Restriction map for
pFN21AB5456
(Linear) (Six-base) MAPSORT of: KIBB5456.seq Check: 1645 from: 1 to: 1257
KIBB5456.orf
With 182 enzymes: SgfI *
February 28, 2007 11:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 128 582 681 1257
Size: 128 454 99 576
Fragments arranged by size:
576 454 128 99
AatII G_ACGT'C
Cuts at: 0 442 1257
Size: 442 815
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 110 361 403 871 922 1048 1257
Size: 110 251 42 468 51 126 209
Fragments arranged by size:
468 251 209 126 110 51 42
AfeI AGC'GCT
Cuts at: 0 157 223 1257
Size: 157 66 1034
Fragments arranged by size:
1034 157 66
AflIII A'CryG_T
Cuts at: 0 1236 1257
Size: 1236 21
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 256 288 1257
Size: 256 32 969
Fragments arranged by size:
969 256 32
AlwNI CAG_nnn'CTG
Cuts at: 0 213 363 507 1257
Size: 213 150 144 750
Fragments arranged by size:
750 213 150 144
ApaI G_GGCC'C
Cuts at: 0 522 1257
Size: 522 735
AvaI C'yCGr_G
Cuts at: 0 448 521 953 1165 1257
Size: 448 73 432 212 92
Fragments arranged by size:
448 432 212 92 73
BanI G'GyrC_C
Cuts at: 0 8 1257
Size: 8 1249
BanII G_rGCy'C
Cuts at: 0 182 522 983 1162 1257
Size: 182 340 461 179 95
Fragments arranged by size:
461 340 182 179 95
BbvCI CC'TCA_GC
Cuts at: 0 407 1257
Size: 407 850
BglI GCCn_nnn'nGGC
Cuts at: 0 526 814 823 1257
Size: 526 288 9 434
Fragments arranged by size:
526 434 288 9
BglII A'GATC_T
Cuts at: 0 783 1257
Size: 783 474
Bme1580I G_kGCm'C
Cuts at: 0 522 1257
Size: 522 735
BmrI ACTGGGnnnn_n'
Cuts at: 0 41 88 678 1257
Size: 41 47 590 579
Fragments arranged by size:
590 579 47 41
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 184 346 529 571 784 1018 1257
Size: 136 48 162 183 42 213 234 239
Fragments arranged by size:
239 234 213 183 162 136 48 42
Bpu10I CC'TnA_GC
Cuts at: 0 407 1257
Size: 407 850
BsaI GGTCTCn'nnnn_
Cuts at: 0 139 880 1092 1257
Size: 139 741 212 165
Fragments arranged by size:
741 212 165 139
BsaHI Gr'CG_yC
Cuts at: 0 439 1257
Size: 439 818
BsaWI w'CCGG_w
Cuts at: 0 894 1257
Size: 894 363
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 256 286 782 812 1257
Size: 256 30 496 30 445
Fragments arranged by size:
496 445 256 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 83 181 244 277 679 826 871 1116
Size: 83 98 63 33 402 147 45 245
Cuts at: 1116 1167 1170 1183 1257
Size: 51 3 13 74
Fragments arranged by size:
402 245 147 98 83 74 63 51
45 33 13 3
BseYI C'CCAG_C
Cuts at: 0 1144 1257
Size: 1144 113
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 100 610 1257
Size: 61 39 510 647
Fragments arranged by size:
647 510 61 39
BsiHKAI G_wGCw'C
Cuts at: 0 182 380 983 1257
Size: 182 198 603 274
Fragments arranged by size:
603 274 198 182
BsmI GAATG_Cn'
Cuts at: 0 502 776 1257
Size: 502 274 481
Fragments arranged by size:
502 481 274
Bsp1286I G_dGCh'C
Cuts at: 0 182 380 522 983 1162 1257
Size: 182 198 142 461 179 95
Fragments arranged by size:
461 198 182 179 142 95
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 128 582 681 1257
Size: 128 454 99 576
Fragments arranged by size:
576 454 128 99
BsrBI CCG'CTC
Cuts at: 0 759 1257
Size: 759 498
BstAPI GCAn_nnn'nTGC
Cuts at: 0 363 588 1257
Size: 363 225 669
Fragments arranged by size:
669 363 225
BstXI CCAn_nnnn'nTGG
Cuts at: 0 306 1257
Size: 306 951
BstYI r'GATC_y
Cuts at: 0 783 1257
Size: 783 474
BtsI GCAGTG_nn'
Cuts at: 0 759 1257
Size: 759 498
ClaI AT'CG_AT
Cuts at: 0 842 1257
Size: 842 415
DraIII CAC_nnn'GTG
Cuts at: 0 588 1257
Size: 588 669
EaeI y'GGCC_r
Cuts at: 0 527 824 965 1049 1257
Size: 527 297 141 84 208
Fragments arranged by size:
527 297 208 141 84
EarI CTCTTCn'nnn_
Cuts at: 0 13 80 337 379 664 715 1257
Size: 13 67 257 42 285 51 542
Fragments arranged by size:
542 285 257 67 51 42 13
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 928 943 1257
Size: 928 15 314
Fragments arranged by size:
928 314 15
EcoICRI GAG'CTC
Cuts at: 0 180 981 1257
Size: 180 801 276
Fragments arranged by size:
801 276 180
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 110 136 184 346 361 403 529 571
Size: 110 26 48 162 15 42 126 42
Cuts at: 571 784 871 922 1018 1048 1257
Size: 213 87 51 96 30 209
Fragments arranged by size:
213 209 162 126 110 96 87 51
48 42 42 30 26 15
EcoO109I rG'GnC_Cy
Cuts at: 0 816 1095 1209 1257
Size: 816 279 114 48
Fragments arranged by size:
816 279 114 48
EcoRV GAT'ATC
Cuts at: 0 918 1257
Size: 918 339
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 948 1257
Size: 948 309
FspI TGC'GCA
Cuts at: 0 607 1257
Size: 607 650
HaeII r_GCGC'y
Cuts at: 0 140 159 225 571 651 1257
Size: 140 19 66 346 80 606
Fragments arranged by size:
606 346 140 80 66 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 176 208 403 435 456 488 1257
Size: 176 32 195 32 21 32 769
Fragments arranged by size:
769 195 176 32 32 32 21
MscI TGG'CCA
Cuts at: 0 826 1257
Size: 826 431
MspA1I CmG'CkG
Cuts at: 0 549 603 777 996 1005 1037 1257
Size: 549 54 174 219 9 32 220
Fragments arranged by size:
549 220 219 174 54 32 9
NspI r_CATG'y
Cuts at: 0 612 1081 1240 1257
Size: 612 469 159 17
Fragments arranged by size:
612 469 159 17
PciI A'CATG_T
Cuts at: 0 1236 1257
Size: 1236 21
PflMI CCAn_nnn'nTGG
Cuts at: 0 507 624 1257
Size: 507 117 633
Fragments arranged by size:
633 507 117
PfoI T'CCnGG_A
Cuts at: 0 290 1257
Size: 290 967
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 256 288 1257
Size: 256 32 969
Fragments arranged by size:
969 256 32
PspOMI G'GGCC_C
Cuts at: 0 518 1257
Size: 518 739
PstI C_TGCA'G
Cuts at: 0 461 575 989 1010 1232 1257
Size: 461 114 414 21 222 25
Fragments arranged by size:
461 414 222 114 25 21
PvuII CAG'CTG
Cuts at: 0 549 603 777 996 1005 1037 1257
Size: 549 54 174 219 9 32 220
Fragments arranged by size:
549 220 219 174 54 32 9
SacI G_AGCT'C
Cuts at: 0 182 983 1257
Size: 182 801 274
Fragments arranged by size:
801 274 182
SapI GCTCTTCn'nnn_
Cuts at: 0 13 715 1257
Size: 13 702 542
Fragments arranged by size:
702 542 13
SbfI CC_TGCA'GG
Cuts at: 0 989 1257
Size: 989 268
SfcI C'TryA_G
Cuts at: 0 457 571 985 1006 1228 1257
Size: 457 114 414 21 222 29
Fragments arranged by size:
457 414 222 114 29 21
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 526 814 823 1257
Size: 526 288 9 434
Fragments arranged by size:
526 434 288 9
SmaI CCC'GGG
Cuts at: 0 523 955 1257
Size: 523 432 302
Fragments arranged by size:
523 432 302
SphI G_CATG'C
Cuts at: 0 612 1257
Size: 612 645
SrfI GCCC'GGGC
Cuts at: 0 523 1257
Size: 523 734
StuI AGG'CCT
Cuts at: 0 418 1257
Size: 418 839
TatI w'GTAC_w
Cuts at: 0 1016 1257
Size: 1016 241
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 306 1257
Size: 306 951
XmaI C'CCGG_G
Cuts at: 0 521 953 1257
Size: 521 432 304
Fragments arranged by size:
521 432 304
XmnI GAAnn'nnTTC
Cuts at: 0 271 1257
Size: 271 986
ZraI GAC'GTC
Cuts at: 0 440 1257
Size: 440 817
Enzymes that do cut:
AarI AatII AcuI AfeI AflIII AloI AlwNI ApaI
AvaI BanI BanII BbvCI BglI BglII Bme1580I BmrI
BpmI Bpu10I BsaI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspMI BsrBI BstAPI BstXI
BstYI BtsI ClaI DraIII EaeI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRV FalI FspI HaeII Hin4I MscI
MspA1I NspI PciI PflMI PfoI PpiI PspOMI PstI
PvuII SacI SapI SbfI SfcI SfiI SmaI SphI
SrfI StuI TatI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AgeI AhdI AleI AlfI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BbsI BcgI BciVI BclI BfrBI BlpI BmgBI
BmtI BplI BpuEI BsaAI BsaBI BsiEI BsiWI BsmBI
BspEI BspHI BsrDI BsrFI BsrGI BssHII BssSI BstBI
BstEII BstZ17I Bsu36I BtgI BtgZI CspCI DraI DrdI
EagI EcoNI EcoRI FseI FspAI HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MslI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PmeI PmlI PpuMI PshAI PsiI PspXI PsrI
PvuI RsrII SacII SalI SanDI ScaI SexAI SfoI
SgfI SgrAI SmlI SnaBI SpeI SspI StyI SwaI
TaqII TstI Tth111I XbaI XhoI