(Circular) (Six-base) MAPSORT of: pFN21AB5258.seq Check: 1552 from: 1 to: 5677 pFN21AB5258 5677 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3487 278 Size: 53 83 186 936 1951 2468 Fragments arranged by size: 2468 1951 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2617 2695 3020 1937 Size: 680 78 325 4594 Fragments arranged by size: 4594 680 325 78 Acc65I G'GTAC_C Cuts at: 2844 2998 2844 Size: 154 5523 AclI AA'CG_TT Cuts at: 1563 3804 4177 1563 Size: 2241 373 3063 Fragments arranged by size: 3063 2241 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3740 5124 5392 1632 Size: 2108 1384 268 1917 Fragments arranged by size: 2108 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5480 AflIII A'CryG_T Cuts at: 1203 4576 5553 1203 Size: 3373 977 1327 Fragments arranged by size: 3373 1327 977 AgeI A'CCGG_T Cuts at: 4508 4508 Size: 5677 AhdI GACnn_n'nnGTC Cuts at: 2820 4406 2820 Size: 1586 4091 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5677 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5677 AlwNI CAG_nnn'CTG Cuts at: 2387 3420 4992 2387 Size: 1033 1572 3072 Fragments arranged by size: 3072 1572 1033 ApaI G_GGCC'C Cuts at: 2750 2750 Size: 5677 ApaLI G'TGCA_C Cuts at: 3734 4890 3734 Size: 1156 4521 ApoI r'AATT_y Cuts at: 1493 2938 2986 3212 3364 5473 1493 Size: 1445 48 226 152 2109 1697 Fragments arranged by size: 2109 1697 1445 226 152 48 AseI AT'TA_AT Cuts at: 160 1032 4231 160 Size: 872 3199 1606 Fragments arranged by size: 3199 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5677 AvaI C'yCGr_G Cuts at: 1870 1943 2119 2951 3002 1870 Size: 73 176 832 51 4545 Fragments arranged by size: 4545 832 176 73 51 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2828 2861 2828 Size: 33 5644 BamHI G'GATC_C Cuts at: 3007 3374 3007 Size: 367 5310 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2140 2844 2998 4453 618 Size: 325 217 627 353 704 154 1455 1842 Fragments arranged by size: 1842 1455 704 627 353 325 217 154 BanII G_rGCy'C Cuts at: 1391 2750 2996 1391 Size: 1359 246 4072 Fragments arranged by size: 4072 1359 246 BbeI G_GCGC'C Cuts at: 2144 2144 Size: 5677 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4706 BbvCI CC'TCA_GC Cuts at: 2063 2973 2063 Size: 910 4767 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3868 3902 1903 Size: 34 1931 34 3678 Fragments arranged by size: 3678 1931 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3569 4779 3569 Size: 1210 4467 BclI T'GATC_A Cuts at: 1548 1548 Size: 5677 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2124 4288 136 Size: 107 122 71 631 605 452 2164 1525 Fragments arranged by size: 2164 1525 631 605 452 122 107 71 BglII A'GATC_T Cuts at: 5672 5672 Size: 5677 BlpI GC'TnA_GC Cuts at: 2958 3094 2958 Size: 136 5541 Bme1580I G_kGCm'C Cuts at: 1790 2750 3738 4894 1790 Size: 960 988 1156 2573 Fragments arranged by size: 2573 1156 988 960 BmgBI CAC'GTC Cuts at: 1318 2453 1318 Size: 1135 4542 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3408 4366 442 Size: 416 53 83 399 24 1991 958 1753 Fragments arranged by size: 1991 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5677 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4337 1239 Size: 3098 2579 Bpu10I CC'TnA_GC Cuts at: 2063 2973 2063 Size: 910 4767 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3802 4667 4965 3802 Size: 865 298 4514 Fragments arranged by size: 4514 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2015 2596 2807 4340 882 Size: 617 516 581 211 1533 2219 Fragments arranged by size: 2219 1533 617 581 516 211 BsaAI yAC'GTr Cuts at: 493 1204 3468 493 Size: 711 2264 2702 Fragments arranged by size: 2702 2264 711 BsaBI GATnn'nnATC Cuts at: 5671 5671 Size: 5677 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2141 2614 2875 3484 Size: 53 83 186 936 608 473 261 609 Cuts at: 3484 3866 4501 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 635 609 608 473 382 261 186 83 53 BsaWI w'CCGG_w Cuts at: 4110 4508 4782 4929 5340 4110 Size: 398 274 147 411 4447 Fragments arranged by size: 4447 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2313 2343 1178 Size: 30 165 30 910 30 4512 Fragments arranged by size: 4512 910 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2742 1186 Size: 1556 4121 BseYI C'CCAG_C Cuts at: 2025 4880 5376 2025 Size: 2855 496 2326 Fragments arranged by size: 2855 2326 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2663 2700 1163 Size: 573 927 37 4140 Fragments arranged by size: 4140 927 573 37 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3127 3888 4916 1540 Size: 275 186 1126 761 1028 2301 Fragments arranged by size: 2301 1126 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2536 3738 3823 4894 2536 Size: 1202 85 1071 3319 Fragments arranged by size: 3319 1202 1071 85 BsmI GAATG_Cn' Cuts at: 3188 3281 3188 Size: 93 5584 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2536 2750 2996 3738 3823 4894 1391 Size: 399 746 214 246 742 85 1071 2174 Fragments arranged by size: 2174 1071 746 742 399 246 214 85 BspEI T'CCGG_A Cuts at: 5340 5340 Size: 5677 BspHI T'CATG_A Cuts at: 3566 5296 3566 Size: 1730 3947 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3032 844 Size: 2188 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2112 2948 3564 1394 Size: 429 289 836 616 3507 Fragments arranged by size: 3507 836 616 429 289 BsrDI GCAATG_nn' Cuts at: 65 1248 2788 4172 4346 65 Size: 1183 1540 1384 174 1396 Fragments arranged by size: 1540 1396 1384 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2147 2559 4321 4508 1700 Size: 110 337 412 1762 187 2869 Fragments arranged by size: 2869 1762 412 337 187 110 BsrGI T'GTAC_A Cuts at: 96 2260 96 Size: 2164 3513 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5677 BssSI C'ACGA_G Cuts at: 3737 4749 3737 Size: 1012 4665 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5677 BstBI TT'CG_AA Cuts at: 4481 4481 Size: 5677 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5677 BstYI r'GATC_y Cuts at: 1972 3007 3374 3759 3776 5228 5314 5326 5672 Size: 1035 367 385 17 1452 86 12 346 Cuts at: 5672 1972 Size: 1977 Fragments arranged by size: 1977 1452 1035 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5125 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2152 487 Size: 683 56 926 4012 Fragments arranged by size: 4012 926 683 56 BtsI GCAGTG_nn' Cuts at: 2314 3193 3987 4007 2314 Size: 879 794 20 3984 Fragments arranged by size: 3984 879 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5677 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5642 DraI TTT'AAA Cuts at: 2981 2981 Size: 5677 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2162 4684 809 Size: 507 846 2522 1802 Fragments arranged by size: 2522 1802 846 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3124 4013 5642 Size: 54 1425 50 275 18 1294 889 1629 Cuts at: 5642 8 Size: 43 Fragments arranged by size: 1629 1425 1294 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3124 1537 Size: 275 1312 4090 Fragments arranged by size: 4090 1312 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3607 1353 Size: 2254 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4264 4638 4784 387 Size: 1064 138 2675 374 146 1280 Fragments arranged by size: 2675 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3740 4337 5124 5392 1239 Size: 393 2108 597 787 268 1524 Fragments arranged by size: 2108 1524 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2569 2569 Size: 5677 EcoO109I rG'GnC_Cy Cuts at: 2384 2462 2746 2747 2384 Size: 78 284 1 5314 Fragments arranged by size: 5314 284 78 1 EcoRI G'AATT_C Cuts at: 2938 2986 3364 2938 Size: 48 378 5251 Fragments arranged by size: 5251 378 48 FspI TGC'GCA Cuts at: 4183 4183 Size: 5677 HaeII r_GCGC'y Cuts at: 1678 2070 2144 4824 1678 Size: 392 74 2680 2531 Fragments arranged by size: 2680 2531 392 74 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2806 2838 2927 2959 4318 Size: 32 135 32 1234 32 89 32 1359 Cuts at: 4318 4350 4392 4424 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1359 1234 135 89 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2618 3021 3267 669 Size: 988 281 680 403 246 3079 Fragments arranged by size: 3079 988 680 403 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5677 HpaI GTT'AAC Cuts at: 3267 3267 Size: 5677 KasI G'GCGC_C Cuts at: 2140 2140 Size: 5677 KpnI G_GTAC'C Cuts at: 2848 3002 2848 Size: 154 5523 MfeI C'AATT_G Cuts at: 3276 3276 Size: 5677 MluI A'CGCG_T Cuts at: 5553 5553 Size: 5677 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2655 4791 4975 1305 Size: 1350 2136 184 2007 Fragments arranged by size: 2136 2007 1350 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3855 Fragments arranged by size: 3855 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3635 3994 4153 518 Size: 648 2469 359 159 2042 Fragments arranged by size: 2469 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2100 2933 3093 3770 4918 5163 1606 Size: 494 833 160 677 1148 245 2120 Fragments arranged by size: 2120 1148 833 677 494 245 160 NaeI GCC'GGC Cuts at: 2149 2561 2149 Size: 412 5265 NarI GG'CG_CC Cuts at: 2141 2141 Size: 5677 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5125 NdeI CA'TA_TG Cuts at: 387 387 Size: 5677 NgoMIV G'CCGG_C Cuts at: 2147 2559 2147 Size: 412 5265 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5677 NotI GC'GGCC_GC Cuts at: 3124 3124 Size: 5677 NspI r_CATG'y Cuts at: 1132 1229 2599 3035 4580 1132 Size: 97 1370 436 1545 2229 Fragments arranged by size: 2229 1545 1370 436 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5677 PciI A'CATG_T Cuts at: 4576 4576 Size: 5677 PflMI CCAn_nnn'nTGG Cuts at: 1108 2900 1108 Size: 1792 3885 PmeI GTTT'AAAC Cuts at: 2981 2981 Size: 5677 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3746 3778 5259 5291 3746 Size: 32 1481 32 4132 Fragments arranged by size: 4132 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2384 2462 2384 Size: 78 5599 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5677 PsiI TTA'TAA Cuts at: 3247 3247 Size: 5677 PspOMI G'GGCC_C Cuts at: 2746 2746 Size: 5677 PstI C_TGCA'G Cuts at: 830 3029 830 Size: 2199 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5677 PvuII CAG'CTG Cuts at: 2933 2933 Size: 5677 SalI G'TCGA_C Cuts at: 1936 2616 3019 1936 Size: 680 403 4594 Fragments arranged by size: 4594 680 403 SbfI CC_TGCA'GG Cuts at: 3029 3029 Size: 5677 ScaI AGT'ACT Cuts at: 3925 5637 3925 Size: 1712 3965 SfcI C'TryA_G Cuts at: 826 1045 3025 4160 4841 5032 826 Size: 219 1980 1135 681 191 1471 Fragments arranged by size: 1980 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2124 2124 Size: 5677 SfoI GGC'GCC Cuts at: 2142 2142 Size: 5677 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5677 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5677 SmaI CCC'GGG Cuts at: 1872 2121 3004 1872 Size: 249 883 4545 Fragments arranged by size: 4545 883 249 SmlI C'TyrA_G Cuts at: 820 1017 1943 3781 4682 4944 820 Size: 197 926 1838 901 262 1553 Fragments arranged by size: 1838 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3468 493 Size: 2975 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5677 SphI G_CATG'C Cuts at: 1132 3035 1132 Size: 1903 3774 SrfI GCCC'GGGC Cuts at: 2121 2121 Size: 5677 SspI AAT'ATT Cuts at: 5 52 3601 5 Size: 47 3549 2081 Fragments arranged by size: 3549 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5677 StyI C'CwwG_G Cuts at: 513 1065 2244 2818 3116 513 Size: 552 1179 574 298 3074 Fragments arranged by size: 3074 1179 574 552 298 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3704 3721 3874 4059 4504 325 Size: 1255 2124 17 153 185 445 1498 Fragments arranged by size: 2124 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2260 3923 5635 Size: 274 80 33 51 1443 283 1663 1712 Cuts at: 5635 96 Size: 138 Fragments arranged by size: 1712 1663 1443 283 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2313 2345 5637 5669 2313 Size: 32 3292 32 2321 Fragments arranged by size: 3292 2321 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5677 XbaI T'CTAG_A Cuts at: 3013 3013 Size: 5677 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5677 XmaI C'CCGG_G Cuts at: 1870 2119 3002 1870 Size: 249 883 4545 Fragments arranged by size: 4545 883 249 XmnI GAAnn'nnTTC Cuts at: 3806 3806 Size: 5677 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3485 276 Size: 53 83 186 936 1951 2468 Fragments arranged by size: 2468 1951 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SwaI XcmI