(Circular) (Six-base) MAPSORT of: pFN21AB5438.seq Check: 9053 from: 1 to: 6031 pFN21AB5438 6031 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2346 3841 278 Size: 53 83 186 936 810 1495 2468 Fragments arranged by size: 2468 1495 936 810 186 83 53 AccI GT'mk_AC Cuts at: 1937 2280 2574 2625 3374 1937 Size: 343 294 51 749 4594 Fragments arranged by size: 4594 749 343 294 51 Acc65I G'GTAC_C Cuts at: 3352 3352 Size: 6031 AclI AA'CG_TT Cuts at: 1563 4158 4531 1563 Size: 2595 373 3063 Fragments arranged by size: 3063 2595 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3335 4094 5478 5746 1632 Size: 1703 759 1384 268 1917 Fragments arranged by size: 1917 1703 1384 759 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5834 AflIII A'CryG_T Cuts at: 1203 4930 5907 1203 Size: 3727 977 1327 Fragments arranged by size: 3727 1327 977 AgeI A'CCGG_T Cuts at: 4862 4862 Size: 6031 AhdI GACnn_n'nnGTC Cuts at: 4760 4760 Size: 6031 AleI CACnn'nnGTG Cuts at: 1166 2405 1166 Size: 1239 4792 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6031 AlwNI CAG_nnn'CTG Cuts at: 2836 3774 5346 2836 Size: 938 1572 3521 Fragments arranged by size: 3521 1572 938 ApaI G_GGCC'C Cuts at: 2704 2704 Size: 6031 ApaLI G'TGCA_C Cuts at: 2353 4088 5244 2353 Size: 1735 1156 3140 Fragments arranged by size: 3140 1735 1156 ApoI r'AATT_y Cuts at: 1493 2303 3340 3566 3718 5827 1493 Size: 810 1037 226 152 2109 1697 Fragments arranged by size: 2109 1697 1037 810 226 152 AseI AT'TA_AT Cuts at: 160 1032 4585 160 Size: 872 3553 1606 Fragments arranged by size: 3553 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6031 AvaI C'yCGr_G Cuts at: 1870 1943 2741 2870 3356 1870 Size: 73 798 129 486 4545 Fragments arranged by size: 4545 798 486 129 73 BamHI G'GATC_C Cuts at: 2008 3361 3728 2008 Size: 1353 367 4311 Fragments arranged by size: 4311 1353 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3352 4807 618 Size: 325 217 627 1565 1455 1842 Fragments arranged by size: 1842 1565 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2704 2716 2830 3350 1391 Size: 1313 12 114 520 4072 Fragments arranged by size: 4072 1313 520 114 12 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2143 928 Size: 971 244 4816 Fragments arranged by size: 4816 971 244 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4222 4256 1903 Size: 34 2285 34 3678 Fragments arranged by size: 3678 2285 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3923 5133 3923 Size: 1210 4821 BclI T'GATC_A Cuts at: 1548 1548 Size: 6031 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2561 4642 136 Size: 107 122 71 631 605 889 2081 1525 Fragments arranged by size: 2081 1525 889 631 605 122 107 71 BglII A'GATC_T Cuts at: 2634 3018 6026 2634 Size: 384 3008 2639 Fragments arranged by size: 3008 2639 384 BlpI GC'TnA_GC Cuts at: 3448 3448 Size: 6031 Bme1580I G_kGCm'C Cuts at: 1790 2357 2604 2704 4092 5248 1790 Size: 567 247 100 1388 1156 2573 Fragments arranged by size: 2573 1388 1156 567 247 100 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6031 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2786 3762 4720 Size: 416 53 83 399 24 1369 976 958 Cuts at: 4720 442 Size: 1753 Fragments arranged by size: 1753 1369 976 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6031 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2869 2869 Size: 6031 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2063 2077 2306 2839 2858 4691 1239 Size: 824 14 229 533 19 1833 2579 Fragments arranged by size: 2579 1833 824 533 229 19 14 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3050 4156 5021 5319 3050 Size: 1106 865 298 3762 Fragments arranged by size: 3762 1106 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2948 4694 882 Size: 617 1449 1746 2219 Fragments arranged by size: 2219 1746 1449 617 BsaAI yAC'GTr Cuts at: 493 1204 2599 3237 3822 493 Size: 711 1395 638 585 2702 Fragments arranged by size: 2702 1395 711 638 585 BsaBI GATnn'nnATC Cuts at: 2037 2471 6025 2037 Size: 434 3554 2043 Fragments arranged by size: 3554 2043 434 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2343 2656 3838 4220 Size: 53 83 186 936 810 313 1182 382 Cuts at: 4220 4855 275 Size: 635 1451 Fragments arranged by size: 1451 1182 936 810 635 382 313 186 83 53 BsaWI w'CCGG_w Cuts at: 4464 4862 5136 5283 5694 4464 Size: 398 274 147 411 4801 Fragments arranged by size: 4801 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5806 Fragments arranged by size: 5806 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2237 2501 2569 1186 Size: 1051 264 68 4648 Fragments arranged by size: 4648 1051 264 68 BseYI C'CCAG_C Cuts at: 2900 3093 3241 5234 5730 2900 Size: 193 148 1993 496 3201 Fragments arranged by size: 3201 1993 496 193 148 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2743 1163 Size: 573 1007 4451 Fragments arranged by size: 4451 1007 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3481 4242 5270 1540 Size: 275 186 1480 761 1028 2301 Fragments arranged by size: 2301 1480 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2357 4092 4177 5248 2357 Size: 1735 85 1071 3140 Fragments arranged by size: 3140 1735 1071 85 BsmI GAATG_Cn' Cuts at: 3542 3635 3542 Size: 93 5938 BsmBI CGTCTCn'nnnn_ Cuts at: 2593 2593 Size: 6031 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2357 2604 2704 2716 2830 3350 4092 Size: 399 567 247 100 12 114 520 742 Cuts at: 4092 4177 5248 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 567 520 399 247 114 100 85 12 BspEI T'CCGG_A Cuts at: 5694 5694 Size: 6031 BspHI T'CATG_A Cuts at: 2346 2463 2769 3920 5650 2346 Size: 117 306 1151 1730 2727 Fragments arranged by size: 2727 1730 1151 306 117 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3386 844 Size: 2542 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3918 1394 Size: 429 2095 3507 Fragments arranged by size: 3507 2095 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2550 4526 4700 65 Size: 1183 1302 1976 174 1396 Fragments arranged by size: 1976 1396 1302 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2061 4675 4862 1700 Size: 110 251 2614 187 2869 Fragments arranged by size: 2869 2614 251 187 110 BsrGI T'GTAC_A Cuts at: 96 2424 3222 96 Size: 2328 798 2905 Fragments arranged by size: 2905 2328 798 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6031 BssSI C'ACGA_G Cuts at: 4091 5103 4091 Size: 1012 5019 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6031 BstBI TT'CG_AA Cuts at: 4835 4835 Size: 6031 BstEII G'GTnAC_C Cuts at: 1184 2455 1184 Size: 1271 4760 BstYI r'GATC_y Cuts at: 1972 2008 2634 3018 3027 3318 3361 3728 4113 Size: 36 626 384 9 291 43 367 385 Cuts at: 4113 4130 5582 5668 5680 6026 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 626 385 384 367 346 291 86 43 36 17 12 9 Bsu36I CC'TnA_GG Cuts at: 2708 2708 Size: 6031 BtgI C'CryG_G Cuts at: 513 1065 2004 2938 513 Size: 552 939 934 3606 Fragments arranged by size: 3606 939 934 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2122 487 Size: 683 56 896 4396 Fragments arranged by size: 4396 896 683 56 BtsI GCAGTG_nn' Cuts at: 2120 2241 3325 3547 4341 4361 2120 Size: 121 1084 222 794 20 3790 Fragments arranged by size: 3790 1084 794 222 121 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6031 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5996 DraI TTT'AAA Cuts at: 3335 3335 Size: 6031 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2249 2330 5038 809 Size: 507 933 81 2708 1802 Fragments arranged by size: 2708 1802 933 507 81 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2238 2409 3478 Size: 54 1425 50 275 18 408 171 1069 Cuts at: 3478 4367 5996 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1069 889 408 275 171 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3478 1537 Size: 275 1666 4090 Fragments arranged by size: 4090 1666 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2519 3961 1353 Size: 1166 1442 3423 Fragments arranged by size: 3423 1442 1166 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2105 2818 4618 4992 5138 387 Size: 1064 138 516 713 1800 374 146 1280 Fragments arranged by size: 1800 1280 1064 713 516 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2063 2077 2306 2839 2858 3335 4094 Size: 393 431 14 229 533 19 477 759 Cuts at: 4094 4691 5478 5746 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 787 759 597 533 477 431 393 268 229 19 14 EcoO109I rG'GnC_Cy Cuts at: 2482 2700 2877 2482 Size: 218 177 5636 Fragments arranged by size: 5636 218 177 EcoRI G'AATT_C Cuts at: 3340 3718 3340 Size: 378 5653 FspI TGC'GCA Cuts at: 4537 4537 Size: 6031 HaeII r_GCGC'y Cuts at: 1678 2198 2452 3231 3261 5178 1678 Size: 520 254 779 30 1917 2531 Fragments arranged by size: 2531 1917 779 520 254 30 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4672 4704 4746 4778 1373 Size: 32 135 32 3100 32 42 32 2626 Fragments arranged by size: 3100 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2671 3253 3375 3621 669 Size: 988 281 733 582 122 246 3079 Fragments arranged by size: 3079 988 733 582 281 246 122 HindIII A'AGCT_T Cuts at: 748 748 Size: 6031 HpaI GTT'AAC Cuts at: 3621 3621 Size: 6031 KpnI G_GTAC'C Cuts at: 3356 3356 Size: 6031 MfeI C'AATT_G Cuts at: 3630 3630 Size: 6031 MluI A'CGCG_T Cuts at: 5907 5907 Size: 6031 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5145 5329 1305 Size: 3840 184 2007 Fragments arranged by size: 3840 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2411 10 Size: 54 1425 343 579 3630 Fragments arranged by size: 3630 1425 579 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2177 2405 2417 2462 3119 3989 4348 Size: 648 1011 228 12 45 657 870 359 Cuts at: 4348 4507 518 Size: 159 2042 Fragments arranged by size: 2042 1011 870 657 648 359 228 159 45 12 MspA1I CmG'CkG Cuts at: 1606 2405 2914 3447 4124 5272 5517 1606 Size: 799 509 533 677 1148 245 2120 Fragments arranged by size: 2120 1148 799 677 533 509 245 NaeI GCC'GGC Cuts at: 2063 2063 Size: 6031 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4540 Fragments arranged by size: 4540 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6031 NgoMIV G'CCGG_C Cuts at: 2061 2061 Size: 6031 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6031 NotI GC'GGCC_GC Cuts at: 3478 3478 Size: 6031 NspI r_CATG'y Cuts at: 1132 1229 2993 3389 4934 1132 Size: 97 1764 396 1545 2229 Fragments arranged by size: 2229 1764 1545 396 97 PasI CC'CwG_GG Cuts at: 1348 2271 1348 Size: 923 5108 PciI A'CATG_T Cuts at: 4930 4930 Size: 6031 PflMI CCAn_nnn'nTGG Cuts at: 1108 2478 2836 1108 Size: 1370 358 4303 Fragments arranged by size: 4303 1370 358 PmeI GTTT'AAAC Cuts at: 3335 3335 Size: 6031 PmlI CAC'GTG Cuts at: 3237 3237 Size: 6031 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4100 4132 5613 5645 4100 Size: 32 1481 32 4486 Fragments arranged by size: 4486 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2482 2877 2482 Size: 395 5636 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6031 PsiI TTA'TAA Cuts at: 3601 3601 Size: 6031 PspOMI G'GGCC_C Cuts at: 2700 2700 Size: 6031 PstI C_TGCA'G Cuts at: 830 2202 3383 830 Size: 1372 1181 3478 Fragments arranged by size: 3478 1372 1181 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6031 PvuII CAG'CTG Cuts at: 2405 2914 2405 Size: 509 5522 SalI G'TCGA_C Cuts at: 1936 3373 1936 Size: 1437 4594 SbfI CC_TGCA'GG Cuts at: 3383 3383 Size: 6031 ScaI AGT'ACT Cuts at: 4279 5991 4279 Size: 1712 4319 SfcI C'TryA_G Cuts at: 826 1045 2198 2437 3379 4514 5195 5386 826 Size: 219 1153 239 942 1135 681 191 1471 Fragments arranged by size: 1471 1153 1135 942 681 239 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6031 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6031 SmaI CCC'GGG Cuts at: 1872 3358 1872 Size: 1486 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3065 4135 5036 5298 820 Size: 197 926 1122 1070 901 262 1553 Fragments arranged by size: 1553 1122 1070 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3822 493 Size: 3329 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6031 SphI G_CATG'C Cuts at: 1132 2993 3389 1132 Size: 1861 396 3774 Fragments arranged by size: 3774 1861 396 SspI AAT'ATT Cuts at: 5 52 3955 5 Size: 47 3903 2081 Fragments arranged by size: 3903 2081 47 StuI AGG'CCT Cuts at: 1517 3308 1517 Size: 1791 4240 StyI C'CwwG_G Cuts at: 513 1065 2004 3470 513 Size: 552 939 1466 3074 Fragments arranged by size: 3074 1466 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2914 4058 4075 4228 4413 4858 325 Size: 1255 1334 1144 17 153 185 445 1498 Fragments arranged by size: 1498 1334 1255 1144 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2424 2732 3222 Size: 274 80 33 51 1443 447 308 490 Cuts at: 3222 4277 5989 96 Size: 1055 1712 138 Fragments arranged by size: 1712 1443 1055 490 447 308 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2305 2337 5991 6023 2305 Size: 32 3654 32 2313 Fragments arranged by size: 3654 2313 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6031 XbaI T'CTAG_A Cuts at: 3367 3367 Size: 6031 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6031 XmaI C'CCGG_G Cuts at: 1870 3356 1870 Size: 1486 4545 XmnI GAAnn'nnTTC Cuts at: 4160 4160 Size: 6031 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2344 3839 276 Size: 53 83 186 936 810 1495 2468 Fragments arranged by size: 2468 1495 936 810 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI