Restriction map for
pFN21AB5398
(Circular) (Six-base) MAPSORT of: pFN21AB5398.seq Check: 4723 from: 1 to: 5248
pFN21AB5398 5248 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2486 2486
Size: 5248
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3058 278
Size: 53 83 186 936 1522 2468
Fragments arranged by size:
2468 1522 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2591 1937
Size: 654 4594
Acc65I G'GTAC_C
Cuts at: 2569 2569
Size: 5248
AclI AA'CG_TT
Cuts at: 1563 3375 3748 1563
Size: 1812 373 3063
Fragments arranged by size:
3063 1812 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3311 4695 4963 1632
Size: 1679 1384 268 1917
Fragments arranged by size:
1917 1679 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2114 820
Size: 197 1097 3954
Fragments arranged by size:
3954 1097 197
AflIII A'CryG_T
Cuts at: 1203 4147 5124 1203
Size: 2944 977 1327
Fragments arranged by size:
2944 1327 977
AgeI A'CCGG_T
Cuts at: 4079 4079
Size: 5248
AhdI GACnn_n'nnGTC
Cuts at: 2521 3977 2521
Size: 1456 3792
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5248
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5248
AlwNI CAG_nnn'CTG
Cuts at: 2460 2991 4563 2460
Size: 531 1572 3145
Fragments arranged by size:
3145 1572 531
ApaLI G'TGCA_C
Cuts at: 3305 4461 3305
Size: 1156 4092
ApoI r'AATT_y
Cuts at: 1493 2557 2783 2935 5044 1493
Size: 1064 226 152 2109 1697
Fragments arranged by size:
2109 1697 1064 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3802 160
Size: 872 2770 1606
Fragments arranged by size:
2770 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5248
AvaI C'yCGr_G
Cuts at: 1870 1943 2573 1870
Size: 73 630 4545
Fragments arranged by size:
4545 630 73
BamHI G'GATC_C
Cuts at: 2578 2945 2578
Size: 367 4881
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2238 2569 4024 618
Size: 325 217 627 451 331 1455 1842
Fragments arranged by size:
1842 1455 627 451 331 325 217
BanII G_rGCy'C
Cuts at: 1391 2325 2352 2502 2567 1391
Size: 934 27 150 65 4072
Fragments arranged by size:
4072 934 150 65 27
BbeI G_GCGC'C
Cuts at: 2242 2242
Size: 5248
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2099 928
Size: 971 200 4077
Fragments arranged by size:
4077 971 200
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3439 3473 1903
Size: 34 1502 34 3678
Fragments arranged by size:
3678 1502 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3140 4350 3140
Size: 1210 4038
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5248
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2236 3859 136
Size: 107 122 71 631 605 564 1623 1525
Fragments arranged by size:
1623 1525 631 605 564 122 107 71
BglII A'GATC_T
Cuts at: 5243 5243
Size: 5248
BlpI GC'TnA_GC
Cuts at: 2665 2665
Size: 5248
Bme1580I G_kGCm'C
Cuts at: 1790 3309 4465 1790
Size: 1519 1156 2573
Fragments arranged by size:
2573 1519 1156
BmgBI CAC'GTC
Cuts at: 1318 2206 1318
Size: 888 4360
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2979 3937 442
Size: 416 53 83 399 24 1562 958 1753
Fragments arranged by size:
1753 1562 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5248
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3908 1239
Size: 2669 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3373 4238 4536 3373
Size: 865 298 4085
Fragments arranged by size:
4085 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3911 882
Size: 617 2412 2219
Fragments arranged by size:
2412 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3039 493
Size: 711 1835 2702
Fragments arranged by size:
2702 1835 711
BsaBI GATnn'nnATC
Cuts at: 5242 5242
Size: 5248
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2239 3055 3437 4072
Size: 53 83 186 936 706 816 382 635
Cuts at: 4072 275
Size: 1451
Fragments arranged by size:
1451 936 816 706 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2535 3681 4079 4353 4500 4911 2535
Size: 1146 398 274 147 411 2872
Fragments arranged by size:
2872 1146 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5023
Fragments arranged by size:
5023 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2333 1186
Size: 1147 4101
BseYI C'CCAG_C
Cuts at: 2119 2431 4451 4947 2119
Size: 312 2020 496 2420
Fragments arranged by size:
2420 2020 496 312
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2423 1163
Size: 573 687 3988
Fragments arranged by size:
3988 687 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2415 2698 3459 4487 1540
Size: 275 186 414 283 761 1028 2301
Fragments arranged by size:
2301 1028 761 414 283 275 186
BsiHKAI G_wGCw'C
Cuts at: 2325 2352 3309 3394 4465 2325
Size: 27 957 85 1071 3108
Fragments arranged by size:
3108 1071 957 85 27
BsmI GAATG_Cn'
Cuts at: 2413 2759 2852 2413
Size: 346 93 4809
Fragments arranged by size:
4809 346 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2325 2352 2502 2567 3309 3394 4465
Size: 399 535 27 150 65 742 85 1071
Cuts at: 4465 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 535 399 150 85 65
27
BspEI T'CCGG_A
Cuts at: 2535 4911 2535
Size: 2376 2872
BspHI T'CATG_A
Cuts at: 2325 3137 4867 2325
Size: 812 1730 2706
Fragments arranged by size:
2706 1730 812
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2486 2603 844
Size: 1642 117 3489
Fragments arranged by size:
3489 1642 117
BsrBI CCG'CTC
Cuts at: 1394 1823 3135 1394
Size: 429 1312 3507
Fragments arranged by size:
3507 1312 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2293 3743 3917 65
Size: 1183 1045 1450 174 1396
Fragments arranged by size:
1450 1396 1183 1045 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3892 4079 1700
Size: 110 2082 187 2869
Fragments arranged by size:
2869 2082 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5248
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5248
BssSI C'ACGA_G
Cuts at: 3308 4320 3308
Size: 1012 4236
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5248
BstBI TT'CG_AA
Cuts at: 4052 4052
Size: 5248
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5248
BstYI r'GATC_y
Cuts at: 1972 2223 2578 2945 3330 3347 4799 4885 4897
Size: 251 355 367 385 17 1452 86 12
Cuts at: 4897 5243 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 367 355 346 251 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2169 513
Size: 552 939 165 3592
Fragments arranged by size:
3592 939 552 165
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4509
Fragments arranged by size:
4509 683 56
BtsI GCAGTG_nn'
Cuts at: 2764 3558 3578 2764
Size: 794 20 4434
Fragments arranged by size:
4434 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5248
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5213
DraI TTT'AAA
Cuts at: 2552 2552
Size: 5248
DraIII CAC_nnn'GTG
Cuts at: 2199 2199
Size: 5248
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4255 809
Size: 507 2939 1802
Fragments arranged by size:
2939 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2212 2337 2412
Size: 54 1425 50 275 18 382 125 75
Cuts at: 2412 2695 3584 5213 8
Size: 283 889 1629 43
Fragments arranged by size:
1629 1425 889 382 283 275 125 75
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2412 2695 1537
Size: 275 600 283 4090
Fragments arranged by size:
4090 600 283 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2033 3178 1353
Size: 680 1145 3423
Fragments arranged by size:
3423 1145 680
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3835 4209 4355 387
Size: 1064 138 2246 374 146 1280
Fragments arranged by size:
2246 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2323 2350 2323
Size: 27 5221
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3311 3908 4695 4963 1239
Size: 393 1679 597 787 268 1524
Fragments arranged by size:
1679 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2030 2030
Size: 5248
EcoO109I rG'GnC_Cy
Cuts at: 2035 2389 2035
Size: 354 4894
EcoRI G'AATT_C
Cuts at: 2557 2935 2557
Size: 378 4870
FspI TGC'GCA
Cuts at: 2060 3754 2060
Size: 1694 3554
FspAI rTGC'GCAy
Cuts at: 2060 2060
Size: 5248
HaeII r_GCGC'y
Cuts at: 1678 2242 4395 1678
Size: 564 2153 2531
Fragments arranged by size:
2531 2153 564
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2507 2539 3889 3921 3963
Size: 32 135 32 935 32 1350 32 42
Cuts at: 3963 3995 1373
Size: 32 2626
Fragments arranged by size:
2626 1350 935 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2592 2838 669
Size: 988 281 654 246 3079
Fragments arranged by size:
3079 988 654 281 246
HindIII A'AGCT_T
Cuts at: 748 2111 748
Size: 1363 3885
HpaI GTT'AAC
Cuts at: 2838 2838
Size: 5248
KasI G'GCGC_C
Cuts at: 2238 2238
Size: 5248
KpnI G_GTAC'C
Cuts at: 2573 2573
Size: 5248
MfeI C'AATT_G
Cuts at: 2847 2847
Size: 5248
MluI A'CGCG_T
Cuts at: 5124 5124
Size: 5248
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4362 4546 1305
Size: 3057 184 2007
Fragments arranged by size:
3057 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2214 10
Size: 54 1425 343 382 3044
Fragments arranged by size:
3044 1425 382 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2066 2330 3206 3565 3724 518
Size: 648 900 264 876 359 159 2042
Fragments arranged by size:
2042 900 876 648 359 264 159
MspA1I CmG'CkG
Cuts at: 1606 2490 2664 3341 4489 4734 1606
Size: 884 174 677 1148 245 2120
Fragments arranged by size:
2120 1148 884 677 245 174
NarI GG'CG_CC
Cuts at: 2239 2239
Size: 5248
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3757
Fragments arranged by size:
3757 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5248
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5248
NotI GC'GGCC_GC
Cuts at: 2695 2695
Size: 5248
NspI r_CATG'y
Cuts at: 1132 1229 2606 4151 1132
Size: 97 1377 1545 2229
Fragments arranged by size:
2229 1545 1377 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5248
PciI A'CATG_T
Cuts at: 4147 4147
Size: 5248
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5248
PfoI T'CCnGG_A
Cuts at: 2352 2352
Size: 5248
PmeI GTTT'AAAC
Cuts at: 2552 2552
Size: 5248
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3317 3349 4830 4862 3317
Size: 32 1481 32 3703
Fragments arranged by size:
3703 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2389 2389
Size: 5248
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5248
PsiI TTA'TAA
Cuts at: 2818 2818
Size: 5248
PstI C_TGCA'G
Cuts at: 830 2089 2600 830
Size: 1259 511 3478
Fragments arranged by size:
3478 1259 511
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5248
SacI G_AGCT'C
Cuts at: 2325 2352 2325
Size: 27 5221
SalI G'TCGA_C
Cuts at: 1936 2590 1936
Size: 654 4594
SbfI CC_TGCA'GG
Cuts at: 2600 2600
Size: 5248
ScaI AGT'ACT
Cuts at: 3496 5208 3496
Size: 1712 3536
SfcI C'TryA_G
Cuts at: 826 1045 2085 2596 3731 4412 4603 826
Size: 219 1040 511 1135 681 191 1471
Fragments arranged by size:
1471 1135 1040 681 511 219 191
SfoI GGC'GCC
Cuts at: 2240 2240
Size: 5248
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5248
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5248
SmaI CCC'GGG
Cuts at: 1872 2575 1872
Size: 703 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2114 3352 4253 4515 820
Size: 197 926 171 1238 901 262 1553
Fragments arranged by size:
1553 1238 926 901 262 197 171
SnaBI TAC'GTA
Cuts at: 493 3039 493
Size: 2546 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5248
SphI G_CATG'C
Cuts at: 1132 2606 1132
Size: 1474 3774
SspI AAT'ATT
Cuts at: 5 52 3172 5
Size: 47 3120 2081
Fragments arranged by size:
3120 2081 47
StuI AGG'CCT
Cuts at: 1517 2265 1517
Size: 748 4500
StyI C'CwwG_G
Cuts at: 513 1065 2004 2687 513
Size: 552 939 683 3074
Fragments arranged by size:
3074 939 683 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2111 2198 3275 3292 3445 3630 4075
Size: 1255 531 87 1077 17 153 185 445
Cuts at: 4075 325
Size: 1498
Fragments arranged by size:
1498 1255 1077 531 445 185 153 87
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2175 3494 5206
Size: 274 80 33 51 1443 198 1319 1712
Cuts at: 5206 96
Size: 138
Fragments arranged by size:
1712 1443 1319 274 198 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5208 5240 5208
Size: 32 5216
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5248
XbaI T'CTAG_A
Cuts at: 2584 2584
Size: 5248
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5248
XmaI C'CCGG_G
Cuts at: 1870 2573 1870
Size: 703 4545
XmnI GAAnn'nnTTC
Cuts at: 2015 3377 2015
Size: 1362 3886
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3056 276
Size: 53 83 186 936 1522 2468
Fragments arranged by size:
2468 1522 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoRV
FalI FseI NaeI NgoMIV NruI NsiI PacI PmlI
PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI
SexAI SfiI SrfI SwaI XcmI