(Circular) (Six-base) MAPSORT of: pFN21AB5106.seq Check: 5152 from: 1 to: 7057 pFN21AB5106 7057 bp With 182 enzymes: SgfI * February 27, 2009 14:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3698 3698 Size: 7057 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4867 278 Size: 53 83 186 936 3331 2468 Fragments arranged by size: 3331 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2598 4400 1937 Size: 661 1802 4594 Fragments arranged by size: 4594 1802 661 Acc65I G'GTAC_C Cuts at: 2670 4378 2670 Size: 1708 5349 AclI AA'CG_TT Cuts at: 1563 4182 5184 5557 1563 Size: 2619 1002 373 3063 Fragments arranged by size: 3063 2619 1002 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2348 2841 4229 5120 6504 6772 1632 Size: 716 493 1388 891 1384 268 1917 Fragments arranged by size: 1917 1388 1384 891 716 493 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6860 AflIII A'CryG_T Cuts at: 1203 2903 2905 3445 4311 4336 5956 6933 1203 Size: 1700 2 540 866 25 1620 977 1327 Fragments arranged by size: 1700 1620 1327 977 866 540 25 2 AgeI A'CCGG_T Cuts at: 5888 5888 Size: 7057 AhdI GACnn_n'nnGTC Cuts at: 5786 5786 Size: 7057 AleI CACnn'nnGTG Cuts at: 1166 2906 1166 Size: 1740 5317 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2363 1184 Size: 1179 5878 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2699 2731 2699 Size: 32 7025 AlwNI CAG_nnn'CTG Cuts at: 4800 6372 4800 Size: 1572 5485 ApaI G_GGCC'C Cuts at: 2029 2029 Size: 7057 ApaLI G'TGCA_C Cuts at: 5114 6270 5114 Size: 1156 5901 ApoI r'AATT_y Cuts at: 1493 4133 4366 4592 4744 6853 1493 Size: 2640 233 226 152 2109 1697 Fragments arranged by size: 2640 2109 1697 233 226 152 AseI AT'TA_AT Cuts at: 160 1032 5611 160 Size: 872 4579 1606 Fragments arranged by size: 4579 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7057 AvaI C'yCGr_G Cuts at: 1870 1943 2022 2918 3454 3567 4116 4382 1870 Size: 73 79 896 536 113 549 266 4545 Fragments arranged by size: 4545 896 549 536 266 113 79 73 BamHI G'GATC_C Cuts at: 4387 4754 4387 Size: 367 6690 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2295 2543 2670 3262 4033 Size: 325 217 627 508 248 127 592 771 Cuts at: 4033 4378 5833 618 Size: 345 1455 1842 Fragments arranged by size: 1842 1455 771 627 592 508 345 325 248 217 127 BanII G_rGCy'C Cuts at: 1391 2029 2039 2522 3792 3831 4376 1391 Size: 638 10 483 1270 39 545 4072 Fragments arranged by size: 4072 1270 638 545 483 39 10 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3890 928 Size: 971 1991 4095 Fragments arranged by size: 4095 1991 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2065 2099 5248 5282 1903 Size: 34 128 34 3149 34 3678 Fragments arranged by size: 3678 3149 128 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4949 6159 4949 Size: 1210 5847 BclI T'GATC_A Cuts at: 1548 3214 3516 1548 Size: 1666 302 5089 Fragments arranged by size: 5089 1666 302 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2078 3423 5668 Size: 107 122 71 631 605 406 1345 2245 Cuts at: 5668 136 Size: 1525 Fragments arranged by size: 2245 1525 1345 631 605 406 122 107 71 BglII A'GATC_T Cuts at: 7052 7052 Size: 7057 BlpI GC'TnA_GC Cuts at: 3295 4474 3295 Size: 1179 5878 Bme1580I G_kGCm'C Cuts at: 1790 2029 2788 2925 3265 4036 5118 6274 1790 Size: 239 759 137 340 771 1082 1156 2573 Fragments arranged by size: 2573 1156 1082 771 759 340 239 137 BmgBI CAC'GTC Cuts at: 1318 4312 1318 Size: 2994 4063 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2248 2719 4788 Size: 416 53 83 399 24 831 471 2069 Cuts at: 4788 5746 442 Size: 958 1753 Fragments arranged by size: 2069 1753 958 831 471 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7057 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2080 2266 2080 Size: 186 6871 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2075 2236 2459 2666 3572 4369 5717 1239 Size: 836 161 223 207 906 797 1348 2579 Fragments arranged by size: 2579 1348 906 836 797 223 207 161 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2097 4091 4244 5182 6047 6345 2097 Size: 1994 153 938 865 298 2809 Fragments arranged by size: 2809 1994 938 865 298 153 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3740 5720 882 Size: 617 2241 1980 2219 Fragments arranged by size: 2241 2219 1980 617 BsaAI yAC'GTr Cuts at: 493 1204 2161 2906 2926 4848 493 Size: 711 957 745 20 1922 2702 Fragments arranged by size: 2702 1922 957 745 711 20 BsaBI GATnn'nnATC Cuts at: 7051 7051 Size: 7057 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2601 4864 5246 5881 Size: 53 83 186 936 1068 2263 382 635 Cuts at: 5881 275 Size: 1451 Fragments arranged by size: 2263 1451 1068 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2415 5490 5888 6162 6309 6720 2415 Size: 3075 398 274 147 411 2752 Fragments arranged by size: 3075 2752 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2744 2774 3435 3465 1178 Size: 30 165 30 1341 30 661 30 4770 Fragments arranged by size: 4770 1341 661 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2456 2765 2949 3139 3172 3596 1186 Size: 1270 309 184 190 33 424 4647 Fragments arranged by size: 4647 1270 424 309 190 184 33 BseYI C'CCAG_C Cuts at: 2745 2890 3025 3272 4009 4162 6260 6756 2745 Size: 145 135 247 737 153 2098 496 3046 Fragments arranged by size: 3046 2098 737 496 247 153 145 135 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2501 2561 2948 3107 3125 4361 1163 Size: 573 765 60 387 159 18 1236 3859 Fragments arranged by size: 3859 1236 765 573 387 159 60 18 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3288 3697 3798 4507 5268 6296 Size: 275 186 1287 409 101 709 761 1028 Cuts at: 6296 1540 Size: 2301 Fragments arranged by size: 2301 1287 1028 761 709 409 275 186 101 BsiHKAI G_wGCw'C Cuts at: 2061 3867 3912 4233 5118 5203 6274 2061 Size: 1806 45 321 885 85 1071 2844 Fragments arranged by size: 2844 1806 1071 885 321 85 45 BsiWI C'GTAC_G Cuts at: 3289 3289 Size: 7057 BsmI GAATG_Cn' Cuts at: 2951 4568 4661 2951 Size: 1617 93 5347 Fragments arranged by size: 5347 1617 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2029 2039 2061 2522 2788 2925 3265 Size: 399 239 10 22 461 266 137 340 Cuts at: 3265 3792 3831 3867 3912 4036 4233 4376 5118 Size: 527 39 36 45 124 197 143 742 Cuts at: 5118 5203 6274 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 527 461 399 340 266 239 197 143 137 124 85 45 39 36 22 10 BspEI T'CCGG_A Cuts at: 2415 6720 2415 Size: 4305 2752 BspHI T'CATG_A Cuts at: 2607 4275 4946 6676 2607 Size: 1668 671 1730 2988 Fragments arranged by size: 2988 1730 1668 671 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2352 3698 4412 844 Size: 1508 1346 714 3489 Fragments arranged by size: 3489 1508 1346 714 BsrBI CCG'CTC Cuts at: 1394 1823 4944 1394 Size: 429 3121 3507 Fragments arranged by size: 3507 3121 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3312 5552 5726 65 Size: 1183 2064 2240 174 1396 Fragments arranged by size: 2240 2064 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2072 3413 5701 5888 1700 Size: 110 262 1341 2288 187 2869 Fragments arranged by size: 2869 2288 1341 262 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7057 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7057 BssSI C'ACGA_G Cuts at: 3578 5117 6129 3578 Size: 1539 1012 4506 Fragments arranged by size: 4506 1539 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7057 BstBI TT'CG_AA Cuts at: 3822 5861 3822 Size: 2039 5018 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7057 BstXI CCAn_nnnn'nTGG Cuts at: 2977 3604 4084 2977 Size: 627 480 5950 Fragments arranged by size: 5950 627 480 BstYI r'GATC_y Cuts at: 1972 2961 3219 3844 4387 4754 5139 5156 6608 Size: 989 258 625 543 367 385 17 1452 Cuts at: 6608 6694 6706 7052 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 989 625 543 385 367 346 258 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2139 2613 3267 3597 3750 513 Size: 552 939 135 474 654 330 153 3820 Fragments arranged by size: 3820 939 654 552 474 330 153 135 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2209 2980 3128 487 Size: 683 56 983 771 148 4416 Fragments arranged by size: 4416 983 771 683 148 56 BtsI GCAGTG_nn' Cuts at: 2293 3934 4095 4573 5367 5387 2293 Size: 1641 161 478 794 20 3963 Fragments arranged by size: 3963 1641 794 478 161 20 ClaI AT'CG_AT Cuts at: 1554 2466 1554 Size: 912 6145 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7022 DraI TTT'AAA Cuts at: 4361 4361 Size: 7057 DraIII CAC_nnn'GTG Cuts at: 3061 3061 Size: 7057 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6064 809 Size: 507 4748 1802 Fragments arranged by size: 4748 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2448 2458 2967 Size: 54 1425 50 275 18 618 10 509 Cuts at: 2967 3285 3322 3415 3774 3795 4287 4504 5393 Size: 318 37 93 359 21 492 217 889 Cuts at: 5393 7022 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 618 509 492 359 318 275 217 93 54 50 43 37 21 18 10 EagI C'GGCC_G Cuts at: 1537 1812 3285 3795 4504 1537 Size: 275 1473 510 709 4090 Fragments arranged by size: 4090 1473 709 510 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2417 3465 3819 3966 4987 1353 Size: 1064 1048 354 147 1021 3423 Fragments arranged by size: 3423 1064 1048 1021 354 147 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2560 3700 5644 6018 6164 387 Size: 1064 138 971 1140 1944 374 146 1280 Fragments arranged by size: 1944 1280 1140 1064 971 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2075 2236 2348 2459 2666 2841 3572 Size: 393 443 161 112 111 207 175 731 Cuts at: 3572 4229 4369 5120 5717 6504 6772 1239 Size: 657 140 751 597 787 268 1524 Fragments arranged by size: 1524 787 751 731 657 597 443 393 268 207 175 161 140 112 111 EcoO109I rG'GnC_Cy Cuts at: 2026 2634 2940 2026 Size: 608 306 6143 Fragments arranged by size: 6143 608 306 EcoRI G'AATT_C Cuts at: 4366 4744 4366 Size: 378 6679 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2056 2194 2056 Size: 138 6919 FspI TGC'GCA Cuts at: 2147 3491 5563 2147 Size: 1344 2072 3641 Fragments arranged by size: 3641 2072 1344 FspAI rTGC'GCAy Cuts at: 2147 3491 2147 Size: 1344 5713 HaeII r_GCGC'y Cuts at: 1678 6204 1678 Size: 4526 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2201 2233 3183 3215 3836 Size: 32 135 32 629 32 950 32 621 Cuts at: 3836 3868 5698 5730 5772 5804 1373 Size: 32 1830 32 42 32 2626 Fragments arranged by size: 2626 1830 950 629 621 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2599 4401 4647 669 Size: 988 281 661 1802 246 3079 Fragments arranged by size: 3079 1802 988 661 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7057 HpaI GTT'AAC Cuts at: 4647 4647 Size: 7057 KpnI G_GTAC'C Cuts at: 2674 4382 2674 Size: 1708 5349 MfeI C'AATT_G Cuts at: 2829 3232 4656 2829 Size: 403 1424 5230 Fragments arranged by size: 5230 1424 403 MluI A'CGCG_T Cuts at: 3445 6933 3445 Size: 3488 3569 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3648 3879 4296 6171 6355 1305 Size: 2343 231 417 1875 184 2007 Fragments arranged by size: 2343 2007 1875 417 231 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2450 2969 3776 4289 10 Size: 54 1425 343 618 519 807 513 2778 Fragments arranged by size: 2778 1425 807 618 519 513 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2087 2549 2906 2981 3254 3485 3602 Size: 648 921 462 357 75 273 231 117 Cuts at: 3602 4157 5015 5374 5533 518 Size: 555 858 359 159 2042 Fragments arranged by size: 2042 921 858 648 555 462 359 357 273 231 159 117 75 MspA1I CmG'CkG Cuts at: 1606 2100 2890 3073 3269 3299 3717 3961 4330 Size: 494 790 183 196 30 418 244 369 Cuts at: 4330 4473 5150 6298 6543 1606 Size: 143 677 1148 245 2120 Fragments arranged by size: 2120 1148 790 677 494 418 369 245 244 196 183 143 30 NaeI GCC'GGC Cuts at: 2074 2074 Size: 7057 NcoI C'CATG_G Cuts at: 513 1065 2004 3597 3750 513 Size: 552 939 1593 153 3820 Fragments arranged by size: 3820 1593 939 552 153 NdeI CA'TA_TG Cuts at: 387 387 Size: 7057 NgoMIV G'CCGG_C Cuts at: 2072 2072 Size: 7057 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7057 NotI GC'GGCC_GC Cuts at: 4504 4504 Size: 7057 NspI r_CATG'y Cuts at: 1132 1229 4021 4340 4415 5960 1132 Size: 97 2792 319 75 1545 2229 Fragments arranged by size: 2792 2229 1545 319 97 75 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7057 PciI A'CATG_T Cuts at: 4336 5956 4336 Size: 1620 5437 PflMI CCAn_nnn'nTGG Cuts at: 1108 2203 1108 Size: 1095 5962 PfoI T'CCnGG_A Cuts at: 2052 3549 2052 Size: 1497 5560 PmeI GTTT'AAAC Cuts at: 4361 4361 Size: 7057 PmlI CAC'GTG Cuts at: 2906 2926 2906 Size: 20 7037 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5126 5158 6639 6671 5126 Size: 32 1481 32 5512 Fragments arranged by size: 5512 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2634 2940 2634 Size: 306 6751 PshAI GACnn'nnGTC Cuts at: 1613 3398 1613 Size: 1785 5272 PsiI TTA'TAA Cuts at: 4627 4627 Size: 7057 PspOMI G'GGCC_C Cuts at: 2025 2025 Size: 7057 PstI C_TGCA'G Cuts at: 830 2352 3852 3929 3998 4409 830 Size: 1522 1500 77 69 411 3478 Fragments arranged by size: 3478 1522 1500 411 77 69 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7057 PvuII CAG'CTG Cuts at: 2890 3299 3961 4330 2890 Size: 409 662 369 5617 Fragments arranged by size: 5617 662 409 369 SacII CC_GC'GG Cuts at: 3270 3270 Size: 7057 SalI G'TCGA_C Cuts at: 1936 2597 4399 1936 Size: 661 1802 4594 Fragments arranged by size: 4594 1802 661 SanDI GG'GwC_CC Cuts at: 2940 2940 Size: 7057 SapI GCTCTTCn'nnn_ Cuts at: 3819 3819 Size: 7057 SbfI CC_TGCA'GG Cuts at: 4409 4409 Size: 7057 ScaI AGT'ACT Cuts at: 3464 5305 7017 3464 Size: 1841 1712 3504 Fragments arranged by size: 3504 1841 1712 SfcI C'TryA_G Cuts at: 826 1045 2348 3848 3925 3994 4405 5540 6221 Size: 219 1303 1500 77 69 411 1135 681 Cuts at: 6221 6412 826 Size: 191 1471 Fragments arranged by size: 1500 1471 1303 1135 681 411 219 191 77 69 SfiI GGCCn_nnn'nGGCC Cuts at: 2078 2078 Size: 7057 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7057 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7057 SmaI CCC'GGG Cuts at: 1872 2024 3456 3569 4118 4384 1872 Size: 152 1432 113 549 266 4545 Fragments arranged by size: 4545 1432 549 266 152 113 SmlI C'TyrA_G Cuts at: 820 1017 1943 2112 4106 4259 5161 6062 6324 Size: 197 926 169 1994 153 902 901 262 Cuts at: 6324 820 Size: 1553 Fragments arranged by size: 1994 1553 926 902 901 262 197 169 153 SnaBI TAC'GTA Cuts at: 493 4848 493 Size: 4355 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7057 SphI G_CATG'C Cuts at: 1132 4415 1132 Size: 3283 3774 SspI AAT'ATT Cuts at: 5 52 2806 4981 5 Size: 47 2754 2175 2081 Fragments arranged by size: 2754 2175 2081 47 StuI AGG'CCT Cuts at: 1517 2303 3536 3903 1517 Size: 786 1233 367 4671 Fragments arranged by size: 4671 1233 786 367 StyI C'CwwG_G Cuts at: 513 1065 2004 2031 2169 2691 3366 3418 3597 Size: 552 939 27 138 522 675 52 179 Cuts at: 3597 3750 4496 513 Size: 153 746 3074 Fragments arranged by size: 3074 939 746 675 552 522 179 153 138 52 27 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2701 5084 5101 5254 5439 5884 325 Size: 1255 1121 2383 17 153 185 445 1498 Fragments arranged by size: 2383 1498 1255 1121 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2175 3462 4293 Size: 274 80 33 51 1443 198 1287 831 Cuts at: 4293 5303 7015 96 Size: 1010 1712 138 Fragments arranged by size: 1712 1443 1287 1010 831 274 198 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2744 2776 7017 7049 2744 Size: 32 4241 32 2752 Fragments arranged by size: 4241 2752 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7057 XbaI T'CTAG_A Cuts at: 4393 4393 Size: 7057 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2742 4159 2742 Size: 1417 5640 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7057 XmaI C'CCGG_G Cuts at: 1870 2022 3454 3567 4116 4382 1870 Size: 152 1432 113 549 266 4545 Fragments arranged by size: 4545 1432 549 266 152 113 XmnI GAAnn'nnTTC Cuts at: 5186 5186 Size: 7057 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4865 276 Size: 53 83 186 936 3331 2468 Fragments arranged by size: 3331 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI KasI NarI NruI NsiI PacI PspXI PsrI RsrII SacI SexAI SfoI SrfI SwaI