(Circular) (Six-base) MAPSORT of: pFN21AB5154.seq Check: 1763 from: 1 to: 6589 pFN21AB5154 6589 bp With 182 enzymes: SgfI * February 27, 2009 14:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3459 3834 4399 278 Size: 53 83 186 936 1923 375 565 2468 Fragments arranged by size: 2468 1923 936 565 375 186 83 53 AccI GT'mk_AC Cuts at: 1937 2413 2837 3932 1937 Size: 476 424 1095 4594 Fragments arranged by size: 4594 1095 476 424 Acc65I G'GTAC_C Cuts at: 3910 3910 Size: 6589 AclI AA'CG_TT Cuts at: 1563 4716 5089 1563 Size: 3153 373 3063 Fragments arranged by size: 3153 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2205 3504 3623 4652 6036 6304 1632 Size: 573 1299 119 1029 1384 268 1917 Fragments arranged by size: 1917 1384 1299 1029 573 268 119 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6392 AflIII A'CryG_T Cuts at: 1203 5488 6465 1203 Size: 4285 977 1327 Fragments arranged by size: 4285 1327 977 AgeI A'CCGG_T Cuts at: 5420 5420 Size: 6589 AhdI GACnn_n'nnGTC Cuts at: 5318 5318 Size: 6589 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6589 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6589 AlwNI CAG_nnn'CTG Cuts at: 2027 3129 4332 5904 2027 Size: 1102 1203 1572 2712 Fragments arranged by size: 2712 1572 1203 1102 ApaLI G'TGCA_C Cuts at: 4646 5802 4646 Size: 1156 5433 ApoI r'AATT_y Cuts at: 1493 2762 2848 3368 3542 3898 4124 4276 6385 Size: 1269 86 520 174 356 226 152 2109 Cuts at: 6385 1493 Size: 1697 Fragments arranged by size: 2109 1697 1269 520 356 226 174 152 86 AseI AT'TA_AT Cuts at: 160 1032 3474 5143 160 Size: 872 2442 1669 1606 Fragments arranged by size: 2442 1669 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6589 AvaI C'yCGr_G Cuts at: 1870 1943 3068 3914 1870 Size: 73 1125 846 4545 Fragments arranged by size: 4545 1125 846 73 AvrII C'CTAG_G Cuts at: 3334 3334 Size: 6589 BamHI G'GATC_C Cuts at: 3919 4286 3919 Size: 367 6222 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2042 3260 3803 3910 5365 Size: 325 217 627 255 1218 543 107 1455 Cuts at: 5365 618 Size: 1842 Fragments arranged by size: 1842 1455 1218 627 543 325 255 217 107 BanII G_rGCy'C Cuts at: 1391 2169 2306 3172 3908 1391 Size: 778 137 866 736 4072 Fragments arranged by size: 4072 866 778 736 137 BbeI G_GCGC'C Cuts at: 3807 3807 Size: 6589 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2573 3492 928 Size: 971 674 919 4025 Fragments arranged by size: 4025 971 919 674 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4780 4814 1903 Size: 34 2843 34 3678 Fragments arranged by size: 3678 2843 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2266 2284 3765 4481 5691 2266 Size: 18 1481 716 1210 3164 Fragments arranged by size: 3164 1481 1210 716 18 BclI T'GATC_A Cuts at: 1548 1548 Size: 6589 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 5200 136 Size: 107 122 71 631 605 337 3191 1525 Fragments arranged by size: 3191 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 3183 6584 3183 Size: 3401 3188 BlpI GC'TnA_GC Cuts at: 2970 3042 4006 2970 Size: 72 964 5553 Fragments arranged by size: 5553 964 72 Bme1580I G_kGCm'C Cuts at: 1790 4650 5806 1790 Size: 2860 1156 2573 Fragments arranged by size: 2860 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6589 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2163 2626 3169 Size: 416 53 83 399 24 746 463 543 Cuts at: 3169 3326 4320 5278 442 Size: 157 994 958 1753 Fragments arranged by size: 1753 994 958 746 543 463 416 399 157 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6589 