Restriction map for
pFN21AB5398
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# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcsI AflIII AgeI Alw44I AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp718I AspI AvaI
AvrII BamHI BbrPI BbvCI BcgI
BciVI BclI BfaI BglII BlnI
BlpI BpmI Bpu1102I BsaAI BsaBI
BsaI BsiWI BsmAI BsmBI BsoBI
Bsp1407I BspDI BspLU11I BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DdeI DraI DrdI Eco47III
Eco81I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FauI FbaI FseI HgaI
HincII HindII HinfI HpaI KpnI
KspI MaeI MamI MfeI MluI
MunI MvnI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StyI SwaI
TaqI TfiI Tsp509I Tth111I TthHB8I
Van91I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 67 enzymes) #
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AccIII 530 TCCGGA T^CCGGA
AcyI 233 GGCGCC G[GA]^CG[CT]C
AflII 109 CTTAAG C^TTAAG
AhdI 511 GACTGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 450 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 6 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 511 GACTGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 384 GGACC G^G[AT]CC
AviII 53 TGCGCA TGC^GCA
BalI 207 TGGCCA TGG^CCA
BanI 233 GGCGCC G^G[CT][GA]CC
BbeI 233 GGCGCC GGCGC^C
BbsI 87 GAAGAC GAAGAC(2/6)
BcnI 348 CCGGG CC^[GC]GG
BfrI 109 CTTAAG C^TTAAG
BglI 225 GCCCCTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 87 GAAGAC GAAGAC(2/6)
BsaHI 233 GGCGCC G[GA]^CG[CT]C
BsaWI 530 TCCGGA [AT]^CCGG[AT]
BseAI 530 TCCGGA T^CCGGA
BseRI 313 GAGGAG GAGGAG(10/8)
BsgI 433 CTGCAC GTGCAG(16/14)
BsiEI 407 CGGCCG CG[GA][CT]^CG
BsmFI 197 GGGAC GGGAC(10/14)
BsmI 402 GAATGC GAATGC(1/-1)
BspEI 530 TCCGGA T^CCGGA
BspHI 320 TCATGA T^CATGA
BspMI 472 ACCTGC ACCTGC(4/8)
BsrDI 281 GCAATG GCAATG(2/0)
BstYI 218 AGATCC [GA]^GATC[CT]
Cac8I 330 GCTGGC GC[ACGT]^[ACGT]GC
DraIII 189 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 164 CCGTGG C^C[GA][CT]GG
EagI 407 CGGCCG C^GGCCG
Eam1105I 511 GACTGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 22 CTCTTC CTCTTC(1/4)
EclXI 407 CGGCCG C^GGCCG
Eco52I 407 CGGCCG C^GGCCG
EcoNI 21 CCTCTTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 53 TGCGCA TGC^GCA
HaeII 233 GGCGCC [GA]GCGC^[CT]
Hin1I 233 GGCGCC G[GA]^CG[CT]C
HindIII 106 AAGCTT A^AGCTT
HphI 118 GGTGA GGTGA(8/7)
KasI 233 GGCGCC G^GCGCC
Ksp632I 22 CTCTTC CTCTTC(1/4)
MaeIII 127 GTGAC ^GT[ACGT]AC
MflI 218 AGATCC [GA]^GATC[CT]
MluNI 207 TGGCCA TGG^CCA
MroI 530 TCCGGA T^CCGGA
MscI 207 TGGCCA TGG^CCA
MseI 110 TTAA T^TAA
MspA1I 483 CAGCGG C[AC]G^C[GT]G
NarI 233 GGCGCC GG^CGCC
NciI 348 CCGGG CC^[GC]GG
PpuMI 383 AGGACCT [GA]G^G[AT]CC[CT]
PstI 80 CTGCAG CTGCA^G
RcaI 320 TCATGA T^CATGA
SfcI 80 CTGCAG C^T[GA][CT]AG
SfoI 233 GGCGCC GGC^GCC
SmlI 109 CTTAAG C^T[CT][GA]AG
StuI 258 AGGCCT AGG^CCT
Tru9I 110 TTAA T^TAA
Tsp45I 127 GTGAC ^GT[GC]AC
TspRI 189 CACTG CA[GC]TG[ACGT][ACGT]^
XhoII 218 AGATCC [GA]^GATC[CT]
XmnI 6 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AciI 406 GCGG CCGC(-3/-1)
485 GCGG
AfaI 171 GTAC GT^AC
459 GTAC
AlwI 96 GATCC GGATC(4/5)
219 GATCC
AspHI 316 GAGCTC G[AT]GC[AT]^C
343 GAGCTC
BbvI 79 GCTGC GCAGC(8/12)
432 GCTGC
BsaJI 164 CCGTGG C^C[ACGT][ACGT]GG
335 CCGAGG
BsiHKAI 316 GAGCTC G[AT]GC[AT]^C
343 GAGCTC
DraII 29 AGGGCCT [GA]G^G[ACGT]CC[CT]
383 AGGACCT
EcoO109I 29 AGGGCCT [GA]G^G[ACGT]CC[CT]
383 AGGACCT
MaeII 155 ACGT A^CGT
200 ACGT
MslI 57 CATCCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
321 CATGAGGATG
RsaI 171 GTAC GT^AC
459 GTAC
SacI 316 GAGCTC GAGCT^C
343 GAGCTC
TseI 79 GCTGC G^C[AT]GC
432 GCTGC
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AluI BanII BmyI BsiYI BslI
Bsp1286I BsrI BssKI BstF5I BstNI
CfoI Cfr13I DpnII EaeI EcoRII
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HpaII ItaI MboI MboII
MnlI MspI MvaI MwoI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
SfaNI