Restriction map for
pFN21AB5341
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# NO CUT ENZYMES ( 155 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AviII AvrII BalI
BamHI BanI BbeI BbrPI BbvCI
BcgI BclI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstXI BstZ17I
CelII ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeII
HgaI Hin1I HindIII HpaI HphI
KasI KpnI KspI MaeII MamI
MfeI MluI MluNI MroI MscI
MslI MunI MvnI NaeI NarI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AlwNI 876 CAGGTACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 862 CCTGAGG CC^T[ACGT]AGG
ApaI 571 GGGCCC GGGCC^C
AspI 868 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 110 CTCGGG C^[CT]CG[GA]G
AvaII 963 GGACC G^G[AT]CC
BbsI 634 GTCTTC GAAGAC(2/6)
BciVI 487 GGATAC GTATCC(6/5)
BcnI 226 CCCGG CC^[GC]GG
BfaI 505 CTAG C^TAG
BglII 431 AGATCT A^GATCT
BpuAI 634 GTCTTC GAAGAC(2/6)
BsoBI 110 CTCGGG C^[CT]CG[GA]G
BspMI 854 ACCTGC ACCTGC(4/8)
BsrFI 59 ACCGGC [GA]^CCGG[CT]
BstAPI 685 GCAAAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 862 CCTGAGG CC^T[ACGT]AGG
CfoI 702 GCGC GCG^C
Cfr10I 59 ACCGGC [GA]^CCGG[CT]
EaeI 261 TGGCCG [CT]^GGCC[GA]
EarI 761 GAAGAG CTCTTC(1/4)
Eco81I 862 CCTGAGG CC^T[ACGT]AGG
HhaI 702 GCGC GCG^C
HinP1I 702 GCGC G^CGC
HincII 424 GTTGAC GT[CT]^[GA]AC
HindII 424 GTTGAC GT[CT]^[GA]AC
Ksp632I 761 GAAGAG CTCTTC(1/4)
MaeI 505 CTAG C^TAG
MspA1I 958 CAGCGG C[AC]G^C[GT]G
NciI 226 CCCGG CC^[GC]GG
NdeI 782 CATATG CA^TATG
PflFI 868 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 962 GGGACCC [GA]G^G[AT]CC[CT]
Tsp45I 437 GTCAC ^GT[GC]AC
Tth111I 868 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AluI 231 AGCT AG^CT
294 AGCT
BsmAI 607 GAGAC GTCTC(1/5)
954 GTCTC
BsmFI 78 GGGAC GGGAC(10/14)
962 GGGAC
BstF5I 318 GGATG GGATG(2/0)
408 GGATG
BstNI 404 CCAGG CC^[AT]GG
657 CCTGG
DraII 947 AGGCCCT [GA]G^G[ACGT]CC[CT]
962 GGGACCC
DsaI 311 CCATGG C^C[GA][CT]GG
483 CCATGG
EcoO109I 947 AGGCCCT [GA]G^G[ACGT]CC[CT]
962 GGGACCC
EcoRII 404 CCAGG ^CC[AT]GG
657 CCTGG
FauI 102 GCGGG CCCGC(4/6)
960 GCGGG
FokI 318 GGATG GGATG(9/13)
408 GGATG
HapII 60 CCGG C^CGG
227 CCGG
HpaII 60 CCGG C^CGG
227 CCGG
MaeIII 211 GTAAC ^GT[ACGT]AC
437 GTCAC
MspI 60 CCGG C^CGG
227 CCGG
MvaI 404 CCAGG CC^[AT]GG
657 CCTGG
NcoI 311 CCATGG C^CATGG
483 CCATGG
PleI 832 GACTC GAGTC(4/5)
358 GAGTC
SacI 230 GAGCTC GAGCT^C
293 GAGCTC
SfaNI 379 GATGC GCATC(5/9)
449 GCATC
TaqI 377 TCGA T^CGA
992 TCGA
TthHB8I 377 TCGA T^CGA
992 TCGA
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# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AfaI AlwI AspHI BanII
BbvI BmyI BpmI BsaJI BseRI
BsiHKAI BsiYI BslI Bsp1286I BsrI
BssKI BstYI Cac8I Cfr13I DdeI
DpnII EcoT14I Fnu4HI HaeIII HinfI
ItaI MboI MboII MflI MnlI
MseI MwoI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI StyI
TfiI Tru9I TseI Tsp509I TspRI
XhoII