(Circular) (Six-base) MAPSORT of: pFN21AB5456.seq Check: 2745 from: 1 to: 5962 pFN21AB5456 5962 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2133 2587 2686 2133 Size: 454 99 5409 Fragments arranged by size: 5409 454 99 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2447 3772 278 Size: 53 83 186 936 911 1325 2468 Fragments arranged by size: 2468 1325 936 911 186 83 53 AccI GT'mk_AC Cuts at: 1937 3305 1937 Size: 1368 4594 Acc65I G'GTAC_C Cuts at: 3283 3283 Size: 5962 AclI AA'CG_TT Cuts at: 1563 4089 4462 1563 Size: 2526 373 3063 Fragments arranged by size: 3063 2526 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2115 2366 2408 2876 2927 3053 4025 5409 Size: 483 251 42 468 51 126 972 1384 Cuts at: 5409 5677 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 972 483 468 268 251 126 51 42 AfeI AGC'GCT Cuts at: 2162 2228 2162 Size: 66 5896 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5765 AflIII A'CryG_T Cuts at: 1203 3241 4861 5838 1203 Size: 2038 1620 977 1327 Fragments arranged by size: 2038 1620 1327 977 AgeI A'CCGG_T Cuts at: 4793 4793 Size: 5962 AhdI GACnn_n'nnGTC Cuts at: 4691 4691 Size: 5962 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5962 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5962 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2261 2293 2261 Size: 32 5930 AlwNI CAG_nnn'CTG Cuts at: 2218 2368 2512 3705 5277 2218 Size: 150 144 1193 1572 2903 Fragments arranged by size: 2903 1572 1193 150 144 ApaI G_GGCC'C Cuts at: 2527 2527 Size: 5962 ApaLI G'TGCA_C Cuts at: 4019 5175 4019 Size: 1156 4806 ApoI r'AATT_y Cuts at: 1493 3271 3497 3649 5758 1493 Size: 1778 226 152 2109 1697 Fragments arranged by size: 2109 1778 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4516 160 Size: 872 3484 1606 Fragments arranged by size: 3484 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5962 AvaI C'yCGr_G Cuts at: 1870 1943 2453 2526 2958 3170 3287 1870 Size: 73 510 73 432 212 117 4545 Fragments arranged by size: 4545 510 432 212 117 73 73 BamHI G'GATC_C Cuts at: 3292 3659 3292 Size: 367 5595 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2013 3283 4738 618 Size: 325 217 627 226 1270 1455 1842 Fragments arranged by size: 1842 1455 1270 627 325 226 217 BanII G_rGCy'C Cuts at: 1391 2187 2527 2988 3167 3281 1391 Size: 796 340 461 179 114 4072 Fragments arranged by size: 4072 796 461 340 179 114 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4991 BbvCI CC'TCA_GC Cuts at: 2412 2412 Size: 5962 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4153 4187 1903 Size: 34 2216 34 3678 Fragments arranged by size: 3678 2216 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3854 5064 3854 Size: 1210 4752 BclI T'GATC_A Cuts at: 1548 1548 Size: 5962 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2531 2819 2828 Size: 107 122 71 631 605 859 288 9 Cuts at: 2828 4573 136 Size: 1745 1525 Fragments arranged by size: 1745 1525 859 631 605 288 122 107 71 9 BglII A'GATC_T Cuts at: 2788 5957 2788 Size: 3169 2793 BlpI GC'TnA_GC Cuts at: 3379 3379 Size: 5962 Bme1580I G_kGCm'C Cuts at: 1790 2527 4023 5179 1790 Size: 737 1496 1156 2573 Fragments arranged by size: 2573 1496 1156 737 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5962 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2046 2093 2683 Size: 416 53 83 399 24 629 47 590 Cuts at: 2683 3693 4651 442 Size: 1010 958 1753 Fragments arranged by size: 1753 1010 958 629 590 416 399 83 53 47 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5962 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2141 2189 2351 2534 2576 2789 3023 4622 Size: 902 48 162 183 42 213 234 1599 Cuts at: 4622 1239 Size: 2579 Fragments arranged by size: 2579 1599 902 234 213 183 162 48 42 Bpu10I CC'TnA_GC Cuts at: 2412 2412 Size: 5962 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4087 4952 5250 4087 Size: 865 298 4799 Fragments arranged by size: 4799 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2144 2885 3097 4625 882 Size: 617 645 741 212 1528 2219 Fragments arranged by size: 2219 1528 741 645 617 212 BsaAI yAC'GTr Cuts at: 493 1204 3753 493 Size: 711 2549 2702 Fragments arranged by size: 2702 2549 711 BsaBI GATnn'nnATC Cuts at: 5956 5956 Size: 5962 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2444 3769 4151 4786 Size: 53 83 186 936 911 1325 382 635 Cuts at: 4786 275 Size: 1451 Fragments arranged by size: 1451 1325 936 911 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2899 4395 4793 5067 5214 5625 2899 Size: 1496 398 274 147 411 3236 Fragments arranged by size: 3236 1496 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2261 2291 2787 2817 1178 Size: 30 165 30 858 30 496 30 4323 Fragments arranged by size: 4323 858 496 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2088 2186 2249 2282 2684 2831 2876 3121 Size: 902 98 63 33 402 147 45 245 Cuts at: 3121 3172 3175 3188 1186 Size: 51 3 13 3960 Fragments arranged by size: 3960 902 402 245 147 98 63 51 45 33 13 3 BseYI C'CCAG_C Cuts at: 3149 5165 5661 3149 Size: 2016 496 3450 Fragments arranged by size: 3450 2016 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2066 2105 2615 1163 Size: 573 330 39 510 4510 Fragments arranged by size: 4510 573 510 330 39 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3412 4173 5201 1540 Size: 275 186 1411 761 1028 2301 Fragments arranged by size: 2301 1411 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2187 2385 2988 4023 4108 5179 2187 Size: 198 603 1035 85 1071 2970 Fragments arranged by size: 2970 1071 1035 603 198 85 BsmI GAATG_Cn' Cuts at: 2507 2781 3473 3566 2507 Size: 274 692 93 4903 Fragments arranged by size: 4903 692 274 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2187 2385 2527 2988 3167 3281 4023 Size: 399 397 198 142 461 179 114 742 Cuts at: 4023 4108 5179 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 461 399 397 198 179 142 114 85 BspEI T'CCGG_A Cuts at: 5625 5625 Size: 5962 BspHI T'CATG_A Cuts at: 3851 5581 3851 Size: 1730 4232 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2133 2587 2686 3317 844 Size: 1289 454 99 631 3489 Fragments arranged by size: 3489 1289 631 454 99 BsrBI CCG'CTC Cuts at: 1394 1823 2764 3849 1394 Size: 429 941 1085 3507 Fragments arranged by size: 3507 1085 941 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4457 4631 65 Size: 1183 3209 174 1396 Fragments arranged by size: 3209 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4606 4793 1700 Size: 110 2796 187 2869 Fragments arranged by size: 2869 2796 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5962 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5962 BssSI C'ACGA_G Cuts at: 4022 5034 4022 Size: 1012 4950 BstAPI GCAn_nnn'nTGC Cuts at: 794 2368 2593 794 Size: 1574 225 4163 Fragments arranged by size: 4163 1574 225 BstBI TT'CG_AA Cuts at: 4766 4766 Size: 5962 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5962 BstXI CCAn_nnnn'nTGG Cuts at: 2311 2311 Size: 5962 BstYI r'GATC_y Cuts at: 1972 2788 3292 3659 4044 4061 5513 5599 5611 Size: 816 504 367 385 17 1452 86 12 Cuts at: 5611 5957 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 816 504 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5410 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5223 Fragments arranged by size: 5223 683 56 BtsI GCAGTG_nn' Cuts at: 2764 3478 4272 4292 2764 Size: 714 794 20 4434 Fragments arranged by size: 4434 794 714 20 ClaI AT'CG_AT Cuts at: 1554 2847 1554 Size: 1293 4669 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5927 DraI TTT'AAA Cuts at: 3266 3266 Size: 5962 DraIII CAC_nnn'GTG Cuts at: 2593 2593 Size: 5962 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4969 809 Size: 507 3653 1802 Fragments arranged by size: 3653 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2532 2829 2970 Size: 54 1425 50 275 18 702 297 141 Cuts at: 2970 3054 3409 4298 5927 8 Size: 84 355 889 1629 43 Fragments arranged by size: 1629 1425 889 702 355 297 275 141 84 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3409 1537 Size: 275 1597 4090 Fragments arranged by size: 4090 1597 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2018 2085 2342 2384 2669 2720 3892 1353 Size: 665 67 257 42 285 51 1172 3423 Fragments arranged by size: 3423 1172 665 285 257 67 51 42 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2933 2948 4549 4923 5069 387 Size: 1064 138 1344 15 1601 374 146 1280 Fragments arranged by size: 1601 1344 1280 1064 374 146 138 15 EcoICRI GAG'CTC Cuts at: 2185 2986 2185 Size: 801 5161 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2115 2141 2189 2351 2366 2408 2534 Size: 393 483 26 48 162 15 42 126 Cuts at: 2534 2576 2789 2876 2927 3023 3053 4025 4622 Size: 42 213 87 51 96 30 972 597 Cuts at: 4622 5409 5677 1239 Size: 787 268 1524 Fragments arranged by size: 1524 972 787 597 483 393 268 213 162 126 96 87 51 48 42 42 30 26 15 EcoO109I rG'GnC_Cy Cuts at: 2821 3100 3214 2821 Size: 279 114 5569 Fragments arranged by size: 5569 279 114 EcoRI G'AATT_C Cuts at: 3271 3649 3271 Size: 378 5584 EcoRV GAT'ATC Cuts at: 2923 2923 Size: 5962 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2953 2953 Size: 5962 FspI TGC'GCA Cuts at: 2612 4468 2612 Size: 1856 4106 HaeII r_GCGC'y Cuts at: 1678 2145 2164 2230 2576 2656 5109 1678 Size: 467 19 66 346 80 2453 2531 Fragments arranged by size: 2531 2453 467 346 80 66 19 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2181 2213 2408 2440 2461 Size: 32 135 32 609 32 195 32 21 Cuts at: 2461 2493 4603 4635 4677 4709 1373 Size: 32 2110 32 42 32 2626 Fragments arranged by size: 2626 2110 609 195 135 42 32 32 32 32 32 32 32 21 HincII GTy'rAC Cuts at: 669 1657 1938 3306 3552 669 Size: 988 281 1368 246 3079 Fragments arranged by size: 3079 1368 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5962 HpaI GTT'AAC Cuts at: 3552 3552 Size: 5962 KpnI G_GTAC'C Cuts at: 3287 3287 Size: 5962 MfeI C'AATT_G Cuts at: 3561 3561 Size: 5962 MluI A'CGCG_T Cuts at: 5838 5838 Size: 5962 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5076 5260 1305 Size: 3771 184 2007 Fragments arranged by size: 3771 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2831 10 Size: 54 1425 343 999 3141 Fragments arranged by size: 3141 1425 999 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3920 4279 4438 518 Size: 648 2754 359 159 2042 Fragments arranged by size: 2754 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2554 2608 2782 3001 3010 3042 3378 4055 Size: 948 54 174 219 9 32 336 677 Cuts at: 4055 5203 5448 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 948 677 336 245 219 174 54 32 9 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5410 NdeI CA'TA_TG Cuts at: 387 387 Size: 5962 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5962 NotI GC'GGCC_GC Cuts at: 3409 3409 Size: 5962 NspI r_CATG'y Cuts at: 1132 1229 2617 3086 3245 3320 4865 1132 Size: 97 1388 469 159 75 1545 2229 Fragments arranged by size: 2229 1545 1388 469 159 97 75 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5962 PciI A'CATG_T Cuts at: 3241 4861 3241 Size: 1620 4342 PflMI CCAn_nnn'nTGG Cuts at: 1108 2512 2629 1108 Size: 1404 117 4441 Fragments arranged by size: 4441 1404 117 PfoI T'CCnGG_A Cuts at: 2295 2295 Size: 5962 PmeI GTTT'AAAC Cuts at: 3266 3266 Size: 5962 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2261 2293 4031 4063 5544 5576 2261 Size: 32 1738 32 1481 32 2647 Fragments arranged by size: 2647 1738 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5962 PsiI TTA'TAA Cuts at: 3532 3532 Size: 5962 PspOMI G'GGCC_C Cuts at: 2523 2523 Size: 5962 PstI C_TGCA'G Cuts at: 830 2466 2580 2994 3015 3237 3314 830 Size: 1636 114 414 21 222 77 3478 Fragments arranged by size: 3478 1636 414 222 114 77 21 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5962 PvuII CAG'CTG Cuts at: 2554 2608 2782 3001 3010 3042 2554 Size: 54 174 219 9 32 5474 Fragments arranged by size: 5474 219 174 54 32 9 SacI G_AGCT'C Cuts at: 2187 2988 2187 Size: 801 5161 SalI G'TCGA_C Cuts at: 1936 3304 1936 Size: 1368 4594 SapI GCTCTTCn'nnn_ Cuts at: 2018 2720 2018 Size: 702 5260 SbfI CC_TGCA'GG Cuts at: 2994 3314 2994 Size: 320 5642 ScaI AGT'ACT Cuts at: 4210 5922 4210 Size: 1712 4250 SfcI C'TryA_G Cuts at: 826 1045 2462 2576 2990 3011 3233 3310 4445 Size: 219 1417 114 414 21 222 77 1135 Cuts at: 4445 5126 5317 826 Size: 681 191 1471 Fragments arranged by size: 1471 1417 1135 681 414 222 219 191 114 77 21 SfiI GGCCn_nnn'nGGCC Cuts at: 2531 2819 2828 2531 Size: 288 9 5665 Fragments arranged by size: 5665 288 9 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5962 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5962 SmaI CCC'GGG Cuts at: 1872 2528 2960 3289 1872 Size: 656 432 329 4545 Fragments arranged by size: 4545 656 432 329 SmlI C'TyrA_G Cuts at: 820 1017 1943 4066 4967 5229 820 Size: 197 926 2123 901 262 1553 Fragments arranged by size: 2123 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3753 493 Size: 3260 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5962 SphI G_CATG'C Cuts at: 1132 2617 3320 1132 Size: 1485 703 3774 Fragments arranged by size: 3774 1485 703 SrfI GCCC'GGGC Cuts at: 2528 2528 Size: 5962 SspI AAT'ATT Cuts at: 5 52 3886 5 Size: 47 3834 2081 Fragments arranged by size: 3834 2081 47 StuI AGG'CCT Cuts at: 1517 2423 1517 Size: 906 5056 StyI C'CwwG_G Cuts at: 513 1065 3401 513 Size: 552 2336 3074 Fragments arranged by size: 3074 2336 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3989 4006 4159 4344 4789 325 Size: 1255 2409 17 153 185 445 1498 Fragments arranged by size: 2409 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3021 4208 5920 Size: 274 80 33 51 1443 1044 1187 1712 Cuts at: 5920 96 Size: 138 Fragments arranged by size: 1712 1443 1187 1044 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5922 5954 5922 Size: 32 5930 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5962 XbaI T'CTAG_A Cuts at: 3298 3298 Size: 5962 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2311 2311 Size: 5962 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5962 XmaI C'CCGG_G Cuts at: 1870 2526 2958 3287 1870 Size: 656 432 329 4545 Fragments arranged by size: 4545 656 432 329 XmnI GAAnn'nnTTC Cuts at: 2276 4091 2276 Size: 1815 4147 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2445 3770 276 Size: 53 83 186 936 911 1325 2468 Fragments arranged by size: 2468 1325 936 911 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SexAI SfoI SwaI