(Circular) (Six-base) MAPSORT of: pFN21AB5242.seq Check: 4724 from: 1 to: 6271 pFN21AB5242 6271 bp With 182 enzymes: SgfI * February 27, 2009 14:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4081 278 Size: 53 83 186 936 2545 2468 Fragments arranged by size: 2545 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3614 1937 Size: 1677 4594 Acc65I G'GTAC_C Cuts at: 3592 3592 Size: 6271 AclI AA'CG_TT Cuts at: 1563 4398 4771 1563 Size: 2835 373 3063 Fragments arranged by size: 3063 2835 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2896 3582 4334 5718 5986 1632 Size: 1264 686 752 1384 268 1917 Fragments arranged by size: 1917 1384 1264 752 686 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6074 AflIII A'CryG_T Cuts at: 1203 2481 5170 6147 1203 Size: 1278 2689 977 1327 Fragments arranged by size: 2689 1327 1278 977 AgeI A'CCGG_T Cuts at: 2673 5102 2673 Size: 2429 3842 AhdI GACnn_n'nnGTC Cuts at: 5000 5000 Size: 6271 AleI CACnn'nnGTG Cuts at: 1166 3014 1166 Size: 1848 4423 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6271 AlwNI CAG_nnn'CTG Cuts at: 2246 2662 2695 3160 3187 3460 3541 4014 5586 Size: 416 33 465 27 273 81 473 1572 Cuts at: 5586 2246 Size: 2931 Fragments arranged by size: 2931 1572 473 465 416 273 81 33 27 ApaI G_GGCC'C Cuts at: 2041 2076 2199 3421 2041 Size: 35 123 1222 4891 Fragments arranged by size: 4891 1222 123 35 ApaLI G'TGCA_C Cuts at: 4328 5484 4328 Size: 1156 5115 ApoI r'AATT_y Cuts at: 1493 3580 3806 3958 6067 1493 Size: 2087 226 152 2109 1697 Fragments arranged by size: 2109 2087 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4825 160 Size: 872 3793 1606 Fragments arranged by size: 3793 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6271 AvaI C'yCGr_G Cuts at: 1870 1943 2034 2041 2075 2838 3333 3412 3596 Size: 73 91 7 34 763 495 79 184 Cuts at: 3596 1870 Size: 4545 Fragments arranged by size: 4545 763 495 184 91 79 73 34 7 AvrII C'CTAG_G Cuts at: 3436 3436 Size: 6271 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2744 2777 3028 3061 2744 Size: 33 251 33 5954 Fragments arranged by size: 5954 251 33 33 BamHI G'GATC_C Cuts at: 2829 3601 3968 2829 Size: 772 367 5132 Fragments arranged by size: 5132 772 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2670 2854 2901 2963 3432 Size: 325 217 627 883 184 47 62 469 Cuts at: 3432 3592 5047 618 Size: 160 1455 1842 Fragments arranged by size: 1842 1455 883 627 469 325 217 184 160 62 47 BanII G_rGCy'C Cuts at: 1391 2041 2076 2199 2288 2688 2920 3030 3413 Size: 650 35 123 89 400 232 110 383 Cuts at: 3413 3421 3590 1391 Size: 8 169 4072 Fragments arranged by size: 4072 650 400 383 232 169 123 110 89 35 8 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5300 BbvCI CC'TCA_GC Cuts at: 3141 3141 Size: 6271 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4462 4496 1903 Size: 34 2525 34 3678 Fragments arranged by size: 3678 2525 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3029 4163 5373 3029 Size: 1134 1210 3927 Fragments arranged by size: 3927 1210 1134 BclI T'GATC_A Cuts at: 1548 1548 Size: 6271 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2776 3306 3393 Size: 107 122 71 631 605 1104 530 87 Cuts at: 3393 3447 4882 136 Size: 54 1435 1525 Fragments arranged by size: 1525 1435 1104 631 605 530 122 107 87 71 54 BglII A'GATC_T Cuts at: 6266 6266 Size: 6271 BlpI GC'TnA_GC Cuts at: 3688 3688 Size: 6271 Bme1580I G_kGCm'C Cuts at: 1790 2041 2076 2199 2542 3075 3109 3421 4332 Size: 251 35 123 343 533 34 312 911 Cuts at: 4332 5488 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 911 533 343 312 251 123 35 34 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6271 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2229 4002 4960 Size: 416 53 83 399 24 812 1773 958 Cuts at: 4960 442 Size: 1753 Fragments arranged by size: 1773 1753 958 812 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6271 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2138 2138 Size: 6271 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2157 3044 3183 3503 3518 4931 1239 Size: 918 887 139 320 15 1413 2579 Fragments arranged by size: 2579 1413 918 887 320 139 15 Bpu10I CC'TnA_GC Cuts at: 3141 3141 Size: 6271 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2449 2843 3494 4396 5261 5559 2449 Size: 394 651 902 865 298 3161 Fragments arranged by size: 3161 902 865 651 394 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2038 2094 2388 3330 4934 882 Size: 617 539 56 294 942 1604 2219 Fragments arranged by size: 2219 1604 942 617 539 294 56 BsaAI yAC'GTr Cuts at: 493 1204 2482 2638 4062 493 Size: 711 1278 156 1424 2702 Fragments arranged by size: 2702 1424 1278 711 156 BsaBI GATnn'nnATC Cuts at: 6265 6265 Size: 6271 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4078 4460 5095 275 Size: 53 83 186 936 2545 382 635 1451 Fragments arranged by size: 2545 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2673 4704 5102 5376 5523 5934 2673 Size: 2031 398 274 147 411 3010 Fragments arranged by size: 3010 2031 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2072 2102 2441 2471 3181 Size: 30 165 30 669 30 339 30 710 Cuts at: 3181 3211 1178 Size: 30 4238 Fragments arranged by size: 4238 710 669 339 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2023 2241 3182 1186 Size: 837 218 941 4275 Fragments arranged by size: 4275 941 837 218 BseYI C'CCAG_C Cuts at: 2198 2308 2321 2655 2688 3084 3160 3406 3504 Size: 110 13 334 33 396 76 246 98 Cuts at: 3504 3552 5474 5970 2198 Size: 48 1922 496 2499 Fragments arranged by size: 2499 1922 496 396 334 246 110 98 76 48 33 13 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2259 2474 2906 3193 1163 Size: 573 523 215 432 287 4241 Fragments arranged by size: 4241 573 523 432 287 215 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3015 3103 3397 3721 4482 5510 Size: 275 186 1014 88 294 324 761 1028 Cuts at: 5510 1540 Size: 2301 Fragments arranged by size: 2301 1028 1014 761 324 294 275 186 88 BsiHKAI G_wGCw'C Cuts at: 2649 3030 4332 4417 5488 2649 Size: 381 1302 85 1071 3432 Fragments arranged by size: 3432 1302 1071 381 85 BsmI GAATG_Cn' Cuts at: 3782 3875 3782 Size: 93 6178 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2041 2076 2199 2288 2542 2649 2688 Size: 399 251 35 123 89 254 107 39 Cuts at: 2688 2920 3030 3075 3109 3413 3421 3590 4332 Size: 232 110 45 34 304 8 169 742 Cuts at: 4332 4417 5488 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 304 254 251 232 169 123 110 107 89 85 45 39 35 34 8 BspEI T'CCGG_A Cuts at: 5934 5934 Size: 6271 BspHI T'CATG_A Cuts at: 4160 5890 4160 Size: 1730 4541 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2072 2231 2401 2799 3626 844 Size: 1228 159 170 398 827 3489 Fragments arranged by size: 3489 1228 827 398 170 159 BsrBI CCG'CTC Cuts at: 1394 1823 3430 4158 1394 Size: 429 1607 728 3507 Fragments arranged by size: 3507 1607 728 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2703 4766 4940 65 Size: 1183 1455 2063 174 1396 Fragments arranged by size: 2063 1455 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2214 2673 4915 5102 1700 Size: 110 404 459 2242 187 2869 Fragments arranged by size: 2869 2242 459 404 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6271 BssHII G'CGCG_C Cuts at: 1925 2332 1925 Size: 407 5864 BssSI C'ACGA_G Cuts at: 4331 5343 4331 Size: 1012 5259 BstAPI GCAn_nnn'nTGC Cuts at: 794 2257 2677 2812 3070 794 Size: 1463 420 135 258 3995 Fragments arranged by size: 3995 1463 420 258 135 BstBI TT'CG_AA Cuts at: 5075 5075 Size: 6271 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6271 BstYI r'GATC_y Cuts at: 1972 2829 3601 3968 4353 4370 5822 5908 5920 Size: 857 772 367 385 17 1452 86 12 Cuts at: 5920 6266 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 857 772 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3422 3521 3422 Size: 99 6172 BtgI C'CryG_G Cuts at: 513 1065 2015 2390 3015 513 Size: 552 950 375 625 3769 Fragments arranged by size: 3769 950 625 552 375 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5532 Fragments arranged by size: 5532 683 56 BtsI GCAGTG_nn' Cuts at: 2417 3787 4581 4601 2417 Size: 1370 794 20 4087 Fragments arranged by size: 4087 1370 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6271 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6236 DraI TTT'AAA Cuts at: 3575 3575 Size: 6271 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5278 809 Size: 507 3962 1802 Fragments arranged by size: 3962 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2387 3012 3394 Size: 54 1425 50 275 18 557 625 382 Cuts at: 3394 3718 4607 6236 8 Size: 324 889 1629 43 Fragments arranged by size: 1629 1425 889 625 557 382 324 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3012 3394 3718 1537 Size: 275 1200 382 324 4090 Fragments arranged by size: 4090 1200 382 324 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2276 4201 1353 Size: 923 1925 3423 Fragments arranged by size: 3423 1925 923 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2552 4858 5232 5378 387 Size: 1064 138 963 2306 374 146 1280 Fragments arranged by size: 2306 1280 1064 963 374 146 138 EcoICRI GAG'CTC Cuts at: 3028 3028 Size: 6271 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2157 2896 3044 3183 3503 3518 3582 Size: 393 525 739 148 139 320 15 64 Cuts at: 3582 4334 4931 5718 5986 1239 Size: 752 597 787 268 1524 Fragments arranged by size: 1524 787 752 739 597 525 393 320 268 148 139 64 15 EcoNI CCTnn'n_nnAGG Cuts at: 2223 2250 2546 2223 Size: 27 296 5948 Fragments arranged by size: 5948 296 27 EcoO109I rG'GnC_Cy Cuts at: 2038 2072 2195 2231 2243 3044 3184 3261 3268 Size: 34 123 36 12 801 140 77 7 Cuts at: 3268 3294 3417 3418 3538 3557 2038 Size: 26 123 1 120 19 4752 Fragments arranged by size: 4752 801 140 123 123 120 77 36 34 26 19 12 7 1 EcoRI G'AATT_C Cuts at: 3580 3958 3580 Size: 378 5893 FspI TGC'GCA Cuts at: 2068 4777 2068 Size: 2709 3562 HaeII r_GCGC'y Cuts at: 1678 2357 3327 5418 1678 Size: 679 970 2091 2531 Fragments arranged by size: 2531 2091 970 679 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2072 2104 2821 2853 2971 Size: 32 135 32 500 32 717 32 118 Cuts at: 2971 3003 3181 3213 4912 4944 4986 5018 1373 Size: 32 178 32 1699 32 42 32 2626 Fragments arranged by size: 2626 1699 717 500 178 135 118 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2872 3615 3861 669 Size: 988 281 934 743 246 3079 Fragments arranged by size: 3079 988 934 743 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6271 HpaI GTT'AAC Cuts at: 3861 3861 Size: 6271 KpnI G_GTAC'C Cuts at: 3596 3596 Size: 6271 MfeI C'AATT_G Cuts at: 3870 3870 Size: 6271 MluI A'CGCG_T Cuts at: 6147 6147 Size: 6271 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2203 5385 5569 1305 Size: 898 3182 184 2007 Fragments arranged by size: 3182 2007 898 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4449 Fragments arranged by size: 4449 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2480 2879 3014 4229 4588 4747 518 Size: 648 1314 399 135 1215 359 159 2042 Fragments arranged by size: 2042 1314 1215 648 399 359 159 135 MspA1I CmG'CkG Cuts at: 1606 2017 2263 3111 3157 3687 4364 5512 5757 Size: 411 246 848 46 530 677 1148 245 Cuts at: 5757 1606 Size: 2120 Fragments arranged by size: 2120 1148 848 677 530 411 246 245 46 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5719 NdeI CA'TA_TG Cuts at: 387 387 Size: 6271 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6271 NotI GC'GGCC_GC Cuts at: 3718 3718 Size: 6271 NspI r_CATG'y Cuts at: 1132 1229 3629 5174 1132 Size: 97 2400 1545 2229 Fragments arranged by size: 2400 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2288 3541 1348 Size: 940 1253 4078 Fragments arranged by size: 4078 1253 940 PciI A'CATG_T Cuts at: 5170 5170 Size: 6271 PflMI CCAn_nnn'nTGG Cuts at: 1108 2205 2377 2522 2641 2662 2695 2716 3160 Size: 1097 172 145 119 21 33 21 444 Cuts at: 3160 3352 3541 1108 Size: 192 189 3838 Fragments arranged by size: 3838 1097 444 192 189 172 145 119 33 21 21 PmeI GTTT'AAAC Cuts at: 3575 3575 Size: 6271 PmlI CAC'GTG Cuts at: 2482 2638 2482 Size: 156 6115 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4340 4372 5853 5885 4340 Size: 32 1481 32 4726 Fragments arranged by size: 4726 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2243 3044 3184 3261 3268 3294 2243 Size: 801 140 77 7 26 5220 Fragments arranged by size: 5220 801 140 77 26 7 PshAI GACnn'nnGTC Cuts at: 1613 2430 1613 Size: 817 5454 PsiI TTA'TAA Cuts at: 3841 3841 Size: 6271 PspOMI G'GGCC_C Cuts at: 2037 2072 2195 3417 2037 Size: 35 123 1222 4891 Fragments arranged by size: 4891 1222 123 35 PspXI vC'TCGA_Gb Cuts at: 3412 3412 Size: 6271 PstI C_TGCA'G Cuts at: 830 2262 2424 3623 830 Size: 1432 162 1199 3478 Fragments arranged by size: 3478 1432 1199 162 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6271 PvuII CAG'CTG Cuts at: 3157 3157 Size: 6271 SacI G_AGCT'C Cuts at: 3030 3030 Size: 6271 SacII CC_GC'GG Cuts at: 2018 2018 Size: 6271 SalI G'TCGA_C Cuts at: 1936 3613 1936 Size: 1677 4594 SanDI GG'GwC_CC Cuts at: 3044 3044 Size: 6271 SapI GCTCTTCn'nnn_ Cuts at: 2276 2276 Size: 6271 SbfI CC_TGCA'GG Cuts at: 3623 3623 Size: 6271 ScaI AGT'ACT Cuts at: 4519 6231 4519 Size: 1712 4559 SfcI C'TryA_G Cuts at: 826 1045 2258 2420 2865 3619 4754 5435 5626 Size: 219 1213 162 445 754 1135 681 191 Cuts at: 5626 826 Size: 1471 Fragments arranged by size: 1471 1213 1135 754 681 445 219 191 162 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6271 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6271 SmaI CCC'GGG Cuts at: 1872 2036 2077 3598 1872 Size: 164 41 1521 4545 Fragments arranged by size: 4545 1521 164 41 SmlI C'TyrA_G Cuts at: 820 1017 1943 2428 2822 3333 3412 3509 4375 Size: 197 926 485 394 511 79 97 866 Cuts at: 4375 5276 5538 820 Size: 901 262 1553 Fragments arranged by size: 1553 926 901 866 511 485 394 262 197 97 79 SnaBI TAC'GTA Cuts at: 493 4062 493 Size: 3569 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6271 SphI G_CATG'C Cuts at: 1132 3629 1132 Size: 2497 3774 SspI AAT'ATT Cuts at: 5 52 4195 5 Size: 47 4143 2081 Fragments arranged by size: 4143 2081 47 StuI AGG'CCT Cuts at: 1517 3441 3498 1517 Size: 1924 57 4290 Fragments arranged by size: 4290 1924 57 StyI C'CwwG_G Cuts at: 513 1065 3290 3436 3710 513 Size: 552 2225 146 274 3074 Fragments arranged by size: 3074 2225 552 274 146 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2062 2113 4298 4315 4468 4653 5098 Size: 1255 482 51 2185 17 153 185 445 Cuts at: 5098 325 Size: 1498 Fragments arranged by size: 2185 1498 1255 482 445 185 153 51 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2176 3117 4517 Size: 274 80 33 51 1443 199 941 1400 Cuts at: 4517 6229 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1400 941 274 199 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6231 6263 6231 Size: 32 6239 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6271 XbaI T'CTAG_A Cuts at: 3607 3607 Size: 6271 XhoI C'TCGA_G Cuts at: 1943 3333 3412 1943 Size: 1390 79 4802 Fragments arranged by size: 4802 1390 79 XmaI C'CCGG_G Cuts at: 1870 2034 2075 3596 1870 Size: 164 41 1521 4545 Fragments arranged by size: 4545 1521 164 41 XmnI GAAnn'nnTTC Cuts at: 4400 4400 Size: 6271 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4079 276 Size: 53 83 186 936 2545 2468 Fragments arranged by size: 2545 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PsrI RsrII SexAI SfiI SfoI SrfI SwaI XcmI