Restriction map for
pFN21AB5208
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# NO CUT ENZYMES ( 143 enzymes) #
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AatII AccI AccII AcsI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
AspEI AspI AvaI AviII AvrII
BamHI BbeI BbrPI BbvCI BcgI
BciVI BfaI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BsaAI
BsaBI BsaHI BsiWI BsmFI BsmI
BsoBI Bsp1407I BspDI BspLU11I BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstUI BstYI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoRI EcoRV
EcoT22I FauI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HindIII
HpaI KasI KspI MaeI MamI
MfeI MflI MluI MunI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI PstI PvuI
RsrII SacII SalI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tsp509I Tth111I XbaI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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Acc65I 654 GGTACC G^GTACC
AccIII 548 TCCGGA T^CCGGA
AclI 826 AACGTT AA^CGTT
Asp700I 960 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 654 GGTACC G^GTACC
AspHI 268 GAGCTC G[AT]GC[AT]^C
BanI 654 GGTACC G^G[CT][GA]CC
BclI 482 TGATCA T^GATCA
BsaWI 548 TCCGGA [AT]^CCGG[AT]
BseAI 548 TCCGGA T^CCGGA
BsgI 576 CTGCAC GTGCAG(16/14)
BsiEI 414 CGACCG CG[GA][CT]^CG
BsiHKAI 268 GAGCTC G[AT]GC[AT]^C
BsmBI 837 CGTCTC CGTCTC(1/5)
BspEI 548 TCCGGA T^CCGGA
BspMI 303 GCAGGT ACCTGC(4/8)
BsrDI 125 GCAATG GCAATG(2/0)
BstAPI 386 GCATCTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 759 GGTCACC G^GT[ACGT]ACC
BstPI 759 GGTCACC G^GT[ACGT]ACC
BstXI 776 CCATCGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 803 GCTGGC GC[ACGT]^[ACGT]GC
DpnII 483 GATC ^GATC
DraII 1072 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoNI 731 CCTCTAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1072 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 759 GGTCACC G^GT[ACGT]ACC
FbaI 482 TGATCA T^GATCA
HincII 78 GTCAAC GT[CT]^[GA]AC
HindII 78 GTCAAC GT[CT]^[GA]AC
KpnI 654 GGTACC GGTAC^C
MaeII 827 ACGT A^CGT
MboI 483 GATC ^GATC
MroI 548 TCCGGA T^CCGGA
MseI 869 TTAA T^TAA
NdeII 483 GATC ^GATC
NspI 875 GCATGT [GA]CATG^[CT]
PleI 503 GAGTC GAGTC(4/5)
PpuMI 1072 AGGACCC [GA]G^G[AT]CC[CT]
Psp1406I 826 AACGTT AA^CGTT
SacI 268 GAGCTC GAGCT^C
Sau3AI 483 GATC ^GATC
TaqI 413 TCGA T^CGA
Tru9I 869 TTAA T^TAA
TthHB8I 413 TCGA T^CGA
XmnI 960 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
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BbsI 222 GAAGAC GAAGAC(2/6)
790 GTCTTC
BbvI 572 GCAGC GCAGC(8/12)
575 GCTGC
BpuAI 222 GAAGAC GAAGAC(2/6)
790 GTCTTC
BsrBI 996 GAGCGG CCGCTC(-3/-3)
1015 GAGCGG
DdeI 734 CTAAG C^T[ACGT]AG
749 CTGAG
EarI 271 CTCTTC CTCTTC(1/4)
960 GAAGAG
EcoT14I 488 CCATGG C^C[AT][AT]GG
842 CCATGG
Ksp632I 271 CTCTTC CTCTTC(1/4)
960 GAAGAG
MaeIII 760 GTCAC ^GT[ACGT]AC
1022 GTGAC
MspA1I 573 CAGCTG C[AC]G^C[GT]G
912 CAGCTG
NcoI 488 CCATGG C^CATGG
842 CCATGG
PflMI 19 CCACTCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
581 CCATCAACTGG
PvuII 573 CAGCTG CAG^CTG
912 CAGCTG
SapI 960 GAAGAGC GCTCTTC(1/4)
270 GCTCTTC
StyI 488 CCATGG C^C[AT][AT]GG
842 CCATGG
TfiI 598 GAATC G^A[AT]TC
716 GAATC
TseI 572 GCAGC G^C[AT]GC
575 GCTGC
Tsp45I 760 GTCAC ^GT[GC]AC
1022 GTGAC
TspRI 319 CACTG CA[GC]TG[ACGT][ACGT]^
559 CAGTG
Van91I 19 CCACTCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
581 CCATCAACTGG
XcmI 318 CCACTGGCCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
338 CCATTTGCCGGGTGG
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# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AfaI AluI AvaII BalI
BanII BcnI BmyI BsaI BsaJI
BseRI BsiYI BslI BsmAI Bsp1286I
BspHI BsrI BssKI BstF5I BstNI
Cfr13I DsaI EaeI EcoRII Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI MboII MluNI MnlI
MscI MslI MspI MvaI MwoI
NciI NlaIII NlaIV RcaI RsaI
Sau96I ScrFI SfaNI