(Circular) (Six-base) MAPSORT of: pFN21AB5091.seq Check: 8972 from: 1 to: 6517 pFN21AB5091 6517 bp With 182 enzymes: SgfI * October 20, 2009 10:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4327 278 Size: 53 83 186 936 2791 2468 Fragments arranged by size: 2791 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3860 1937 Size: 1923 4594 Acc65I G'GTAC_C Cuts at: 3838 3838 Size: 6517 AclI AA'CG_TT Cuts at: 1563 2262 3802 4644 5017 1563 Size: 699 1540 842 373 3063 Fragments arranged by size: 3063 1540 842 699 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3398 4580 5964 6232 1632 Size: 1766 1182 1384 268 1917 Fragments arranged by size: 1917 1766 1384 1182 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6320 AflIII A'CryG_T Cuts at: 1203 5416 6393 1203 Size: 4213 977 1327 Fragments arranged by size: 4213 1327 977 AgeI A'CCGG_T Cuts at: 5348 5348 Size: 6517 AhdI GACnn_n'nnGTC Cuts at: 5246 5246 Size: 6517 AleI CACnn'nnGTG Cuts at: 1166 3018 1166 Size: 1852 4665 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2080 1184 Size: 896 5621 AlwNI CAG_nnn'CTG Cuts at: 4260 5832 4260 Size: 1572 4945 ApaLI G'TGCA_C Cuts at: 2225 4574 5730 2225 Size: 2349 1156 3012 Fragments arranged by size: 3012 2349 1156 ApoI r'AATT_y Cuts at: 1493 2177 3041 3101 3150 3826 4052 4204 6313 Size: 684 864 60 49 676 226 152 2109 Cuts at: 6313 1493 Size: 1697 Fragments arranged by size: 2109 1697 864 684 676 226 152 60 49 AseI AT'TA_AT Cuts at: 160 1032 5071 160 Size: 872 4039 1606 Fragments arranged by size: 4039 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6517 AvaI C'yCGr_G Cuts at: 1870 1943 3842 1870 Size: 73 1899 4545 Fragments arranged by size: 4545 1899 73 BamHI G'GATC_C Cuts at: 3847 4214 3847 Size: 367 6150 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2845 3838 5293 618 Size: 325 217 627 1058 993 1455 1842 Fragments arranged by size: 1842 1455 1058 993 627 325 217 BanII G_rGCy'C Cuts at: 1391 2416 2543 3723 3836 1391 Size: 1025 127 1180 113 4072 Fragments arranged by size: 4072 1180 1025 127 113 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5546 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4708 4742 1903 Size: 34 50 34 2687 34 3678 Fragments arranged by size: 3678 2687 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2757 3483 4409 5619 2757 Size: 726 926 1210 3655 Fragments arranged by size: 3655 1210 926 726 BclI T'GATC_A Cuts at: 1548 1548 Size: 6517 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5128 136 Size: 107 122 71 631 605 3456 1525 Fragments arranged by size: 3456 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6512 6512 Size: 6517 BlpI GC'TnA_GC Cuts at: 2045 3934 2045 Size: 1889 4628 Bme1580I G_kGCm'C Cuts at: 1790 2229 4578 5734 1790 Size: 439 2349 1156 2573 Fragments arranged by size: 2573 2349 1156 439 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6517 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3217 4248 5206 Size: 416 53 83 399 24 1800 1031 958 Cuts at: 5206 442 Size: 1753 Fragments arranged by size: 1800 1753 1031 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6517 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5177 1239 Size: 3938 2579 Bpu10I CC'TnA_GC Cuts at: 2802 2802 Size: 6517 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2778 3037 4642 5507 5805 2778 Size: 259 1605 865 298 3490 Fragments arranged by size: 3490 1605 865 298 259 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5180 882 Size: 617 3681 2219 Fragments arranged by size: 3681 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4308 493 Size: 711 3104 2702 Fragments arranged by size: 3104 2702 711 BsaBI GATnn'nnATC Cuts at: 3777 6511 3777 Size: 2734 3783 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4324 4706 5341 275 Size: 53 83 186 936 2791 382 635 1451 Fragments arranged by size: 2791 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2173 3761 4950 5348 5622 5769 6180 2173 Size: 1588 1189 398 274 147 411 2510 Fragments arranged by size: 2510 1588 1189 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6292 Fragments arranged by size: 6292 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2781 1186 Size: 1595 4922 BseYI C'CCAG_C Cuts at: 2376 2568 2632 5720 6216 2376 Size: 192 64 3088 496 2677 Fragments arranged by size: 3088 2677 496 192 64 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5944 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3967 4728 5756 1540 Size: 275 186 1966 761 1028 2301 Fragments arranged by size: 2301 1966 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2229 3544 3723 4578 4663 5734 2229 Size: 1315 179 855 85 1071 3012 Fragments arranged by size: 3012 1315 1071 855 179 85 BsmI GAATG_Cn' Cuts at: 2558 4028 4121 2558 Size: 1470 93 4954 Fragments arranged by size: 4954 1470 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2229 2416 2543 3544 3723 3836 4578 Size: 399 439 187 127 1001 179 113 742 Cuts at: 4578 4663 5734 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 1001 742 439 399 187 179 127 113 85 BspEI T'CCGG_A Cuts at: 3761 6180 3761 Size: 2419 4098 BspHI T'CATG_A Cuts at: 3154 4406 6136 3154 Size: 1252 1730 3535 Fragments arranged by size: 3535 1730 1252 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3872 844 Size: 3028 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3759 4404 1394 Size: 429 1936 645 3507 Fragments arranged by size: 3507 1936 645 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5012 5186 65 Size: 1183 3764 174 1396 Fragments arranged by size: 3764 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5161 5348 1700 Size: 110 3351 187 2869 Fragments arranged by size: 3351 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6517 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6517 BssSI C'ACGA_G Cuts at: 4577 5589 4577 Size: 1012 5505 BstAPI GCAn_nnn'nTGC Cuts at: 794 2564 794 Size: 1770 4747 BstBI TT'CG_AA Cuts at: 2287 5321 2287 Size: 3034 3483 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6517 BstXI CCAn_nnnn'nTGG Cuts at: 2632 2632 Size: 6517 BstYI r'GATC_y Cuts at: 1972 3712 3847 4214 4599 4616 6068 6154 6166 Size: 1740 135 367 385 17 1452 86 12 Cuts at: 6166 6512 1972 Size: 346 1977 Fragments arranged by size: 1977 1740 1452 385 367 346 135 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2638 3525 513 Size: 552 1573 887 3505 Fragments arranged by size: 3505 1573 887 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2916 487 Size: 683 56 1690 4088 Fragments arranged by size: 4088 1690 683 56 BtsI GCAGTG_nn' Cuts at: 3642 4033 4827 4847 3642 Size: 391 794 20 5312 Fragments arranged by size: 5312 794 391 20 ClaI AT'CG_AT Cuts at: 1554 3456 1554 Size: 1902 4615 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6482 DraI TTT'AAA Cuts at: 3821 3821 Size: 6517 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5524 809 Size: 507 4208 1802 Fragments arranged by size: 4208 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3500 3964 4853 Size: 54 1425 50 275 18 1670 464 889 Cuts at: 4853 6482 8 Size: 1629 43 Fragments arranged by size: 1670 1629 1425 889 464 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3964 1537 Size: 275 2152 4090 Fragments arranged by size: 4090 2152 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4447 1353 Size: 3094 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5104 5478 5624 387 Size: 1064 138 3515 374 146 1280 Fragments arranged by size: 3515 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3721 3721 Size: 6517 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3398 4580 5177 5964 6232 1239 Size: 393 1766 1182 597 787 268 1524 Fragments arranged by size: 1766 1524 1182 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2702 2702 Size: 6517 EcoRI G'AATT_C Cuts at: 3826 4204 3826 Size: 378 6139 EcoRV GAT'ATC Cuts at: 3775 3775 Size: 6517 FspI TGC'GCA Cuts at: 5023 5023 Size: 6517 HaeII r_GCGC'y Cuts at: 1678 2045 5664 1678 Size: 367 3619 2531 Fragments arranged by size: 3619 2531 367 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2323 2355 2734 2766 2773 Size: 32 135 32 751 32 379 32 7 Cuts at: 2773 2805 3010 3042 5158 5190 5232 5264 1373 Size: 32 205 32 2116 32 42 32 2626 Fragments arranged by size: 2626 2116 751 379 205 135 42 32 32 32 32 32 32 32 32 7 HincII GTy'rAC Cuts at: 669 1657 1938 2313 3861 4107 669 Size: 988 281 375 1548 246 3079 Fragments arranged by size: 3079 1548 988 375 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6517 HpaI GTT'AAC Cuts at: 4107 4107 Size: 6517 KpnI G_GTAC'C Cuts at: 3842 3842 Size: 6517 MfeI C'AATT_G Cuts at: 4116 4116 Size: 6517 MluI A'CGCG_T Cuts at: 6393 6393 Size: 6517 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3414 3517 5631 5815 1305 Size: 2109 103 2114 184 2007 Fragments arranged by size: 2114 2109 2007 184 103 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4695 Fragments arranged by size: 4695 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3018 4475 4834 4993 518 Size: 648 1852 1457 359 159 2042 Fragments arranged by size: 2042 1852 1457 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3933 4610 5758 6003 1606 Size: 2327 677 1148 245 2120 Fragments arranged by size: 2327 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2638 3525 513 Size: 552 1573 887 3505 Fragments arranged by size: 3505 1573 887 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6517 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6517 NotI GC'GGCC_GC Cuts at: 3964 3964 Size: 6517 NspI r_CATG'y Cuts at: 1132 1229 3875 5420 1132 Size: 97 2646 1545 2229 Fragments arranged by size: 2646 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2536 1348 Size: 1188 5329 PciI A'CATG_T Cuts at: 5416 5416 Size: 6517 PflMI CCAn_nnn'nTGG Cuts at: 1108 2644 2874 1108 Size: 1536 230 4751 Fragments arranged by size: 4751 1536 230 PmeI GTTT'AAAC Cuts at: 3821 3821 Size: 6517 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2930 2962 4586 4618 6099 6131 2930 Size: 32 1624 32 1481 32 3316 Fragments arranged by size: 3316 1624 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6517 PsiI TTA'TAA Cuts at: 4087 4087 Size: 6517 PstI C_TGCA'G Cuts at: 830 3430 3869 830 Size: 2600 439 3478 Fragments arranged by size: 3478 2600 439 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6517 SacI G_AGCT'C Cuts at: 3723 3723 Size: 6517 SalI G'TCGA_C Cuts at: 1936 3859 1936 Size: 1923 4594 SbfI CC_TGCA'GG Cuts at: 3869 3869 Size: 6517 ScaI AGT'ACT Cuts at: 3789 4765 6477 3789 Size: 976 1712 3829 Fragments arranged by size: 3829 1712 976 SfcI C'TryA_G Cuts at: 826 1045 3426 3865 5000 5681 5872 826 Size: 219 2381 439 1135 681 191 1471 Fragments arranged by size: 2381 1471 1135 681 439 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6517 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6517 SmaI CCC'GGG Cuts at: 1872 3844 1872 Size: 1972 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2793 3016 4621 5522 5784 820 Size: 197 926 850 223 1605 901 262 1553 Fragments arranged by size: 1605 1553 926 901 850 262 223 197 SnaBI TAC'GTA Cuts at: 493 4308 493 Size: 3815 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6517 SphI G_CATG'C Cuts at: 1132 3875 1132 Size: 2743 3774 SspI AAT'ATT Cuts at: 5 52 4441 5 Size: 47 4389 2081 Fragments arranged by size: 4389 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6517 StyI C'CwwG_G Cuts at: 513 1065 2448 2638 3525 3956 513 Size: 552 1383 190 887 431 3074 Fragments arranged by size: 3074 1383 887 552 431 190 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2091 2441 4544 4561 4714 4899 5344 Size: 1255 511 350 2103 17 153 185 445 Cuts at: 5344 325 Size: 1498 Fragments arranged by size: 2103 1498 1255 511 445 350 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3292 3787 4763 Size: 274 80 33 51 1443 1315 495 976 Cuts at: 4763 6475 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1315 976 495 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6477 6509 6477 Size: 32 6485 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6517 XbaI T'CTAG_A Cuts at: 3853 3853 Size: 6517 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6517 XmaI C'CCGG_G Cuts at: 1870 3842 1870 Size: 1972 4545 XmnI GAAnn'nnTTC Cuts at: 4646 4646 Size: 6517 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4325 276 Size: 53 83 186 936 2791 2468 Fragments arranged by size: 2791 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI