Restriction map for
pFN21AB5528
(Circular) (Six-base) MAPSORT of: pFN21AB5528.seq Check: 2316 from: 1 to: 8974
pFN21AB5528 8974 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3497 3497
Size: 8974
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6784 278
Size: 53 83 186 936 5248 2468
Fragments arranged by size:
5248 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6317 1937
Size: 4380 4594
Acc65I G'GTAC_C
Cuts at: 6295 6295
Size: 8974
AclI AA'CG_TT
Cuts at: 1563 7101 7474 1563
Size: 5538 373 3063
Fragments arranged by size:
5538 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2342 3095 5372 5510 5824 7037 8421 8689
Size: 710 753 2277 138 314 1213 1384 268
Cuts at: 8689 1632
Size: 1917
Fragments arranged by size:
2277 1917 1384 1213 753 710 314 268
138
AflII C'TTAA_G
Cuts at: 820 1017 2513 820
Size: 197 1496 7281
Fragments arranged by size:
7281 1496 197
AflIII A'CryG_T
Cuts at: 1203 7873 8850 1203
Size: 6670 977 1327
Fragments arranged by size:
6670 1327 977
AgeI A'CCGG_T
Cuts at: 7805 7805
Size: 8974
AhdI GACnn_n'nnGTC
Cuts at: 7703 7703
Size: 8974
AleI CACnn'nnGTG
Cuts at: 1166 3783 1166
Size: 2617 6357
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2700 5714 6159 1184
Size: 1516 3014 445 3999
Fragments arranged by size:
3999 3014 1516 445
AlwNI CAG_nnn'CTG
Cuts at: 5536 6717 8289 5536
Size: 1181 1572 6221
Fragments arranged by size:
6221 1572 1181
ApaLI G'TGCA_C
Cuts at: 3512 4309 6179 7031 8187 3512
Size: 797 1870 852 1156 4299
Fragments arranged by size:
4299 1870 1156 852 797
ApoI r'AATT_y
Cuts at: 1493 2518 2851 4081 4804 5098 6106 6283 6509
Size: 1025 333 1230 723 294 1008 177 226
Cuts at: 6509 6661 8770 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1230 1025 1008 723 333 294
226 177 152
AseI AT'TA_AT
Cuts at: 160 1032 7528 160
Size: 872 6496 1606
Fragments arranged by size:
6496 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8974
AvaI C'yCGr_G
Cuts at: 1870 1943 6299 1870
Size: 73 4356 4545
Fragments arranged by size:
4545 4356 73
BamHI G'GATC_C
Cuts at: 2029 6304 6671 2029
Size: 4275 367 4332
Fragments arranged by size:
4332 4275 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 6295 7750 618
Size: 325 217 627 4508 1455 1842
Fragments arranged by size:
4508 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3387 5274 5651 6293 1391
Size: 1996 1887 377 642 4072
Fragments arranged by size:
4072 1996 1887 642 377
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2502 2637 4828 928
Size: 971 603 135 2191 5074
Fragments arranged by size:
5074 2191 971 603 135
BbvCI CC'TCA_GC
Cuts at: 4092 4092
Size: 8974
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4529 4563 5991 6025 7165 7199 1903
Size: 34 2592 34 1428 34 1140 34 3678
Fragments arranged by size:
3678 2592 1428 1140 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4015 6866 8076 4015
Size: 2851 1210 4913
Fragments arranged by size:
4913 2851 1210
BclI T'GATC_A
Cuts at: 1548 6259 1548
Size: 4711 4263
BfrBI ATG'CAT
Cuts at: 4024 4024
Size: 8974
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7585 136
Size: 107 122 71 631 605 5913 1525
Fragments arranged by size:
5913 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 8969 8969
Size: 8974
BlpI GC'TnA_GC
Cuts at: 2151 6391 2151
Size: 4240 4734
Bme1580I G_kGCm'C
Cuts at: 1790 3516 3580 4313 6183 7035 8191 1790
Size: 1726 64 733 1870 852 1156 2573
Fragments arranged by size:
2573 1870 1726 1156 852 733 64
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8974
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2990 3552 4131
Size: 416 53 83 399 24 1573 562 579
Cuts at: 4131 4771 4874 5033 6705 7663 442
Size: 640 103 159 1672 958 1753
Fragments arranged by size:
1753 1672 1573 958 640 579 562 416
399 159 103 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8974
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3797 3797
Size: 8974
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2061 3996 4017 4407 4593 7634 1239
Size: 822 1935 21 390 186 3041 2579
Fragments arranged by size:
3041 2579 1935 822 390 186 21
Bpu10I CC'TnA_GC
Cuts at: 4092 4092
Size: 8974
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4318 4585 4687 7099 7964 8262 4318
Size: 267 102 2412 865 298 5030
Fragments arranged by size:
5030 2412 865 298 267 102
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2555 4912 5479 7637 882
Size: 617 1056 2357 567 2158 2219
Fragments arranged by size:
2357 2219 2158 1056 617 567
BsaAI yAC'GTr
Cuts at: 493 1204 6765 493
Size: 711 5561 2702
Fragments arranged by size:
5561 2702 711
BsaBI GATnn'nnATC
Cuts at: 8968 8968
Size: 8974
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6781 7163 7798 275
Size: 53 83 186 936 5248 382 635 1451
Fragments arranged by size:
5248 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4050 7407 7805 8079 8226 8637 4050
Size: 3357 398 274 147 411 4387
Fragments arranged by size:
4387 3357 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3403 3433 4729 4759 1178
Size: 30 165 30 2000 30 1296 30 5393
Fragments arranged by size:
5393 2000 1296 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3444 3973 3976 4014 4845 5904 1186
Size: 2258 529 3 38 831 1059 4256
Fragments arranged by size:
4256 2258 1059 831 529 38 3
BseYI C'CCAG_C
Cuts at: 5350 5870 6132 8177 8673 5350
Size: 520 262 2045 496 5651
Fragments arranged by size:
5651 2045 520 496 262
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3021 4559 4845 5159 1163
Size: 573 1285 1538 286 314 4978
Fragments arranged by size:
4978 1538 1285 573 314 286
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6424 7185 8213 1540
Size: 275 186 4423 761 1028 2301
Fragments arranged by size:
4423 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3516 4313 5651 6183 7035 7120 8191 3516
Size: 797 1338 532 852 85 1071 4299
Fragments arranged by size:
4299 1338 1071 852 797 532 85
BsmI GAATG_Cn'
Cuts at: 5235 5466 5523 6485 6578 5235
Size: 231 57 962 93 7631
Fragments arranged by size:
7631 962 231 93 57
BsmBI CGTCTCn'nnnn_
Cuts at: 2107 2388 2107
Size: 281 8693
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3387 3516 3580 4313 5274 5651 6183
Size: 399 1597 129 64 733 961 377 532
Cuts at: 6183 6293 7035 7120 8191 1391
Size: 110 742 85 1071 2174
Fragments arranged by size:
2174 1597 1071 961 742 733 532 399
377 129 110 85 64
BspEI T'CCGG_A
Cuts at: 8637 8637
Size: 8974
BspHI T'CATG_A
Cuts at: 3444 5481 6863 8593 3444
Size: 2037 1382 1730 3825
Fragments arranged by size:
3825 2037 1730 1382
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2102 2445 3497 4233 4867 6329 844
Size: 1258 343 1052 736 634 1462 3489
Fragments arranged by size:
3489 1462 1258 1052 736 634 343
BsrBI CCG'CTC
Cuts at: 1394 1823 6861 1394
Size: 429 5038 3507
Fragments arranged by size:
5038 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4883 6009 7469 7643 65
Size: 1183 3635 1126 1460 174 1396
Fragments arranged by size:
3635 1460 1396 1183 1126 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4784 7618 7805 1700
Size: 110 2974 2834 187 2869
Fragments arranged by size:
2974 2869 2834 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8974
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8974
BssSI C'ACGA_G
Cuts at: 2700 5469 7034 8046 2700
Size: 2769 1565 1012 3628
Fragments arranged by size:
3628 2769 1565 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4066 4945 5536 794
Size: 3272 879 591 4232
Fragments arranged by size:
4232 3272 879 591
BstBI TT'CG_AA
Cuts at: 7778 7778
Size: 8974
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8974
BstXI CCAn_nnnn'nTGG
Cuts at: 4981 5209 4981
Size: 228 8746
BstYI r'GATC_y
Cuts at: 1972 2029 2861 4382 5965 6304 6671 7056 7073
Size: 57 832 1521 1583 339 367 385 17
Cuts at: 7073 8525 8611 8623 8969 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1583 1521 1452 832 385 367 346
339 86 57 17 12
Bsu36I CC'TnA_GG
Cuts at: 2341 4753 2341
Size: 2412 6562
BtgI C'CryG_G
Cuts at: 513 1065 3522 4815 4974 5052 513
Size: 552 2457 1293 159 78 4435
Fragments arranged by size:
4435 2457 1293 552 159 78
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8235
Fragments arranged by size:
8235 683 56
BtsI GCAGTG_nn'
Cuts at: 2104 2684 3814 4575 6171 6202 6490 7284 7304
Size: 580 1130 761 1596 31 288 794 20
Cuts at: 7304 2104
Size: 3774
Fragments arranged by size:
3774 1596 1130 794 761 580 288 31
20
ClaI AT'CG_AT
Cuts at: 1554 3004 1554
Size: 1450 7524
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8939
DraI TTT'AAA
Cuts at: 2220 6278 2220
Size: 4058 4916
DraIII CAC_nnn'GTG
Cuts at: 4868 4868
Size: 8974
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2241 7981 809
Size: 507 925 5740 1802
Fragments arranged by size:
5740 1802 925 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2037 2980 4999
Size: 54 1425 50 275 18 207 943 2019
Cuts at: 4999 6095 6111 6421 7310 8939 8
Size: 1096 16 310 889 1629 43
Fragments arranged by size:
2019 1629 1425 1096 943 889 310 275
207 54 50 43 18 16
EagI C'GGCC_G
Cuts at: 1537 1812 6421 1537
Size: 275 4609 4090
Fragments arranged by size:
4609 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3062 4371 4896 5094 5187 6904 1353
Size: 1709 1309 525 198 93 1717 3423
Fragments arranged by size:
3423 1717 1709 1309 525 198 93
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7561 7935 8081 387
Size: 1064 138 5972 374 146 1280
Fragments arranged by size:
5972 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 5649 5649
Size: 8974
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2061 2342 3095 3996 4017 4407 4593
Size: 393 429 281 753 901 21 390 186
Cuts at: 4593 5372 5510 5824 7037 7634 8421 8689 1239
Size: 779 138 314 1213 597 787 268 1524
Fragments arranged by size:
1524 1213 901 787 779 753 597 429
393 390 314 281 268 186 138 21
EcoNI CCTnn'n_nnAGG
Cuts at: 2869 3339 3504 4752 5454 2869
Size: 470 165 1248 702 6389
Fragments arranged by size:
6389 1248 702 470 165
EcoO109I rG'GnC_Cy
Cuts at: 2455 3249 3638 3836 5360 5894 6023 6254 2455
Size: 794 389 198 1524 534 129 231 5175
Fragments arranged by size:
5175 1524 794 534 389 231 198 129
EcoRI G'AATT_C
Cuts at: 4081 4804 5098 6283 6661 4081
Size: 723 294 1185 378 6394
Fragments arranged by size:
6394 1185 723 378 294
EcoRV GAT'ATC
Cuts at: 2160 2160
Size: 8974
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2990 3855 2990
Size: 865 8109
FspI TGC'GCA
Cuts at: 7480 7480
Size: 8974
HaeII r_GCGC'y
Cuts at: 1678 8121 1678
Size: 6443 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2144 2176 2321 2353 2717
Size: 32 135 32 572 32 145 32 364
Cuts at: 2717 2749 2921 2953 3403 3435 3439 3471 4241
Size: 32 172 32 450 32 4 32 770
Cuts at: 4241 4273 4669 4701 4723 4729 4755 4761 5463
Size: 32 396 32 22 6 26 6 702
Cuts at: 5463 5495 7615 7647 7689 7721 1373
Size: 32 2120 32 42 32 2626
Fragments arranged by size:
2626 2120 770 702 572 450 396 364
172 145 135 42 32 32 32 32
32 32 32 32 32 32 32 32
32 26 22 6 6 4
HincII GTy'rAC
Cuts at: 669 1657 1938 2464 6318 6564 669
Size: 988 281 526 3854 246 3079
Fragments arranged by size:
3854 3079 988 526 281 246
HindIII A'AGCT_T
Cuts at: 748 2967 748
Size: 2219 6755
HpaI GTT'AAC
Cuts at: 2464 6564 2464
Size: 4100 4874
KpnI G_GTAC'C
Cuts at: 6299 6299
Size: 8974
MfeI C'AATT_G
Cuts at: 6573 6573
Size: 8974
MluI A'CGCG_T
Cuts at: 8850 8850
Size: 8974
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3133 5154 5740 8088 8272 1305
Size: 1828 2021 586 2348 184 2007
Fragments arranged by size:
2348 2021 2007 1828 586 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2039 2982 5001 6097 6113
Size: 54 1425 343 207 943 2019 1096 16
Cuts at: 6113 10
Size: 2871
Fragments arranged by size:
2871 2019 1425 1096 943 343 207 54
16
MslI CAynn'nnrTG
Cuts at: 518 1166 3209 3783 4979 6186 6233 6248 6932
Size: 648 2043 574 1196 1207 47 15 684
Cuts at: 6932 7291 7450 518
Size: 359 159 2042
Fragments arranged by size:
2043 2042 1207 1196 684 648 574 359
159 47 15
MspA1I CmG'CkG
Cuts at: 1606 4091 5173 6390 7067 8215 8460 1606
Size: 2485 1082 1217 677 1148 245 2120
Fragments arranged by size:
2485 2120 1217 1148 1082 677 245
NaeI GCC'GGC
Cuts at: 4786 4786
Size: 8974
NcoI C'CATG_G
Cuts at: 513 1065 3522 4815 4974 5052 513
Size: 552 2457 1293 159 78 4435
Fragments arranged by size:
4435 2457 1293 552 159 78
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8974
NgoMIV G'CCGG_C
Cuts at: 4784 4784
Size: 8974
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8974
NotI GC'GGCC_GC
Cuts at: 6421 6421
Size: 8974
NsiI A_TGCA'T
Cuts at: 4026 4026
Size: 8974
NspI r_CATG'y
Cuts at: 1132 1229 3349 5047 6247 6332 7877 1132
Size: 97 2120 1698 1200 85 1545 2229
Fragments arranged by size:
2229 2120 1698 1545 1200 97 85
PasI CC'CwG_GG
Cuts at: 1348 3335 5653 1348
Size: 1987 2318 4669
Fragments arranged by size:
4669 2318 1987
PciI A'CATG_T
Cuts at: 7873 7873
Size: 8974
PflMI CCAn_nnn'nTGG
Cuts at: 1108 5945 5975 1108
Size: 4837 30 4107
Fragments arranged by size:
4837 4107 30
PfoI T'CCnGG_A
Cuts at: 2077 2549 2077
Size: 472 8502
PmeI GTTT'AAAC
Cuts at: 6278 6278
Size: 8974
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4495 4527 7043 7075 8556 8588 4495
Size: 32 2516 32 1481 32 4881
Fragments arranged by size:
4881 2516 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2455 3836 5894 6023 6254 2455
Size: 1381 2058 129 231 5175
Fragments arranged by size:
5175 2058 1381 231 129
PshAI GACnn'nnGTC
Cuts at: 1613 3900 1613
Size: 2287 6687
PsiI TTA'TAA
Cuts at: 4839 6544 4839
Size: 1705 7269
PstI C_TGCA'G
Cuts at: 830 2099 3873 4074 4114 5541 6166 6326 830
Size: 1269 1774 201 40 1427 625 160 3478
Fragments arranged by size:
3478 1774 1427 1269 625 201 160 40
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8974
PvuII CAG'CTG
Cuts at: 4091 5173 4091
Size: 1082 7892
SacI G_AGCT'C
Cuts at: 5651 5651
Size: 8974
SalI G'TCGA_C
Cuts at: 1936 6316 1936
Size: 4380 4594
SanDI GG'GwC_CC
Cuts at: 6023 6023
Size: 8974
SbfI CC_TGCA'GG
Cuts at: 6326 6326
Size: 8974
ScaI AGT'ACT
Cuts at: 7222 8934 7222
Size: 1712 7262
SexAI A'CCwGG_T
Cuts at: 2457 4909 2457
Size: 2452 6522
SfcI C'TryA_G
Cuts at: 826 1045 2095 2310 2532 2766 3027 3869 4070
Size: 219 1050 215 222 234 261 842 201
Cuts at: 4070 4110 4268 5537 6162 6322 7457 8138 8329
Size: 40 158 1269 625 160 1135 681 191
Cuts at: 8329 826
Size: 1471
Fragments arranged by size:
1471 1269 1135 1050 842 681 625 261
234 222 219 215 201 191 160 158
40
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8974
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8974
SmaI CCC'GGG
Cuts at: 1872 6301 1872
Size: 4429 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2513 4333 4600 4702 7078 7979
Size: 197 926 570 1820 267 102 2376 901
Cuts at: 7979 8241 820
Size: 262 1553
Fragments arranged by size:
2376 1820 1553 926 901 570 267 262
197 102
SnaBI TAC'GTA
Cuts at: 493 6765 493
Size: 6272 2702
SpeI A'CTAG_T
Cuts at: 152 4427 152
Size: 4275 4699
SphI G_CATG'C
Cuts at: 1132 3349 6247 6332 1132
Size: 2217 2898 85 3774
Fragments arranged by size:
3774 2898 2217 85
SspI AAT'ATT
Cuts at: 5 52 3670 6898 5
Size: 47 3618 3228 2081
Fragments arranged by size:
3618 3228 2081 47
StuI AGG'CCT
Cuts at: 1517 2875 4960 5086 5208 5626 5837 1517
Size: 1358 2085 126 122 418 211 4654
Fragments arranged by size:
4654 2085 1358 418 211 126 122
StyI C'CwwG_G
Cuts at: 513 1065 3223 3522 4815 4974 5052 5290 5455
Size: 552 2158 299 1293 159 78 238 165
Cuts at: 5455 6413 513
Size: 958 3074
Fragments arranged by size:
3074 2158 1293 958 552 299 238 165
159 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4649 4700 4856 4884 5481 7001 7018
Size: 1255 3069 51 156 28 597 1520 17
Cuts at: 7018 7171 7356 7801 325
Size: 153 185 445 1498
Fragments arranged by size:
3069 1520 1498 1255 597 445 185 156
153 51 28 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 7220 8932 96
Size: 274 80 33 51 1443 5243 1712 138
Fragments arranged by size:
5243 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3015 3047 3542 3574 8934 8966 3015
Size: 32 495 32 5360 32 3023
Fragments arranged by size:
5360 3023 495 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2085 1316
Size: 769 8205
XbaI T'CTAG_A
Cuts at: 4402 6310 4402
Size: 1908 7066
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3712 4861 3712
Size: 1149 7825
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8974
XmaI C'CCGG_G
Cuts at: 1870 6299 1870
Size: 4429 4545
XmnI GAAnn'nnTTC
Cuts at: 4507 7103 4507
Size: 2596 6378
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6782 276
Size: 53 83 186 936 5248 2468
Fragments arranged by size:
5248 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BsiWI
BstZ17I FseI FspAI KasI NarI NruI PacI PmlI
PspOMI PspXI PsrI RsrII SacII SapI SfiI SfoI
SrfI SwaI