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2161 3258 2161 Size: 1097 5492 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2154 3132 5249 1239 Size: 915 978 2117 2579 Fragments arranged by size: 2579 2117 978 915 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4714 5579 5877 4714 Size: 865 298 5426 Fragments arranged by size: 5426 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2635 3108 5252 882 Size: 617 1136 473 2144 2219 Fragments arranged by size: 2219 2144 1136 617 473 BsaAI yAC'GTr Cuts at: 493 1204 3844 4380 493 Size: 711 2640 536 2702 Fragments arranged by size: 2702 2640 711 536 BsaBI GATnn'nnATC Cuts at: 2559 3024 6583 2559 Size: 465 3559 2565 Fragments arranged by size: 3559 2565 465 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3456 3804 3831 4396 Size: 53 83 186 936 1923 348 27 565 Cuts at: 4396 4778 5413 275 Size: 382 635 1451 Fragments arranged by size: 1923 1451 936 635 565 382 348 186 83 53 27 BsaWI w'CCGG_w Cuts at: 2481 5022 5420 5694 5841 6252 2481 Size: 2541 398 274 147 411 2818 Fragments arranged by size: 2818 2541 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3856 3886 1178 Size: 30 165 30 2453 30 3881 Fragments arranged by size: 3881 2453 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2118 2907 1186 Size: 932 789 4868 Fragments arranged by size: 4868 932 789 BseYI C'CCAG_C Cuts at: 2267 3090 3327 3694 3798 5792 6288 2267 Size: 823 237 367 104 1994 496 2568 Fragments arranged by size: 2568 1994 823 496 367 237 104 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3703 1163 Size: 573 1967 4049 Fragments arranged by size: 4049 1967 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2067 4039 4800 5828 1540 Size: 275 186 66 1972 761 1028 2301 Fragments arranged by size: 2301 1972 1028 761 275 186 66 BsiHKAI G_wGCw'C Cuts at: 3172 3819 4650 4735 5806 3172 Size: 647 831 85 1071 3955 Fragments arranged by size: 3955 1071 831 647 85 BsmI GAATG_Cn' Cuts at: 4100 4193 4100 Size: 93 6496 BsmBI CGTCTCn'nnnn_ Cuts at: 3447 3447 Size: 6589 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2169 2306 3172 3819 3908 4650 4735 Size: 399 379 137 866 647 89 742 85 Cuts at: 4735 5806 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 866 742 647 399 379 137 89 85 BspEI T'CCGG_A Cuts at: 2481 6252 2481 Size: 3771 2818 BspHI T'CATG_A Cuts at: 2421 4478 6208 2421 Size: 2057 1730 2802 Fragments arranged by size: 2802 2057 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3944 844 Size: 3100 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2063 2126 4476 1394 Size: 429 240 63 2350 3507 Fragments arranged by size: 3507 2350 429 240 63 BsrDI GCAATG_nn' Cuts at: 65 1248 2357 2440 3506 5084 5258 65 Size: 1183 1109 83 1066 1578 174 1396 Fragments arranged by size: 1578 1396 1183 1109 1066 174 83 BsrFI r'CCGG_y Cuts at: 1700 1810 2104 3195 5233 5420 1700 Size: 110 294 1091 2038 187 2869 Fragments arranged by size: 2869 2038 1091 294 187 110 BsrGI T'GTAC_A Cuts at: 96 2363 3708 96 Size: 2267 1345 2977 Fragments arranged by size: 2977 2267 1345 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6589 BssSI C'ACGA_G Cuts at: 4649 5661 4649 Size: 1012 5577 BstAPI GCAn_nnn'nTGC Cuts at: 794 2451 794 Size: 1657 4932 BstBI TT'CG_AA Cuts at: 5393 5393 Size: 6589 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6589 BstXI CCAn_nnnn'nTGG Cuts at: 2462 3419 2462 Size: 957 5632 BstYI r'GATC_y Cuts at: 1972 2142 2725 2740 3183 3766 3919 4286 4671 Size: 170 583 15 443 583 153 367 385 Cuts at: 4671 4688 6140 6226 6238 6584 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 583 583 443 385 367 346 170 153 86 17 15 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2307 3807 3878 513 Size: 552 939 303 1500 71 3224 Fragments arranged by size: 3224 1500 939 552 303 71 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5850 Fragments arranged by size: 5850 683 56 BtsI GCAGTG_nn' Cuts at: 2833 4105 4899 4919 2833 Size: 1272 794 20 4503 Fragments arranged by size: 4503 1272 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6589 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6554 DraI TTT'AAA Cuts at: 2853 3893 2853 Size: 1040 5549 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2539 3502 5596 809 Size: 507 1223 963 2094 1802 Fragments arranged by size: 2094 1802 1223 963 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2029 2323 2523 Size: 54 1425 50 275 18 199 294 200 Cuts at: 2523 2660 4036 4925 6554 8 Size: 137 1376 889 1629 43 Fragments arranged by size: 1629 1425 1376 889 294 275 200 199 137 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4036 1537 Size: 275 2224 4090 Fragments arranged by size: 4090 2224 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2082 2310 3439 3590 4519 1353 Size: 729 228 1129 151 929 3423 Fragments arranged by size: 3423 1129 929 729 228 151 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3400 5176 5550 5696 387 Size: 1064 138 1811 1776 374 146 1280 Fragments arranged by size: 1811 1776 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3170 3170 Size: 6589 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2154 2205 3132 3504 3623 4652 5249 Size: 393 522 51 927 372 119 1029 597 Cuts at: 5249 6036 6304 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1029 927 787 597 522 393 372 268 119 51 EcoNI CCTnn'n_nnAGG Cuts at: 2055 2055 Size: 6589 EcoO109I rG'GnC_Cy Cuts at: 2024 2782 3237 2024 Size: 758 455 5376 Fragments arranged by size: 5376 758 455 EcoRI G'AATT_C Cuts at: 3898 4276 3898 Size: 378 6211 FspI TGC'GCA Cuts at: 5095 5095 Size: 6589 HaeII r_GCGC'y Cuts at: 1678 2977 3807 5736 1678 Size: 1299 830 1929 2531 Fragments arranged by size: 2531 1929 1299 830 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2127 2159 2219 2251 2278 Size: 32 135 32 555 32 60 32 27 Cuts at: 2278 2310 2390 2422 3856 3888 5230 5262 5304 Size: 32 80 32 1434 32 1342 32 42 Cuts at: 5304 5336 1373 Size: 32 2626 Fragments arranged by size: 2626 1434 1342 555 135 80 60 42 32 32 32 32 32 32 32 32 32 27 HincII GTy'rAC Cuts at: 669 1657 1938 3933 4179 669 Size: 988 281 1995 246 3079 Fragments arranged by size: 3079 1995 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6589 HpaI GTT'AAC Cuts at: 4179 4179 Size: 6589 KasI G'GCGC_C Cuts at: 3803 3803 Size: 6589 KpnI G_GTAC'C Cuts at: 3914 3914 Size: 6589 MfeI C'AATT_G Cuts at: 4188 4188 Size: 6589 MluI A'CGCG_T Cuts at: 6465 6465 Size: 6589 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2180 3190 5703 5887 1305 Size: 875 1010 2513 184 2007 Fragments arranged by size: 2513 2007 1010 875 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2031 2525 2662 10 Size: 54 1425 343 199 494 137 3937 Fragments arranged by size: 3937 1425 494 343 199 137 54 MslI CAynn'nnrTG Cuts at: 518 1166 2460 4547 4906 5065 518 Size: 648 1294 2087 359 159 2042 Fragments arranged by size: 2087 2042 1294 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2114 2880 3308 4005 4682 5830 6075 1606 Size: 508 766 428 697 677 1148 245 2120 Fragments arranged by size: 2120 1148 766 697 677 508 428 245 NarI GG'CG_CC Cuts at: 3804 3804 Size: 6589 NcoI C'CATG_G Cuts at: 513 1065 2004 3878 513 Size: 552 939 1874 3224 Fragments arranged by size: 3224 1874 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6589 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6589 NotI GC'GGCC_GC Cuts at: 4036 4036 Size: 6589 NspI r_CATG'y Cuts at: 1132 1229 3947 5492 1132 Size: 97 2718 1545 2229 Fragments arranged by size: 2718 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6589 PciI A'CATG_T Cuts at: 5488 5488 Size: 6589 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6589 PfoI T'CCnGG_A Cuts at: 2131 3109 2131 Size: 978 5611 PmeI GTTT'AAAC Cuts at: 3893 3893 Size: 6589 PmlI CAC'GTG Cuts at: 3844 3844 Size: 6589 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4658 4690 6171 6203 4658 Size: 32 1481 32 5044 Fragments arranged by size: 5044 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2024 2782 2024 Size: 758 5831 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6589 PsiI TTA'TAA Cuts at: 3636 4159 3636 Size: 523 6066 PstI C_TGCA'G Cuts at: 830 3941 830 Size: 3111 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6589 SacI G_AGCT'C Cuts at: 3172 3172 Size: 6589 SalI G'TCGA_C Cuts at: 1936 3931 1936 Size: 1995 4594 SapI GCTCTTCn'nnn_ Cuts at: 3590 3590 Size: 6589 SbfI CC_TGCA'GG Cuts at: 3941 3941 Size: 6589 ScaI AGT'ACT Cuts at: 4837 6549 4837 Size: 1712 4877 SfcI C'TryA_G Cuts at: 826 1045 2431 3121 3937 5072 5753 5944 826 Size: 219 1386 690 816 1135 681 191 1471 Fragments arranged by size: 1471 1386 1135 816 690 681 219 191 SfoI GGC'GCC Cuts at: 3805 3805 Size: 6589 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6589 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6589 SmaI CCC'GGG Cuts at: 1872 3916 1872 Size: 2044 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4693 5594 5856 820 Size: 197 926 2750 901 262 1553 Fragments arranged by size: 2750 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4380 493 Size: 3887 2702 SpeI A'CTAG_T Cuts at: 152 2840 152 Size: 2688 3901 SphI G_CATG'C Cuts at: 1132 3947 1132 Size: 2815 3774 SspI AAT'ATT Cuts at: 5 52 3630 4513 5 Size: 47 3578 883 2081 Fragments arranged by size: 3578 2081 883 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6589 StyI C'CwwG_G Cuts at: 513 1065 2004 3334 3878 4028 513 Size: 552 939 1330 544 150 3074 Fragments arranged by size: 3074 1330 939 552 544 150 SwaI ATTT'AAAT Cuts at: 2853 2853 Size: 6589 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2324 2736 4616 4633 4786 4971 5416 Size: 1255 744 412 1880 17 153 185 445 Cuts at: 5416 325 Size: 1498 Fragments arranged by size: 1880 1498 1255 744 445 412 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2363 3708 4835 Size: 274 80 33 51 1443 386 1345 1127 Cuts at: 4835 6547 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1345 1127 386 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6549 6581 6549 Size: 32 6557 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6589 XbaI T'CTAG_A Cuts at: 3925 3925 Size: 6589 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3416 3416 Size: 6589 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6589 XmaI C'CCGG_G Cuts at: 1870 3914 1870 Size: 2044 4545 XmnI GAAnn'nnTTC Cuts at: 4718 4718 Size: 6589 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3457 3832 4397 276 Size: 53 83 186 936 1923 375 565 2468 Fragments arranged by size: 2468 1923 936 565 375 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SrfI