Restriction map for
pFN21AB5242
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# NO CUT ENZYMES ( 118 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AhdI Alw44I
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
BalI BbeI BbsI BcgI BclI
BfrI BglII BlpI Bpu1102I BpuAI
BsaBI BsaHI BseAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrGI BssSI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I CelII
ClaI CpoI DraI DraIII DrdI
Eam1105I Eco47III EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI Hin1I HindIII
HpaI KasI KpnI MamI MfeI
MluI MluNI MroI MscI MseI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PflFI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SalI SbfI ScaI SexAI SfaNI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TfiI Tru9I Tsp509I Tth111I
XbaI XcmI XmnI
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# ONE CUT ENZYMES ( 29 enzymes) #
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AflIII 476 ACGTGT A^C[GA][CT]GT
AgeI 668 ACCGGT A^CCGGT
AviII 61 TGCGCA TGC^GCA
AvrII 1431 CCTAGG C^CTAGG
BamHI 824 GGATCC G^GATCC
BbvCI 1135 GCTGAGG CCTCAGC(-5/-2)
BciVI 1030 GGATAC GTATCC(6/5)
BlnI 1431 CCTAGG C^CTAGG
BsaWI 668 ACCGGT [AT]^CCGG[AT]
BsrBI 1423 GAGCGG CCGCTC(-3/-3)
BsrDI 699 CATTGC GCAATG(2/0)
BssHII 327 GCGCGC G^CGCGC
BstYI 824 GGATCC [GA]^GATC[CT]
EarI 276 GAAGAG CTCTTC(1/4)
FspI 61 TGCGCA TGC^GCA
HgaI 981 GCGTC GACGC(5/10)
HincII 865 GTCAAC GT[CT]^[GA]AC
HindII 865 GTCAAC GT[CT]^[GA]AC
Ksp632I 276 GAAGAG CTCTTC(1/4)
KspI 10 CCGCGG CCGC^GG
MboII 276 GAAGA GAAGA(8/7)
MflI 824 GGATCC [GA]^GATC[CT]
PinAI 668 ACCGGT A^CCGGT
PshAI 421 GACTTGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1150 CAGCTG CAG^CTG
SacI 1021 GAGCTC GAGCT^C
SacII 10 CCGCGG CCGC^GG
SapI 276 GAAGAGC GCTCTTC(1/4)
XhoII 824 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 37 enzymes) #
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AccII 11 CGCG CG^CG
328 CGCG
AocI 1416 CCTGAGG CC^T[ACGT]AGG
1515 CCTGAGG
AspHI 640 GTGCTC G[AT]GC[AT]^C
1021 GAGCTC
BbrPI 475 CACGTG CAC^GTG
631 CACGTG
BfaI 1316 CTAG C^TAG
1432 CTAG
BsaAI 475 CACGTG [CT]AC^GT[GA]
631 CACGTG
BsiHKAI 640 GTGCTC G[AT]GC[AT]^C
1021 GAGCTC
BsrFI 209 ACCGGC [GA]^CCGG[CT]
668 ACCGGT
BstF5I 438 CATCC GGATG(2/0)
471 CATCC
BstUI 11 CGCG CG^CG
328 CGCG
Bsu36I 1416 CCTGAGG CC^T[ACGT]AGG
1515 CCTGAGG
Cfr10I 209 ACCGGC [GA]^CCGG[CT]
668 ACCGGT
DpnII 162 GATC ^GATC
825 GATC
EagI 1007 CGGCCG C^GGCCG
1389 CGGCCG
EclXI 1007 CGGCCG C^GGCCG
1389 CGGCCG
Eco52I 1007 CGGCCG C^GGCCG
1389 CGGCCG
Eco81I 1416 CCTGAGG CC^T[ACGT]AGG
1515 CCTGAGG
EcoT14I 1285 CCAAGG C^C[AT][AT]GG
1431 CCTAGG
FokI 438 CATCC GGATG(9/13)
471 CATCC
HaeII 348 GGCGCT [GA]GCGC^[CT]
1318 AGCGCC
HphI 91 TCACC GGTGA(8/7)
1091 TCACC
MaeI 1316 CTAG C^TAG
1432 CTAG
MaeII 476 ACGT A^CGT
632 ACGT
MboI 162 GATC ^GATC
825 GATC
MvnI 11 CGCG CG^CG
328 CGCG
NdeII 162 GATC ^GATC
825 GATC
PaeR7I 1328 CTCGAG C^TCGAG
1407 CTCGAG
PmaCI 475 CACGTG CAC^GTG
631 CACGTG
PmlI 475 CACGTG CAC^GTG
631 CACGTG
PstI 253 CTGCAG CTGCA^G
415 CTGCAG
Sau3AI 162 GATC ^GATC
825 GATC
SmaI 29 CCCGGG CCC^GGG
70 CCCGGG
StuI 1434 AGGCCT AGG^CCT
1491 AGGCCT
StyI 1285 CCAAGG C^C[AT][AT]GG
1431 CCTAGG
XhoI 1328 CTCGAG C^TCGAG
1407 CTCGAG
XmaCI 29 CCCGGG C^CCGGG
70 CCCGGG
XmaI 29 CCCGGG C^CCGGG
70 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
ApaI AvaI AvaII BanI BanII
BbvI BcnI BglI BmyI BpmI
BsaI BsaJI BseRI BsgI BsiEI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstAPI
BstNI Cac8I CfoI Cfr13I DdeI
DraII DsaI EaeI EcoNI EcoO109I
EcoRII FauI Fnu4HI HaeIII HapII
HhaI HinP1I HinfI HpaII ItaI
MaeIII MnlI MslI MspA1I MspI
MvaI MwoI NciI NlaIII NlaIV
PflMI PleI PpuMI RsaI Sau96I
ScrFI SfcI SmlI TaqI TseI
Tsp45I TspRI TthHB8I Van91I