(Circular) (Six-base) MAPSORT of: pFN21AB5556.seq Check: 4910 from: 1 to: 5698 pFN21AB5556 5698 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2643 2643 Size: 5698 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3508 278 Size: 53 83 186 936 1972 2468 Fragments arranged by size: 2468 1972 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2511 3041 1937 Size: 574 530 4594 Fragments arranged by size: 4594 574 530 Acc65I G'GTAC_C Cuts at: 3019 3019 Size: 5698 AclI AA'CG_TT Cuts at: 1563 3825 4198 1563 Size: 2262 373 3063 Fragments arranged by size: 3063 2262 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2171 2464 2699 3761 5145 5413 1632 Size: 539 293 235 1062 1384 268 1917 Fragments arranged by size: 1917 1384 1062 539 293 268 235 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5501 AflIII A'CryG_T Cuts at: 1203 4597 5574 1203 Size: 3394 977 1327 Fragments arranged by size: 3394 1327 977 AgeI A'CCGG_T Cuts at: 4529 4529 Size: 5698 AhdI GACnn_n'nnGTC Cuts at: 2746 4427 2746 Size: 1681 4017 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5698 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5698 AlwNI CAG_nnn'CTG Cuts at: 2296 2677 3441 5013 2296 Size: 381 764 1572 2981 Fragments arranged by size: 2981 1572 764 381 ApaLI G'TGCA_C Cuts at: 3755 4911 3755 Size: 1156 4542 ApoI r'AATT_y Cuts at: 1493 3007 3233 3385 5494 1493 Size: 1514 226 152 2109 1697 Fragments arranged by size: 2109 1697 1514 226 152 AseI AT'TA_AT Cuts at: 160 1032 4252 160 Size: 872 3220 1606 Fragments arranged by size: 3220 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5698 AvaI C'yCGr_G Cuts at: 1870 1943 2225 2702 3023 1870 Size: 73 282 477 321 4545 Fragments arranged by size: 4545 477 321 282 73 BamHI G'GATC_C Cuts at: 3028 3395 3028 Size: 367 5331 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2563 2961 3019 4474 618 Size: 325 217 627 776 398 58 1455 1842 Fragments arranged by size: 1842 1455 776 627 398 325 217 58 BanII G_rGCy'C Cuts at: 1391 3017 1391 Size: 1626 4072 BbeI G_GCGC'C Cuts at: 2567 2567 Size: 5698 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4727 BbvCI CC'TCA_GC Cuts at: 2522 2522 Size: 5698 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3889 3923 1903 Size: 34 1952 34 3678 Fragments arranged by size: 3678 1952 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3590 4800 3590 Size: 1210 4488 BclI T'GATC_A Cuts at: 1548 1548 Size: 5698 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2453 2588 4309 Size: 107 122 71 631 605 781 135 1721 Cuts at: 4309 136 Size: 1525 Fragments arranged by size: 1721 1525 781 631 605 135 122 107 71 BglII A'GATC_T Cuts at: 5693 5693 Size: 5698 BlpI GC'TnA_GC Cuts at: 2546 3115 2546 Size: 569 5129 Bme1580I G_kGCm'C Cuts at: 1790 2265 2303 2703 3759 4915 1790 Size: 475 38 400 1056 1156 2573 Fragments arranged by size: 2573 1156 1056 475 400 38 BmgBI CAC'GTC Cuts at: 1318 2200 2397 1318 Size: 882 197 4619 Fragments arranged by size: 4619 882 197 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2132 2284 3429 Size: 416 53 83 399 24 715 152 1145 Cuts at: 3429 4387 442 Size: 958 1753 Fragments arranged by size: 1753 1145 958 715 416 399 152 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5698 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2252 2846 2864 4358 1239 Size: 1013 594 18 1494 2579 Fragments arranged by size: 2579 1494 1013 594 18 Bpu10I CC'TnA_GC Cuts at: 2522 2906 2522 Size: 384 5314 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2195 3823 4688 4986 2195 Size: 1628 865 298 2907 Fragments arranged by size: 2907 1628 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4361 882 Size: 617 2862 2219 Fragments arranged by size: 2862 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3489 493 Size: 711 2285 2702 Fragments arranged by size: 2702 2285 711 BsaBI GATnn'nnATC Cuts at: 5692 5692 Size: 5698 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2219 2564 2888 3505 Size: 53 83 186 936 686 345 324 617 Cuts at: 3505 3887 4522 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 686 635 617 382 345 324 186 83 53 BsaWI w'CCGG_w Cuts at: 4131 4529 4803 4950 5361 4131 Size: 398 274 147 411 4468 Fragments arranged by size: 4468 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2873 2903 1178 Size: 30 165 30 1470 30 3973 Fragments arranged by size: 3973 1470 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2174 2699 1186 Size: 988 525 4185 Fragments arranged by size: 4185 988 525 BseYI C'CCAG_C Cuts at: 2387 2607 2640 4901 5397 2387 Size: 220 33 2261 496 2688 Fragments arranged by size: 2688 2261 496 220 33 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2351 2477 2675 1163 Size: 573 615 126 198 4186 Fragments arranged by size: 4186 615 573 198 126 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2583 3148 3909 4937 1540 Size: 275 186 582 565 761 1028 2301 Fragments arranged by size: 2301 1028 761 582 565 275 186 BsiHKAI G_wGCw'C Cuts at: 3759 3844 4915 3759 Size: 85 1071 4542 Fragments arranged by size: 4542 1071 85 BsmI GAATG_Cn' Cuts at: 3209 3302 3209 Size: 93 5605 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2265 2303 2703 3017 3759 3844 4915 Size: 399 475 38 400 314 742 85 1071 Cuts at: 4915 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 475 400 399 314 85 38 BspEI T'CCGG_A Cuts at: 5361 5361 Size: 5698 BspHI T'CATG_A Cuts at: 2940 3587 5317 2940 Size: 647 1730 3321 Fragments arranged by size: 3321 1730 647 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2643 3053 844 Size: 1799 410 3489 Fragments arranged by size: 3489 1799 410 BsrBI CCG'CTC Cuts at: 1394 1823 2073 2758 3585 1394 Size: 429 250 685 827 3507 Fragments arranged by size: 3507 827 685 429 250 BsrDI GCAATG_nn' Cuts at: 65 1248 4193 4367 65 Size: 1183 2945 174 1396 Fragments arranged by size: 2945 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2410 2506 2582 4342 4529 1700 Size: 110 600 96 76 1760 187 2869 Fragments arranged by size: 2869 1760 600 187 110 96 76 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5698 BssHII G'CGCG_C Cuts at: 1925 2350 2541 1925 Size: 425 191 5082 Fragments arranged by size: 5082 425 191 BssSI C'ACGA_G Cuts at: 3758 4770 3758 Size: 1012 4686 BstAPI GCAn_nnn'nTGC Cuts at: 794 2635 2986 794 Size: 1841 351 3506 Fragments arranged by size: 3506 1841 351 BstBI TT'CG_AA Cuts at: 4502 4502 Size: 5698 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5698 BstXI CCAn_nnnn'nTGG Cuts at: 2743 2743 Size: 5698 BstYI r'GATC_y Cuts at: 1972 2100 3028 3395 3780 3797 5249 5335 5347 Size: 128 928 367 385 17 1452 86 12 Cuts at: 5347 5693 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 928 385 367 346 128 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2439 2663 2892 513 Size: 552 1374 224 229 3319 Fragments arranged by size: 3319 1374 552 229 224 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2779 487 Size: 683 56 1553 3406 Fragments arranged by size: 3406 1553 683 56 BtsI GCAGTG_nn' Cuts at: 3214 4008 4028 3214 Size: 794 20 4884 Fragments arranged by size: 4884 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5698 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5663 DraI TTT'AAA Cuts at: 3002 3002 Size: 5698 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4705 809 Size: 507 3389 1802 Fragments arranged by size: 3389 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2130 2358 2580 Size: 54 1425 50 275 18 300 228 222 Cuts at: 2580 2895 3145 4034 5663 8 Size: 315 250 889 1629 43 Fragments arranged by size: 1629 1425 889 315 300 275 250 228 222 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2580 3145 1537 Size: 275 768 565 4090 Fragments arranged by size: 4090 768 565 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3628 1353 Size: 2275 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2783 4285 4659 4805 387 Size: 1064 138 1194 1502 374 146 1280 Fragments arranged by size: 1502 1280 1194 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2171 2252 2464 2699 2846 2864 3761 Size: 393 539 81 212 235 147 18 897 Cuts at: 3761 4358 5145 5413 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 897 787 597 539 393 268 235 212 147 81 18 EcoNI CCTnn'n_nnAGG Cuts at: 2681 2681 Size: 5698 EcoO109I rG'GnC_Cy Cuts at: 2674 2674 Size: 5698 EcoRI G'AATT_C Cuts at: 3007 3385 3007 Size: 378 5320 FseI GG_CCGG'CC Cuts at: 2586 2586 Size: 5698 FspI TGC'GCA Cuts at: 4204 4204 Size: 5698 HaeII r_GCGC'y Cuts at: 1678 2239 2487 2567 4845 1678 Size: 561 248 80 2278 2531 Fragments arranged by size: 2531 2278 561 248 80 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2732 2764 2873 2905 4339 Size: 32 135 32 1160 32 109 32 1434 Cuts at: 4339 4371 4413 4445 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1434 1160 135 109 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2416 2953 3042 3288 669 Size: 988 281 478 537 89 246 3079 Fragments arranged by size: 3079 988 537 478 281 246 89 HindIII A'AGCT_T Cuts at: 748 748 Size: 5698 HpaI GTT'AAC Cuts at: 2953 3288 2953 Size: 335 5363 KasI G'GCGC_C Cuts at: 2563 2563 Size: 5698 KpnI G_GTAC'C Cuts at: 3023 3023 Size: 5698 MfeI C'AATT_G Cuts at: 3297 3297 Size: 5698 MluI A'CGCG_T Cuts at: 5574 5574 Size: 5698 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4812 4996 1305 Size: 3507 184 2007 Fragments arranged by size: 3507 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3876 Fragments arranged by size: 3876 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2741 3656 4015 4174 518 Size: 648 1575 915 359 159 2042 Fragments arranged by size: 2042 1575 915 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2441 2466 2472 2553 2665 3114 3791 4939 Size: 835 25 6 81 112 449 677 1148 Cuts at: 4939 5184 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 835 677 449 245 112 81 25 6 NaeI GCC'GGC Cuts at: 2584 2584 Size: 5698 NarI GG'CG_CC Cuts at: 2564 2564 Size: 5698 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5146 NdeI CA'TA_TG Cuts at: 387 387 Size: 5698 NgoMIV G'CCGG_C Cuts at: 2582 2582 Size: 5698 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5698 NotI GC'GGCC_GC Cuts at: 3145 3145 Size: 5698 NspI r_CATG'y Cuts at: 1132 1229 2905 3056 4601 1132 Size: 97 1676 151 1545 2229 Fragments arranged by size: 2229 1676 1545 151 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5698 PciI A'CATG_T Cuts at: 4597 4597 Size: 5698 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5698 PmeI GTTT'AAAC Cuts at: 3002 3002 Size: 5698 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3767 3799 5280 5312 3767 Size: 32 1481 32 4153 Fragments arranged by size: 4153 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5698 PsiI TTA'TAA Cuts at: 3268 3268 Size: 5698 PstI C_TGCA'G Cuts at: 830 2673 3050 830 Size: 1843 377 3478 Fragments arranged by size: 3478 1843 377 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5698 SacII CC_GC'GG Cuts at: 2442 2666 2442 Size: 224 5474 SalI G'TCGA_C Cuts at: 1936 3040 1936 Size: 1104 4594 SbfI CC_TGCA'GG Cuts at: 3050 3050 Size: 5698 ScaI AGT'ACT Cuts at: 3946 5658 3946 Size: 1712 3986 SfcI C'TryA_G Cuts at: 826 1045 2086 2669 3046 4181 4862 5053 826 Size: 219 1041 583 377 1135 681 191 1471 Fragments arranged by size: 1471 1135 1041 681 583 377 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2588 2588 Size: 5698 SfoI GGC'GCC Cuts at: 2565 2565 Size: 5698 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5698 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5698 SmaI CCC'GGG Cuts at: 1872 3025 1872 Size: 1153 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2210 3802 4703 4965 820 Size: 197 926 267 1592 901 262 1553 Fragments arranged by size: 1592 1553 926 901 267 262 197 SnaBI TAC'GTA Cuts at: 493 3489 493 Size: 2996 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5698 SphI G_CATG'C Cuts at: 1132 3056 1132 Size: 1924 3774 SspI AAT'ATT Cuts at: 5 52 3622 5 Size: 47 3570 2081 Fragments arranged by size: 3570 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5698 StyI C'CwwG_G Cuts at: 513 1065 3137 513 Size: 552 2072 3074 Fragments arranged by size: 3074 2072 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2303 2949 3725 3742 3895 4080 4525 Size: 1255 723 646 776 17 153 185 445 Cuts at: 4525 325 Size: 1498 Fragments arranged by size: 1498 1255 776 723 646 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3944 5656 96 Size: 274 80 33 51 1443 1967 1712 138 Fragments arranged by size: 1967 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5658 5690 5658 Size: 32 5666 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5698 XbaI T'CTAG_A Cuts at: 3034 3034 Size: 5698 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2954 2954 Size: 5698 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5698 XmaI C'CCGG_G Cuts at: 1870 3023 1870 Size: 1153 4545 XmnI GAAnn'nnTTC Cuts at: 3827 3827 Size: 5698 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3506 276 Size: 53 83 186 936 1972 2468 Fragments arranged by size: 2468 1972 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI SrfI SwaI