(Circular) (Six-base) MAPSORT of: pFN21AB5122.seq Check: 1031 from: 1 to: 6247 pFN21AB5122 6247 bp With 182 enzymes: SgfI * February 27, 2009 14:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4057 278 Size: 53 83 186 936 2521 2468 Fragments arranged by size: 2521 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2173 2230 2647 2862 3291 3590 1937 Size: 236 57 417 215 429 299 4594 Fragments arranged by size: 4594 429 417 299 236 215 57 Acc65I G'GTAC_C Cuts at: 2213 3568 2213 Size: 1355 4892 AclI AA'CG_TT Cuts at: 1563 4374 4747 1563 Size: 2811 373 3063 Fragments arranged by size: 3063 2811 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2535 4310 5694 5962 1632 Size: 903 1775 1384 268 1917 Fragments arranged by size: 1917 1775 1384 903 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6050 AflIII A'CryG_T Cuts at: 1203 5146 6123 1203 Size: 3943 977 1327 Fragments arranged by size: 3943 1327 977 AgeI A'CCGG_T Cuts at: 5078 5078 Size: 6247 AhdI GACnn_n'nnGTC Cuts at: 4976 4976 Size: 6247 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6247 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3441 1184 Size: 2257 3990 AlwNI CAG_nnn'CTG Cuts at: 2333 2336 2567 3990 5562 2333 Size: 3 231 1423 1572 3018 Fragments arranged by size: 3018 1572 1423 231 3 ApaLI G'TGCA_C Cuts at: 4304 5460 4304 Size: 1156 5091 ApoI r'AATT_y Cuts at: 1493 2839 3183 3556 3782 3934 6043 1493 Size: 1346 344 373 226 152 2109 1697 Fragments arranged by size: 2109 1697 1346 373 344 226 152 AseI AT'TA_AT Cuts at: 160 1032 2202 4801 160 Size: 872 1170 2599 1606 Fragments arranged by size: 2599 1606 1170 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6247 AvaI C'yCGr_G Cuts at: 1870 1943 3572 1870 Size: 73 1629 4545 Fragments arranged by size: 4545 1629 73 BamHI G'GATC_C Cuts at: 3577 3944 3577 Size: 367 5880 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2126 2213 3568 5023 618 Size: 325 217 627 339 87 1355 1455 1842 Fragments arranged by size: 1842 1455 1355 627 339 325 217 87 BanII G_rGCy'C Cuts at: 1391 3566 1391 Size: 2175 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5276 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3285 3319 4438 4472 1903 Size: 34 1348 34 1119 34 3678 Fragments arranged by size: 3678 1348 1119 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4139 5349 4139 Size: 1210 5037 BclI T'GATC_A Cuts at: 1548 1548 Size: 6247 BfrBI ATG'CAT Cuts at: 3306 3306 Size: 6247 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4858 136 Size: 107 122 71 631 605 3186 1525 Fragments arranged by size: 3186 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6242 6242 Size: 6247 BlpI GC'TnA_GC Cuts at: 3664 3664 Size: 6247 Bme1580I G_kGCm'C Cuts at: 1790 2131 4308 5464 1790 Size: 341 2177 1156 2573 Fragments arranged by size: 2573 2177 1156 341 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6247 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3978 4936 442 Size: 416 53 83 399 24 2561 958 1753 Fragments arranged by size: 2561 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2784 1056 Size: 1728 4519 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2589 2877 4907 1239 Size: 1350 288 2030 2579 Fragments arranged by size: 2579 2030 1350 288 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4372 5237 5535 4372 Size: 865 298 5084 Fragments arranged by size: 5084 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4910 882 Size: 617 3411 2219 Fragments arranged by size: 3411 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4038 493 Size: 711 2834 2702 Fragments arranged by size: 2834 2702 711 BsaBI GATnn'nnATC Cuts at: 2156 3305 6241 2156 Size: 1149 2936 2162 Fragments arranged by size: 2936 2162 1149 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4054 4436 5071 275 Size: 53 83 186 936 2521 382 635 1451 Fragments arranged by size: 2521 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4680 5078 5352 5499 5910 4680 Size: 398 274 147 411 5017 Fragments arranged by size: 5017 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3480 3510 1178 Size: 30 165 30 2077 30 3915 Fragments arranged by size: 3915 2077 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2420 1186 Size: 1234 5013 BseYI C'CCAG_C Cuts at: 5450 5946 5450 Size: 496 5751 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5674 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3697 4458 5486 1540 Size: 275 186 1696 761 1028 2301 Fragments arranged by size: 2301 1696 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2419 3430 4308 4393 5464 2419 Size: 1011 878 85 1071 3202 Fragments arranged by size: 3202 1071 1011 878 85 BsmI GAATG_Cn' Cuts at: 2994 3484 3758 3851 2994 Size: 490 274 93 5390 Fragments arranged by size: 5390 490 274 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2131 2419 3430 3566 4308 4393 5464 Size: 399 341 288 1011 136 742 85 1071 Cuts at: 5464 1391 Size: 2174 Fragments arranged by size: 2174 1071 1011 742 399 341 288 136 85 BspEI T'CCGG_A Cuts at: 5910 5910 Size: 6247 BspHI T'CATG_A Cuts at: 4136 5866 4136 Size: 1730 4517 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3357 3602 844 Size: 2513 245 3489 Fragments arranged by size: 3489 2513 245 BsrBI CCG'CTC Cuts at: 1394 1823 4134 1394 Size: 429 2311 3507 Fragments arranged by size: 3507 2311 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4742 4916 65 Size: 1183 3494 174 1396 Fragments arranged by size: 3494 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4891 5078 1700 Size: 110 3081 187 2869 Fragments arranged by size: 3081 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3339 3400 96 Size: 3243 61 2943 Fragments arranged by size: 3243 2943 61 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6247 BssSI C'ACGA_G Cuts at: 4307 5319 4307 Size: 1012 5235 BstAPI GCAn_nnn'nTGC Cuts at: 794 2333 2336 794 Size: 1539 3 4705 Fragments arranged by size: 4705 1539 3 BstBI TT'CG_AA Cuts at: 5051 5051 Size: 6247 BstEII G'GTnAC_C Cuts at: 1184 2771 1184 Size: 1587 4660 BstYI r'GATC_y Cuts at: 1972 2369 3577 3944 4329 4346 5798 5884 5896 Size: 397 1208 367 385 17 1452 86 12 Cuts at: 5896 6242 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1208 397 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2648 2648 Size: 6247 Bsu36I CC'TnA_GG Cuts at: 2788 2788 Size: 6247 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5695 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5508 Fragments arranged by size: 5508 683 56 BtsI GCAGTG_nn' Cuts at: 2023 3763 4557 4577 2023 Size: 1740 794 20 3693 Fragments arranged by size: 3693 1740 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6247 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6212 DraI TTT'AAA Cuts at: 3123 3551 3123 Size: 428 5819 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3152 5254 809 Size: 507 1836 2102 1802 Fragments arranged by size: 2102 1836 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3694 4583 6212 Size: 54 1425 50 275 18 1864 889 1629 Cuts at: 6212 8 Size: 43 Fragments arranged by size: 1864 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3694 1537 Size: 275 1882 4090 Fragments arranged by size: 4090 1882 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4177 1353 Size: 2824 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4834 5208 5354 387 Size: 1064 138 3245 374 146 1280 Fragments arranged by size: 3245 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2535 2589 2877 4310 4907 5694 5962 Size: 393 903 54 288 1433 597 787 268 Cuts at: 5962 1239 Size: 1524 Fragments arranged by size: 1524 1433 903 787 597 393 288 268 54 EcoO109I rG'GnC_Cy Cuts at: 2804 3076 2804 Size: 272 5975 EcoRI G'AATT_C Cuts at: 3556 3934 3556 Size: 378 5869 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2735 3402 2735 Size: 667 5580 FspI TGC'GCA Cuts at: 4753 4753 Size: 6247 HaeII r_GCGC'y Cuts at: 1678 5394 1678 Size: 3716 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2231 2263 2675 2707 3211 Size: 32 135 32 659 32 412 32 504 Cuts at: 3211 3243 3412 3444 4888 4920 4962 4994 1373 Size: 32 169 32 1444 32 42 32 2626 Fragments arranged by size: 2626 1444 659 504 412 169 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2029 2231 3591 3837 669 Size: 988 281 91 202 1360 246 3079 Fragments arranged by size: 3079 1360 988 281 246 202 91 HindIII A'AGCT_T Cuts at: 748 2295 2309 748 Size: 1547 14 4686 Fragments arranged by size: 4686 1547 14 HpaI GTT'AAC Cuts at: 3837 3837 Size: 6247 KpnI G_GTAC'C Cuts at: 2217 3572 2217 Size: 1355 4892 MfeI C'AATT_G Cuts at: 3148 3846 3148 Size: 698 5549 MluI A'CGCG_T Cuts at: 6123 6123 Size: 6247 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3230 3478 5361 5545 1305 Size: 1925 248 1883 184 2007 Fragments arranged by size: 2007 1925 1883 248 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4425 Fragments arranged by size: 4425 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2821 2895 3051 4205 4564 4723 518 Size: 648 1655 74 156 1154 359 159 2042 Fragments arranged by size: 2042 1655 1154 648 359 159 156 74 MspA1I CmG'CkG Cuts at: 1606 2144 2333 2342 2351 3663 4340 5488 5733 Size: 538 189 9 9 1312 677 1148 245 Cuts at: 5733 1606 Size: 2120 Fragments arranged by size: 2120 1312 1148 677 538 245 189 9 9 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5695 NdeI CA'TA_TG Cuts at: 387 387 Size: 6247 NheI G'CTAG_C Cuts at: 1052 2780 1052 Size: 1728 4519 NotI GC'GGCC_GC Cuts at: 3694 3694 Size: 6247 NsiI A_TGCA'T Cuts at: 3308 3308 Size: 6247 NspI r_CATG'y Cuts at: 1132 1229 3605 5150 1132 Size: 97 2376 1545 2229 Fragments arranged by size: 2376 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6247 PciI A'CATG_T Cuts at: 5146 5146 Size: 6247 PflMI CCAn_nnn'nTGG Cuts at: 1108 2757 1108 Size: 1649 4598 PmeI GTTT'AAAC Cuts at: 3551 3551 Size: 6247 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4316 4348 5829 5861 4316 Size: 32 1481 32 4702 Fragments arranged by size: 4702 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3076 3076 Size: 6247 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6247 PsiI TTA'TAA Cuts at: 3817 3817 Size: 6247 PstI C_TGCA'G Cuts at: 830 2341 2356 3599 830 Size: 1511 15 1243 3478 Fragments arranged by size: 3478 1511 1243 15 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6247 PvuII CAG'CTG Cuts at: 2333 2342 2351 2333 Size: 9 9 6229 Fragments arranged by size: 6229 9 9 SalI G'TCGA_C Cuts at: 1936 2229 3589 1936 Size: 293 1360 4594 Fragments arranged by size: 4594 1360 293 SanDI GG'GwC_CC Cuts at: 3076 3076 Size: 6247 SbfI CC_TGCA'GG Cuts at: 3599 3599 Size: 6247 ScaI AGT'ACT Cuts at: 2634 4495 6207 2634 Size: 1861 1712 2674 Fragments arranged by size: 2674 1861 1712 SfcI C'TryA_G Cuts at: 826 1045 2174 2337 2352 2424 3442 3595 4730 Size: 219 1129 163 15 72 1018 153 1135 Cuts at: 4730 5411 5602 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1129 1018 681 219 191 163 153 72 15 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6247 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6247 SmaI CCC'GGG Cuts at: 1872 3574 1872 Size: 1702 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4351 5252 5514 820 Size: 197 926 2408 901 262 1553 Fragments arranged by size: 2408 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4038 493 Size: 3545 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6247 SphI G_CATG'C Cuts at: 1132 3605 1132 Size: 2473 3774 SspI AAT'ATT Cuts at: 5 52 3462 4171 5 Size: 47 3410 709 2081 Fragments arranged by size: 3410 2081 709 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6247 StyI C'CwwG_G Cuts at: 513 1065 3040 3686 513 Size: 552 1975 646 3074 Fragments arranged by size: 3074 1975 646 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3309 4274 4291 4444 4629 5074 325 Size: 1255 1729 965 17 153 185 445 1498 Fragments arranged by size: 1729 1498 1255 965 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2278 2632 2981 Size: 274 80 33 51 1443 301 354 349 Cuts at: 2981 3339 3400 4493 6205 96 Size: 358 61 1093 1712 138 Fragments arranged by size: 1712 1443 1093 358 354 349 301 274 138 80 61 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2584 2616 6207 6239 2584 Size: 32 3591 32 2592 Fragments arranged by size: 3591 2592 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6247 XbaI T'CTAG_A Cuts at: 2695 3583 2695 Size: 888 5359 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6247 XmaI C'CCGG_G Cuts at: 1870 3572 1870 Size: 1702 4545 XmnI GAAnn'nnTTC Cuts at: 4376 4376 Size: 6247 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4055 276 Size: 53 83 186 936 2521 2468 Fragments arranged by size: 2521 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI