Restriction map for
pFN21AB5154
(Linear) (Six-base) MAPSORT of: KIBB5154.seq Check: 6865 from: 1 to: 1884
KIBB5154.orf
With 182 enzymes: SgfI *
February 28, 2007 11:27 ..
AatII G_ACGT'C
Cuts at: 0 1454 1829 1884
Size: 1454 375 55
Fragments arranged by size:
1454 375 55
AccI GT'mk_AC
Cuts at: 0 408 832 1884
Size: 408 424 1052
Fragments arranged by size:
1052 424 408
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 200 1499 1618 1884
Size: 200 1299 119 266
Fragments arranged by size:
1299 266 200 119
AlwNI CAG_nnn'CTG
Cuts at: 0 22 1124 1884
Size: 22 1102 760
Fragments arranged by size:
1102 760 22
ApoI r'AATT_y
Cuts at: 0 757 843 1363 1537 1884
Size: 757 86 520 174 347
Fragments arranged by size:
757 520 347 174 86
AseI AT'TA_AT
Cuts at: 0 1469 1884
Size: 1469 415
AvaI C'yCGr_G
Cuts at: 0 1063 1884
Size: 1063 821
AvrII C'CTAG_G
Cuts at: 0 1329 1884
Size: 1329 555
BanI G'GyrC_C
Cuts at: 0 37 1255 1798 1884
Size: 37 1218 543 86
Fragments arranged by size:
1218 543 86 37
BanII G_rGCy'C
Cuts at: 0 164 301 1167 1884
Size: 164 137 866 717
Fragments arranged by size:
866 717 164 137
BbeI G_GCGC'C
Cuts at: 0 1802 1884
Size: 1802 82
BbsI GAAGACnn'nnnn_
Cuts at: 0 568 1487 1884
Size: 568 919 397
Fragments arranged by size:
919 568 397
BciVI GTATCCnnnnn_n'
Cuts at: 0 261 279 1760 1884
Size: 261 18 1481 124
Fragments arranged by size:
1481 261 124 18
BglII A'GATC_T
Cuts at: 0 1178 1884
Size: 1178 706
BlpI GC'TnA_GC
Cuts at: 0 965 1037 1884
Size: 965 72 847
Fragments arranged by size:
965 847 72
BmrI ACTGGGnnnn_n'
Cuts at: 0 158 621 1164 1321 1884
Size: 158 463 543 157 563
Fragments arranged by size:
563 543 463 158 157
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 156 1253 1884
Size: 156 1097 631
Fragments arranged by size:
1097 631 156
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 149 1127 1884
Size: 149 978 757
Fragments arranged by size:
978 757 149
BsaI GGTCTCn'nnnn_
Cuts at: 0 630 1103 1884
Size: 630 473 781
Fragments arranged by size:
781 630 473
BsaAI yAC'GTr
Cuts at: 0 1839 1884
Size: 1839 45
BsaBI GATnn'nnATC
Cuts at: 0 554 1019 1884
Size: 554 465 865
Fragments arranged by size:
865 554 465
BsaHI Gr'CG_yC
Cuts at: 0 1451 1799 1826 1884
Size: 1451 348 27 58
Fragments arranged by size:
1451 348 58 27
BsaWI w'CCGG_w
Cuts at: 0 476 1884
Size: 476 1408
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1851 1881 1884
Size: 1851 30 3
Fragments arranged by size:
1851 30 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 113 902 1884
Size: 113 789 982
Fragments arranged by size:
982 789 113
BseYI C'CCAG_C
Cuts at: 0 262 1085 1322 1689 1793 1884
Size: 262 823 237 367 104 91
Fragments arranged by size:
823 367 262 237 104 91
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1698 1884
Size: 1698 186
BsiEI CG_ry'CG
Cuts at: 0 62 1884
Size: 62 1822
BsiHKAI G_wGCw'C
Cuts at: 0 1167 1814 1884
Size: 1167 647 70
Fragments arranged by size:
1167 647 70
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1442 1884
Size: 1442 442
Bsp1286I G_dGCh'C
Cuts at: 0 164 301 1167 1814 1884
Size: 164 137 866 647 70
Fragments arranged by size:
866 647 164 137 70
BspEI T'CCGG_A
Cuts at: 0 476 1884
Size: 476 1408
BspHI T'CATG_A
Cuts at: 0 416 1884
Size: 416 1468
BsrBI CCG'CTC
Cuts at: 0 58 121 1884
Size: 58 63 1763
Fragments arranged by size:
1763 63 58
BsrDI GCAATG_nn'
Cuts at: 0 352 435 1501 1884
Size: 352 83 1066 383
Fragments arranged by size:
1066 383 352 83
BsrFI r'CCGG_y
Cuts at: 0 99 1190 1884
Size: 99 1091 694
Fragments arranged by size:
1091 694 99
BsrGI T'GTAC_A
Cuts at: 0 358 1703 1884
Size: 358 1345 181
Fragments arranged by size:
1345 358 181
BstAPI GCAn_nnn'nTGC
Cuts at: 0 446 1884
Size: 446 1438
BstXI CCAn_nnnn'nTGG
Cuts at: 0 457 1414 1884
Size: 457 957 470
Fragments arranged by size:
957 470 457
BstYI r'GATC_y
Cuts at: 0 137 720 735 1178 1761 1884
Size: 137 583 15 443 583 123
Fragments arranged by size:
583 583 443 137 123 15
BtgI C'CryG_G
Cuts at: 0 302 1802 1873 1884
Size: 302 1500 71 11
Fragments arranged by size:
1500 302 71 11
BtsI GCAGTG_nn'
Cuts at: 0 828 1884
Size: 828 1056
DraI TTT'AAA
Cuts at: 0 848 1884
Size: 848 1036
DrdI GACnn_nn'nnGTC
Cuts at: 0 534 1497 1884
Size: 534 963 387
Fragments arranged by size:
963 534 387
EaeI y'GGCC_r
Cuts at: 0 24 318 518 655 1884
Size: 24 294 200 137 1229
Fragments arranged by size:
1229 294 200 137 24
EarI CTCTTCn'nnn_
Cuts at: 0 77 305 1434 1585 1884
Size: 77 228 1129 151 299
Fragments arranged by size:
1129 299 228 151 77
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1395 1884
Size: 1395 489
EcoICRI GAG'CTC
Cuts at: 0 1165 1884
Size: 1165 719
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 149 200 1127 1499 1618 1884
Size: 149 51 927 372 119 266
Fragments arranged by size:
927 372 266 149 119 51
EcoNI CCTnn'n_nnAGG
Cuts at: 0 50 1884
Size: 50 1834
EcoO109I rG'GnC_Cy
Cuts at: 0 19 777 1232 1884
Size: 19 758 455 652
Fragments arranged by size:
758 652 455 19
HaeII r_GCGC'y
Cuts at: 0 972 1802 1884
Size: 972 830 82
Fragments arranged by size:
972 830 82
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 122 154 214 246 273 305 385 417
Size: 122 32 60 32 27 32 80 32
Cuts at: 417 1851 1883 1884
Size: 1434 32 1
Fragments arranged by size:
1434 122 80 60 32 32 32 32
32 27 1
KasI G'GCGC_C
Cuts at: 0 1798 1884
Size: 1798 86
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 175 1185 1884
Size: 175 1010 699
Fragments arranged by size:
1010 699 175
MscI TGG'CCA
Cuts at: 0 26 520 657 1884
Size: 26 494 137 1227
Fragments arranged by size:
1227 494 137 26
MslI CAynn'nnrTG
Cuts at: 0 455 1884
Size: 455 1429
MspA1I CmG'CkG
Cuts at: 0 109 875 1303 1884
Size: 109 766 428 581
Fragments arranged by size:
766 581 428 109
NarI GG'CG_CC
Cuts at: 0 1799 1884
Size: 1799 85
NcoI C'CATG_G
Cuts at: 0 1873 1884
Size: 1873 11
PfoI T'CCnGG_A
Cuts at: 0 126 1104 1884
Size: 126 978 780
Fragments arranged by size:
978 780 126
PmlI CAC'GTG
Cuts at: 0 1839 1884
Size: 1839 45
PpuMI rG'GwC_Cy
Cuts at: 0 19 777 1884
Size: 19 758 1107
Fragments arranged by size:
1107 758 19
PsiI TTA'TAA
Cuts at: 0 1631 1884
Size: 1631 253
SacI G_AGCT'C
Cuts at: 0 1167 1884
Size: 1167 717
SapI GCTCTTCn'nnn_
Cuts at: 0 1585 1884
Size: 1585 299
SfcI C'TryA_G
Cuts at: 0 426 1116 1884
Size: 426 690 768
Fragments arranged by size:
768 690 426
SfoI GGC'GCC
Cuts at: 0 1800 1884
Size: 1800 84
SpeI A'CTAG_T
Cuts at: 0 835 1884
Size: 835 1049
SspI AAT'ATT
Cuts at: 0 1625 1884
Size: 1625 259
StyI C'CwwG_G
Cuts at: 0 1329 1873 1884
Size: 1329 544 11
Fragments arranged by size:
1329 544 11
SwaI ATTT'AAAT
Cuts at: 0 848 1884
Size: 848 1036
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 319 731 1884
Size: 319 412 1153
Fragments arranged by size:
1153 412 319
TatI w'GTAC_w
Cuts at: 0 358 1703 1884
Size: 358 1345 181
Fragments arranged by size:
1345 358 181
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1411 1884
Size: 1411 473
ZraI GAC'GTC
Cuts at: 0 1452 1827 1884
Size: 1452 375 57
Fragments arranged by size:
1452 375 57
Enzymes that do cut:
AatII AccI AcuI AlwNI ApoI AseI AvaI AvrII
BanI BanII BbeI BbsI BciVI BglII BlpI BmrI
BplI BpmI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BsrBI BsrDI BsrFI BsrGI BstAPI BstXI BstYI
BtgI BtsI DraI DrdI EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I HaeII Hin4I KasI MmeI MscI
MslI MspA1I NarI NcoI PfoI PmlI PpuMI PsiI
SacI SapI SfcI SfoI SpeI SspI StyI SwaI
TaqII TatI XcmI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AsiSI BaeI
BamHI BbvCI BcgI BclI BfrBI BglI Bme1580I BmgBI
BmtI Bpu10I BpuEI BsiWI BsmI BspMI BssHII BssSI
BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraIII
EagI EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KpnI MfeI MluI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PflMI PmeI PpiI PshAI PspOMI PspXI PsrI PstI
PvuI PvuII RsrII SacII SalI SanDI SbfI ScaI
SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI SphI
SrfI StuI TstI Tth111I XbaI XhoI XmaI XmnI