(Circular) (Six-base) MAPSORT of: pFN21AB5580.seq Check: 1542 from: 1 to: 6550 pFN21AB5580 6550 bp With 182 enzymes: SgfI * October 20, 2009 10:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3420 3420 Size: 6550 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2385 4360 278 Size: 53 83 186 936 849 1975 2468 Fragments arranged by size: 2468 1975 936 849 186 83 53 AccI GT'mk_AC Cuts at: 1937 3113 3893 1937 Size: 1176 780 4594 Fragments arranged by size: 4594 1176 780 Acc65I G'GTAC_C Cuts at: 3871 3871 Size: 6550 AclI AA'CG_TT Cuts at: 1563 4677 5050 1563 Size: 3114 373 3063 Fragments arranged by size: 3114 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2934 4613 5997 6265 1632 Size: 1302 1679 1384 268 1917 Fragments arranged by size: 1917 1679 1384 1302 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6353 AflIII A'CryG_T Cuts at: 1203 3157 5449 6426 1203 Size: 1954 2292 977 1327 Fragments arranged by size: 2292 1954 1327 977 AgeI A'CCGG_T Cuts at: 5381 5381 Size: 6550 AhdI GACnn_n'nnGTC Cuts at: 5279 5279 Size: 6550 AleI CACnn'nnGTG Cuts at: 1166 2180 2741 1166 Size: 1014 561 4975 Fragments arranged by size: 4975 1014 561 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6550 AlwNI CAG_nnn'CTG Cuts at: 4293 5865 4293 Size: 1572 4978 ApaLI G'TGCA_C Cuts at: 3209 4607 5763 3209 Size: 1398 1156 3996 Fragments arranged by size: 3996 1398 1156 ApoI r'AATT_y Cuts at: 1493 2503 3608 3859 4085 4237 6346 1493 Size: 1010 1105 251 226 152 2109 1697 Fragments arranged by size: 2109 1697 1105 1010 251 226 152 AseI AT'TA_AT Cuts at: 160 1032 5104 160 Size: 872 4072 1606 Fragments arranged by size: 4072 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6550 AvaI C'yCGr_G Cuts at: 1870 1943 2991 3875 1870 Size: 73 1048 884 4545 Fragments arranged by size: 4545 1048 884 73 BamHI G'GATC_C Cuts at: 2195 2328 3687 3880 4247 2195 Size: 133 1359 193 367 4498 Fragments arranged by size: 4498 1359 367 193 133 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3871 5326 618 Size: 325 217 627 2084 1455 1842 Fragments arranged by size: 2084 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2081 2881 3265 3339 3725 3869 1391 Size: 690 800 384 74 386 144 4072 Fragments arranged by size: 4072 800 690 386 384 144 74 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2381 2768 3672 928 Size: 971 482 387 904 3806 Fragments arranged by size: 3806 971 904 482 387 BbvCI CC'TCA_GC Cuts at: 2064 2064 Size: 6550 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3245 3279 4741 4775 1903 Size: 34 1308 34 1462 34 3678 Fragments arranged by size: 3678 1462 1308 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4442 5652 4442 Size: 1210 5340 BclI T'GATC_A Cuts at: 1548 1548 Size: 6550 BfrBI ATG'CAT Cuts at: 3539 3539 Size: 6550 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3284 5161 136 Size: 107 122 71 631 605 1612 1877 1525 Fragments arranged by size: 1877 1612 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6545 6545 Size: 6550 BlpI GC'TnA_GC Cuts at: 3967 3967 Size: 6550 Bme1580I G_kGCm'C Cuts at: 1790 2888 3213 3367 4611 5767 1790 Size: 1098 325 154 1244 1156 2573 Fragments arranged by size: 2573 1244 1156 1098 325 154 BmgBI CAC'GTC Cuts at: 1318 3094 3160 1318 Size: 1776 66 4708 Fragments arranged by size: 4708 1776 66 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2253 2378 3177 Size: 416 53 83 399 24 836 125 799 Cuts at: 3177 3268 3352 4281 5239 442 Size: 91 84 929 958 1753 Fragments arranged by size: 1753 958 929 836 799 416 399 125 91 84 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6550 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5210 1239 Size: 3971 2579 Bpu10I CC'TnA_GC Cuts at: 2064 3007 2064 Size: 943 5607 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3785 4675 5540 5838 3785 Size: 890 865 298 4497 Fragments arranged by size: 4497 890 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3099 3761 5213 882 Size: 617 1600 662 1452 2219 Fragments arranged by size: 2219 1600 1452 662 617 BsaAI yAC'GTr Cuts at: 493 1204 2822 4341 493 Size: 711 1618 1519 2702 Fragments arranged by size: 2702 1618 1519 711 BsaBI GATnn'nnATC Cuts at: 6544 6544 Size: 6550 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2187 2382 4357 4739 Size: 53 83 186 936 654 195 1975 382 Cuts at: 4739 5374 275 Size: 635 1451 Fragments arranged by size: 1975 1451 936 654 635 382 195 186 83 53 BsaWI w'CCGG_w Cuts at: 2192 2556 2700 3450 3836 4983 5381 5655 5802 Size: 364 144 750 386 1147 398 274 147 Cuts at: 5802 6213 2192 Size: 411 2529 Fragments arranged by size: 2529 1147 750 411 398 386 364 274 147 144 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2417 2447 1178 Size: 30 165 30 1014 30 5281 Fragments arranged by size: 5281 1014 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3028 3093 1186 Size: 1842 65 4643 Fragments arranged by size: 4643 1842 65 BseYI C'CCAG_C Cuts at: 3274 5753 6249 3274 Size: 2479 496 3575 Fragments arranged by size: 3575 2479 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2642 1163 Size: 573 906 5071 Fragments arranged by size: 5071 906 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2440 4000 4761 5789 1540 Size: 275 186 439 1560 761 1028 2301 Fragments arranged by size: 2301 1560 1028 761 439 275 186 BsiHKAI G_wGCw'C Cuts at: 2881 3213 3339 3474 3725 4611 4696 5767 2881 Size: 332 126 135 251 886 85 1071 3664 Fragments arranged by size: 3664 1071 886 332 251 135 126 85 BsmI GAATG_Cn' Cuts at: 2070 4061 4154 2070 Size: 1991 93 4466 Fragments arranged by size: 4466 1991 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2081 2881 2888 3213 3265 3339 3367 Size: 399 291 800 7 325 52 74 28 Cuts at: 3367 3474 3725 3869 4611 4696 5767 1391 Size: 107 251 144 742 85 1071 2174 Fragments arranged by size: 2174 1071 800 742 399 325 291 251 144 107 85 74 52 28 7 BspEI T'CCGG_A Cuts at: 2192 2700 6213 2192 Size: 508 3513 2529 Fragments arranged by size: 3513 2529 508 BspHI T'CATG_A Cuts at: 3372 4439 6169 3372 Size: 1067 1730 3753 Fragments arranged by size: 3753 1730 1067 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2095 3420 3905 844 Size: 1251 1325 485 3489 Fragments arranged by size: 3489 1325 1251 485 BsrBI CCG'CTC Cuts at: 1394 1823 4437 1394 Size: 429 2614 3507 Fragments arranged by size: 3507 2614 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3772 5045 5219 65 Size: 1183 2524 1273 174 1396 Fragments arranged by size: 2524 1396 1273 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2577 3703 5194 5381 1700 Size: 110 767 1126 1491 187 2869 Fragments arranged by size: 2869 1491 1126 767 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6550 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6550 BssSI C'ACGA_G Cuts at: 4610 5622 4610 Size: 1012 5538 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6550 BstBI TT'CG_AA Cuts at: 5354 5354 Size: 6550 BstEII G'GTnAC_C Cuts at: 1184 3446 1184 Size: 2262 4288 BstXI CCAn_nnnn'nTGG Cuts at: 2743 3391 2743 Size: 648 5902 BstYI r'GATC_y Cuts at: 1972 2195 2328 3687 3880 4247 4632 4649 6101 Size: 223 133 1359 193 367 385 17 1452 Cuts at: 6101 6187 6199 6545 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1359 385 367 346 223 193 133 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2847 513 Size: 552 1782 4216 Fragments arranged by size: 4216 1782 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5811 Fragments arranged by size: 5811 683 56 BtsI GCAGTG_nn' Cuts at: 2187 4066 4860 4880 2187 Size: 1879 794 20 3857 Fragments arranged by size: 3857 1879 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6550 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6515 DraI TTT'AAA Cuts at: 3854 3854 Size: 6550 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2315 2772 3158 5557 809 Size: 507 999 457 386 2399 1802 Fragments arranged by size: 2399 1802 999 507 457 386 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2437 2844 3826 Size: 54 1425 50 275 18 607 407 982 Cuts at: 3826 3997 4886 6515 8 Size: 171 889 1629 43 Fragments arranged by size: 1629 1425 982 889 607 407 275 171 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2437 3997 1537 Size: 275 625 1560 4090 Fragments arranged by size: 4090 1560 625 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3046 3344 4480 1353 Size: 1693 298 1136 3423 Fragments arranged by size: 3423 1693 1136 298 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5137 5511 5657 387 Size: 1064 138 3548 374 146 1280 Fragments arranged by size: 3548 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2879 3337 3723 2879 Size: 458 386 5706 Fragments arranged by size: 5706 458 386 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2934 4613 5210 5997 6265 1239 Size: 393 1302 1679 597 787 268 1524 Fragments arranged by size: 1679 1524 1302 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 3137 3137 Size: 6550 EcoRI G'AATT_C Cuts at: 3859 4237 3859 Size: 378 6172 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2946 3455 2946 Size: 509 6041 FspI TGC'GCA Cuts at: 5056 5056 Size: 6550 HaeII r_GCGC'y Cuts at: 1678 2283 5697 1678 Size: 605 3414 2531 Fragments arranged by size: 3414 2531 605 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2148 2180 2336 2368 2417 Size: 32 135 32 576 32 156 32 49 Cuts at: 2417 2449 2716 2748 2957 2989 3023 3055 3072 Size: 32 267 32 209 32 34 32 17 Cuts at: 3072 3104 3215 3247 5191 5223 5265 5297 1373 Size: 32 111 32 1944 32 42 32 2626 Fragments arranged by size: 2626 1944 576 267 209 156 135 111 49 42 34 32 32 32 32 32 32 32 32 32 32 32 32 17 HincII GTy'rAC Cuts at: 669 1657 1938 3114 3406 3894 4140 669 Size: 988 281 1176 292 488 246 3079 Fragments arranged by size: 3079 1176 988 488 292 281 246 HindIII A'AGCT_T Cuts at: 748 3049 748 Size: 2301 4249 HpaI GTT'AAC Cuts at: 4140 4140 Size: 6550 KpnI G_GTAC'C Cuts at: 3875 3875 Size: 6550 MfeI C'AATT_G Cuts at: 2668 4149 2668 Size: 1481 5069 MluI A'CGCG_T Cuts at: 6426 6426 Size: 6550 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3750 5664 5848 1305 Size: 2445 1914 184 2007 Fragments arranged by size: 2445 2007 1914 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2846 3828 10 Size: 54 1425 343 1014 982 2732 Fragments arranged by size: 2732 1425 1014 982 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2180 2609 2741 4508 4867 5026 518 Size: 648 1014 429 132 1767 359 159 2042 Fragments arranged by size: 2042 1767 1014 648 429 359 159 132 MspA1I CmG'CkG Cuts at: 1606 2389 2517 2565 3966 4643 5791 6036 1606 Size: 783 128 48 1401 677 1148 245 2120 Fragments arranged by size: 2120 1401 1148 783 677 245 128 48 NaeI GCC'GGC Cuts at: 