(Circular) (Six-base) MAPSORT of: pFN21AB5268.seq Check: 3988 from: 1 to: 5809 pFN21AB5268 5809 bp With 182 enzymes: SgfI * February 27, 2009 14:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3619 278 Size: 53 83 186 936 2083 2468 Fragments arranged by size: 2468 2083 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3152 1937 Size: 1215 4594 Acc65I G'GTAC_C Cuts at: 3130 3130 Size: 5809 AclI AA'CG_TT Cuts at: 1563 3936 4309 1563 Size: 2373 373 3063 Fragments arranged by size: 3063 2373 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3872 5256 5524 1632 Size: 2240 1384 268 1917 Fragments arranged by size: 2240 1917 1384 268 AfeI AGC'GCT Cuts at: 2736 2736 Size: 5809 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5612 AflIII A'CryG_T Cuts at: 1203 4708 5685 1203 Size: 3505 977 1327 Fragments arranged by size: 3505 1327 977 AgeI A'CCGG_T Cuts at: 4640 4640 Size: 5809 AhdI GACnn_n'nnGTC Cuts at: 4538 4538 Size: 5809 AleI CACnn'nnGTG Cuts at: 1166 2408 1166 Size: 1242 4567 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5809 AlwNI CAG_nnn'CTG Cuts at: 3552 5124 3552 Size: 1572 4237 ApaI G_GGCC'C Cuts at: 2258 2517 2536 3014 2258 Size: 259 19 478 5053 Fragments arranged by size: 5053 478 259 19 ApaLI G'TGCA_C Cuts at: 2357 2925 3866 5022 2357 Size: 568 941 1156 3144 Fragments arranged by size: 3144 1156 941 568 ApoI r'AATT_y Cuts at: 1493 3118 3344 3496 5605 1493 Size: 1625 226 152 2109 1697 Fragments arranged by size: 2109 1697 1625 226 152 AseI AT'TA_AT Cuts at: 160 1032 4363 160 Size: 872 3331 1606 Fragments arranged by size: 3331 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5809 AvaI C'yCGr_G Cuts at: 1870 1943 2231 2315 2510 2708 3050 3134 1870 Size: 73 288 84 195 198 342 84 4545 Fragments arranged by size: 4545 342 288 198 195 84 84 73 BamHI G'GATC_C Cuts at: 3139 3506 3139 Size: 367 5442 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2573 2791 2967 3130 4585 Size: 325 217 627 786 218 176 163 1455 Cuts at: 4585 618 Size: 1842 Fragments arranged by size: 1842 1455 786 627 325 218 217 176 163 BanII G_rGCy'C Cuts at: 1391 2258 2517 2536 3014 3074 3128 1391 Size: 867 259 19 478 60 54 4072 Fragments arranged by size: 4072 867 478 259 60 54 19 BbeI G_GCGC'C Cuts at: 2577 2795 2577 Size: 218 5591 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4838 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4000 4034 1903 Size: 34 2063 34 3678 Fragments arranged by size: 3678 2063 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3701 4911 3701 Size: 1210 4599 BclI T'GATC_A Cuts at: 1548 1548 Size: 5809 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2233 2414 2521 Size: 107 122 71 631 605 561 181 107 Cuts at: 2521 2540 2626 2695 2822 2846 2855 4420 136 Size: 19 86 69 127 24 9 1565 1525 Fragments arranged by size: 1565 1525 631 605 561 181 127 122 107 107 86 71 69 24 19 9 BglII A'GATC_T Cuts at: 5804 5804 Size: 5809 BlpI GC'TnA_GC Cuts at: 3226 3226 Size: 5809 Bme1580I G_kGCm'C Cuts at: 1790 2089 2258 2361 2517 2536 2929 2980 3014 Size: 299 169 103 156 19 393 51 34 Cuts at: 3014 3870 5026 1790 Size: 856 1156 2573 Fragments arranged by size: 2573 1156 856 393 299 169 156 103 51 34 19 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5809 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2915 3540 4498 Size: 416 53 83 399 24 1498 625 958 Cuts at: 4498 442 Size: 1753 Fragments arranged by size: 1753 1498 958 625 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5809 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2978 3021 4469 1239 Size: 1739 43 1448 2579 Fragments arranged by size: 2579 1739 1448 43 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3934 4799 5097 3934 Size: 865 298 4646 Fragments arranged by size: 4646 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2992 4472 882 Size: 617 1493 1480 2219 Fragments arranged by size: 2219 1493 1480 617 BsaAI yAC'GTr Cuts at: 493 1204 3600 493 Size: 711 2396 2702 Fragments arranged by size: 2702 2396 711 BsaBI GATnn'nnATC Cuts at: 5803 5803 Size: 5809 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2574 2792 3616 3998 Size: 53 83 186 936 1041 218 824 382 Cuts at: 3998 4633 275 Size: 635 1451 Fragments arranged by size: 1451 1041 936 824 635 382 218 186 83 53 BsaWI w'CCGG_w Cuts at: 4242 4640 4914 5061 5472 4242 Size: 398 274 147 411 4579 Fragments arranged by size: 4579 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5584 Fragments arranged by size: 5584 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5809 BseYI C'CCAG_C Cuts at: 2092 2206 2236 2431 2501 2731 5012 5508 2092 Size: 114 30 195 70 230 2281 496 2393 Fragments arranged by size: 2393 2281 496 230 195 114 70 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2293 2935 1163 Size: 573 557 642 4037 Fragments arranged by size: 4037 642 573 557 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2190 2384 2525 2630 3259 4020 Size: 275 186 189 194 141 105 629 761 Cuts at: 4020 5048 1540 Size: 1028 2301 Fragments arranged by size: 2301 1028 761 629 275 194 189 186 141 105 BsiHKAI G_wGCw'C Cuts at: 2361 2397 2403 2929 3870 3955 5026 2361 Size: 36 6 526 941 85 1071 3144 Fragments arranged by size: 3144 1071 941 526 85 36 6 BsmI GAATG_Cn' Cuts at: 3320 3413 3320 Size: 93 5716 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2089 2258 2361 2397 2403 2517 2536 Size: 399 299 169 103 36 6 114 19 Cuts at: 2536 2929 2980 3014 3074 3128 3870 3955 5026 Size: 393 51 34 60 54 742 85 1071 Cuts at: 5026 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 393 299 169 114 103 85 60 54 51 36 34 19 6 BspEI T'CCGG_A Cuts at: 5472 5472 Size: 5809 BspHI T'CATG_A Cuts at: 3698 5428 3698 Size: 1730 4079 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2187 3164 844 Size: 1343 977 3489 Fragments arranged by size: 3489 1343 977 BsrBI CCG'CTC Cuts at: 1394 1823 2276 2294 2527 2652 2692 3696 1394 Size: 429 453 18 233 125 40 1004 3507 Fragments arranged by size: 3507 1004 453 429 233 125 40 18 BsrDI GCAATG_nn' Cuts at: 65 1248 2993 4304 4478 65 Size: 1183 1745 1311 174 1396 Fragments arranged by size: 1745 1396 1311 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2302 2629 4453 4640 1700 Size: 110 492 327 1824 187 2869 Fragments arranged by size: 2869 1824 492 327 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5809 BssHII G'CGCG_C Cuts at: 1925 2103 2436 2633 1925 Size: 178 333 197 5101 Fragments arranged by size: 5101 333 197 178 BssSI C'ACGA_G Cuts at: 2396 3869 4881 2396 Size: 1473 1012 3324 Fragments arranged by size: 3324 1473 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2365 794 Size: 1571 4238 BstBI TT'CG_AA Cuts at: 4613 4613 Size: 5809 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5809 BstYI r'GATC_y Cuts at: 1972 3139 3506 3891 3908 5360 5446 5458 5804 Size: 1167 367 385 17 1452 86 12 346 Cuts at: 5804 1972 Size: 1977 Fragments arranged by size: 1977 1452 1167 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2615 2615 Size: 5809 BtgI C'CryG_G Cuts at: 513 1065 2004 2418 2624 2742 2808 2972 513 Size: 552 939 414 206 118 66 164 3350 Fragments arranged by size: 3350 939 552 414 206 164 118 66 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5070 Fragments arranged by size: 5070 683 56 BtsI GCAGTG_nn' Cuts at: 2087 2927 3325 4119 4139 2087 Size: 840 398 794 20 3757 Fragments arranged by size: 3757 840 794 398 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5809 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5774 DraI TTT'AAA Cuts at: 3113 3113 Size: 5809 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4816 809 Size: 507 3500 1802 Fragments arranged by size: 3500 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2187 2217 Size: 54 1425 50 275 18 177 180 30 Cuts at: 2217 2322 2369 2381 2522 2627 2739 3256 4145 Size: 105 47 12 141 105 112 517 889 Cuts at: 4145 5774 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 517 275 180 177 141 112 105 105 54 50 47 43 30 18 12 EagI C'GGCC_G Cuts at: 1537 1812 2187 2381 2522 2627 3256 1537 Size: 275 375 194 141 105 629 4090 Fragments arranged by size: 4090 629 375 275 194 141 105 EarI CTCTTCn'nnn_ Cuts at: 1353 3739 1353 Size: 2386 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2278 3082 4396 4770 4916 387 Size: 1064 138 689 804 1314 374 146 1280 Fragments arranged by size: 1314 1280 1064 804 689 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2978 3021 3872 4469 5256 5524 1239 Size: 393 1346 43 851 597 787 268 1524 Fragments arranged by size: 1524 1346 851 787 597 393 268 43 EcoNI CCTnn'n_nnAGG Cuts at: 3018 3018 Size: 5809 EcoO109I rG'GnC_Cy Cuts at: 2255 2506 2514 2533 2619 2640 3010 3011 3023 Size: 251 8 19 86 21 370 1 12 Cuts at: 3023 2255 Size: 5041 Fragments arranged by size: 5041 370 251 86 21 19 12 8 1 EcoRI G'AATT_C Cuts at: 3118 3496 3118 Size: 378 5431 FspI TGC'GCA Cuts at: 2126 4315 2126 Size: 2189 3620 HaeII r_GCGC'y Cuts at: 1678 2577 2738 2795 2890 4956 1678 Size: 899 161 57 95 2066 2531 Fragments arranged by size: 2531 2066 899 161 95 57 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4450 4482 4524 4556 1373 Size: 32 135 32 2878 32 42 32 2626 Fragments arranged by size: 2878 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3153 3399 669 Size: 988 281 1215 246 3079 Fragments arranged by size: 3079 1215 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5809 HpaI GTT'AAC Cuts at: 3399 3399 Size: 5809 KasI G'GCGC_C Cuts at: 2573 2791 2573 Size: 218 5591 KpnI G_GTAC'C Cuts at: 3134 3134 Size: 5809 MfeI C'AATT_G Cuts at: 3408 3408 Size: 5809 MluI A'CGCG_T Cuts at: 5685 5685 Size: 5809 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4923 5107 1305 Size: 3618 184 2007 Fragments arranged by size: 3618 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 10 Size: 54 1425 343 177 3810 Fragments arranged by size: 3810 1425 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 2408 2591 3767 4126 4285 518 Size: 648 1242 183 1176 359 159 2042 Fragments arranged by size: 2042 1242 1176 648 359 183 159 MspA1I CmG'CkG Cuts at: 1606 2069 2206 2365 2420 2810 2974 3225 3902 Size: 463 137 159 55 390 164 251 677 Cuts at: 3902 5050 5295 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 463 390 251 245 164 159 137 55 NaeI GCC'GGC Cuts at: 2304 2631 2304 Size: 327 5482 NarI GG'CG_CC Cuts at: 2574 2792 2574 Size: 218 5591 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4318 Fragments arranged by size: 4318 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5809 NgoMIV G'CCGG_C Cuts at: 2302 2629 2302 Size: 327 5482 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5809 NotI GC'GGCC_GC Cuts at: 2187 2381 3256 2187 Size: 194 875 4740 Fragments arranged by size: 4740 875 194 NspI r_CATG'y Cuts at: 1132 1229 3167 4712 1132 Size: 97 1938 1545 2229 Fragments arranged by size: 2229 1938 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5809 PciI A'CATG_T Cuts at: 4708 4708 Size: 5809 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5809 PmeI GTTT'AAAC Cuts at: 3113 3113 Size: 5809 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3878 3910 5391 5423 3878 Size: 32 1481 32 4264 Fragments arranged by size: 4264 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2640 2640 Size: 5809 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5809 PsiI TTA'TAA Cuts at: 3379 3379 Size: 5809 PspOMI G'GGCC_C Cuts at: 2254 2513 2532 3010 2254 Size: 259 19 478 5053 Fragments arranged by size: 5053 478 259 19 PstI C_TGCA'G Cuts at: 830 2886 3161 830 Size: 2056 275 3478 Fragments arranged by size: 3478 2056 275 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5809 PvuII CAG'CTG Cuts at: 2069 2069 Size: 5809 SacII CC_GC'GG Cuts at: 2421 2811 2975 2421 Size: 390 164 5255 Fragments arranged by size: 5255 390 164 SalI G'TCGA_C Cuts at: 1936 3151 1936 Size: 1215 4594 SanDI GG'GwC_CC Cuts at: 2640 2640 Size: 5809 SbfI CC_TGCA'GG Cuts at: 3161 3161 Size: 5809 ScaI AGT'ACT Cuts at: 4057 5769 4057 Size: 1712 4097 SfcI C'TryA_G Cuts at: 826 1045 2882 3157 4292 4973 5164 826 Size: 219 1837 275 1135 681 191 1471 Fragments arranged by size: 1837 1471 1135 681 275 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2521 2540 2626 2521 Size: 19 86 5704 Fragments arranged by size: 5704 86 19 SfoI GGC'GCC Cuts at: 2575 2793 2575 Size: 218 5591 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5809 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5809 SmaI CCC'GGG Cuts at: 1872 2233 2710 3136 1872 Size: 361 477 426 4545 Fragments arranged by size: 4545 477 426 361 SmlI C'TyrA_G Cuts at: 820 1017 1943 3913 4814 5076 820 Size: 197 926 1970 901 262 1553 Fragments arranged by size: 1970 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3600 493 Size: 3107 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5809 SphI G_CATG'C Cuts at: 1132 3167 1132 Size: 2035 3774 SrfI GCCC'GGGC Cuts at: 2233 2233 Size: 5809 SspI AAT'ATT Cuts at: 5 52 3733 5 Size: 47 3681 2081 Fragments arranged by size: 3681 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5809 StyI C'CwwG_G Cuts at: 513 1065 2004 3248 513 Size: 552 939 1244 3074 Fragments arranged by size: 3074 1244 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2482 3836 3853 4006 4191 4636 325 Size: 1255 902 1354 17 153 185 445 1498 Fragments arranged by size: 1498 1354 1255 902 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4055 5767 96 Size: 274 80 33 51 1443 2078 1712 138 Fragments arranged by size: 2078 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5769 5801 5769 Size: 32 5777 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5809 XbaI T'CTAG_A Cuts at: 3145 3145 Size: 5809 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2203 2203 Size: 5809 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5809 XmaI C'CCGG_G Cuts at: 1870 2231 2708 3134 1870 Size: 361 477 426 4545 Fragments arranged by size: 4545 477 426 361 XmnI GAAnn'nnTTC Cuts at: 3938 3938 Size: 5809 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3617 276 Size: 53 83 186 936 2083 2468 Fragments arranged by size: 2468 2083 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SapI SexAI SwaI