Restriction map for
pFN21AB5122
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# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI Asp700I
AspEI AspI AvaI AviII AvrII
BalI BamHI BanII BbeI BbrPI
BbsI BbvCI BciVI BclI BcnI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiWI
BsmBI BsoBI BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BssHII
BssSI BstBI BstUI BstXI CelII
CfoI Cfr10I ClaI CpoI DraIII
DsaI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoNI EcoRI
EcoRV FbaI FseI FspI HaeII
HapII HhaI Hin1I HinP1I HpaI
HpaII KasI Ksp632I KspI MaeII
MboII MluI MluNI MroI MscI
MspI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NotI NruI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RcaI RsrII SacI
SacII SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I StuI
SwaI Tth111I XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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Acc65I 208 GGTACC G^GTACC
AlwI 364 GGATC GGATC(4/5)
AocI 782 CCTTAGG CC^T[ACGT]AGG
AseI 196 ATTAAT AT^TAAT
AsnI 196 ATTAAT AT^TAAT
Asp718I 208 GGTACC G^GTACC
BcgI 1291 CGAGGGATATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BseRI 400 GAGGAG GAGGAG(10/8)
BspMI 1361 GCAGGT ACCTGC(4/8)
Bst1107I 641 GTATAC GTA^TAC
BstEII 766 GGTAACC G^GT[ACGT]ACC
BstPI 766 GGTAACC G^GT[ACGT]ACC
BstYI 364 GGATCT [GA]^GATC[CT]
BstZ17I 641 GTATAC GTA^TAC
Bsu36I 782 CCTTAGG CC^T[ACGT]AGG
DraI 1116 TTTAAA TTT^AAA
DrdI 1141 GACAATTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 782 CCTTAGG CC^T[ACGT]AGG
EcoO65I 766 GGTAACC G^GT[ACGT]ACC
EcoT14I 1035 CCAAGG C^C[AT][AT]GG
EcoT22I 1299 ATGCAT ATGCA^T
HgaI 880 GACGC GACGC(5/10)
HphI 892 GGTGA GGTGA(8/7)
KpnI 208 GGTACC GGTAC^C
MfeI 1143 CAATTG C^AATTG
MflI 364 GGATCT [GA]^GATC[CT]
MunI 1143 CAATTG C^AATTG
NheI 775 GCTAGC G^CTAGC
NsiI 1299 ATGCAT ATGCA^T
PflMI 746 CCAGAATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1070 GGGACCC [GA]G^G[AT]CC[CT]
PshBI 196 ATTAAT AT^TAAT
SalI 224 GTCGAC G^TCGAC
ScaI 627 AGTACT AGT^ACT
SspI 1455 AATATT AAT^ATT
StyI 1035 CCAAGG C^C[AT][AT]GG
TaqI 225 TCGA T^CGA
Tsp45I 682 GTCAC ^GT[GC]AC
TthHB8I 225 TCGA T^CGA
Van91I 746 CCAGAATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 690 TCTAGA T^CTAGA
XhoII 364 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AcsI 834 AAATTT [GA]^AATT[CT]
1178 AAATTT
ApoI 834 AAATTT [GA]^AATT[CT]
1178 AAATTT
AspHI 410 GAGCAC G[AT]GC[AT]^C
1421 GTGCTC
AvaII 1071 GGACC G^G[AT]CC
1207 GGACC
BanI 121 GGTGCC G^G[CT][GA]CC
208 GGTACC
BpmI 563 CTGGAG CTGGAG(16/14)
851 CTGGAG
BsaBI 147 GATTTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1296 GATATGCATC
BsiHKAI 410 GAGCAC G[AT]GC[AT]^C
1421 GTGCTC
BsiYI 731 CCTATGATGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
746 CCAGAATATGG
BslI 731 CCTATGATGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
746 CCAGAATATGG
BsmI 1473 GAATGC GAATGC(1/-1)
989 GCATTC
Bsp1407I 1334 TGTACA T^GTACA
1395 TGTACA
BsrGI 1334 TGTACA T^GTACA
1395 TGTACA
BssKI 527 CCTGG ^CC[ACGT]GG
1210 CCAGG
BstAPI 322 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
325 GCAGCTGCTGC
BstNI 527 CCTGG CC^[AT]GG
1210 CCAGG
DpnII 365 GATC ^GATC
544 GATC
DraII 798 AGGCCCT [GA]G^G[ACGT]CC[CT]
1070 GGGACCC
EcoO109I 798 AGGCCCT [GA]G^G[ACGT]CC[CT]
1070 GGGACCC
EcoRII 527 CCTGG ^CC[AT]GG
1210 CCAGG
FauI 136 CCCGC CCCGC(4/6)
495 GCGGG
HincII 22 GTCAAC GT[CT]^[GA]AC
224 GTCGAC
HindII 22 GTCAAC GT[CT]^[GA]AC
224 GTCGAC
HindIII 290 AAGCTT A^AGCTT
304 AAGCTT
MaeIII 682 GTCAC ^GT[ACGT]AC
767 GTAAC
MamI 147 GATTTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1296 GATATGCATC
MboI 365 GATC ^GATC
544 GATC
MvaI 527 CCTGG CC^[AT]GG
1210 CCAGG
NdeII 365 GATC ^GATC
544 GATC
PstI 332 CTGCAG CTGCA^G
347 CTGCAG
Sau3AI 365 GATC ^GATC
544 GATC
ScrFI 527 CCTGG CC^[ACGT]GG
1210 CCAGG
SspBI 1334 TGTACA T^GTACA
1395 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AccI AciI AfaI AluI AlwNI
BbvI BfaI BmyI BsaJI BsmAI
BsmFI Bsp1286I BsrI BstF5I Cac8I
Cfr13I DdeI Fnu4HI FokI HaeIII
HinfI ItaI MaeI MnlI MseI
MslI MspA1I MwoI NlaIII NlaIV
PleI PvuII RsaI Sau96I SfaNI
SfcI TfiI Tru9I TseI Tsp509I
TspRI