Restriction map for
pFN21AB5199
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# NO CUT ENZYMES ( 119 enzymes) #
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Acc65I AccIII AclI AcsI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AviII AvrII BamHI BbeI BbrPI
BbvCI BcgI BciVI BclI BfrI
BlnI BsaBI BsaWI BseAI BsiWI
BsmI BspDI BspEI BspHI BspLU11I
BsrDI BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I ClaI
CpoI DraI DrdI Eam1105I Eco47III
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KasI KpnI
MamI MfeI MluI MroI MseI
MunI NarI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
Sse8387I SspI StuI SwaI Tru9I
Tsp509I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AatII 931 GACGTC GACGT^C
AlwNI 1114 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 601 GAGCAC G[AT]GC[AT]^C
AspI 571 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1149 CCCGGG C^[CT]CG[GA]G
BbsI 955 GTCTTC GAAGAC(2/6)
BfaI 844 CTAG C^TAG
BglII 110 AGATCT A^GATCT
BlpI 708 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 708 GCTCAGC GC^T[ACGT]AGC
BpuAI 955 GTCTTC GAAGAC(2/6)
BsaAI 91 TACGTG [CT]AC^GT[GA]
BsiEI 1319 CGGCCG CG[GA][CT]^CG
BsiHKAI 601 GAGCAC G[AT]GC[AT]^C
BsmBI 1198 GAGACG CGTCTC(1/5)
BsoBI 1149 CCCGGG C^[CT]CG[GA]G
BspMI 725 ACCTGC ACCTGC(4/8)
BssHII 44 GCGCGC G^CGCGC
BstXI 872 CCACGCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 708 GCTCAGC GC^T[ACGT]AGC
DraII 180 AGGCCCC [GA]G^G[ACGT]CC[CT]
DraIII 403 CACGACGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1319 CGGCCG C^GGCCG
EarI 1084 CTCTTC CTCTTC(1/4)
EclXI 1319 CGGCCG C^GGCCG
Eco52I 1319 CGGCCG C^GGCCG
EcoO109I 180 AGGCCCC [GA]G^G[ACGT]CC[CT]
HaeII 1139 AGCGCC [GA]GCGC^[CT]
HinfI 470 GAATC G^A[ACGT]TC
Ksp632I 1084 CTCTTC CTCTTC(1/4)
MaeI 844 CTAG C^TAG
NcoI 515 CCATGG C^CATGG
PflFI 571 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 628 CAGCTG CAG^CTG
SmaI 1149 CCCGGG CCC^GGG
SphI 533 GCATGC GCATG^C
TfiI 470 GAATC G^A[AT]TC
Tth111I 571 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1149 CCCGGG C^CCGGG
XmaI 1149 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 577 GTCTAC GT^[AC][GT]AC
934 GTCTAC
AcyI 931 GACGTC G[GA]^CG[CT]C
1105 GGCGTC
AvaII 498 GGACC G^G[AT]CC
540 GGACC
BanI 61 GGCACC G^G[CT][GA]CC
825 GGTGCC
BanII 96 GGGCTC G[GA]GC[CT]^C
1164 GGGCTC
BsaHI 931 GACGTC G[GA]^CG[CT]C
1105 GGCGTC
BseRI 37 CTCCTC GAGGAG(10/8)
894 CTCCTC
BsgI 271 CTGCAC GTGCAG(16/14)
715 CTGCAC
BsmFI 287 GGGAC GGGAC(10/14)
1147 GTCCC
Bsp1407I 458 TGTACA T^GTACA
746 TGTACA
BsrGI 458 TGTACA T^GTACA
746 TGTACA
BstYI 110 AGATCT [GA]^GATC[CT]
944 GGATCT
EcoT14I 515 CCATGG C^C[AT][AT]GG
1028 CCAAGG
Hin1I 931 GACGTC G[GA]^CG[CT]C
1105 GGCGTC
KspI 57 CCGCGG CCGC^GG
738 CCGCGG
MflI 110 AGATCT [GA]^GATC[CT]
944 GGATCT
NspI 533 GCATGC [GA]CATG^[CT]
764 ACATGC
PflMI 609 CCACGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
788 CCATGTCCTGG
PstI 436 CTGCAG CTGCA^G
625 CTGCAG
SacII 57 CCGCGG CCGC^GG
738 CCGCGG
SspBI 458 TGTACA T^GTACA
746 TGTACA
StyI 515 CCATGG C^C[AT][AT]GG
1028 CCAAGG
TaqI 224 TCGA T^CGA
1265 TCGA
Tsp45I 409 GTGAC ^GT[GC]AC
1108 GTCAC
TthHB8I 224 TCGA T^CGA
1265 TCGA
Van91I 609 CCACGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
788 CCATGTCCTGG
XhoII 110 AGATCT [GA]^GATC[CT]
944 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
BalI BbvI BcnI BglI BmyI
BpmI BsaI BsaJI BsiYI BslI
BsmAI Bsp1286I BsrBI BsrFI BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DsaI EaeI EcoRII FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HpaII HphI ItaI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TseI
TspRI XcmI