(Circular) (Six-base) MAPSORT of: pFN21AB5104.seq Check: 8883 from: 1 to: 6349 pFN21AB5104 6349 bp With 182 enzymes: SgfI * February 27, 2009 14:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4159 278 Size: 53 83 186 936 2623 2468 Fragments arranged by size: 2623 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3692 1937 Size: 1755 4594 Acc65I G'GTAC_C Cuts at: 3670 3670 Size: 6349 AclI AA'CG_TT Cuts at: 1563 4476 4849 1563 Size: 2913 373 3063 Fragments arranged by size: 3063 2913 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2040 2130 2183 2276 4412 5796 6064 1632 Size: 408 90 53 93 2136 1384 268 1917 Fragments arranged by size: 2136 1917 1384 408 268 93 90 53 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6152 AflIII A'CryG_T Cuts at: 1203 2669 5248 6225 1203 Size: 1466 2579 977 1327 Fragments arranged by size: 2579 1466 1327 977 AgeI A'CCGG_T Cuts at: 5180 5180 Size: 6349 AhdI GACnn_n'nnGTC Cuts at: 5078 5078 Size: 6349 AleI CACnn'nnGTG Cuts at: 1166 2258 1166 Size: 1092 5257 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6349 AlwNI CAG_nnn'CTG Cuts at: 2299 2302 4092 5664 2299 Size: 3 1790 1572 2984 Fragments arranged by size: 2984 1790 1572 3 ApaLI G'TGCA_C Cuts at: 4406 5562 4406 Size: 1156 5193 ApoI r'AATT_y Cuts at: 1493 2190 2309 2426 2660 3016 3562 3658 3884 Size: 697 119 117 234 356 546 96 226 Cuts at: 3884 4036 6145 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 697 546 356 234 226 152 119 117 96 AseI AT'TA_AT Cuts at: 160 1032 3207 4903 160 Size: 872 2175 1696 1606 Fragments arranged by size: 2175 1696 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6349 AvaI C'yCGr_G Cuts at: 1870 1943 3674 1870 Size: 73 1731 4545 Fragments arranged by size: 4545 1731 73 BamHI G'GATC_C Cuts at: 3679 4046 3679 Size: 367 5982 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2104 3361 3670 5125 618 Size: 325 217 627 317 1257 309 1455 1842 Fragments arranged by size: 1842 1455 1257 627 325 317 309 217 BanII G_rGCy'C Cuts at: 1391 2074 3000 3668 1391 Size: 683 926 668 4072 Fragments arranged by size: 4072 926 683 668 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3093 928 Size: 971 1194 4184 Fragments arranged by size: 4184 1194 971 BbvCI CC'TCA_GC Cuts at: 2421 2421 Size: 6349 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4540 4574 1903 Size: 34 2603 34 3678 Fragments arranged by size: 3678 2603 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4241 5451 4241 Size: 1210 5139 BclI T'GATC_A Cuts at: 1548 2412 1548 Size: 864 5485 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4960 136 Size: 107 122 71 631 605 3288 1525 Fragments arranged by size: 3288 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6344 6344 Size: 6349 BlpI GC'TnA_GC Cuts at: 3766 3766 Size: 6349 Bme1580I G_kGCm'C Cuts at: 1790 2107 3322 4410 5566 1790 Size: 317 1215 1088 1156 2573 Fragments arranged by size: 2573 1215 1156 1088 317 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6349 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4080 5038 442 Size: 416 53 83 399 24 2663 958 1753 Fragments arranged by size: 2663 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6349 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3333 3575 5009 1239 Size: 2094 242 1434 2579 Fragments arranged by size: 2579 2094 1434 242 Bpu10I CC'TnA_GC Cuts at: 2421 2421 Size: 6349 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3116 3256 4474 5339 5637 3116 Size: 140 1218 865 298 3828 Fragments arranged by size: 3828 1218 865 298 140 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5012 882 Size: 617 3513 2219 Fragments arranged by size: 3513 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4140 493 Size: 711 2936 2702 Fragments arranged by size: 2936 2702 711 BsaBI GATnn'nnATC Cuts at: 6343 6343 Size: 6349 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3631 4156 4538 5173 Size: 53 83 186 936 2098 525 382 635 Cuts at: 5173 275 Size: 1451 Fragments arranged by size: 2098 1451 936 635 525 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3020 4782 5180 5454 5601 6012 3020 Size: 1762 398 274 147 411 3357 Fragments arranged by size: 3357 1762 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6124 Fragments arranged by size: 6124 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6349 BseYI C'CCAG_C Cuts at: 2999 5552 6048 2999 Size: 2553 496 3300 Fragments arranged by size: 3300 2553 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5776 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3799 4560 5588 1540 Size: 275 186 1798 761 1028 2301 Fragments arranged by size: 2301 1798 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2074 4410 4495 5566 2074 Size: 2336 85 1071 2857 Fragments arranged by size: 2857 2336 1071 85 BsmI GAATG_Cn' Cuts at: 2358 2458 3860 3953 2358 Size: 100 1402 93 4754 Fragments arranged by size: 4754 1402 100 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2074 2107 3000 3322 3668 4410 4495 Size: 399 284 33 893 322 346 742 85 Cuts at: 4495 5566 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 893 742 399 346 322 284 85 33 BspEI T'CCGG_A Cuts at: 3020 6012 3020 Size: 2992 3357 BspHI T'CATG_A Cuts at: 4238 5968 4238 Size: 1730 4619 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3704 844 Size: 2860 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4236 1394 Size: 429 2413 3507 Fragments arranged by size: 3507 2413 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4844 5018 65 Size: 1183 3596 174 1396 Fragments arranged by size: 3596 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4993 5180 1700 Size: 110 3183 187 2869 Fragments arranged by size: 3183 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6349 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6349 BssSI C'ACGA_G Cuts at: 4409 5421 4409 Size: 1012 5337 BstAPI GCAn_nnn'nTGC Cuts at: 794 2302 2741 794 Size: 1508 439 4402 Fragments arranged by size: 4402 1508 439 BstBI TT'CG_AA Cuts at: 5153 5153 Size: 6349 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6349 BstXI CCAn_nnnn'nTGG Cuts at: 2540 2947 3007 2540 Size: 407 60 5882 Fragments arranged by size: 5882 407 60 BstYI r'GATC_y Cuts at: 1972 2365 2725 3679 4046 4431 4448 5900 5986 Size: 393 360 954 367 385 17 1452 86 Cuts at: 5986 5998 6344 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 954 393 385 367 360 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2940 513 Size: 552 939 936 3922 Fragments arranged by size: 3922 939 936 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5610 Fragments arranged by size: 5610 683 56 BtsI GCAGTG_nn' Cuts at: 2265 3865 4659 4679 2265 Size: 1600 794 20 3935 Fragments arranged by size: 3935 1600 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6349 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6314 DraI TTT'AAA Cuts at: 2445 3653 2445 Size: 1208 5141 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5356 809 Size: 507 4040 1802 Fragments arranged by size: 4040 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3796 4685 6314 Size: 54 1425 50 275 18 1966 889 1629 Cuts at: 6314 8 Size: 43 Fragments arranged by size: 1966 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3796 1537 Size: 275 1984 4090 Fragments arranged by size: 4090 1984 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2334 3576 4279 1353 Size: 981 1242 703 3423 Fragments arranged by size: 3423 1242 981 703 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4936 5310 5456 387 Size: 1064 138 3347 374 146 1280 Fragments arranged by size: 3347 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2072 2072 Size: 6349 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2040 2130 2183 2276 3333 3575 4412 Size: 393 408 90 53 93 1057 242 837 Cuts at: 4412 5009 5796 6064 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1057 837 787 597 408 393 268 242 93 90 53 EcoO109I rG'GnC_Cy Cuts at: 2598 2598 Size: 6349 EcoRI G'AATT_C Cuts at: 2426 2660 3658 4036 2426 Size: 234 998 378 4739 Fragments arranged by size: 4739 998 378 234 EcoRV GAT'ATC Cuts at: 2578 2578 Size: 6349 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2115 2306 2746 2115 Size: 191 440 5718 Fragments arranged by size: 5718 440 191 FspI TGC'GCA Cuts at: 4855 4855 Size: 6349 HaeII r_GCGC'y Cuts at: 1678 5496 1678 Size: 3818 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2115 2147 3362 3394 4990 Size: 32 135 32 543 32 1215 32 1596 Cuts at: 4990 5022 5064 5096 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1596 1215 543 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3258 3693 3939 669 Size: 988 281 1320 435 246 3079 Fragments arranged by size: 3079 1320 988 435 281 246 HindIII A'AGCT_T Cuts at: 748 3240 748 Size: 2492 3857 HpaI GTT'AAC Cuts at: 3258 3939 3258 Size: 681 5668 KpnI G_GTAC'C Cuts at: 3674 3674 Size: 6349 MfeI C'AATT_G Cuts at: 3948 3948 Size: 6349 MluI A'CGCG_T Cuts at: 6225 6225 Size: 6349 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3185 3388 5463 5647 1305 Size: 1880 203 2075 184 2007 Fragments arranged by size: 2075 2007 1880 203 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4527 Fragments arranged by size: 4527 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2142 2258 2934 3396 4307 4666 4825 Size: 648 976 116 676 462 911 359 159 Cuts at: 4825 518 Size: 2042 Fragments arranged by size: 2042 976 911 676 648 462 359 159 116 MspA1I CmG'CkG Cuts at: 1606 2203 2299 2954 3765 4442 5590 5835 1606 Size: 597 96 655 811 677 1148 245 2120 Fragments arranged by size: 2120 1148 811 677 655 597 245 96 NcoI C'CATG_G Cuts at: 513 1065 2004 2940 513 Size: 552 939 936 3922 Fragments arranged by size: 3922 939 936 552 NdeI CA'TA_TG Cuts at: 387 2139 387 Size: 1752 4597 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6349 NotI GC'GGCC_GC Cuts at: 3796 3796 Size: 6349 NspI r_CATG'y Cuts at: 1132 1229 2216 3707 5252 1132 Size: 97 987 1491 1545 2229 Fragments arranged by size: 2229 1545 1491 987 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6349 PciI A'CATG_T Cuts at: 5248 5248 Size: 6349 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6349 PmeI GTTT'AAAC Cuts at: 3653 3653 Size: 6349 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4418 4450 5931 5963 4418 Size: 32 1481 32 4804 Fragments arranged by size: 4804 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2598 2598 Size: 6349 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6349 PsiI TTA'TAA Cuts at: 2388 3919 2388 Size: 1531 4818 PstI C_TGCA'G Cuts at: 830 3701 830 Size: 2871 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6349 PvuII CAG'CTG Cuts at: 2203 2299 2954 2203 Size: 96 655 5598 Fragments arranged by size: 5598 655 96 SacI G_AGCT'C Cuts at: 2074 2074 Size: 6349 SalI G'TCGA_C Cuts at: 1936 3691 1936 Size: 1755 4594 SbfI CC_TGCA'GG Cuts at: 3701 3701 Size: 6349 ScaI AGT'ACT Cuts at: 4597 6309 4597 Size: 1712 4637 SfcI C'TryA_G Cuts at: 826 1045 3179 3697 4832 5513 5704 826 Size: 219 2134 518 1135 681 191 1471 Fragments arranged by size: 2134 1471 1135 681 518 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6349 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6349 SmaI CCC'GGG Cuts at: 1872 3676 1872 Size: 1804 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3131 3271 4453 5354 5616 820 Size: 197 926 1188 140 1182 901 262 1553 Fragments arranged by size: 1553 1188 1182 926 901 262 197 140 SnaBI TAC'GTA Cuts at: 493 4140 493 Size: 3647 2702 SpeI A'CTAG_T Cuts at: 152 2990 152 Size: 2838 3511 SphI G_CATG'C Cuts at: 1132 3707 1132 Size: 2575 3774 SspI AAT'ATT Cuts at: 5 52 4273 5 Size: 47 4221 2081 Fragments arranged by size: 4221 2081 47 StuI AGG'CCT Cuts at: 1517 2763 1517 Size: 1246 5103 StyI C'CwwG_G Cuts at: 513 1065 2004 2940 3788 513 Size: 552 939 936 848 3074 Fragments arranged by size: 3074 939 936 848 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3265 4376 4393 4546 4731 5176 325 Size: 1255 1685 1111 17 153 185 445 1498 Fragments arranged by size: 1685 1498 1255 1111 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4595 6307 96 Size: 274 80 33 51 1443 2618 1712 138 Fragments arranged by size: 2618 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2661 2693 6309 6341 2661 Size: 32 3616 32 2669 Fragments arranged by size: 3616 2669 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6349 XbaI T'CTAG_A Cuts at: 3685 3685 Size: 6349 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3355 3355 Size: 6349 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6349 XmaI C'CCGG_G Cuts at: 1870 3674 1870 Size: 1804 4545 XmnI GAAnn'nnTTC Cuts at: 2634 2660 3537 4478 2634 Size: 26 877 941 4505 Fragments arranged by size: 4505 941 877 26 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4157 276 Size: 53 83 186 936 2623 2468 Fragments arranged by size: 2623 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI