(Circular) (Six-base) MAPSORT of: pFN21AB5140.seq Check: 848 from: 1 to: 5884 pFN21AB5140 5884 bp With 182 enzymes: SgfI * February 27, 2009 14:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3694 278 Size: 53 83 186 936 2158 2468 Fragments arranged by size: 2468 2158 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2889 3227 1937 Size: 952 338 4594 Fragments arranged by size: 4594 952 338 Acc65I G'GTAC_C Cuts at: 3205 3205 Size: 5884 AclI AA'CG_TT Cuts at: 1563 4011 4384 1563 Size: 2448 373 3063 Fragments arranged by size: 3063 2448 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3947 5331 5599 1632 Size: 2315 1384 268 1917 Fragments arranged by size: 2315 1917 1384 268 AfeI AGC'GCT Cuts at: 2072 2072 Size: 5884 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5687 AflIII A'CryG_T Cuts at: 1203 4783 5760 1203 Size: 3580 977 1327 Fragments arranged by size: 3580 1327 977 AgeI A'CCGG_T Cuts at: 4715 4715 Size: 5884 AhdI GACnn_n'nnGTC Cuts at: 4613 4613 Size: 5884 AleI CACnn'nnGTG Cuts at: 1166 2159 2921 2998 1166 Size: 993 762 77 4052 Fragments arranged by size: 4052 993 762 77 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2432 1184 Size: 1248 4636 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2645 2677 2645 Size: 32 5852 AlwNI CAG_nnn'CTG Cuts at: 2650 3026 3627 5199 2650 Size: 376 601 1572 3335 Fragments arranged by size: 3335 1572 601 376 ApaLI G'TGCA_C Cuts at: 3941 5097 3941 Size: 1156 4728 ApoI r'AATT_y Cuts at: 1493 3193 3419 3571 5680 1493 Size: 1700 226 152 2109 1697 Fragments arranged by size: 2109 1700 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4438 160 Size: 872 3406 1606 Fragments arranged by size: 3406 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5884 AvaI C'yCGr_G Cuts at: 1870 1943 2033 2402 2798 2831 3009 3209 1870 Size: 73 90 369 396 33 178 200 4545 Fragments arranged by size: 4545 396 369 200 178 90 73 33 BamHI G'GATC_C Cuts at: 3214 3581 3214 Size: 367 5517 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2055 2175 2231 2376 2556 Size: 325 217 627 268 120 56 145 180 Cuts at: 2556 2986 3205 4660 618 Size: 430 219 1455 1842 Fragments arranged by size: 1842 1455 627 430 325 268 219 217 180 145 120 56 BanII G_rGCy'C Cuts at: 1391 2444 2879 3203 1391 Size: 1053 435 324 4072 Fragments arranged by size: 4072 1053 435 324 BbeI G_GCGC'C Cuts at: 2059 2179 2235 2560 2059 Size: 120 56 325 5383 Fragments arranged by size: 5383 325 120 56 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2512 928 Size: 971 613 4300 Fragments arranged by size: 4300 971 613 BbvCI CC'TCA_GC Cuts at: 2180 2687 2180 Size: 507 5377 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4075 4109 1903 Size: 34 2138 34 3678 Fragments arranged by size: 3678 2138 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3776 4986 3776 Size: 1210 4674 BclI T'GATC_A Cuts at: 1548 1548 Size: 5884 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2053 2406 4495 Size: 107 122 71 631 605 381 353 2089 Cuts at: 4495 136 Size: 1525 Fragments arranged by size: 2089 1525 631 605 381 353 122 107 71 BglII A'GATC_T Cuts at: 2484 5879 2484 Size: 3395 2489 BlpI GC'TnA_GC Cuts at: 3301 3301 Size: 5884 Bme1580I G_kGCm'C Cuts at: 1790 2379 2402 2797 2991 3945 5101 1790 Size: 589 23 395 194 954 1156 2573 Fragments arranged by size: 2573 1156 954 589 395 194 23 BmgBI CAC'GTC Cuts at: 1318 2919 1318 Size: 1601 4283 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3615 4573 442 Size: 416 53 83 399 24 2198 958 1753 Fragments arranged by size: 2198 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5884 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2291 2300 2672 2954 4544 1239 Size: 1052 9 372 282 1590 2579 Fragments arranged by size: 2579 1590 1052 372 282 9 Bpu10I CC'TnA_GC Cuts at: 2180 2687 2180 Size: 507 5377 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4009 4874 5172 4009 Size: 865 298 4721 Fragments arranged by size: 4721 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2345 2351 4547 882 Size: 617 846 6 2196 2219 Fragments arranged by size: 2219 2196 846 617 6 BsaAI yAC'GTr Cuts at: 493 1204 2077 3675 493 Size: 711 873 1598 2702 Fragments arranged by size: 2702 1598 873 711 BsaBI GATnn'nnATC Cuts at: 3045 5878 3045 Size: 2833 3051 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2056 2176 2232 2297 Size: 53 83 186 936 523 120 56 65 Cuts at: 2297 2557 2760 2783 3691 4073 4708 275 Size: 260 203 23 908 382 635 1451 Fragments arranged by size: 1451 936 908 635 523 382 260 203 186 120 83 65 56 53 23 BsaWI w'CCGG_w Cuts at: 4317 4715 4989 5136 5547 4317 Size: 398 274 147 411 4654 Fragments arranged by size: 4654 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2576 2606 2645 2675 1178 Size: 30 165 30 1173 30 39 30 4387 Fragments arranged by size: 4387 1173 165 39 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2057 2515 2584 3104 3107 1186 Size: 871 458 69 520 3 3963 Fragments arranged by size: 3963 871 520 458 69 3 BseYI C'CCAG_C Cuts at: 3065 3125 5087 5583 3065 Size: 60 1962 496 3366 Fragments arranged by size: 3366 1962 496 60 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2123 2657 3022 1163 Size: 573 387 534 365 4025 Fragments arranged by size: 4025 573 534 387 365 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2065 2260 2716 3334 4095 5123 Size: 275 186 64 195 456 618 761 1028 Cuts at: 5123 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 618 456 275 195 186 64 BsiHKAI G_wGCw'C Cuts at: 2277 2879 3010 3945 4030 5101 2277 Size: 602 131 935 85 1071 3060 Fragments arranged by size: 3060 1071 935 602 131 85 BsmI GAATG_Cn' Cuts at: 3395 3488 3395 Size: 93 5791 BsmBI CGTCTCn'nnnn_ Cuts at: 2703 2734 2703 Size: 31 5853 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2277 2379 2402 2444 2797 2879 2991 Size: 399 487 102 23 42 353 82 112 Cuts at: 2991 3010 3203 3945 4030 5101 1391 Size: 19 193 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 487 399 353 193 112 102 85 82 42 23 19 BspEI T'CCGG_A Cuts at: 5547 5547 Size: 5884 BspHI T'CATG_A Cuts at: 3773 5503 3773 Size: 1730 4154 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2175 3239 844 Size: 1331 1064 3489 Fragments arranged by size: 3489 1331 1064 BsrBI CCG'CTC Cuts at: 1394 1823 2637 3771 1394 Size: 429 814 1134 3507 Fragments arranged by size: 3507 1134 814 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4379 4553 65 Size: 1183 3131 174 1396 Fragments arranged by size: 3131 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2091 3069 4528 4715 1700 Size: 110 281 978 1459 187 2869 Fragments arranged by size: 2869 1459 978 281 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5884 BssHII G'CGCG_C Cuts at: 1925 2503 1925 Size: 578 5306 BssSI C'ACGA_G Cuts at: 2750 2972 3944 4956 2750 Size: 222 972 1012 3678 Fragments arranged by size: 3678 1012 972 222 BstAPI GCAn_nnn'nTGC Cuts at: 794 3026 794 Size: 2232 3652 BstBI TT'CG_AA Cuts at: 4688 4688 Size: 5884 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5884 BstXI CCAn_nnnn'nTGG Cuts at: 2161 3073 2161 Size: 912 4972 BstYI r'GATC_y Cuts at: 1972 2484 3214 3581 3966 3983 5435 5521 5533 Size: 512 730 367 385 17 1452 86 12 Cuts at: 5533 5879 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 730 512 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2051 2059 2260 2445 2535 2850 Size: 552 939 47 8 201 185 90 315 Cuts at: 2850 513 Size: 3547 Fragments arranged by size: 3547 939 552 315 201 185 90 47 8 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5145 Fragments arranged by size: 5145 683 56 BtsI GCAGTG_nn' Cuts at: 2177 3010 3400 4194 4214 2177 Size: 833 390 794 20 3847 Fragments arranged by size: 3847 833 794 390 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5884 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5849 DraI TTT'AAA Cuts at: 3188 3188 Size: 5884 DraIII CAC_nnn'GTG Cuts at: 2292 2536 2605 2292 Size: 244 69 5571 Fragments arranged by size: 5571 244 69 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4891 809 Size: 507 3575 1802 Fragments arranged by size: 3575 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2062 2089 2257 Size: 54 1425 50 275 18 232 27 168 Cuts at: 2257 2328 3331 4220 5849 8 Size: 71 1003 889 1629 43 Fragments arranged by size: 1629 1425 1003 889 275 232 168 71 54 50 43 27 18 EagI C'GGCC_G Cuts at: 1537 1812 2062 2257 3331 1537 Size: 275 250 195 1074 4090 Fragments arranged by size: 4090 1074 275 250 195 EarI CTCTTCn'nnn_ Cuts at: 1353 2228 2345 2726 3814 1353 Size: 875 117 381 1088 3423 Fragments arranged by size: 3423 1088 875 381 117 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4471 4845 4991 387 Size: 1064 138 2882 374 146 1280 Fragments arranged by size: 2882 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2877 2877 Size: 5884 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2291 2300 2672 2954 3947 4544 5331 Size: 393 659 9 372 282 993 597 787 Cuts at: 5331 5599 1239 Size: 268 1524 Fragments arranged by size: 1524 993 787 659 597 393 372 282 268 9 EcoRI G'AATT_C Cuts at: 3193 3571 3193 Size: 378 5506 FspI TGC'GCA Cuts at: 4390 4390 Size: 5884 HaeII r_GCGC'y Cuts at: 1678 2059 2074 2179 2235 2560 5031 1678 Size: 381 15 105 56 325 2471 2531 Fragments arranged by size: 2531 2471 381 325 105 56 15 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2576 2608 3158 3190 4525 Size: 32 135 32 1004 32 550 32 1335 Cuts at: 4525 4557 4599 4631 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1335 1004 550 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2308 3228 3474 669 Size: 988 281 370 920 246 3079 Fragments arranged by size: 3079 988 920 370 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5884 HpaI GTT'AAC Cuts at: 3474 3474 Size: 5884 KasI G'GCGC_C Cuts at: 2055 2175 2231 2556 2055 Size: 120 56 325 5383 Fragments arranged by size: 5383 325 120 56 KpnI G_GTAC'C Cuts at: 3209 3209 Size: 5884 MfeI C'AATT_G Cuts at: 3483 3483 Size: 5884 MluI A'CGCG_T Cuts at: 5760 5760 Size: 5884 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4998 5182 1305 Size: 3693 184 2007 Fragments arranged by size: 3693 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4062 Fragments arranged by size: 4062 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2159 2855 2921 2998 3842 4201 4360 Size: 648 993 696 66 77 844 359 159 Cuts at: 4360 518 Size: 2042 Fragments arranged by size: 2042 993 844 696 648 359 159 77 66 MspA1I CmG'CkG Cuts at: 1606 2053 2061 2262 2671 2737 3300 3977 5125 Size: 447 8 201 409 66 563 677 1148 Cuts at: 5125 5370 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 563 447 409 245 201 66 8 NaeI GCC'GGC Cuts at: 3071 3071 Size: 5884 NarI GG'CG_CC Cuts at: 2056 2176 2232 2557 2056 Size: 120 56 325 5383 Fragments arranged by size: 5383 325 120 56 NcoI C'CATG_G Cuts at: 513 1065 2004 2445 513 Size: 552 939 441 3952 Fragments arranged by size: 3952 939 552 441 NdeI CA'TA_TG Cuts at: 387 387 Size: 5884 NgoMIV G'CCGG_C Cuts at: 3069 3069 Size: 5884 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5884 NotI GC'GGCC_GC Cuts at: 2062 3331 2062 Size: 1269 4615 NspI r_CATG'y Cuts at: 1132 1229 2476 2865 3242 4787 1132 Size: 97 1247 389 377 1545 2229 Fragments arranged by size: 2229 1545 1247 389 377 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5884 PciI A'CATG_T Cuts at: 4783 4783 Size: 5884 PflMI CCAn_nnn'nTGG Cuts at: 1108 2241 2650 1108 Size: 1133 409 4342 Fragments arranged by size: 4342 1133 409 PmeI GTTT'AAAC Cuts at: 3188 3188 Size: 5884 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2645 2677 3953 3985 5466 5498 2645 Size: 32 1276 32 1481 32 3031 Fragments arranged by size: 3031 1481 1276 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5884 PsiI TTA'TAA Cuts at: 3454 3454 Size: 5884 PspXI vC'TCGA_Gb Cuts at: 2831 2831 Size: 5884 PstI C_TGCA'G Cuts at: 830 2172 2430 3236 830 Size: 1342 258 806 3478 Fragments arranged by size: 3478 1342 806 258 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5884 SacI G_AGCT'C Cuts at: 2879 2879 Size: 5884 SacII CC_GC'GG Cuts at: 2054 2062 2263 2054 Size: 8 201 5675 Fragments arranged by size: 5675 201 8 SalI G'TCGA_C Cuts at: 1936 3226 1936 Size: 1290 4594 SapI GCTCTTCn'nnn_ Cuts at: 2228 2228 Size: 5884 SbfI CC_TGCA'GG Cuts at: 3236 3236 Size: 5884 ScaI AGT'ACT Cuts at: 4132 5844 4132 Size: 1712 4172 SexAI A'CCwGG_T Cuts at: 2883 2883 Size: 5884 SfcI C'TryA_G Cuts at: 826 1045 2168 2426 3232 4367 5048 5239 826 Size: 219 1123 258 806 1135 681 191 1471 Fragments arranged by size: 1471 1135 1123 806 681 258 219 191 SfoI GGC'GCC Cuts at: 2057 2177 2233 2558 2057 Size: 120 56 325 5383 Fragments arranged by size: 5383 325 120 56 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5884 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5884 SmaI CCC'GGG Cuts at: 1872 2035 3211 1872 Size: 163 1176 4545 Fragments arranged by size: 4545 1176 163 SmlI C'TyrA_G Cuts at: 820 1017 1943 2798 2831 3009 3988 4889 5151 Size: 197 926 855 33 178 979 901 262 Cuts at: 5151 820 Size: 1553 Fragments arranged by size: 1553 979 926 901 855 262 197 178 33 SnaBI TAC'GTA Cuts at: 493 3675 493 Size: 3182 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5884 SphI G_CATG'C Cuts at: 1132 3242 1132 Size: 2110 3774 SrfI GCCC'GGGC Cuts at: 2035 2035 Size: 5884 SspI AAT'ATT Cuts at: 5 52 3808 5 Size: 47 3756 2081 Fragments arranged by size: 3756 2081 47 StuI AGG'CCT Cuts at: 1517 2612 1517 Size: 1095 4789 StyI C'CwwG_G Cuts at: 513 1065 2004 2445 3177 3323 513 Size: 552 939 441 732 146 3074 Fragments arranged by size: 3074 939 732 552 441 146 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2369 2696 3911 3928 4081 4266 4711 Size: 1255 789 327 1215 17 153 185 445 Cuts at: 4711 325 Size: 1498 Fragments arranged by size: 1498 1255 1215 789 445 327 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4130 5842 96 Size: 274 80 33 51 1443 2153 1712 138 Fragments arranged by size: 2153 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2078 2110 2156 2188 2276 2308 5844 5876 2078 Size: 32 46 32 88 32 3536 32 2086 Fragments arranged by size: 3536 2086 88 46 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5884 XbaI T'CTAG_A Cuts at: 3220 3220 Size: 5884 XhoI C'TCGA_G Cuts at: 1943 2798 2831 3009 1943 Size: 855 33 178 4818 Fragments arranged by size: 4818 855 178 33 XmaI C'CCGG_G Cuts at: 1870 2033 3209 1870 Size: 163 1176 4545 Fragments arranged by size: 4545 1176 163 XmnI GAAnn'nnTTC Cuts at: 2456 4013 2456 Size: 1557 4327 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3692 276 Size: 53 83 186 936 2158 2468 Fragments arranged by size: 2468 2158 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI PstI PvuI SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I Bsu36I EcoNI EcoO109I EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PsrI PvuII RsrII SanDI SfiI SwaI XcmI