Restriction map for
pFN21AB5383
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# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI Alw44I AocI
ApaI ApaLI AscI Asp718I AspEI
AspI AviII AvrII BalI BamHI
BbrPI BcgI BciVI BclI BfrI
BglI BlnI BsaAI BsaBI BsaI
BsaWI BseAI BsgI BsiWI BsmBI
Bsp1407I BspDI BspEI BspMI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I ClaI
CpoI Eam1105I Eco81I EcoO65I EcoRV
EcoT22I FbaI FseI FspI HgaI
HincII HindII HpaI KpnI MaeII
MamI MluI MluNI MroI MscI
MslI NcoI NdeI NheI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PvuI PvuII
RsrII SacI SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
StuI Tth111I Van91I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 63 enzymes) #
###################################
AcyI 64 GGCGCC G[GA]^CG[CT]C
AfaI 359 GTAC GT^AC
AflIII 449 ACATGT A^C[GA][CT]GT
AlwI 96 GATCC GGATC(4/5)
AlwNI 1528 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1205 AGCGCT AGC^GCT
AseI 1477 ATTAAT AT^TAAT
AsnI 1477 ATTAAT AT^TAAT
Asp700I 1570 GAAACTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 4 CCCGAG C^[CT]CG[GA]G
BanII 1256 GGGCTC G[GA]GC[CT]^C
BbeI 64 GGCGCC GGCGC^C
BbsI 991 GAAGAC GAAGAC(2/6)
BbvCI 1084 GCTGAGG CCTCAGC(-5/-2)
BfaI 1393 CTAG C^TAG
BglII 609 AGATCT A^GATCT
BlpI 1201 GCTGAGC GC^T[ACGT]AGC
BpmI 1280 CTCCAG CTGGAG(16/14)
Bpu1102I 1201 GCTGAGC GC^T[ACGT]AGC
BpuAI 991 GAAGAC GAAGAC(2/6)
BsaHI 64 GGCGCC G[GA]^CG[CT]C
BseRI 1087 GAGGAG GAGGAG(10/8)
BsiEI 51 CGGCCG CG[GA][CT]^CG
BsmAI 453 GTCTC GTCTC(1/5)
BsmFI 646 GGGAC GGGAC(10/14)
BsoBI 4 CCCGAG C^[CT]CG[GA]G
BspHI 1215 TCATGA T^CATGA
BspLU11I 449 ACATGT A^CATGT
BsrBI 24 GAGCGG CCGCTC(-3/-3)
BsrDI 333 CATTGC GCAATG(2/0)
CelII 1201 GCTGAGC GC^T[ACGT]AGC
DraII 416 AGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 1325 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 444 GACTGACATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 54 CCGCGG C^C[GA][CT]GG
EaeI 51 CGGCCG [CT]^GGCC[GA]
EagI 51 CGGCCG C^GGCCG
EclXI 51 CGGCCG C^GGCCG
Eco47III 1205 AGCGCT AGC^GCT
Eco52I 51 CGGCCG C^GGCCG
EcoNI 420 CCTGGAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 416 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRI 1000 GAATTC G^AATTC
EcoT14I 153 CCTTGG C^C[AT][AT]GG
Hin1I 64 GGCGCC G[GA]^CG[CT]C
KasI 64 GGCGCC G^GCGCC
KspI 54 CCGCGG CCGC^GG
MaeI 1393 CTAG C^TAG
MspA1I 54 CCGCGG C[AC]G^C[GT]G
NarI 64 GGCGCC GG^CGCC
NotI 50 GCGGCCGC GC^GGCCGC
PshBI 1477 ATTAAT AT^TAAT
PstI 1469 CTGCAG CTGCA^G
RcaI 1215 TCATGA T^CATGA
RsaI 359 GTAC GT^AC
SacII 54 CCGCGG CCGC^GG
SfoI 64 GGCGCC GGC^GCC
SphI 1449 GCATGC GCATG^C
SspI 1313 AATATT AAT^ATT
StyI 153 CCTTGG C^C[AT][AT]GG
SwaI 1097 ATTTAAAT ATTT^AAAT
Tsp45I 473 GTGAC ^GT[GC]AC
XmnI 1570 GAAACTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 55 CGCG CG^CG
79 CGCG
AspHI 1277 GTGCTC G[AT]GC[AT]^C
1550 GTGCTC
BcnI 20 CCCGG CC^[GC]GG
38 CCGGG
BsiHKAI 1277 GTGCTC G[AT]GC[AT]^C
1550 GTGCTC
BsmI 266 GAATGC GAATGC(1/-1)
1583 GCATTC
BstUI 55 CGCG CG^CG
79 CGCG
BstXI 263 CCAGAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1502 CCAACATTTTGG
BstYI 95 AGATCC [GA]^GATC[CT]
609 AGATCT
CfoI 65 GCGC GCG^C
1206 GCGC
DraI 849 TTTAAA TTT^AAA
1098 TTTAAA
EarI 832 GAAGAG CTCTTC(1/4)
1251 GAAGAG
FauI 26 GCGGG CCCGC(4/6)
56 GCGGG
HaeII 64 GGCGCC [GA]GCGC^[CT]
1205 AGCGCT
HhaI 65 GCGC GCG^C
1206 GCGC
HinP1I 65 GCGC G^CGC
1206 GCGC
HindIII 364 AAGCTT A^AGCTT
637 AAGCTT
Ksp632I 832 GAAGAG CTCTTC(1/4)
1251 GAAGAG
MflI 95 AGATCC [GA]^GATC[CT]
609 AGATCT
MvnI 55 CGCG CG^CG
79 CGCG
NaeI 46 GCCGGC GCC^GGC
1405 GCCGGC
NciI 20 CCCGG CC^[GC]GG
38 CCGGG
NgoMIV 46 GCCGGC G^CCGGC
1405 GCCGGC
PleI 1557 GACTC GAGTC(4/5)
403 GAGTC
SfcI 1424 CTACAG C^T[GA][CT]AG
1469 CTGCAG
SmlI 290 CTCAAG C^T[CT][GA]AG
1120 CTCAAG
TaqI 456 TCGA T^CGA
1614 TCGA
TthHB8I 456 TCGA T^CGA
1614 TCGA
XcmI 263 CCAGAATGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
767 CCAAAACAGACATGG
XhoII 95 AGATCC [GA]^GATC[CT]
609 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AcsI AluI ApoI AvaII
BanI BbvI BmyI BsaJI BsiYI
BslI Bsp1286I BsrFI BsrI BssKI
BstF5I BstNI Cac8I Cfr10I Cfr13I
DdeI DpnII EcoRII Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI MaeIII MboI MboII MfeI
MnlI MseI MspI MunI MvaI
MwoI NdeII NlaIII NlaIV NspI
Sau3AI Sau96I ScrFI SfaNI TfiI
Tru9I TseI Tsp509I TspRI