Restriction map for
pFN21AB5154
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# NO CUT ENZYMES ( 107 enzymes) #
##################################
Acc65I AccII AclI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI Asp700I Asp718I
AspEI AspI AviII BamHI BbvCI
BcgI BclI BfrI BglI BsiWI
BsmI BspDI BspLU11I BspMI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstZ17I Bsu36I ClaI CpoI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HgaI
HincII HindII HindIII HpaI KpnI
KspI MfeI MluI MunI MvnI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmeI
PshAI Psp1406I PstI PvuI PvuII
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SmlI SnaBI SphI Sse8387I StuI
Tth111I Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccIII 476 TCCGGA T^CCGGA
AseI 1468 ATTAAT AT^TAAT
AsnI 1468 ATTAAT AT^TAAT
AvaI 1063 CCCGAG C^[CT]CG[GA]G
AvrII 1329 CCTAGG C^CTAGG
BbeI 1798 GGCGCC GGCGC^C
BbrPI 1837 CACGTG CAC^GTG
BglII 1178 AGATCT A^GATCT
BlnI 1329 CCTAGG C^CTAGG
BsaAI 1837 CACGTG [CT]AC^GT[GA]
BsaWI 476 TCCGGA [AT]^CCGG[AT]
BseAI 476 TCCGGA T^CCGGA
BsgI 1713 CTGCAC GTGCAG(16/14)
BsiEI 59 CGGTCG CG[GA][CT]^CG
BsmBI 1448 GAGACG CGTCTC(1/5)
BsoBI 1063 CCCGAG C^[CT]CG[GA]G
BspEI 476 TCCGGA T^CCGGA
BspHI 416 TCATGA T^CATGA
BstAPI 440 GCAACGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraI 846 TTTAAA TTT^AAA
EcoNI 46 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
KasI 1798 GGCGCC G^GCGCC
MroI 476 TCCGGA T^CCGGA
MslI 451 CATCACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 1798 GGCGCC GG^CGCC
NcoI 1873 CCATGG C^CATGG
PleI 1605 GACTC GAGTC(4/5)
PmaCI 1837 CACGTG CAC^GTG
PmlI 1837 CACGTG CAC^GTG
PshBI 1468 ATTAAT AT^TAAT
RcaI 416 TCATGA T^CATGA
SacI 1163 GAGCTC GAGCT^C
SapI 1590 GAAGAGC GCTCTTC(1/4)
SfaNI 1287 GATGC GCATC(5/9)
SfoI 1798 GGCGCC GGC^GCC
SpeI 835 ACTAGT A^CTAGT
SspI 1623 AATATT AAT^ATT
SwaI 845 ATTTAAAT ATTT^AAAT
TfiI 229 GAATC G^A[AT]TC
XcmI 1404 CCACCAGTCCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AatII 1450 GACGTC GACGT^C
1825 GACGTC
AccI 407 GTCTAC GT^[AC][GT]AC
831 GTCTAC
AlwNI 17 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
1119 CAGAGCCTG
AspHI 1163 GAGCTC G[AT]GC[AT]^C
1810 GAGCAC
BbsI 561 GAAGAC GAAGAC(2/6)
1480 GAAGAC
BlpI 964 GCTCAGC GC^T[ACGT]AGC
1036 GCTGAGC
BpmI 128 CTGGAG CTGGAG(16/14)
1106 CTGGAG
Bpu1102I 964 GCTCAGC GC^T[ACGT]AGC
1036 GCTGAGC
BpuAI 561 GAAGAC GAAGAC(2/6)
1480 GAAGAC
BsaBI 550 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1015 GATGGCTATC
BsaI 636 GAGACC GGTCTC(1/5)
1109 GAGACC
BseRI 122 CTCCTC GAGGAG(10/8)
887 GAGGAG
BsiHKAI 1163 GAGCTC G[AT]GC[AT]^C
1810 GAGCAC
Bsp1407I 358 TGTACA T^GTACA
1703 TGTACA
BsrBI 119 CCGCTC CCGCTC(-3/-3)
56 GAGCGG
BsrFI 99 GCCGGT [GA]^CCGG[CT]
1190 ACCGGC
BsrGI 358 TGTACA T^GTACA
1703 TGTACA
BstXI 450 CCATCACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1407 CCAGTCCATTGG
CelII 964 GCTCAGC GC^T[ACGT]AGC
1036 GCTGAGC
Cfr10I 99 GCCGGT [GA]^CCGG[CT]
1190 ACCGGC
DrdI 528 GACATGGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1491 GACAGCCAAGTC
EcoT14I 1329 CCTAGG C^C[AT][AT]GG
1873 CCATGG
HaeII 968 AGCGCC [GA]GCGC^[CT]
1798 GGCGCC
HinfI 229 GAATC G^A[ACGT]TC
1605 GACTC
MamI 550 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1015 GATGGCTATC
PpuMI 18 AGGACCT [GA]G^G[AT]CC[CT]
776 AGGACCT
SfcI 426 CTACAG C^T[GA][CT]AG
1116 CTACAG
SspBI 358 TGTACA T^GTACA
1703 TGTACA
StyI 1329 CCTAGG C^C[AT][AT]GG
1873 CCATGG
TaqI 203 TCGA T^CGA
1161 TCGA
Tsp45I 947 GTGAC ^GT[GC]AC
1784 GTGAC
TthHB8I 203 TCGA T^CGA
1161 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI ApoI AvaII BalI BanI
BanII BbvI BciVI BcnI BfaI
BmyI BsaHI BsaJI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI Hin1I HinP1I HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI Tru9I
TseI Tsp509I TspRI XhoII