(Circular) (Six-base) MAPSORT of: pFN21AB5086.seq Check: 8794 from: 1 to: 6685 pFN21AB5086 6685 bp With 182 enzymes: SgfI * February 27, 2009 14:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4495 278 Size: 53 83 186 936 2959 2468 Fragments arranged by size: 2959 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3755 4028 1937 Size: 1818 273 4594 Fragments arranged by size: 4594 1818 273 Acc65I G'GTAC_C Cuts at: 4006 4006 Size: 6685 AclI AA'CG_TT Cuts at: 1563 3078 4812 5185 1563 Size: 1515 1734 373 3063 Fragments arranged by size: 3063 1734 1515 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2963 2984 3022 3125 3392 4748 6132 6400 Size: 1331 21 38 103 267 1356 1384 268 Cuts at: 6400 1632 Size: 1917 Fragments arranged by size: 1917 1384 1356 1331 268 267 103 38 21 AfeI AGC'GCT Cuts at: 3386 3386 Size: 6685 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6488 AflIII A'CryG_T Cuts at: 1203 2910 5584 6561 1203 Size: 1707 2674 977 1327 Fragments arranged by size: 2674 1707 1327 977 AgeI A'CCGG_T Cuts at: 5516 5516 Size: 6685 AhdI GACnn_n'nnGTC Cuts at: 5414 5414 Size: 6685 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6685 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3341 3885 1184 Size: 2157 544 3984 Fragments arranged by size: 3984 2157 544 AlwNI CAG_nnn'CTG Cuts at: 2959 3646 3981 4428 6000 2959 Size: 687 335 447 1572 3644 Fragments arranged by size: 3644 1572 687 447 335 ApaI G_GGCC'C Cuts at: 3269 3770 3797 3269 Size: 501 27 6157 Fragments arranged by size: 6157 501 27 ApaLI G'TGCA_C Cuts at: 3515 4742 5898 3515 Size: 1227 1156 4302 Fragments arranged by size: 4302 1227 1156 ApoI r'AATT_y Cuts at: 1493 3994 4220 4372 6481 1493 Size: 2501 226 152 2109 1697 Fragments arranged by size: 2501 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5239 160 Size: 872 4207 1606 Fragments arranged by size: 4207 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6685 AvaI C'yCGr_G Cuts at: 1870 1943 2039 2053 3193 4010 1870 Size: 73 96 14 1140 817 4545 Fragments arranged by size: 4545 1140 817 96 73 14 BamHI G'GATC_C Cuts at: 4015 4382 4015 Size: 367 6318 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3680 4006 5461 618 Size: 325 217 627 1893 326 1455 1842 Fragments arranged by size: 1893 1842 1455 627 326 325 217 BanII G_rGCy'C Cuts at: 1391 2046 2186 3269 3770 3797 4004 1391 Size: 655 140 1083 501 27 207 4072 Fragments arranged by size: 4072 1083 655 501 207 140 27 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2761 928 Size: 971 862 4852 Fragments arranged by size: 4852 971 862 BbvCI CC'TCA_GC Cuts at: 3891 3891 Size: 6685 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4876 4910 1903 Size: 34 2939 34 3678 Fragments arranged by size: 3678 2939 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4577 5787 4577 Size: 1210 5475 BclI T'GATC_A Cuts at: 1548 3237 1548 Size: 1689 4996 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2075 2673 5296 Size: 107 122 71 631 605 403 598 2623 Cuts at: 5296 136 Size: 1525 Fragments arranged by size: 2623 1525 631 605 598 403 122 107 71 BglII A'GATC_T Cuts at: 2694 3069 3097 6680 2694 Size: 375 28 3583 2699 Fragments arranged by size: 3583 2699 375 28 BlpI GC'TnA_GC Cuts at: 4102 4102 Size: 6685 Bme1580I G_kGCm'C Cuts at: 1790 3059 3269 3519 3770 3797 4746 5902 1790 Size: 1269 210 250 251 27 949 1156 2573 Fragments arranged by size: 2573 1269 1156 949 251 250 210 27 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6685 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3588 4416 5374 Size: 416 53 83 399 24 2171 828 958 Cuts at: 5374 442 Size: 1753 Fragments arranged by size: 2171 1753 958 828 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6685 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2065 2065 Size: 6685 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2474 2702 3683 3852 3869 5345 1239 Size: 1235 228 981 169 17 1476 2579 Fragments arranged by size: 2579 1476 1235 981 228 169 17 Bpu10I CC'TnA_GC Cuts at: 3891 3897 3891 Size: 6 6679 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4810 5675 5973 4810 Size: 865 298 5522 Fragments arranged by size: 5522 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2246 5348 882 Size: 617 747 3102 2219 Fragments arranged by size: 3102 2219 747 617 BsaAI yAC'GTr Cuts at: 493 1204 4476 493 Size: 711 3272 2702 Fragments arranged by size: 3272 2702 711 BsaBI GATnn'nnATC Cuts at: 6679 6679 Size: 6685 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4492 4874 5509 275 Size: 53 83 186 936 2959 382 635 1451 Fragments arranged by size: 2959 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2424 3396 5118 5516 5790 5937 6348 2424 Size: 972 1722 398 274 147 411 2761 Fragments arranged by size: 2761 1722 972 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3110 3140 3170 3200 1178 Size: 30 165 30 1707 30 30 30 4663 Fragments arranged by size: 4663 1707 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3113 3164 3167 3686 3835 1186 Size: 1927 51 3 519 149 4036 Fragments arranged by size: 4036 1927 519 149 51 3 BseYI C'CCAG_C Cuts at: 2045 2396 3262 3902 5888 6384 2045 Size: 351 866 640 1986 496 2346 Fragments arranged by size: 2346 1986 866 640 496 351 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3020 3614 1163 Size: 573 1284 594 4234 Fragments arranged by size: 4234 1284 594 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2079 4135 4896 5924 1540 Size: 275 186 78 2056 761 1028 2301 Fragments arranged by size: 2301 2056 1028 761 275 186 78 BsiHKAI G_wGCw'C Cuts at: 3519 3823 4746 4831 5902 3519 Size: 304 923 85 1071 4302 Fragments arranged by size: 4302 1071 923 304 85 BsmI GAATG_Cn' Cuts at: 4196 4289 4196 Size: 93 6592 BsmBI CGTCTCn'nnnn_ Cuts at: 3777 3777 Size: 6685 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2046 2186 3059 3269 3519 3770 3797 Size: 399 256 140 873 210 250 251 27 Cuts at: 3797 3823 4004 4746 4831 5902 1391 Size: 26 181 742 85 1071 2174 Fragments arranged by size: 2174 1071 873 742 399 256 251 250 210 181 140 85 27 26 BspEI T'CCGG_A Cuts at: 2424 3396 6348 2424 Size: 972 2952 2761 Fragments arranged by size: 2952 2761 972 BspHI T'CATG_A Cuts at: 2743 4574 6304 2743 Size: 1831 1730 3124 Fragments arranged by size: 3124 1831 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3636 4040 844 Size: 2792 404 3489 Fragments arranged by size: 3489 2792 404 BsrBI CCG'CTC Cuts at: 1394 1823 4572 1394 Size: 429 2749 3507 Fragments arranged by size: 3507 2749 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2650 2827 3294 5180 5354 65 Size: 1183 1402 177 467 1886 174 1396 Fragments arranged by size: 1886 1402 1396 1183 467 177 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2014 3915 5329 5516 1700 Size: 110 204 1901 1414 187 2869 Fragments arranged by size: 2869 1901 1414 204 187 110 BsrGI T'GTAC_A Cuts at: 96 3595 96 Size: 3499 3186 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6685 BssSI C'ACGA_G Cuts at: 4745 5757 4745 Size: 1012 5673 BstAPI GCAn_nnn'nTGC Cuts at: 794 3365 794 Size: 2571 4114 BstBI TT'CG_AA Cuts at: 5489 5489 Size: 6685 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6685 BstXI CCAn_nnnn'nTGG Cuts at: 2170 2608 2170 Size: 438 6247 BstYI r'GATC_y Cuts at: 1972 2694 2985 3069 3097 4015 4382 4767 4784 Size: 722 291 84 28 918 367 385 17 Cuts at: 4784 6236 6322 6334 6680 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 918 722 385 367 346 291 86 84 28 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2070 2092 2668 3510 513 Size: 552 939 66 22 576 842 3688 Fragments arranged by size: 3688 939 842 576 552 66 22 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3299 487 Size: 683 56 2073 3873 Fragments arranged by size: 3873 2073 683 56 BtsI GCAGTG_nn' Cuts at: 2475 2611 3913 4201 4995 5015 2475 Size: 136 1302 288 794 20 4145 Fragments arranged by size: 4145 1302 794 288 136 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6685 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6650 DraI TTT'AAA Cuts at: 3989 3989 Size: 6685 DraIII CAC_nnn'GTG Cuts at: 3943 3943 Size: 6685 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2015 5692 809 Size: 507 699 3677 1802 Fragments arranged by size: 3677 1802 699 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2061 2076 2539 Size: 54 1425 50 275 18 231 15 463 Cuts at: 2539 3349 4132 5021 6650 8 Size: 810 783 889 1629 43 Fragments arranged by size: 1629 1425 889 810 783 463 275 231 54 50 43 18 15 EagI C'GGCC_G Cuts at: 1537 1812 2076 4132 1537 Size: 275 264 2056 4090 Fragments arranged by size: 4090 2056 275 264 EarI CTCTTCn'nnn_ Cuts at: 1353 2333 2570 4615 1353 Size: 980 237 2045 3423 Fragments arranged by size: 3423 2045 980 237 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2363 2558 5272 5646 5792 387 Size: 1064 138 774 195 2714 374 146 1280 Fragments arranged by size: 2714 1280 1064 774 374 195 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2474 2702 2963 2984 3022 3125 3392 Size: 393 842 228 261 21 38 103 267 Cuts at: 3392 3683 3852 3869 4748 5345 6132 6400 1239 Size: 291 169 17 879 597 787 268 1524 Fragments arranged by size: 1524 879 842 787 597 393 291 268 267 261 228 169 103 38 21 17 EcoO109I rG'GnC_Cy Cuts at: 2512 3766 3767 3793 3957 2512 Size: 1254 1 26 164 5240 Fragments arranged by size: 5240 1254 164 26 1 EcoRI G'AATT_C Cuts at: 3994 4372 3994 Size: 378 6307 FspI TGC'GCA Cuts at: 3575 5191 3575 Size: 1616 5069 HaeII r_GCGC'y Cuts at: 1678 2135 2959 3388 3937 5832 1678 Size: 457 824 429 549 1895 2531 Fragments arranged by size: 2531 1895 824 549 457 429 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2770 2802 3170 3202 3222 Size: 32 135 32 1198 32 368 32 20 Cuts at: 3222 3254 3827 3859 5326 5358 5400 5432 1373 Size: 32 573 32 1467 32 42 32 2626 Fragments arranged by size: 2626 1467 1198 573 368 135 42 32 32 32 32 32 32 32 32 20 HincII GTy'rAC Cuts at: 669 1657 1938 3756 4029 4275 669 Size: 988 281 1818 273 246 3079 Fragments arranged by size: 3079 1818 988 281 273 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6685 HpaI GTT'AAC Cuts at: 4275 4275 Size: 6685 KpnI G_GTAC'C Cuts at: 4010 4010 Size: 6685 MfeI C'AATT_G Cuts at: 4284 4284 Size: 6685 MluI A'CGCG_T Cuts at: 6561 6561 Size: 6685 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3057 3099 5799 5983 1305 Size: 1752 42 2700 184 2007 Fragments arranged by size: 2700 2007 1752 184 42 MscI TGG'CCA Cuts at: 10 64 1489 1832 3351 10 Size: 54 1425 343 1519 3344 Fragments arranged by size: 3344 1519 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2577 2807 3410 4643 5002 5161 518 Size: 648 1411 230 603 1233 359 159 2042 Fragments arranged by size: 2042 1411 1233 648 603 359 230 159 MspA1I CmG'CkG Cuts at: 1606 2072 3643 3869 3978 4101 4778 5926 6171 Size: 466 1571 226 109 123 677 1148 245 Cuts at: 6171 1606 Size: 2120 Fragments arranged by size: 2120 1571 1148 677 466 245 226 123 109 NaeI GCC'GGC Cuts at: 3917 3917 Size: 6685 NcoI C'CATG_G Cuts at: 513 1065 2004 3510 513 Size: 552 939 1506 3688 Fragments arranged by size: 3688 1506 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6685 NgoMIV G'CCGG_C Cuts at: 3915 3915 Size: 6685 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6685 NotI GC'GGCC_GC Cuts at: 2076 4132 2076 Size: 2056 4629 NspI r_CATG'y Cuts at: 1132 1229 2806 2914 3328 4043 5588 1132 Size: 97 1577 108 414 715 1545 2229 Fragments arranged by size: 2229 1577 1545 715 414 108 97 PasI CC'CwG_GG Cuts at: 1348 3952 1348 Size: 2604 4081 PciI A'CATG_T Cuts at: 2910 5584 2910 Size: 2674 4011 PflMI CCAn_nnn'nTGG Cuts at: 1108 3511 3646 1108 Size: 2403 135 4147 Fragments arranged by size: 4147 2403 135 PfoI T'CCnGG_A Cuts at: 2451 3654 2451 Size: 1203 5482 PmeI GTTT'AAAC Cuts at: 3989 3989 Size: 6685 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4754 4786 6267 6299 4754 Size: 32 1481 32 5140 Fragments arranged by size: 5140 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2512 2512 Size: 6685 PshAI GACnn'nnGTC Cuts at: 1613 2913 1613 Size: 1300 5385 PsiI TTA'TAA Cuts at: 4255 4255 Size: 6685 PspOMI G'GGCC_C Cuts at: 3265 3766 3793 3265 Size: 501 27 6157 Fragments arranged by size: 6157 501 27 PstI C_TGCA'G Cuts at: 830 2117 4037 830 Size: 1287 1920 3478 Fragments arranged by size: 3478 1920 1287 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6685 PvuII CAG'CTG Cuts at: 3869 3869 Size: 6685 SacII CC_GC'GG Cuts at: 2073 2073 Size: 6685 SalI G'TCGA_C Cuts at: 1936 3754 4027 1936 Size: 1818 273 4594 Fragments arranged by size: 4594 1818 273 SapI GCTCTTCn'nnn_ Cuts at: 2333 2333 Size: 6685 SbfI CC_TGCA'GG Cuts at: 4037 4037 Size: 6685 ScaI AGT'ACT Cuts at: 4933 6645 4933 Size: 1712 4973 SexAI A'CCwGG_T Cuts at: 2418 2418 Size: 6685 SfcI C'TryA_G Cuts at: 826 1045 2113 3142 3849 4033 5168 5849 6040 Size: 219 1068 1029 707 184 1135 681 191 Cuts at: 6040 826 Size: 1471 Fragments arranged by size: 1471 1135 1068 1029 707 681 219 191 184 SfiI GGCCn_nnn'nGGCC Cuts at: 2075 2075 Size: 6685 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6685 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6685 SmaI CCC'GGG Cuts at: 1872 2055 3195 4012 1872 Size: 183 1140 817 4545 Fragments arranged by size: 4545 1140 817 183 SmlI C'TyrA_G Cuts at: 820 1017 1943 4789 5690 5952 820 Size: 197 926 2846 901 262 1553 Fragments arranged by size: 2846 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4476 493 Size: 3983 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6685 SphI G_CATG'C Cuts at: 1132 2806 3328 4043 1132 Size: 1674 522 715 3774 Fragments arranged by size: 3774 1674 715 522 SspI AAT'ATT Cuts at: 5 52 4609 5 Size: 47 4557 2081 Fragments arranged by size: 4557 2081 47 StuI AGG'CCT Cuts at: 1517 2321 3234 1517 Size: 804 913 4968 Fragments arranged by size: 4968 913 804 StyI C'CwwG_G Cuts at: 513 1065 2004 3276 3510 3669 4124 513 Size: 552 939 1272 234 159 455 3074 Fragments arranged by size: 3074 1272 939 552 455 234 159 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4712 4729 4882 5067 5512 325 Size: 1255 3132 17 153 185 445 1498 Fragments arranged by size: 3132 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3595 4931 6643 Size: 274 80 33 51 1443 1618 1336 1712 Cuts at: 6643 96 Size: 138 Fragments arranged by size: 1712 1618 1443 1336 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6645 6677 6645 Size: 32 6653 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6685 XbaI T'CTAG_A Cuts at: 4021 4021 Size: 6685 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2851 3553 2851 Size: 702 5983 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6685 XmaI C'CCGG_G Cuts at: 1870 2053 3193 4010 1870 Size: 183 1140 817 4545 Fragments arranged by size: 4545 1140 817 183 XmnI GAAnn'nnTTC Cuts at: 2564 3110 4814 2564 Size: 546 1704 4435 Fragments arranged by size: 4435 1704 546 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4493 276 Size: 53 83 186 936 2959 2468 Fragments arranged by size: 2959 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SanDI SfoI SrfI SwaI