(Circular) (Six-base) MAPSORT of: pFN21AB5396.seq Check: 7392 from: 1 to: 6604 pFN21AB5396 6604 bp With 182 enzymes: SgfI * February 27, 2009 14:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4414 278 Size: 53 83 186 936 2878 2468 Fragments arranged by size: 2878 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3947 1937 Size: 2010 4594 Acc65I G'GTAC_C Cuts at: 3925 3925 Size: 6604 AclI AA'CG_TT Cuts at: 1563 4731 5104 1563 Size: 3168 373 3063 Fragments arranged by size: 3168 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3800 4667 6051 6319 1632 Size: 2168 867 1384 268 1917 Fragments arranged by size: 2168 1917 1384 867 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6407 AflIII A'CryG_T Cuts at: 1203 2272 5503 6480 1203 Size: 1069 3231 977 1327 Fragments arranged by size: 3231 1327 1069 977 AgeI A'CCGG_T Cuts at: 5435 5435 Size: 6604 AhdI GACnn_n'nnGTC Cuts at: 5333 5333 Size: 6604 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6604 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6604 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3773 3805 3773 Size: 32 6572 AlwNI CAG_nnn'CTG Cuts at: 4347 5919 4347 Size: 1572 5032 ApaLI G'TGCA_C Cuts at: 4661 5817 4661 Size: 1156 5448 ApoI r'AATT_y Cuts at: 1493 2213 2694 2798 3092 3869 3913 4139 4291 Size: 720 481 104 294 777 44 226 152 Cuts at: 4291 6400 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 777 720 481 294 226 152 104 44 AseI AT'TA_AT Cuts at: 160 1032 5158 160 Size: 872 4126 1606 Fragments arranged by size: 4126 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6604 AvaI C'yCGr_G Cuts at: 1870 1943 3687 3929 1870 Size: 73 1744 242 4545 Fragments arranged by size: 4545 1744 242 73 BamHI G'GATC_C Cuts at: 2599 2756 3934 4301 2599 Size: 157 1178 367 4902 Fragments arranged by size: 4902 1178 367 157 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2548 3604 3925 5380 618 Size: 325 217 627 761 1056 321 1455 1842 Fragments arranged by size: 1842 1455 1056 761 627 325 321 217 BanII G_rGCy'C Cuts at: 1391 2233 3923 1391 Size: 842 1690 4072 Fragments arranged by size: 4072 1690 842 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2318 3156 928 Size: 971 419 838 4376 Fragments arranged by size: 4376 971 838 419 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4795 4829 1903 Size: 34 2858 34 3678 Fragments arranged by size: 3678 2858 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2056 3874 4496 5706 2056 Size: 1818 622 1210 2954 Fragments arranged by size: 2954 1818 1210 622 BclI T'GATC_A Cuts at: 1548 1548 Size: 6604 BfrBI ATG'CAT Cuts at: 2630 3134 2630 Size: 504 6100 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5215 136 Size: 107 122 71 631 605 3543 1525 Fragments arranged by size: 3543 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6599 6599 Size: 6604 BlpI GC'TnA_GC Cuts at: 3804 4021 3804 Size: 217 6387 Bme1580I G_kGCm'C Cuts at: 1790 2553 3607 4665 5821 1790 Size: 763 1054 1058 1156 2573 Fragments arranged by size: 2573 1156 1058 1054 763 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6604 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3793 4335 5293 Size: 416 53 83 399 24 2376 542 958 Cuts at: 5293 442 Size: 1753 Fragments arranged by size: 2376 1753 958 542 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6604 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3265 3372 5264 1239 Size: 2026 107 1892 2579 Fragments arranged by size: 2579 2026 1892 107 Bpu10I CC'TnA_GC Cuts at: 3018 3018 Size: 6604 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2417 4729 5594 5892 2417 Size: 2312 865 298 3129 Fragments arranged by size: 3129 2312 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5267 882 Size: 617 3768 2219 Fragments arranged by size: 3768 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4395 493 Size: 711 3191 2702 Fragments arranged by size: 3191 2702 711 BsaBI GATnn'nnATC Cuts at: 6598 6598 Size: 6604 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2923 4411 4793 5428 Size: 53 83 186 936 1390 1488 382 635 Cuts at: 5428 275 Size: 1451 Fragments arranged by size: 1488 1451 1390 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2609 5037 5435 5709 5856 6267 2609 Size: 2428 398 274 147 411 2946 Fragments arranged by size: 2946 2428 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3150 3180 1178 Size: 30 165 30 1747 30 4602 Fragments arranged by size: 4602 1747 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3171 3309 3312 3525 3528 1186 Size: 1985 138 3 213 3 4262 Fragments arranged by size: 4262 1985 213 138 3 3 BseYI C'CCAG_C Cuts at: 5807 6303 5807 Size: 496 6108 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2643 1163 Size: 573 907 5124 Fragments arranged by size: 5124 907 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4054 4815 5843 1540 Size: 275 186 2053 761 1028 2301 Fragments arranged by size: 2301 2053 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2233 3538 3565 4665 4750 5821 2233 Size: 1305 27 1100 85 1071 3016 Fragments arranged by size: 3016 1305 1100 1071 85 27 BsmI GAATG_Cn' Cuts at: 4115 4208 4115 Size: 93 6511 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2233 2553 3538 3565 3607 3923 4665 Size: 399 443 320 985 27 42 316 742 Cuts at: 4665 4750 5821 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 985 742 443 399 320 316 85 42 27 BspEI T'CCGG_A Cuts at: 6267 6267 Size: 6604 BspHI T'CATG_A Cuts at: 4493 6223 4493 Size: 1730 4874 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3959 844 Size: 3115 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4491 1394 Size: 429 2668 3507 Fragments arranged by size: 3507 2668 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3712 5099 5273 65 Size: 1183 2464 1387 174 1396 Fragments arranged by size: 2464 1396 1387 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2925 5248 5435 1700 Size: 110 1115 2323 187 2869 Fragments arranged by size: 2869 2323 1115 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6604 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6604 BssSI C'ACGA_G Cuts at: 4664 5676 4664 Size: 1012 5592 BstAPI GCAn_nnn'nTGC Cuts at: 794 2201 794 Size: 1407 5197 BstBI TT'CG_AA Cuts at: 5408 5408 Size: 6604 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6604 BstYI r'GATC_y Cuts at: 1972 2581 2599 2756 3001 3466 3683 3934 4301 Size: 609 18 157 245 465 217 251 367 Cuts at: 4301 4686 4703 6155 6241 6253 6599 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 609 465 385 367 346 251 245 217 157 86 18 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3670 3679 513 Size: 552 939 1666 9 3438 Fragments arranged by size: 3438 1666 939 552 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3784 487 Size: 683 56 2558 3307 Fragments arranged by size: 3307 2558 683 56 BtsI GCAGTG_nn' Cuts at: 3288 4120 4914 4934 3288 Size: 832 794 20 4958 Fragments arranged by size: 4958 832 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6604 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6569 DraI TTT'AAA Cuts at: 3908 3908 Size: 6604 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5611 809 Size: 507 4295 1802 Fragments arranged by size: 4295 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4051 4940 6569 Size: 54 1425 50 275 18 2221 889 1629 Cuts at: 6569 8 Size: 43 Fragments arranged by size: 2221 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4051 1537 Size: 275 2239 4090 Fragments arranged by size: 4090 2239 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2040 3502 4534 1353 Size: 687 1462 1032 3423 Fragments arranged by size: 3423 1462 1032 687 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5191 5565 5711 387 Size: 1064 138 3602 374 146 1280 Fragments arranged by size: 3602 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2231 2231 Size: 6604 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3265 3372 3800 4667 5264 6051 6319 Size: 393 1633 107 428 867 597 787 268 Cuts at: 6319 1239 Size: 1524 Fragments arranged by size: 1633 1524 867 787 597 428 393 268 107 EcoNI CCTnn'n_nnAGG Cuts at: 3573 3573 Size: 6604 EcoO109I rG'GnC_Cy Cuts at: 2366 3647 2366 Size: 1281 5323 EcoRI G'AATT_C Cuts at: 2798 3913 4291 2798 Size: 1115 378 5111 Fragments arranged by size: 5111 1115 378 FspI TGC'GCA Cuts at: 5110 5110 Size: 6604 HaeII r_GCGC'y Cuts at: 1678 3262 5751 1678 Size: 1584 2489 2531 Fragments arranged by size: 2531 2489 1584 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2591 2623 2888 2920 5245 Size: 32 135 32 1019 32 265 32 2325 Cuts at: 5245 5277 5319 5351 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2325 1019 265 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3948 4194 669 Size: 988 281 2010 246 3079 Fragments arranged by size: 3079 2010 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6604 HpaI GTT'AAC Cuts at: 4194 4194 Size: 6604 KpnI G_GTAC'C Cuts at: 3929 3929 Size: 6604 MfeI C'AATT_G Cuts at: 2196 4203 2196 Size: 2007 4597 MluI A'CGCG_T Cuts at: 6480 6480 Size: 6604 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2734 5718 5902 1305 Size: 1429 2984 184 2007 Fragments arranged by size: 2984 2007 1429 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4782 Fragments arranged by size: 4782 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2296 2729 4562 4921 5080 518 Size: 648 1130 433 1833 359 159 2042 Fragments arranged by size: 2042 1833 1130 648 433 359 159 MspA1I CmG'CkG Cuts at: 1606 3164 4020 4697 5845 6090 1606 Size: 1558 856 677 1148 245 2120 Fragments arranged by size: 2120 1558 1148 856 677 245 NaeI GCC'GGC Cuts at: 2927 2927 Size: 6604 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5113 Fragments arranged by size: 5113 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6604 NgoMIV G'CCGG_C Cuts at: 2925 2925 Size: 6604 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6604 NotI GC'GGCC_GC Cuts at: 4051 4051 Size: 6604 NsiI A_TGCA'T Cuts at: 2632 3136 2632 Size: 504 6100 NspI r_CATG'y Cuts at: 1132 1229 2276 3331 3962 5507 1132 Size: 97 1047 1055 631 1545 2229 Fragments arranged by size: 2229 1545 1055 1047 631 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6604 PciI A'CATG_T Cuts at: 2272 5503 2272 Size: 3231 3373 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6604 PmeI GTTT'AAAC Cuts at: 3908 3908 Size: 6604 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4673 4705 6186 6218 4673 Size: 32 1481 32 5059 Fragments arranged by size: 5059 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2366 3647 2366 Size: 1281 5323 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6604 PsiI TTA'TAA Cuts at: 4174 4174 Size: 6604 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2395 2427 2395 Size: 32 6572 PstI C_TGCA'G Cuts at: 830 3956 830 Size: 3126 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6604 PvuII CAG'CTG Cuts at: 3164 3164 Size: 6604 SacI G_AGCT'C Cuts at: 2233 2233 Size: 6604 SalI G'TCGA_C Cuts at: 1936 3946 1936 Size: 2010 4594 SbfI CC_TGCA'GG Cuts at: 3956 3956 Size: 6604 ScaI AGT'ACT Cuts at: 4852 6564 4852 Size: 1712 4892 SfcI C'TryA_G Cuts at: 826 1045 2243 2317 3952 5087 5768 5959 826 Size: 219 1198 74 1635 1135 681 191 1471 Fragments arranged by size: 1635 1471 1198 1135 681 219 191 74 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6604 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6604 SmaI CCC'GGG Cuts at: 1872 3931 1872 Size: 2059 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2396 4708 5609 5871 820 Size: 197 926 453 2312 901 262 1553 Fragments arranged by size: 2312 1553 926 901 453 262 197 SnaBI TAC'GTA Cuts at: 493 4395 493 Size: 3902 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6604 SphI G_CATG'C Cuts at: 1132 3331 3962 1132 Size: 2199 631 3774 Fragments arranged by size: 3774 2199 631 SspI AAT'ATT Cuts at: 5 52 2151 3072 4528 5 Size: 47 2099 921 1456 2081 Fragments arranged by size: 2099 2081 1456 921 47 StuI AGG'CCT Cuts at: 1517 2115 2390 3278 1517 Size: 598 275 888 4843 Fragments arranged by size: 4843 888 598 275 StyI C'CwwG_G Cuts at: 513 1065 2004 2522 2713 3548 4043 513 Size: 552 939 518 191 835 495 3074 Fragments arranged by size: 3074 939 835 552 518 495 191 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4631 4648 4801 4986 5431 325 Size: 1255 3051 17 153 185 445 1498 Fragments arranged by size: 3051 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2239 2469 4850 Size: 274 80 33 51 1443 262 230 2381 Cuts at: 4850 6562 96 Size: 1712 138 Fragments arranged by size: 2381 1712 1443 274 262 230 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2431 2463 6564 6596 2431 Size: 32 4101 32 2439 Fragments arranged by size: 4101 2439 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6604 XbaI T'CTAG_A Cuts at: 3940 3940 Size: 6604 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6604 XmaI C'CCGG_G Cuts at: 1870 3929 1870 Size: 2059 4545 XmnI GAAnn'nnTTC Cuts at: 2151 4733 2151 Size: 2582 4022 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4412 276 Size: 53 83 186 936 2878 2468 Fragments arranged by size: 2878 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NarI NruI PacI PfoI PmlI PspOMI PspXI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI