(Circular) (Six-base) MAPSORT of: pFN21AB5096.seq Check: 3944 from: 1 to: 6451 pFN21AB5096 6451 bp With 182 enzymes: SgfI * February 27, 2009 14:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4261 278 Size: 53 83 186 936 2725 2468 Fragments arranged by size: 2725 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3794 1937 Size: 1857 4594 Acc65I G'GTAC_C Cuts at: 3772 3772 Size: 6451 AclI AA'CG_TT Cuts at: 1563 4578 4951 1563 Size: 3015 373 3063 Fragments arranged by size: 3063 3015 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2086 3497 3673 4514 5898 6166 1632 Size: 454 1411 176 841 1384 268 1917 Fragments arranged by size: 1917 1411 1384 841 454 268 176 AfeI AGC'GCT Cuts at: 2171 2336 3020 2171 Size: 165 684 5602 Fragments arranged by size: 5602 684 165 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6254 AflIII A'CryG_T Cuts at: 1203 5350 6327 1203 Size: 4147 977 1327 Fragments arranged by size: 4147 1327 977 AgeI A'CCGG_T Cuts at: 5282 5282 Size: 6451 AhdI GACnn_n'nnGTC Cuts at: 5180 5180 Size: 6451 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6451 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6451 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3008 3040 3008 Size: 32 6419 AlwNI CAG_nnn'CTG Cuts at: 3475 4194 5766 3475 Size: 719 1572 4160 Fragments arranged by size: 4160 1572 719 ApaI G_GGCC'C Cuts at: 2952 2952 Size: 6451 ApaLI G'TGCA_C Cuts at: 2717 4508 5664 2717 Size: 1791 1156 3504 Fragments arranged by size: 3504 1791 1156 ApoI r'AATT_y Cuts at: 1493 2806 3137 3458 3760 3986 4138 6247 1493 Size: 1313 331 321 302 226 152 2109 1697 Fragments arranged by size: 2109 1697 1313 331 321 302 226 152 AseI AT'TA_AT Cuts at: 160 1032 5005 160 Size: 872 3973 1606 Fragments arranged by size: 3973 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6451 AvaI C'yCGr_G Cuts at: 1870 1943 2144 2744 3776 1870 Size: 73 201 600 1032 4545 Fragments arranged by size: 4545 1032 600 201 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2140 2173 2140 Size: 33 6418 BamHI G'GATC_C Cuts at: 3378 3781 4148 3378 Size: 403 367 5681 Fragments arranged by size: 5681 403 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2060 2271 2488 2776 3772 Size: 325 217 627 273 211 217 288 996 Cuts at: 3772 5227 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 996 627 325 288 273 217 217 211 BanII G_rGCy'C Cuts at: 1391 2745 2952 3330 3770 1391 Size: 1354 207 378 440 4072 Fragments arranged by size: 4072 1354 440 378 207 BbeI G_GCGC'C Cuts at: 2275 2275 Size: 6451 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3001 3136 3415 3673 928 Size: 971 1102 135 279 258 3706 Fragments arranged by size: 3706 1102 971 279 258 135 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2050 2084 2560 2594 4642 4676 1903 Size: 34 113 34 476 34 2048 34 3678 Fragments arranged by size: 3678 2048 476 113 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3053 4343 5553 3053 Size: 1290 1210 3951 Fragments arranged by size: 3951 1290 1210 BclI T'GATC_A Cuts at: 1548 3576 1548 Size: 2028 4423 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2774 5062 136 Size: 107 122 71 631 605 1102 2288 1525 Fragments arranged by size: 2288 1525 1102 631 605 122 107 71 BglII A'GATC_T Cuts at: 6446 6446 Size: 6451 BlpI GC'TnA_GC Cuts at: 3868 3868 Size: 6451 Bme1580I G_kGCm'C Cuts at: 1790 2493 2721 2952 4512 5668 1790 Size: 703 228 231 1560 1156 2573 Fragments arranged by size: 2573 1560 1156 703 231 228 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6451 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2173 4182 5140 Size: 416 53 83 399 24 756 2009 958 Cuts at: 5140 442 Size: 1753 Fragments arranged by size: 2009 1753 958 756 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6451 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5111 1239 Size: 3872 2579 Bpu10I CC'TnA_GC Cuts at: 3329 3329 Size: 6451 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4576 5441 5739 4576 Size: 865 298 5288 Fragments arranged by size: 5288 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2357 5114 882 Size: 617 858 2757 2219 Fragments arranged by size: 2757 2219 858 617 BsaAI yAC'GTr Cuts at: 493 1204 2156 4242 493 Size: 711 952 2086 2702 Fragments arranged by size: 2702 2086 952 711 BsaBI GATnn'nnATC Cuts at: 6445 6445 Size: 6451 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2272 3182 3694 4258 Size: 53 83 186 936 739 910 512 564 Cuts at: 4258 4640 5275 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 910 739 635 564 512 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4884 5282 5556 5703 6114 4884 Size: 398 274 147 411 5221 Fragments arranged by size: 5221 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3008 3038 1178 Size: 30 165 30 1605 30 4591 Fragments arranged by size: 4591 1605 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2649 3575 3578 3611 1186 Size: 1463 926 3 33 4026 Fragments arranged by size: 4026 1463 926 33 3 BseYI C'CCAG_C Cuts at: 2725 3220 5654 6150 2725 Size: 495 2434 496 3026 Fragments arranged by size: 3026 2434 496 495 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5878 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3545 3901 4662 5690 1540 Size: 275 186 1544 356 761 1028 2301 Fragments arranged by size: 2301 1544 1028 761 356 275 186 BsiHKAI G_wGCw'C Cuts at: 2721 3330 4512 4597 5668 2721 Size: 609 1182 85 1071 3504 Fragments arranged by size: 3504 1182 1071 609 85 BsmI GAATG_Cn' Cuts at: 2749 3962 4055 2749 Size: 1213 93 5145 Fragments arranged by size: 5145 1213 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3560 3560 Size: 6451 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2493 2721 2745 2952 3330 3770 4512 Size: 399 703 228 24 207 378 440 742 Cuts at: 4512 4597 5668 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 703 440 399 378 228 207 85 24 BspEI T'CCGG_A Cuts at: 6114 6114 Size: 6451 BspHI T'CATG_A Cuts at: 2535 4340 6070 2535 Size: 1805 1730 2916 Fragments arranged by size: 2916 1805 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2145 3806 844 Size: 1301 1661 3489 Fragments arranged by size: 3489 1661 1301 BsrBI CCG'CTC Cuts at: 1394 1823 4338 1394 Size: 429 2515 3507 Fragments arranged by size: 3507 2515 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4946 5120 65 Size: 1183 3698 174 1396 Fragments arranged by size: 3698 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3540 5095 5282 1700 Size: 110 1730 1555 187 2869 Fragments arranged by size: 2869 1730 1555 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6451 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6451 BssSI C'ACGA_G Cuts at: 2720 4511 5523 2720 Size: 1791 1012 3648 Fragments arranged by size: 3648 1791 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6451 BstBI TT'CG_AA Cuts at: 2326 5255 2326 Size: 2929 3522 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6451 BstYI r'GATC_y Cuts at: 1972 2683 3378 3781 4148 4533 4550 6002 6088 Size: 711 695 403 367 385 17 1452 86 Cuts at: 6088 6100 6446 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 711 695 403 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3509 3509 Size: 6451 BtgI C'CryG_G Cuts at: 513 1065 2560 2979 3295 3627 3697 513 Size: 552 1495 419 316 332 70 3267 Fragments arranged by size: 3267 1495 552 419 332 316 70 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2182 3429 487 Size: 683 56 956 1247 3509 Fragments arranged by size: 3509 1247 956 683 56 BtsI GCAGTG_nn' Cuts at: 2471 3111 3342 3967 4761 4781 2471 Size: 640 231 625 794 20 4141 Fragments arranged by size: 4141 794 640 625 231 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6451 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6416 DraI TTT'AAA Cuts at: 3755 3755 Size: 6451 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5458 809 Size: 507 4142 1802 Fragments arranged by size: 4142 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2193 2766 2982 Size: 54 1425 50 275 18 363 573 216 Cuts at: 2982 3076 3542 3898 4787 6416 8 Size: 94 466 356 889 1629 43 Fragments arranged by size: 1629 1425 889 573 466 363 356 275 216 94 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3542 3898 1537 Size: 275 1730 356 4090 Fragments arranged by size: 4090 1730 356 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3266 3318 4381 1353 Size: 1913 52 1063 3423 Fragments arranged by size: 3423 1913 1063 52 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5038 5412 5558 387 Size: 1064 138 3449 374 146 1280 Fragments arranged by size: 3449 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3328 3328 Size: 6451 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2086 3497 3673 4514 5111 5898 6166 Size: 393 454 1411 176 841 597 787 268 Cuts at: 6166 1239 Size: 1524 Fragments arranged by size: 1524 1411 841 787 597 454 393 268 176 EcoNI CCTnn'n_nnAGG Cuts at: 3121 3121 Size: 6451 EcoO109I rG'GnC_Cy Cuts at: 2149 2621 3091 3702 2149 Size: 472 470 611 4898 Fragments arranged by size: 4898 611 472 470 EcoRI G'AATT_C Cuts at: 3760 4138 3760 Size: 378 6073 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3304 3304 Size: 6451 FseI GG_CCGG'CC Cuts at: 3544 3544 Size: 6451 FspI TGC'GCA Cuts at: 4957 4957 Size: 6451 HaeII r_GCGC'y Cuts at: 1678 2173 2275 2338 3022 5598 1678 Size: 495 102 63 684 2576 2531 Fragments arranged by size: 2576 2531 684 495 102 63 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2872 2904 3167 3199 5092 Size: 32 135 32 1300 32 263 32 1893 Cuts at: 5092 5124 5166 5198 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1893 1300 263 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3795 4041 669 Size: 988 281 1857 246 3079 Fragments arranged by size: 3079 1857 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6451 HpaI GTT'AAC Cuts at: 4041 4041 Size: 6451 KasI G'GCGC_C Cuts at: 2271 2271 Size: 6451 KpnI G_GTAC'C Cuts at: 3776 3776 Size: 6451 MfeI C'AATT_G Cuts at: 4050 4050 Size: 6451 MluI A'CGCG_T Cuts at: 6327 6327 Size: 6451 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5565 5749 1305 Size: 4260 184 2007 Fragments arranged by size: 4260 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2984 3078 10 Size: 54 1425 343 1152 94 3383 Fragments arranged by size: 3383 1425 1152 343 94 54 MslI CAynn'nnrTG Cuts at: 518 1166 2754 2879 3587 4409 4768 4927 518 Size: 648 1588 125 708 822 359 159 2042 Fragments arranged by size: 2042 1588 822 708 648 359 159 125 MspA1I CmG'CkG Cuts at: 1606 2192 3867 4544 5692 5937 1606 Size: 586 1675 677 1148 245 2120 Fragments arranged by size: 2120 1675 1148 677 586 245 NaeI GCC'GGC Cuts at: 3542 3542 Size: 6451 NarI GG'CG_CC Cuts at: 2272 2272 Size: 6451 NcoI C'CATG_G Cuts at: 513 1065 2560 3697 513 Size: 552 1495 1137 3267 Fragments arranged by size: 3267 1495 1137 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6451 NgoMIV G'CCGG_C Cuts at: 3540 3540 Size: 6451 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6451 NotI GC'GGCC_GC Cuts at: 3898 3898 Size: 6451 NspI r_CATG'y Cuts at: 1132 1229 3809 5354 1132 Size: 97 2580 1545 2229 Fragments arranged by size: 2580 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2737 1348 Size: 1389 5062 PciI A'CATG_T Cuts at: 5350 5350 Size: 6451 PflMI CCAn_nnn'nTGG Cuts at: 1108 2737 2794 3052 1108 Size: 1629 57 258 4507 Fragments arranged by size: 4507 1629 258 57 PmeI GTTT'AAAC Cuts at: 3755 3755 Size: 6451 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2852 2884 3008 3040 4520 4552 6033 6065 2852 Size: 32 124 32 1480 32 1481 32 3238 Fragments arranged by size: 3238 1481 1480 124 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2149 3702 2149 Size: 1553 4898 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6451 PsiI TTA'TAA Cuts at: 4021 4021 Size: 6451 PspOMI G'GGCC_C Cuts at: 2948 2948 Size: 6451 PspXI vC'TCGA_Gb Cuts at: 2744 2744 Size: 6451 PstI C_TGCA'G Cuts at: 830 3803 830 Size: 2973 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6451 SacI G_AGCT'C Cuts at: 3330 3330 Size: 6451 SalI G'TCGA_C Cuts at: 1936 3793 1936 Size: 1857 4594 SanDI GG'GwC_CC Cuts at: 3702 3702 Size: 6451 SapI GCTCTTCn'nnn_ Cuts at: 3318 3318 Size: 6451 SbfI CC_TGCA'GG Cuts at: 3803 3803 Size: 6451 ScaI AGT'ACT Cuts at: 3308 4699 6411 3308 Size: 1391 1712 3348 Fragments arranged by size: 3348 1712 1391 SfcI C'TryA_G Cuts at: 826 1045 2120 3799 4934 5615 5806 826 Size: 219 1075 1679 1135 681 191 1471 Fragments arranged by size: 1679 1471 1135 1075 681 219 191 SfoI GGC'GCC Cuts at: 2273 2273 Size: 6451 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6451 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6451 SmaI CCC'GGG Cuts at: 1872 2146 3778 1872 Size: 274 1632 4545 Fragments arranged by size: 4545 1632 274 SmlI C'TyrA_G Cuts at: 820 1017 1943 2744 4555 5456 5718 820 Size: 197 926 801 1811 901 262 1553 Fragments arranged by size: 1811 1553 926 901 801 262 197 SnaBI TAC'GTA Cuts at: 493 2156 4242 493 Size: 1663 2086 2702 Fragments arranged by size: 2702 2086 1663 SpeI A'CTAG_T Cuts at: 152 152 Size: 6451 SphI G_CATG'C Cuts at: 1132 3809 1132 Size: 2677 3774 SspI AAT'ATT Cuts at: 5 52 4375 5 Size: 47 4323 2081 Fragments arranged by size: 4323 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6451 StyI C'CwwG_G Cuts at: 513 1065 2403 2560 2663 3697 3890 513 Size: 552 1338 157 103 1034 193 3074 Fragments arranged by size: 3074 1338 1034 552 193 157 103 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2657 3157 4478 4495 4648 4833 5278 Size: 1255 1077 500 1321 17 153 185 445 Cuts at: 5278 325 Size: 1498 Fragments arranged by size: 1498 1321 1255 1077 500 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3306 4697 6409 Size: 274 80 33 51 1443 1329 1391 1712 Cuts at: 6409 96 Size: 138 Fragments arranged by size: 1712 1443 1391 1329 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2463 2495 6411 6443 2463 Size: 32 3916 32 2471 Fragments arranged by size: 3916 2471 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3746 1316 Size: 2430 4021 XbaI T'CTAG_A Cuts at: 3787 3787 Size: 6451 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2849 3053 2849 Size: 204 6247 XhoI C'TCGA_G Cuts at: 1943 2744 1943 Size: 801 5650 XmaI C'CCGG_G Cuts at: 1870 2144 3776 1870 Size: 274 1632 4545 Fragments arranged by size: 4545 1632 274 XmnI GAAnn'nnTTC Cuts at: 4580 4580 Size: 6451 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4259 276 Size: 53 83 186 936 2725 2468 Fragments arranged by size: 2725 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BbvCI BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoRV FspAI NruI NsiI PacI PfoI PmlI PsrI PvuII RsrII SacII SexAI SfiI SrfI SwaI