Restriction map for pFN21AB5104
 (Circular) (Six-base) MAPSORT of: pFN21AB5104.seq  Check: 8883  from: 1  to: 6349



pFN21AB5104  6349 bp

 With 182 enzymes: SgfI * 

                        February 27, 2009 14:34  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    4159     278 
   Size:         53      83     186     936    2623    2468

  Fragments arranged by size: 

                2623    2468     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3692    1937 
   Size:       1755    4594
 
Acc65I G'GTAC_C

Cuts at:   3670    3670 
   Size:       6349
 
AclI AA'CG_TT

Cuts at:   1563    4476    4849    1563 
   Size:       2913     373    3063

  Fragments arranged by size: 

                3063    2913     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2040    2130    2183    2276    4412    5796    6064    1632 
   Size:        408      90      53      93    2136    1384     268    1917

  Fragments arranged by size: 

                2136    1917    1384     408     268      93      90      53
            
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    6152
 
AflIII A'CryG_T

Cuts at:   1203    2669    5248    6225    1203 
   Size:       1466    2579     977    1327

  Fragments arranged by size: 

                2579    1466    1327     977
 
AgeI A'CCGG_T

Cuts at:   5180    5180 
   Size:       6349
 
AhdI GACnn_n'nnGTC

Cuts at:   5078    5078 
   Size:       6349
 
AleI CACnn'nnGTG

Cuts at:   1166    2258    1166 
   Size:       1092    5257
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       6349
 
AlwNI CAG_nnn'CTG

Cuts at:   2299    2302    4092    5664    2299 
   Size:          3    1790    1572    2984

  Fragments arranged by size: 

                2984    1790    1572       3
 
ApaLI G'TGCA_C

Cuts at:   4406    5562    4406 
   Size:       1156    5193
 
ApoI r'AATT_y

Cuts at:   1493    2190    2309    2426    2660    3016    3562    3658    3884 
   Size:        697     119     117     234     356     546      96     226

Cuts at:   3884    4036    6145    1493 
   Size:        152    2109    1697

  Fragments arranged by size: 

                2109    1697     697     546     356     234     226     152
                 119     117      96
 
AseI AT'TA_AT

Cuts at:    160    1032    3207    4903     160 
   Size:        872    2175    1696    1606

  Fragments arranged by size: 

                2175    1696    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6349
 
AvaI C'yCGr_G

Cuts at:   1870    1943    3674    1870 
   Size:         73    1731    4545

  Fragments arranged by size: 

                4545    1731      73
 
BamHI G'GATC_C

Cuts at:   3679    4046    3679 
   Size:        367    5982
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2104    3361    3670    5125     618 
   Size:        325     217     627     317    1257     309    1455    1842

  Fragments arranged by size: 

                1842    1455    1257     627     325     317     309     217
            
 
BanII G_rGCy'C

Cuts at:   1391    2074    3000    3668    1391 
   Size:        683     926     668    4072

  Fragments arranged by size: 

                4072     926     683     668
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    3093     928 
   Size:        971    1194    4184

  Fragments arranged by size: 

                4184    1194     971
 
BbvCI CC'TCA_GC

Cuts at:   2421    2421 
   Size:       6349
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    4540    4574    1903 
   Size:         34    2603      34    3678

  Fragments arranged by size: 

                3678    2603      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   4241    5451    4241 
   Size:       1210    5139
 
BclI T'GATC_A

Cuts at:   1548    2412    1548 
   Size:        864    5485
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4960     136 
   Size:        107     122      71     631     605    3288    1525

  Fragments arranged by size: 

                3288    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   6344    6344 
   Size:       6349
 
BlpI GC'TnA_GC

Cuts at:   3766    3766 
   Size:       6349
 
Bme1580I G_kGCm'C

Cuts at:   1790    2107    3322    4410    5566    1790 
   Size:        317    1215    1088    1156    2573

  Fragments arranged by size: 

                2573    1215    1156    1088     317
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       6349
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    4080    5038     442 
   Size:        416      53      83     399      24    2663     958    1753

  Fragments arranged by size: 

                2663    1753     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       6349
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    3333    3575    5009    1239 
   Size:       2094     242    1434    2579

  Fragments arranged by size: 

                2579    2094    1434     242
 
Bpu10I CC'TnA_GC

Cuts at:   2421    2421 
   Size:       6349
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3116    3256    4474    5339    5637    3116 
   Size:        140    1218     865     298    3828

  Fragments arranged by size: 

                3828    1218     865     298     140
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    5012     882 
   Size:        617    3513    2219

  Fragments arranged by size: 

                3513    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    4140     493 
   Size:        711    2936    2702

  Fragments arranged by size: 

                2936    2702     711
 
BsaBI GATnn'nnATC

Cuts at:   6343    6343 
   Size:       6349
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3631    4156    4538    5173 
   Size:         53      83     186     936    2098     525     382     635

Cuts at:   5173     275 
   Size:       1451

  Fragments arranged by size: 

                2098    1451     936     635     525     382     186      83
                  53
 
BsaWI w'CCGG_w

Cuts at:   3020    4782    5180    5454    5601    6012    3020 
   Size:       1762     398     274     147     411    3357

  Fragments arranged by size: 

                3357    1762     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    6124

  Fragments arranged by size: 

                6124     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       6349
 
BseYI C'CCAG_C

Cuts at:   2999    5552    6048    2999 
   Size:       2553     496    3300

  Fragments arranged by size: 

                3300    2553     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    5776
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3799    4560    5588    1540 
   Size:        275     186    1798     761    1028    2301

  Fragments arranged by size: 

                2301    1798    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2074    4410    4495    5566    2074 
   Size:       2336      85    1071    2857

  Fragments arranged by size: 

                2857    2336    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2358    2458    3860    3953    2358 
   Size:        100    1402      93    4754

  Fragments arranged by size: 

                4754    1402     100      93
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2074    2107    3000    3322    3668    4410    4495 
   Size:        399     284      33     893     322     346     742      85

Cuts at:   4495    5566    1391 
   Size:       1071    2174

  Fragments arranged by size: 

                2174    1071     893     742     399     346     322     284
                  85      33
 
BspEI T'CCGG_A

Cuts at:   3020    6012    3020 
   Size:       2992    3357
 
BspHI T'CATG_A

Cuts at:   4238    5968    4238 
   Size:       1730    4619
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    3704     844 
   Size:       2860    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    4236    1394 
   Size:        429    2413    3507

  Fragments arranged by size: 

                3507    2413     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4844    5018      65 
   Size:       1183    3596     174    1396

  Fragments arranged by size: 

                3596    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4993    5180    1700 
   Size:        110    3183     187    2869

  Fragments arranged by size: 

                3183    2869     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       6349
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       6349
 
BssSI C'ACGA_G

Cuts at:   4409    5421    4409 
   Size:       1012    5337
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    2302    2741     794 
   Size:       1508     439    4402

  Fragments arranged by size: 

                4402    1508     439
 
BstBI TT'CG_AA

Cuts at:   5153    5153 
   Size:       6349
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       6349
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2540    2947    3007    2540 
   Size:        407      60    5882

  Fragments arranged by size: 

                5882     407      60
 
BstYI r'GATC_y

Cuts at:   1972    2365    2725    3679    4046    4431    4448    5900    5986 
   Size:        393     360     954     367     385      17    1452      86

Cuts at:   5986    5998    6344    1972 
   Size:         12     346    1977

  Fragments arranged by size: 

                1977    1452     954     393     385     367     360     346
                  86      17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    2940     513 
   Size:        552     939     936    3922

  Fragments arranged by size: 

                3922     939     936     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    5610

  Fragments arranged by size: 

                5610     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2265    3865    4659    4679    2265 
   Size:       1600     794      20    3935

  Fragments arranged by size: 

                3935    1600     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       6349
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    6314
 
DraI TTT'AAA

Cuts at:   2445    3653    2445 
   Size:       1208    5141
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    5356     809 
   Size:        507    4040    1802

  Fragments arranged by size: 

                4040    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    3796    4685    6314 
   Size:         54    1425      50     275      18    1966     889    1629

Cuts at:   6314       8 
   Size:         43

  Fragments arranged by size: 

                1966    1629    1425     889     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3796    1537 
   Size:        275    1984    4090

  Fragments arranged by size: 

                4090    1984     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2334    3576    4279    1353 
   Size:        981    1242     703    3423

  Fragments arranged by size: 

                3423    1242     981     703
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4936    5310    5456     387 
   Size:       1064     138    3347     374     146    1280

  Fragments arranged by size: 

                3347    1280    1064     374     146     138
 
EcoICRI GAG'CTC

Cuts at:   2072    2072 
   Size:       6349
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2040    2130    2183    2276    3333    3575    4412 
   Size:        393     408      90      53      93    1057     242     837

Cuts at:   4412    5009    5796    6064    1239 
   Size:        597     787     268    1524

  Fragments arranged by size: 

                1524    1057     837     787     597     408     393     268
                 242      93      90      53
 
EcoO109I rG'GnC_Cy

Cuts at:   2598    2598 
   Size:       6349
 
EcoRI G'AATT_C

Cuts at:   2426    2660    3658    4036    2426 
   Size:        234     998     378    4739

  Fragments arranged by size: 

                4739     998     378     234
 
EcoRV GAT'ATC

Cuts at:   2578    2578 
   Size:       6349
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2115    2306    2746    2115 
   Size:        191     440    5718

  Fragments arranged by size: 

                5718     440     191
 
FspI TGC'GCA

Cuts at:   4855    4855 
   Size:       6349
 
HaeII r_GCGC'y

Cuts at:   1678    5496    1678 
   Size:       3818    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2115    2147    3362    3394    4990 
   Size:         32     135      32     543      32    1215      32    1596

Cuts at:   4990    5022    5064    5096    1373 
   Size:         32      42      32    2626

  Fragments arranged by size: 

                2626    1596    1215     543     135      42      32      32
                  32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3258    3693    3939     669 
   Size:        988     281    1320     435     246    3079

  Fragments arranged by size: 

                3079    1320     988     435     281     246
 
HindIII A'AGCT_T

Cuts at:    748    3240     748 
   Size:       2492    3857
 
HpaI GTT'AAC

Cuts at:   3258    3939    3258 
   Size:        681    5668
 
KpnI G_GTAC'C

Cuts at:   3674    3674 
   Size:       6349
 
MfeI C'AATT_G

Cuts at:   3948    3948 
   Size:       6349
 
MluI A'CGCG_T

Cuts at:   6225    6225 
   Size:       6349
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    3185    3388    5463    5647    1305 
   Size:       1880     203    2075     184    2007

  Fragments arranged by size: 

                2075    2007    1880     203     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    4527

  Fragments arranged by size: 

                4527    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2142    2258    2934    3396    4307    4666    4825 
   Size:        648     976     116     676     462     911     359     159

Cuts at:   4825     518 
   Size:       2042

  Fragments arranged by size: 

                2042     976     911     676     648     462     359     159
                 116
 
MspA1I CmG'CkG

Cuts at:   1606    2203    2299    2954    3765    4442    5590    5835    1606 
   Size:        597      96     655     811     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     811     677     655     597     245      96
            
 
NcoI C'CATG_G

Cuts at:    513    1065    2004    2940     513 
   Size:        552     939     936    3922

  Fragments arranged by size: 

                3922     939     936     552
 
NdeI CA'TA_TG

Cuts at:    387    2139     387 
   Size:       1752    4597
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       6349
 
NotI GC'GGCC_GC

Cuts at:   3796    3796 
   Size:       6349
 
NspI r_CATG'y

Cuts at:   1132    1229    2216    3707    5252    1132 
   Size:         97     987    1491    1545    2229

  Fragments arranged by size: 

                2229    1545    1491     987      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       6349
 
PciI A'CATG_T

Cuts at:   5248    5248 
   Size:       6349
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       6349
 
PmeI GTTT'AAAC

Cuts at:   3653    3653 
   Size:       6349
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4418    4450    5931    5963    4418 
   Size:         32    1481      32    4804

  Fragments arranged by size: 

                4804    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2598    2598 
   Size:       6349
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       6349
 
PsiI TTA'TAA

Cuts at:   2388    3919    2388 
   Size:       1531    4818
 
PstI C_TGCA'G

Cuts at:    830    3701     830 
   Size:       2871    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       6349
 
PvuII CAG'CTG

Cuts at:   2203    2299    2954    2203 
   Size:         96     655    5598

  Fragments arranged by size: 

                5598     655      96
 
SacI G_AGCT'C

Cuts at:   2074    2074 
   Size:       6349
 
SalI G'TCGA_C

Cuts at:   1936    3691    1936 
   Size:       1755    4594
 
SbfI CC_TGCA'GG

Cuts at:   3701    3701 
   Size:       6349
 
ScaI AGT'ACT

Cuts at:   4597    6309    4597 
   Size:       1712    4637
 
SfcI C'TryA_G

Cuts at:    826    1045    3179    3697    4832    5513    5704     826 
   Size:        219    2134     518    1135     681     191    1471

  Fragments arranged by size: 

                2134    1471    1135     681     518     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6349
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       6349
 
SmaI CCC'GGG

Cuts at:   1872    3676    1872 
   Size:       1804    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3131    3271    4453    5354    5616     820 
   Size:        197     926    1188     140    1182     901     262    1553

  Fragments arranged by size: 

                1553    1188    1182     926     901     262     197     140
            
 
SnaBI TAC'GTA

Cuts at:    493    4140     493 
   Size:       3647    2702
 
SpeI A'CTAG_T

Cuts at:    152    2990     152 
   Size:       2838    3511
 
SphI G_CATG'C

Cuts at:   1132    3707    1132 
   Size:       2575    3774
 
SspI AAT'ATT

Cuts at:      5      52    4273       5 
   Size:         47    4221    2081

  Fragments arranged by size: 

                4221    2081      47
 
StuI AGG'CCT

Cuts at:   1517    2763    1517 
   Size:       1246    5103
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2940    3788     513 
   Size:        552     939     936     848    3074

  Fragments arranged by size: 

                3074     939     936     848     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3265    4376    4393    4546    4731    5176     325 
   Size:       1255    1685    1111      17     153     185     445    1498

  Fragments arranged by size: 

                1685    1498    1255    1111     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    4595    6307      96 
   Size:        274      80      33      51    1443    2618    1712     138

  Fragments arranged by size: 

                2618    1712    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2661    2693    6309    6341    2661 
   Size:         32    3616      32    2669

  Fragments arranged by size: 

                3616    2669      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       6349
 
XbaI T'CTAG_A

Cuts at:   3685    3685 
   Size:       6349
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   3355    3355 
   Size:       6349
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       6349
 
XmaI C'CCGG_G

Cuts at:   1870    3674    1870 
   Size:       1804    4545
 
XmnI GAAnn'nnTTC

Cuts at:   2634    2660    3537    4478    2634 
   Size:         26     877     941    4505

  Fragments arranged by size: 

                4505     941     877      26
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    4157     276 
   Size:         53      83     186     936    2623    2468

  Fragments arranged by size: 

                2623    2468     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI    BbvCI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI    EcoRV     FalI     FspI
    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NcoI     NdeI     NheI     NotI
     NspI     PasI     PciI    PflMI     PmeI     PpiI    PpuMI    PshAI
     PsiI     PstI     PvuI    PvuII     SacI     SalI     SbfI     ScaI
     SfcI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BfrBI     BplI    BsiWI    BsmBI  BstZ17I   Bsu36I   DraIII    EcoNI
     FseI    FspAI     KasI     NaeI     NarI   NgoMIV     NruI     NsiI
     PacI     PfoI     PmlI   PspOMI    PspXI     PsrI    RsrII    SacII
    SanDI     SapI    SexAI     SfiI     SfoI     SrfI     SwaI
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