(Circular) (Six-base) MAPSORT of: pFN21AB5208.seq Check: 5257 from: 1 to: 5812 pFN21AB5208 5812 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2299 2299 Size: 5812 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3622 278 Size: 53 83 186 936 2086 2468 Fragments arranged by size: 2468 2086 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3155 1937 Size: 1218 4594 Acc65I G'GTAC_C Cuts at: 2659 3133 2659 Size: 474 5338 AclI AA'CG_TT Cuts at: 1563 2832 3939 4312 1563 Size: 1269 1107 373 3063 Fragments arranged by size: 3063 1269 1107 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3875 5259 5527 1632 Size: 2243 1384 268 1917 Fragments arranged by size: 2243 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5615 AflIII A'CryG_T Cuts at: 1203 4711 5688 1203 Size: 3508 977 1327 Fragments arranged by size: 3508 1327 977 AgeI A'CCGG_T Cuts at: 4643 4643 Size: 5812 AhdI GACnn_n'nnGTC Cuts at: 4541 4541 Size: 5812 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5812 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5812 AlwNI CAG_nnn'CTG Cuts at: 3555 5127 3555 Size: 1572 4240 ApaLI G'TGCA_C Cuts at: 3869 5025 3869 Size: 1156 4656 ApoI r'AATT_y Cuts at: 1493 3121 3347 3499 5608 1493 Size: 1628 226 152 2109 1697 Fragments arranged by size: 2109 1697 1628 226 152 AseI AT'TA_AT Cuts at: 160 1032 4366 160 Size: 872 3334 1606 Fragments arranged by size: 3334 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5812 AvaI C'yCGr_G Cuts at: 1870 1943 3137 1870 Size: 73 1194 4545 Fragments arranged by size: 4545 1194 73 BamHI G'GATC_C Cuts at: 3142 3509 3142 Size: 367 5445 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2659 3133 4588 618 Size: 325 217 627 872 474 1455 1842 Fragments arranged by size: 1842 1455 872 627 474 325 217 BanII G_rGCy'C Cuts at: 1391 2277 2553 2735 3131 1391 Size: 886 276 182 396 4072 Fragments arranged by size: 4072 886 396 276 182 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2234 2788 928 Size: 971 335 554 3952 Fragments arranged by size: 3952 971 554 335 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4003 4037 1903 Size: 34 2066 34 3678 Fragments arranged by size: 3678 2066 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3704 4914 3704 Size: 1210 4602 BclI T'GATC_A Cuts at: 1548 2487 1548 Size: 939 4873 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4423 136 Size: 107 122 71 631 605 2751 1525 Fragments arranged by size: 2751 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5807 5807 Size: 5812 BlpI GC'TnA_GC Cuts at: 3229 3229 Size: 5812 Bme1580I G_kGCm'C Cuts at: 1790 3873 5029 1790 Size: 2083 1156 2573 Fragments arranged by size: 2573 2083 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5812 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3543 4501 442 Size: 416 53 83 399 24 2126 958 1753 Fragments arranged by size: 2126 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5812 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4472 1239 Size: 3233 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3937 4802 5100 3937 Size: 865 298 4649 Fragments arranged by size: 4649 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2461 3053 3062 3087 4475 882 Size: 617 962 592 9 25 1388 2219 Fragments arranged by size: 2219 1388 962 617 592 25 9 BsaAI yAC'GTr Cuts at: 493 1204 3603 493 Size: 711 2399 2702 Fragments arranged by size: 2702 2399 711 BsaBI GATnn'nnATC Cuts at: 5806 5806 Size: 5812 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3619 4001 4636 275 Size: 53 83 186 936 2086 382 635 1451 Fragments arranged by size: 2086 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2553 4245 4643 4917 5064 5475 2553 Size: 1692 398 274 147 411 2890 Fragments arranged by size: 2890 1692 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3016 3046 1178 Size: 30 165 30 1613 30 3944 Fragments arranged by size: 3944 1613 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2479 2725 3074 1186 Size: 1293 246 349 3924 Fragments arranged by size: 3924 1293 349 246 BseYI C'CCAG_C Cuts at: 2284 2546 2866 5015 5511 2284 Size: 262 320 2149 496 2585 Fragments arranged by size: 2585 2149 496 320 262 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2566 1163 Size: 573 830 4409 Fragments arranged by size: 4409 830 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2422 3262 4023 5051 1540 Size: 275 186 421 840 761 1028 2301 Fragments arranged by size: 2301 1028 840 761 421 275 186 BsiHKAI G_wGCw'C Cuts at: 2277 3873 3958 5029 2277 Size: 1596 85 1071 3060 Fragments arranged by size: 3060 1596 1071 85 BsmI GAATG_Cn' Cuts at: 3323 3416 3323 Size: 93 5719 BsmBI CGTCTCn'nnnn_ Cuts at: 2848 2848 Size: 5812 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2277 2553 2735 3131 3873 3958 5029 Size: 399 487 276 182 396 742 85 1071 Cuts at: 5029 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 487 399 396 276 182 85 BspEI T'CCGG_A Cuts at: 2553 5475 2553 Size: 2922 2890 BspHI T'CATG_A Cuts at: 2358 2409 2535 3701 5431 2358 Size: 51 126 1166 1730 2739 Fragments arranged by size: 2739 1730 1166 126 51 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2299 3167 844 Size: 1455 868 3489 Fragments arranged by size: 3489 1455 868 BsrBI CCG'CTC Cuts at: 1394 1823 3003 3022 3699 1394 Size: 429 1180 19 677 3507 Fragments arranged by size: 3507 1180 677 429 19 BsrDI GCAATG_nn' Cuts at: 65 1248 2137 4307 4481 65 Size: 1183 889 2170 174 1396 Fragments arranged by size: 2170 1396 1183 889 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4456 4643 1700 Size: 110 2646 187 2869 Fragments arranged by size: 2869 2646 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5812 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5812 BssSI C'ACGA_G Cuts at: 3872 4884 3872 Size: 1012 4800 BstAPI GCAn_nnn'nTGC Cuts at: 794 2397 794 Size: 1603 4209 BstBI TT'CG_AA Cuts at: 4616 4616 Size: 5812 BstEII G'GTnAC_C Cuts at: 1184 2764 1184 Size: 1580 4232 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2788 2011 Size: 777 5035 BstYI r'GATC_y Cuts at: 1972 3142 3509 3894 3911 5363 5449 5461 5807 Size: 1170 367 385 17 1452 86 12 346 Cuts at: 5807 1972 Size: 1977 Fragments arranged by size: 1977 1452 1170 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2493 2847 3063 513 Size: 552 939 489 354 216 3262 Fragments arranged by size: 3262 939 552 489 354 216 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2248 487 Size: 683 56 1022 4051 Fragments arranged by size: 4051 1022 683 56 BtsI GCAGTG_nn' Cuts at: 3328 4122 4142 3328 Size: 794 20 4998 Fragments arranged by size: 4998 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5812 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5777 DraI TTT'AAA Cuts at: 3116 3116 Size: 5812 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4819 809 Size: 507 3503 1802 Fragments arranged by size: 3503 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2259 2454 2478 Size: 54 1425 50 275 18 429 195 24 Cuts at: 2478 2594 2850 3259 4148 5777 8 Size: 116 256 409 889 1629 43 Fragments arranged by size: 1629 1425 889 429 409 275 256 195 116 54 50 43 24 18 EagI C'GGCC_G Cuts at: 1537 1812 3259 1537 Size: 275 1447 4090 Fragments arranged by size: 4090 1447 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2282 2960 3742 1353 Size: 929 678 782 3423 Fragments arranged by size: 3423 929 782 678 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2030 4399 4773 4919 387 Size: 1064 138 441 2369 374 146 1280 Fragments arranged by size: 2369 1280 1064 441 374 146 138 EcoICRI GAG'CTC Cuts at: 2275 2275 Size: 5812 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3875 4472 5259 5527 1239 Size: 393 2243 597 787 268 1524 Fragments arranged by size: 2243 1524 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2740 2740 Size: 5812 EcoO109I rG'GnC_Cy Cuts at: 3078 3078 Size: 5812 EcoRI G'AATT_C Cuts at: 3121 3499 3121 Size: 378 5434 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2986 2986 Size: 5812 FspI TGC'GCA Cuts at: 4318 4318 Size: 5812 HaeII r_GCGC'y Cuts at: 1678 4959 1678 Size: 3281 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4453 4485 4527 4559 1373 Size: 32 135 32 2881 32 42 32 2626 Fragments arranged by size: 2881 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2085 3156 3402 669 Size: 988 281 147 1071 246 3079 Fragments arranged by size: 3079 1071 988 281 246 147 HindIII A'AGCT_T Cuts at: 748 748 Size: 5812 HpaI GTT'AAC Cuts at: 3402 3402 Size: 5812 KpnI G_GTAC'C Cuts at: 2663 3137 2663 Size: 474 5338 MfeI C'AATT_G Cuts at: 3411 3411 Size: 5812 MluI A'CGCG_T Cuts at: 5688 5688 Size: 5812 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4926 5110 1305 Size: 3621 184 2007 Fragments arranged by size: 3621 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2261 2596 2852 10 Size: 54 1425 343 429 335 256 2970 Fragments arranged by size: 2970 1425 429 343 335 256 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2363 2498 2705 2771 2786 3770 Size: 648 843 354 135 207 66 15 984 Cuts at: 3770 4129 4288 518 Size: 359 159 2042 Fragments arranged by size: 2042 984 843 648 359 354 207 159 135 66 15 MspA1I CmG'CkG Cuts at: 1606 2580 2919 3228 3905 5053 5298 1606 Size: 974 339 309 677 1148 245 2120 Fragments arranged by size: 2120 1148 974 677 339 309 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2493 2847 513 Size: 552 939 489 354 3478 Fragments arranged by size: 3478 939 552 489 354 NdeI CA'TA_TG Cuts at: 387 387 Size: 5812 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5812 NotI GC'GGCC_GC Cuts at: 3259 3259 Size: 5812 NspI r_CATG'y Cuts at: 1132 1229 2884 3170 4715 1132 Size: 97 1655 286 1545 2229 Fragments arranged by size: 2229 1655 1545 286 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5812 PciI A'CATG_T Cuts at: 4711 4711 Size: 5812 PflMI CCAn_nnn'nTGG Cuts at: 1108 2030 2592 1108 Size: 922 562 4328 Fragments arranged by size: 4328 922 562 PmeI GTTT'AAAC Cuts at: 3116 3116 Size: 5812 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3881 3913 5394 5426 3881 Size: 32 1481 32 4267 Fragments arranged by size: 4267 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3078 3078 Size: 5812 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5812 PsiI TTA'TAA Cuts at: 3382 3382 Size: 5812 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2609 2641 2609 Size: 32 5780 PstI C_TGCA'G Cuts at: 830 3164 830 Size: 2334 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5812 PvuII CAG'CTG Cuts at: 2580 2919 2580 Size: 339 5473 SacI G_AGCT'C Cuts at: 2277 2277 Size: 5812 SalI G'TCGA_C Cuts at: 1936 3154 1936 Size: 1218 4594 SapI GCTCTTCn'nnn_ Cuts at: 2282 2960 2282 Size: 678 5134 SbfI CC_TGCA'GG Cuts at: 3164 3164 Size: 5812 ScaI AGT'ACT Cuts at: 4060 5772 4060 Size: 1712 4100 SfcI C'TryA_G Cuts at: 826 1045 3160 4295 4976 5167 826 Size: 219 2115 1135 681 191 1471 Fragments arranged by size: 2115 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5812 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5812 SmaI CCC'GGG Cuts at: 1872 3139 1872 Size: 1267 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3916 4817 5079 820 Size: 197 926 1973 901 262 1553 Fragments arranged by size: 1973 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3603 493 Size: 3110 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5812 SphI G_CATG'C Cuts at: 1132 3170 1132 Size: 2038 3774 SspI AAT'ATT Cuts at: 5 52 3736 5 Size: 47 3684 2081 Fragments arranged by size: 3684 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5812 StyI C'CwwG_G Cuts at: 513 1065 2004 2493 2847 3251 513 Size: 552 939 489 354 404 3074 Fragments arranged by size: 3074 939 552 489 404 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2436 3839 3856 4009 4194 4639 325 Size: 1255 856 1403 17 153 185 445 1498 Fragments arranged by size: 1498 1403 1255 856 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4058 5770 96 Size: 274 80 33 51 1443 2081 1712 138 Fragments arranged by size: 2081 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2712 2744 5772 5804 2712 Size: 32 3028 32 2720 Fragments arranged by size: 3028 2720 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5812 XbaI T'CTAG_A Cuts at: 3148 3148 Size: 5812 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2330 2350 2330 Size: 20 5792 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5812 XmaI C'CCGG_G Cuts at: 1870 3137 1870 Size: 1267 4545 XmnI GAAnn'nnTTC Cuts at: 2969 3941 2969 Size: 972 4840 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3620 276 Size: 53 83 186 936 2086 2468 Fragments arranged by size: 2468 2086 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI