Restriction map for
pFN21AB5438
##################################
# NO CUT ENZYMES ( 118 enzymes) #
##################################
Acc65I AccIII AclI AflII AflIII
AgeI AhdI Aor51HI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BanI BbeI BbvCI
BcgI BciVI BclI BfaI BfrI
BlnI BlpI Bpu1102I BsaWI BseAI
BsiEI BsiWI BsmI BspDI BspEI
BspLU11I BspMI BsrBI BssHII BssSI
Bst1107I BstAPI BstBI BstXI BstZ17I
CelII ClaI CpoI DraI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HindIII HpaI
KasI KpnI KspI MaeI MfeI
MluI MroI MseI MunI NarI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspI StyI SwaI
Tru9I Tth111I XbaI XcmI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 337 GACGTC GACGT^C
AcsI 298 AAATTC [GA]^AATT[CT]
Alw44I 348 GTGCAC G^TGCAC
AlwNI 826 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 702 CCTGAGG CC^T[ACGT]AGG
ApaI 695 GGGCCC GGGCC^C
ApaLI 348 GTGCAC G^TGCAC
ApoI 298 AAATTC [GA]^AATT[CT]
AspHI 348 GTGCAC G[AT]GC[AT]^C
BalI 404 TGGCCA TGG^CCA
BamHI 3 GGATCC G^GATCC
BbrPI 1230 CACGTG CAC^GTG
BbsI 145 GTCTTC GAAGAC(2/6)
BglI 550 GCCAACCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 145 GTCTTC GAAGAC(2/6)
BsaI 937 GGTCTC GGTCTC(1/5)
BsgI 753 CTGCAC GTGCAG(16/14)
BsiHKAI 348 GTGCAC G[AT]GC[AT]^C
BsmBI 582 CGTCTC CGTCTC(1/5)
BsrDI 546 CATTGC GCAATG(2/0)
BsrFI 56 GCCGGC [GA]^CCGG[CT]
BstEII 450 GGTCACC G^GT[ACGT]ACC
BstPI 450 GGTCACC G^GT[ACGT]ACC
Bsu36I 702 CCTGAGG CC^T[ACGT]AGG
Cfr10I 56 GCCGGC [GA]^CCGG[CT]
DsaI 933 CCACGG C^C[GA][CT]GG
EarI 508 CTCTTC CTCTTC(1/4)
Eco81I 702 CCTGAGG CC^T[ACGT]AGG
EcoO65I 450 GGTCACC G^GT[ACGT]ACC
HgaI 650 GACGC GACGC(5/10)
Ksp632I 508 CTCTTC CTCTTC(1/4)
MluNI 404 TGGCCA TGG^CCA
MscI 404 TGGCCA TGG^CCA
NaeI 56 GCCGGC GCC^GGC
NgoMIV 56 GCCGGC G^CCGGC
NspI 984 GCATGC [GA]CATG^[CT]
PmaCI 1230 CACGTG CAC^GTG
PmlI 1230 CACGTG CAC^GTG
PstI 193 CTGCAG CTGCA^G
SmlI 1060 CTCAAG C^T[CT][GA]AG
SphI 984 GCATGC GCATG^C
StuI 1301 AGGCCT AGG^CCT
TaqI 386 TCGA T^CGA
Tsp509I 299 AATT ^AATT
TthHB8I 386 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 181 CGCG CG^CG
428 CGCG
AcyI 337 GACGTC G[GA]^CG[CT]C
650 GACGCC
AvaI 736 CCCGAG C^[CT]CG[GA]G
865 CCCGAG
BglII 629 AGATCT A^GATCT
1013 AGATCT
BsaAI 592 TACGTG [CT]AC^GT[GA]
1230 CACGTG
BsaBI 28 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
462 GATCTCCATC
BsaHI 337 GACGTC G[GA]^CG[CT]C
650 GACGCC
BsmAI 583 GTCTC GTCTC(1/5)
938 GTCTC
BsmFI 177 GTCCC GGGAC(10/14)
927 GTCCC
BsoBI 736 CCCGAG C^[CT]CG[GA]G
865 CCCGAG
Bsp1407I 419 TGTACA T^GTACA
1217 TGTACA
BsrGI 419 TGTACA T^GTACA
1217 TGTACA
BstUI 181 CGCG CG^CG
428 CGCG
DdeI 703 CTGAG C^T[ACGT]AG
1112 CTCAG
DrdI 238 GACCTCCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
319 GACATCTTCGTC
EaeI 233 TGGCCG [CT]^GGCC[GA]
404 TGGCCA
Hin1I 337 GACGTC G[GA]^CG[CT]C
650 GACGCC
HincII 664 GTCAAC GT[CT]^[GA]AC
1246 GTCAAC
HindII 664 GTCAAC GT[CT]^[GA]AC
1246 GTCAAC
MamI 28 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
462 GATCTCCATC
MspA1I 398 CAGCTG C[AC]G^C[GT]G
907 CAGCTG
MvnI 181 CGCG CG^CG
428 CGCG
PflMI 467 CCATCGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
825 CCAGGAGCTGG
PpuMI 476 GGGTCCT [GA]G^G[AT]CC[CT]
871 AGGACCC
PvuII 398 CAGCTG CAG^CTG
907 CAGCTG
SfcI 193 CTGCAG C^T[GA][CT]AG
432 CTACAG
SspBI 419 TGTACA T^GTACA
1217 TGTACA
TfiI 12 GAATC G^A[AT]TC
309 GATTC
Van91I 467 CCATCGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
825 CCAGGAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AccI AciI AfaI AluI AlwI
AvaII BanII BbvI BcnI BmyI
BpmI BsaJI BseRI BsiYI BslI
Bsp1286I BspHI BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DpnII DraII EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MflI MnlI MslI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI TseI Tsp45I TspRI
XhoII