2579 3705 2579 Size: 1126 5424 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5998 NdeI CA'TA_TG Cuts at: 387 387 Size: 6550 NgoMIV G'CCGG_C Cuts at: 2577 3703 2577 Size: 1126 5424 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6550 NotI GC'GGCC_GC Cuts at: 3997 3997 Size: 6550 NsiI A_TGCA'T Cuts at: 3541 3541 Size: 6550 NspI r_CATG'y Cuts at: 1132 1229 3908 5453 1132 Size: 97 2679 1545 2229 Fragments arranged by size: 2679 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6550 PciI A'CATG_T Cuts at: 5449 5449 Size: 6550 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6550 PfoI T'CCnGG_A Cuts at: 3426 3426 Size: 6550 PmeI GTTT'AAAC Cuts at: 3854 3854 Size: 6550 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4619 4651 6132 6164 4619 Size: 32 1481 32 5005 Fragments arranged by size: 5005 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2381 1613 Size: 768 5782 PsiI TTA'TAA Cuts at: 4120 4120 Size: 6550 PstI C_TGCA'G Cuts at: 830 3417 3489 3902 830 Size: 2587 72 413 3478 Fragments arranged by size: 3478 2587 413 72 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6550 PvuII CAG'CTG Cuts at: 2517 2517 Size: 6550 SacI G_AGCT'C Cuts at: 2881 3339 3725 2881 Size: 458 386 5706 Fragments arranged by size: 5706 458 386 SalI G'TCGA_C Cuts at: 1936 3112 3892 1936 Size: 1176 780 4594 Fragments arranged by size: 4594 1176 780 SapI GCTCTTCn'nnn_ Cuts at: 3344 3344 Size: 6550 SbfI CC_TGCA'GG Cuts at: 3902 3902 Size: 6550 ScaI AGT'ACT Cuts at: 2408 4798 6510 2408 Size: 2390 1712 2448 Fragments arranged by size: 2448 2390 1712 SfcI C'TryA_G Cuts at: 826 1045 2052 2512 3413 3485 3552 3898 5033 Size: 219 1007 460 901 72 67 346 1135 Cuts at: 5033 5714 5905 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1007 901 681 460 346 219 191 72 67 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6550 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6550 SmaI CCC'GGG Cuts at: 1872 3877 1872 Size: 2005 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3800 4654 5555 5817 820 Size: 197 926 1857 854 901 262 1553 Fragments arranged by size: 1857 1553 926 901 854 262 197 SnaBI TAC'GTA Cuts at: 493 4341 493 Size: 3848 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6550 SphI G_CATG'C Cuts at: 1132 3908 1132 Size: 2776 3774 SspI AAT'ATT Cuts at: 5 52 4474 5 Size: 47 4422 2081 Fragments arranged by size: 4422 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6550 StyI C'CwwG_G Cuts at: 513 1065 3989 513 Size: 552 2924 3074 Fragments arranged by size: 3074 2924 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3133 3255 4577 4594 4747 4932 5377 Size: 1255 1553 122 1322 17 153 185 445 Cuts at: 5377 325 Size: 1498 Fragments arranged by size: 1553 1498 1322 1255 445 185 153 122 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2406 4796 6508 Size: 274 80 33 51 1443 429 2390 1712 Cuts at: 6508 96 Size: 138 Fragments arranged by size: 2390 1712 1443 429 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6510 6542 6510 Size: 32 6518 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6550 XbaI T'CTAG_A Cuts at: 3886 3886 Size: 6550 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6550 XmaI C'CCGG_G Cuts at: 1870 3875 1870 Size: 2005 4545 XmnI GAAnn'nnTTC Cuts at: 2639 4679 2639 Size: 2040 4510 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2383 4358 276 Size: 53 83 186 936 849 1975 2468 Fragments arranged by size: 2468 1975 936 849 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NarI NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI