Restriction map for
pFN21AB5455
(Circular) (Six-base) MAPSORT of: pFN21AB5455.seq Check: 1096 from: 1 to: 5830
pFN21AB5455 5830 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3640 278
Size: 53 83 186 936 2104 2468
Fragments arranged by size:
2468 2104 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2034 3173 1937
Size: 97 1139 4594
Fragments arranged by size:
4594 1139 97
Acc65I G'GTAC_C
Cuts at: 3151 3151
Size: 5830
AclI AA'CG_TT
Cuts at: 1563 3957 4330 1563
Size: 2394 373 3063
Fragments arranged by size:
3063 2394 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2225 3893 5277 5545 1632
Size: 593 1668 1384 268 1917
Fragments arranged by size:
1917 1668 1384 593 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5633
AflIII A'CryG_T
Cuts at: 1203 4729 5706 1203
Size: 3526 977 1327
Fragments arranged by size:
3526 1327 977
AgeI A'CCGG_T
Cuts at: 4661 4661
Size: 5830
AhdI GACnn_n'nnGTC
Cuts at: 4559 4559
Size: 5830
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5830
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5830
AlwNI CAG_nnn'CTG
Cuts at: 3573 5145 3573
Size: 1572 4258
ApaLI G'TGCA_C
Cuts at: 3887 5043 3887
Size: 1156 4674
ApoI r'AATT_y
Cuts at: 1493 3139 3365 3517 5626 1493
Size: 1646 226 152 2109 1697
Fragments arranged by size:
2109 1697 1646 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4384 160
Size: 872 3352 1606
Fragments arranged by size:
3352 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5830
AvaI C'yCGr_G
Cuts at: 1870 1943 2085 2297 3155 1870
Size: 73 142 212 858 4545
Fragments arranged by size:
4545 858 212 142 73
BamHI G'GATC_C
Cuts at: 3160 3527 3160
Size: 367 5463
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2112 2121 2262 2909 2965
Size: 325 217 627 325 9 141 647 56
Cuts at: 2965 3151 4606 618
Size: 186 1455 1842
Fragments arranged by size:
1842 1455 647 627 325 325 217 186
141 56 9
BanII G_rGCy'C
Cuts at: 1391 3149 1391
Size: 1758 4072
BbeI G_GCGC'C
Cuts at: 2116 2116
Size: 5830
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2086 928
Size: 971 187 4672
Fragments arranged by size:
4672 971 187
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2492 2526 3109 3143 4021 4055 1903
Size: 34 555 34 583 34 878 34 3678
Fragments arranged by size:
3678 878 583 555 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3722 4932 3722
Size: 1210 4620
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5830
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2087 4441 136
Size: 107 122 71 631 605 415 2354 1525
Fragments arranged by size:
2354 1525 631 605 415 122 107 71
BglII A'GATC_T
Cuts at: 5825 5825
Size: 5830
BlpI GC'TnA_GC
Cuts at: 3247 3247
Size: 5830
Bme1580I G_kGCm'C
Cuts at: 1790 2496 3891 5047 1790
Size: 706 1395 1156 2573
Fragments arranged by size:
2573 1395 1156 706
BmgBI CAC'GTC
Cuts at: 1318 2863 1318
Size: 1545 4285
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2248 3561 4519
Size: 416 53 83 399 24 831 1313 958
Cuts at: 4519 442
Size: 1753
Fragments arranged by size:
1753 1313 958 831 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5830
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2213 2213
Size: 5830
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2732 4490 1239
Size: 1493 1758 2579
Fragments arranged by size:
2579 1758 1493
Bpu10I CC'TnA_GC
Cuts at: 3003 3003
Size: 5830
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3955 4820 5118 3955
Size: 865 298 4667
Fragments arranged by size:
4667 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2372 4493 882
Size: 617 873 2121 2219
Fragments arranged by size:
2219 2121 873 617
BsaAI yAC'GTr
Cuts at: 493 1204 3621 493
Size: 711 2417 2702
Fragments arranged by size:
2702 2417 711
BsaBI GATnn'nnATC
Cuts at: 2987 3071 5824 2987
Size: 84 2753 2993
Fragments arranged by size:
2993 2753 84
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2113 3637 4019 4654
Size: 53 83 186 936 580 1524 382 635
Cuts at: 4654 275
Size: 1451
Fragments arranged by size:
1524 1451 936 635 580 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2468 4263 4661 4935 5082 5493 2468
Size: 1795 398 274 147 411 2805
Fragments arranged by size:
2805 1795 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2027 2057 2636 2666 1178
Size: 30 165 30 624 30 579 30 4342
Fragments arranged by size:
4342 624 579 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2315 2695 2988 1186
Size: 1129 380 293 4028
Fragments arranged by size:
4028 1129 380 293
BseYI C'CCAG_C
Cuts at: 2392 2969 5033 5529 2392
Size: 577 2064 496 2693
Fragments arranged by size:
2693 2064 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5257
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2616 3280 4041 5069 1540
Size: 275 186 615 664 761 1028 2301
Fragments arranged by size:
2301 1028 761 664 615 275 186
BsiHKAI G_wGCw'C
Cuts at: 2223 2307 3891 3976 5047 2223
Size: 84 1584 85 1071 3006
Fragments arranged by size:
3006 1584 1071 85 84
BsmI GAATG_Cn'
Cuts at: 3341 3434 3341
Size: 93 5737
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2223 2307 2496 3149 3891 3976 5047
Size: 399 433 84 189 653 742 85 1071
Cuts at: 5047 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 653 433 399 189 85
84
BspEI T'CCGG_A
Cuts at: 5493 5493
Size: 5830
BspHI T'CATG_A
Cuts at: 2571 2850 3719 5449 2571
Size: 279 869 1730 2952
Fragments arranged by size:
2952 1730 869 279
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3185 844
Size: 2341 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2620 2764 3717 1394
Size: 429 797 144 953 3507
Fragments arranged by size:
3507 953 797 429 144
BsrDI GCAATG_nn'
Cuts at: 65 1248 2764 2928 2991 4325 4499 65
Size: 1183 1516 164 63 1334 174 1396
Fragments arranged by size:
1516 1396 1334 1183 174 164 63
BsrFI r'CCGG_y
Cuts at: 1700 1810 2060 4474 4661 1700
Size: 110 250 2414 187 2869
Fragments arranged by size:
2869 2414 250 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5830
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5830
BssSI C'ACGA_G
Cuts at: 3890 4902 3890
Size: 1012 4818
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2311 794
Size: 1517 4313
BstBI TT'CG_AA
Cuts at: 4634 4634
Size: 5830
BstEII G'GTnAC_C
Cuts at: 1184 2479 1184
Size: 1295 4535
BstYI r'GATC_y
Cuts at: 1972 2577 3160 3527 3912 3929 5381 5467 5479
Size: 605 583 367 385 17 1452 86 12
Cuts at: 5479 5825 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 605 583 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2778 2778
Size: 5830
BtgI C'CryG_G
Cuts at: 513 1065 2004 2610 2688 513
Size: 552 939 606 78 3655
Fragments arranged by size:
3655 939 606 552 78
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5091
Fragments arranged by size:
5091 683 56
BtsI GCAGTG_nn'
Cuts at: 2955 3346 4140 4160 2955
Size: 391 794 20 4625
Fragments arranged by size:
4625 794 391 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5830
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5795
DraI TTT'AAA
Cuts at: 3134 3134
Size: 5830
DraIII CAC_nnn'GTG
Cuts at: 2311 2491 2311
Size: 180 5650
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2741 4837 809
Size: 507 1425 2096 1802
Fragments arranged by size:
2096 1802 1425 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2440 2548 2613
Size: 54 1425 50 275 18 610 108 65
Cuts at: 2613 2685 2737 3277 4166 5795 8
Size: 72 52 540 889 1629 43
Fragments arranged by size:
1629 1425 889 610 540 275 108 72
65 54 52 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2613 3277 1537
Size: 275 801 664 4090
Fragments arranged by size:
4090 801 664 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2711 2792 3760 1353
Size: 1358 81 968 3423
Fragments arranged by size:
3423 1358 968 81
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4417 4791 4937 387
Size: 1064 138 2828 374 146 1280
Fragments arranged by size:
2828 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2225 2732 3893 4490 5277 5545 1239
Size: 393 593 507 1161 597 787 268 1524
Fragments arranged by size:
1524 1161 787 597 593 507 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2326 2554 2326
Size: 228 5602
EcoRI G'AATT_C
Cuts at: 3139 3517 3139
Size: 378 5452
EcoRV GAT'ATC
Cuts at: 2017 2017
Size: 5830
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2080 2080
Size: 5830
FspI TGC'GCA
Cuts at: 4336 4336
Size: 5830
HaeII r_GCGC'y
Cuts at: 1678 2116 4977 1678
Size: 438 2861 2531
Fragments arranged by size:
2861 2531 438
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2027 2059 2980 3012 4471
Size: 32 135 32 455 32 921 32 1459
Cuts at: 4471 4503 4545 4577 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1459 921 455 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2035 3174 3420 669
Size: 988 281 97 1139 246 3079
Fragments arranged by size:
3079 1139 988 281 246 97
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5830
HpaI GTT'AAC
Cuts at: 3420 3420
Size: 5830
KasI G'GCGC_C
Cuts at: 2112 2112
Size: 5830
KpnI G_GTAC'C
Cuts at: 3155 3155
Size: 5830
MfeI C'AATT_G
Cuts at: 3429 3429
Size: 5830
MluI A'CGCG_T
Cuts at: 5706 5706
Size: 5830
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4944 5128 1305
Size: 3639 184 2007
Fragments arranged by size:
3639 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2687 10
Size: 54 1425 343 855 3153
Fragments arranged by size:
3153 1425 855 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2459 2915 3788 4147 4306 518
Size: 648 1293 456 873 359 159 2042
Fragments arranged by size:
2042 1293 873 648 456 359 159
MspA1I CmG'CkG
Cuts at: 1606 2772 3246 3923 5071 5316 1606
Size: 1166 474 677 1148 245 2120
Fragments arranged by size:
2120 1166 1148 677 474 245
NaeI GCC'GGC
Cuts at: 2062 2062
Size: 5830
NarI GG'CG_CC
Cuts at: 2113 2113
Size: 5830
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4339
Fragments arranged by size:
4339 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5830
NgoMIV G'CCGG_C
Cuts at: 2060 2060
Size: 5830
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5830
NotI GC'GGCC_GC
Cuts at: 3277 3277
Size: 5830
NspI r_CATG'y
Cuts at: 1132 1229 2053 3188 4733 1132
Size: 97 824 1135 1545 2229
Fragments arranged by size:
2229 1545 1135 824 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5830
PciI A'CATG_T
Cuts at: 4729 4729
Size: 5830
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5830
PmeI GTTT'AAAC
Cuts at: 3134 3134
Size: 5830
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3899 3931 5412 5444 3899
Size: 32 1481 32 4285
Fragments arranged by size:
4285 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2554 2554
Size: 5830
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5830
PsiI TTA'TAA
Cuts at: 3400 3400
Size: 5830
PstI C_TGCA'G
Cuts at: 830 3182 830
Size: 2352 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5830
SalI G'TCGA_C
Cuts at: 1936 2033 3172 1936
Size: 97 1139 4594
Fragments arranged by size:
4594 1139 97
SapI GCTCTTCn'nnn_
Cuts at: 2711 2711
Size: 5830
SbfI CC_TGCA'GG
Cuts at: 3182 3182
Size: 5830
ScaI AGT'ACT
Cuts at: 3014 4078 5790 3014
Size: 1064 1712 3054
Fragments arranged by size:
3054 1712 1064
SfcI C'TryA_G
Cuts at: 826 1045 2596 3178 4313 4994 5185 826
Size: 219 1551 582 1135 681 191 1471
Fragments arranged by size:
1551 1471 1135 681 582 219 191
SfoI GGC'GCC
Cuts at: 2114 2114
Size: 5830
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5830
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5830
SmaI CCC'GGG
Cuts at: 1872 2087 3157 1872
Size: 215 1070 4545
Fragments arranged by size:
4545 1070 215
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3934 4835 5097 820
Size: 197 926 1991 901 262 1553
Fragments arranged by size:
1991 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3621 493
Size: 3128 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5830
SphI G_CATG'C
Cuts at: 1132 3188 1132
Size: 2056 3774
SspI AAT'ATT
Cuts at: 5 52 3754 5
Size: 47 3702 2081
Fragments arranged by size:
3702 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5830
StyI C'CwwG_G
Cuts at: 513 1065 2004 2340 3269 513
Size: 552 939 336 929 3074
Fragments arranged by size:
3074 939 929 552 336
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2336 2498 2983 3857 3874 4027 4212
Size: 1255 756 162 485 874 17 153 185
Cuts at: 4212 4657 325
Size: 445 1498
Fragments arranged by size:
1498 1255 874 756 485 445 185 162
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3012 4076 5788
Size: 274 80 33 51 1443 1035 1064 1712
Cuts at: 5788 96
Size: 138
Fragments arranged by size:
1712 1443 1064 1035 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2636 2668 5790 5822 2636
Size: 32 3122 32 2644
Fragments arranged by size:
3122 2644 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2477 1316
Size: 1161 4669
XbaI T'CTAG_A
Cuts at: 3166 3166
Size: 5830
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2533 2707 2533
Size: 174 5656
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5830
XmaI C'CCGG_G
Cuts at: 1870 2085 3155 1870
Size: 215 1070 4545
Fragments arranged by size:
4545 1070 215
XmnI GAAnn'nnTTC
Cuts at: 3959 3959
Size: 5830
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3638 276
Size: 53 83 186 936 2104 2468
Fragments arranged by size:
2468 2104 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI FseI
FspAI NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI
SrfI SwaI