #
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AciI 1503 CCGC CCGC(-3/-1)
254 GCGG
Aor51HI 82 AGCGCT AGC^GCT
1202 AGCGCT
BalI 62 TGGCCA TGG^CCA
68 TGGCCA
BbvI 577 GCTGC GCAGC(8/12)
1365 GCTGC
BseRI 151 GAGGAG GAGGAG(10/8)
1105 GAGGAG
BsmFI 485 GTCCC GGGAC(10/14)
500 GTCCC
BsmI 205 GAATGC GAATGC(1/-1)
1082 GAATGC
DsaI 65 CCATGG C^C[GA][CT]GG
1393 CCACGG
EarI 1102 GAAGAG CTCTTC(1/4)
1339 GAAGAG
Eco47III 82 AGCGCT AGC^GCT
1202 AGCGCT
EcoT22I 1084 ATGCAT ATGCA^T
1429 ATGCAT
HaeII 82 AGCGCT [GA]GCGC^[CT]
1202 AGCGCT
HphI 393 GGTGA GGTGA(8/7)
897 TCACC
Ksp632I 1102 GAAGAG CTCTTC(1/4)
1339 GAAGAG
MluNI 62 TGGCCA TGG^CCA
68 TGGCCA
MscI 62 TGGCCA TGG^CCA
68 TGGCCA
MseI 521 TTAA T^TAA
1463 TTAA
MslI 807 CATGAGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1037 CACAGCAGTG
NsiI 1084 ATGCAT ATGCA^T
1429 ATGCAT
PleI 853 GACTC GAGTC(4/5)
3 GAGTC
ScaI 733 AGTACT AGT^ACT
1549 AGTACT
SfcI 78 CTGTAG C^T[GA][CT]AG
707 CTACAG
SmlI 442 CTTGAG C^T[CT][GA]AG
1009 CTTGAG
TaqI 1002 TCGA T^CGA
1031 TCGA
Tru9I 521 TTAA T^TAA
1463 TTAA
TseI 577 GCTGC G^C[AT]GC
1365 GCTGC
TthHB8I 1002 TCGA T^CGA
1031 TCGA
XcmI 137 CCAGCTCATTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
212 CCATCTGCTTGATGG
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# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
BcnI BsaJI BsiYI BslI BsmAI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
EaeI EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII ItaI MaeIII MboI MboII
MflI MnlI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfaNI StyI
TfiI Tsp45I Tsp509I TspRI XhoII